Pre-mRNA-processing factor 19

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

WD-repeat protein

Species:

Leishmania major

UniProt:

E9ACW8_LEIMA

TriTrypDb:

LmjF.27.2480 , LMJLV39_270032600 , LMJSD75_270032500

Length:

513

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0000974	Prp19 complex	2	10
GO:0005654	nucleoplasm	2	2
GO:0005681	spliceosomal complex	3	10
GO:0005684	U2-type spliceosomal complex	4	2
GO:0005730	nucleolus	5	2
GO:0005737	cytoplasm	2	2
GO:0005840	ribosome	5	6
GO:0032991	protein-containing complex	1	10
GO:0043226	organelle	2	6
GO:0043228	non-membrane-bounded organelle	3	6
GO:0043229	intracellular organelle	3	6
GO:0043232	intracellular non-membrane-bounded organelle	4	6
GO:0071006	U2-type catalytic step 1 spliceosome	4	2
GO:0071012	catalytic step 1 spliceosome	3	2
GO:0110165	cellular anatomical entity	1	6
GO:0140513	nuclear protein-containing complex	2	10
GO:1902494	catalytic complex	2	2
GO:1990904	ribonucleoprotein complex	2	10

Expansion

Sequence features

E9ACW8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ACW8

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	10
GO:0000375	RNA splicing, via transesterification reactions	8	10
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	10
GO:0000398	mRNA splicing, via spliceosome	8	10
GO:0006139	nucleobase-containing compound metabolic process	3	10
GO:0006259	DNA metabolic process	4	10
GO:0006281	DNA repair	5	10
GO:0006396	RNA processing	6	10
GO:0006397	mRNA processing	7	10
GO:0006725	cellular aromatic compound metabolic process	3	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0006950	response to stress	2	10
GO:0006974	DNA damage response	4	10
GO:0008152	metabolic process	1	10
GO:0008380	RNA splicing	7	10
GO:0009987	cellular process	1	10
GO:0016070	RNA metabolic process	5	10
GO:0016071	mRNA metabolic process	6	10
GO:0016567	protein ubiquitination	7	10
GO:0019538	protein metabolic process	3	10
GO:0032446	protein modification by small protein conjugation	6	10
GO:0033554	cellular response to stress	3	10
GO:0034641	cellular nitrogen compound metabolic process	3	10
GO:0036211	protein modification process	4	10
GO:0043170	macromolecule metabolic process	3	10
GO:0043412	macromolecule modification	4	10
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	10
GO:0044260	obsolete cellular macromolecule metabolic process	3	10
GO:0046483	heterocycle metabolic process	3	10
GO:0050896	response to stimulus	1	10
GO:0051716	cellular response to stimulus	2	10
GO:0070534	protein K63-linked ubiquitination	9	10
GO:0070647	protein modification by small protein conjugation or removal	5	10
GO:0071704	organic substance metabolic process	2	10
GO:0090304	nucleic acid metabolic process	4	10
GO:1901360	organic cyclic compound metabolic process	3	10
GO:1901564	organonitrogen compound metabolic process	3	10

