Putative dual specificity protein phosphatase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

kinetoplastid-specific dual specificity phosphatase, putative

Species:

Leishmania major

UniProt:

E9ACT8_LEIMA

TriTrypDb:

LmjF.27.2210 , LMJLV39_270029500 , LMJSD75_270029400

Length:

352

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005829	cytosol	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9ACT8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ACT8

Function

Biological processes
Term	Name	Level	Count
GO:0006470	protein dephosphorylation	5	11
GO:0006793	phosphorus metabolic process	3	11
GO:0006796	phosphate-containing compound metabolic process	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0007165	signal transduction	2	2
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016311	dephosphorylation	5	11
GO:0019538	protein metabolic process	3	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0065007	biological regulation	1	2
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004721	phosphoprotein phosphatase activity	3	11
GO:0004725	protein tyrosine phosphatase activity	4	2
GO:0008138	protein tyrosine/serine/threonine phosphatase activity	4	11