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0004842	ubiquitin-protein transferase activity	4	10
GO:0016740	transferase activity	2	10
GO:0019787	ubiquitin-like protein transferase activity	3	10
GO:0061630	ubiquitin protein ligase activity	5	10
GO:0061659	ubiquitin-like protein ligase activity	4	10
GO:0140096	catalytic activity, acting on a protein	2	10
GO:0016746	acyltransferase activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	156	158	PF00675	0.421
CLV_NRD_NRD_1	168	170	PF00675	0.380
CLV_NRD_NRD_1	171	173	PF00675	0.416
CLV_NRD_NRD_1	46	48	PF00675	0.430
CLV_PCSK_KEX2_1	156	158	PF00082	0.439
CLV_PCSK_SKI1_1	418	422	PF00082	0.473
DEG_APCC_DBOX_1	417	425	PF00400	0.435
DEG_Nend_Nbox_1	1	3	PF02207	0.554
DEG_SCF_FBW7_1	193	199	PF00400	0.394
DOC_ANK_TNKS_1	234	241	PF00023	0.394
DOC_ANK_TNKS_1	438	445	PF00023	0.466
DOC_CKS1_1	193	198	PF01111	0.386
DOC_MAPK_DCC_7	418	426	PF00069	0.440
DOC_MAPK_HePTP_8	6	18	PF00069	0.468
DOC_MAPK_MEF2A_6	9	18	PF00069	0.465
DOC_USP7_MATH_1	14	18	PF00917	0.345
DOC_USP7_MATH_1	175	179	PF00917	0.590
DOC_USP7_MATH_1	181	185	PF00917	0.493
DOC_USP7_MATH_1	194	198	PF00917	0.463
DOC_USP7_MATH_1	200	204	PF00917	0.502
DOC_USP7_MATH_1	215	219	PF00917	0.643
DOC_USP7_MATH_1	351	355	PF00917	0.350
DOC_USP7_MATH_1	59	63	PF00917	0.653
DOC_WW_Pin1_4	179	184	PF00397	0.624
DOC_WW_Pin1_4	192	197	PF00397	0.400
DOC_WW_Pin1_4	234	239	PF00397	0.455
LIG_14-3-3_CanoR_1	275	284	PF00244	0.531
LIG_14-3-3_CanoR_1	3	11	PF00244	0.457
LIG_14-3-3_CanoR_1	461	469	PF00244	0.548
LIG_14-3-3_CanoR_1	96	106	PF00244	0.542
LIG_Actin_WH2_2	116	133	PF00022	0.489
LIG_Actin_WH2_2	465	480	PF00022	0.478
LIG_BIR_III_2	432	436	PF00653	0.416
LIG_BRCT_BRCA1_1	219	223	PF00533	0.487
LIG_BRCT_BRCA1_1	392	396	PF00533	0.438
LIG_FHA_1	10	16	PF00498	0.484
LIG_FHA_1	249	255	PF00498	0.504
LIG_FHA_1	271	277	PF00498	0.421
LIG_FHA_1	330	336	PF00498	0.492
LIG_FHA_1	472	478	PF00498	0.455
LIG_FHA_1	490	496	PF00498	0.312
LIG_FHA_2	317	323	PF00498	0.497
LIG_FHA_2	343	349	PF00498	0.472
LIG_FHA_2	453	459	PF00498	0.302
LIG_FHA_2	465	471	PF00498	0.471
LIG_FHA_2	74	80	PF00498	0.504
LIG_LIR_LC3C_4	446	449	PF02991	0.381
LIG_LIR_Nem_3	84	88	PF02991	0.441
LIG_Rb_LxCxE_1	339	354	PF01857	0.459
LIG_RPA_C_Fungi	201	213	PF08784	0.434
LIG_SH2_CRK	352	356	PF00017	0.332
LIG_SH2_NCK_1	506	510	PF00017	0.462
LIG_SH2_STAP1	352	356	PF00017	0.444
LIG_SH2_STAP1	506	510	PF00017	0.471
LIG_SH2_STAT5	32	35	PF00017	0.393
LIG_SH2_STAT5	413	416	PF00017	0.343
LIG_SH3_3	145	151	PF00018	0.469
LIG_SH3_3	190	196	PF00018	0.444
LIG_SH3_3	417	423	PF00018	0.425
LIG_SH3_3	8	14	PF00018	0.389
LIG_SUMO_SIM_par_1	14	20	PF11976	0.469
LIG_SUMO_SIM_par_1	315	322	PF11976	0.453
LIG_SUMO_SIM_par_1	332	341	PF11976	0.323
LIG_TRAF2_1	137	140	PF00917	0.624
MOD_CK1_1	17	23	PF00069	0.356
MOD_CK1_1	184	190	PF00069	0.552
MOD_CK1_1	198	204	PF00069	0.608
MOD_CK1_1	225	231	PF00069	0.445
MOD_CK1_1	300	306	PF00069	0.448
MOD_CK1_1	463	469	PF00069	0.585
MOD_CK1_1	62	68	PF00069	0.522
MOD_CK1_1	73	79	PF00069	0.219
MOD_CK2_1	24	30	PF00069	0.357
MOD_CK2_1	264	270	PF00069	0.378
MOD_CK2_1	318	324	PF00069	0.429
MOD_CK2_1	342	348	PF00069	0.444
MOD_CK2_1	464	470	PF00069	0.518
MOD_CK2_1	73	79	PF00069	0.334
MOD_GlcNHglycan	19	22	PF01048	0.380
MOD_GlcNHglycan	198	201	PF01048	0.613
MOD_GlcNHglycan	203	206	PF01048	0.535
MOD_GlcNHglycan	219	222	PF01048	0.385
MOD_GlcNHglycan	224	227	PF01048	0.430
MOD_GlcNHglycan	266	269	PF01048	0.267
MOD_GlcNHglycan	293	296	PF01048	0.442
MOD_GlcNHglycan	324	327	PF01048	0.498
MOD_GlcNHglycan	340	343	PF01048	0.348
MOD_GlcNHglycan	392	395	PF01048	0.331
MOD_GlcNHglycan	396	399	PF01048	0.285
MOD_GlcNHglycan	429	432	PF01048	0.516
MOD_GlcNHglycan	462	465	PF01048	0.547
MOD_GlcNHglycan	479	482	PF01048	0.412
MOD_GlcNHglycan	489	492	PF01048	0.506
MOD_GlcNHglycan	65	68	PF01048	0.533
MOD_GSK3_1	175	182	PF00069	0.588
MOD_GSK3_1	192	199	PF00069	0.542
MOD_GSK3_1	200	207	PF00069	0.524
MOD_GSK3_1	282	289	PF00069	0.511
MOD_GSK3_1	318	325	PF00069	0.407
MOD_GSK3_1	329	336	PF00069	0.372
MOD_GSK3_1	338	345	PF00069	0.406
MOD_GSK3_1	382	389	PF00069	0.458
MOD_GSK3_1	390	397	PF00069	0.295
MOD_GSK3_1	460	467	PF00069	0.482
MOD_GSK3_1	485	492	PF00069	0.402
MOD_GSK3_1	59	66	PF00069	0.546
MOD_N-GLC_1	4	9	PF02516	0.456
MOD_NEK2_1	24	29	PF00069	0.448
MOD_NEK2_1	337	342	PF00069	0.533
MOD_NEK2_1	462	467	PF00069	0.519
MOD_NEK2_1	477	482	PF00069	0.288
MOD_NEK2_1	505	510	PF00069	0.436
MOD_NEK2_2	14	19	PF00069	0.469
MOD_PIKK_1	181	187	PF00454	0.468
MOD_PIKK_1	256	262	PF00454	0.297
MOD_PIKK_1	297	303	PF00454	0.439
MOD_PIKK_1	4	10	PF00454	0.411
MOD_PIKK_1	97	103	PF00454	0.354
MOD_PKA_2	121	127	PF00069	0.394
MOD_PKA_2	382	388	PF00069	0.501
MOD_PKA_2	460	466	PF00069	0.447
MOD_Plk_1	24	30	PF00069	0.357
MOD_Plk_1	270	276	PF00069	0.283
MOD_Plk_1	59	65	PF00069	0.575
MOD_Plk_2-3	239	245	PF00069	0.422
MOD_Plk_4	282	288	PF00069	0.563
MOD_Plk_4	374	380	PF00069	0.453
MOD_Plk_4	408	414	PF00069	0.257
MOD_Plk_4	452	458	PF00069	0.426
MOD_Plk_4	73	79	PF00069	0.374
MOD_ProDKin_1	179	185	PF00069	0.619
MOD_ProDKin_1	192	198	PF00069	0.414
MOD_ProDKin_1	234	240	PF00069	0.467
TRG_ENDOCYTIC_2	352	355	PF00928	0.365
TRG_ER_diArg_1	155	157	PF00400	0.516
TRG_NLS_Bipartite_1	156	173	PF00514	0.438
TRG_NLS_MonoExtC_3	168	173	PF00514	0.468
TRG_NLS_MonoExtN_4	166	173	PF00514	0.462
TRG_Pf-PMV_PEXEL_1	26	30	PF00026	0.319

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMA9	Leptomonas seymouri	64%	100%
A0A0S4J7B3	Bodo saltans	34%	100%
A0A1X0NFA4	Trypanosomatidae	35%	100%
A0A3Q8IGJ0	Leishmania donovani	98%	100%
A0A3R7NBE4	Trypanosoma rangeli	35%	100%
A4HG28	Leishmania braziliensis	84%	99%
A4I350	Leishmania infantum	98%	100%
C9ZJM1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
E9AZF7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
O22785	Arabidopsis thaliana	28%	98%
Q08E38	Bos taurus	31%	100%
Q10051	Caenorhabditis elegans	26%	100%
Q5ZMA2	Gallus gallus	31%	100%
Q94BR4	Arabidopsis thaliana	26%	98%
Q99KP6	Mus musculus	31%	100%
Q9AV81	Oryza sativa subsp. japonica	27%	97%
Q9JMJ4	Rattus norvegicus	31%	100%
Q9UMS4	Homo sapiens	31%	100%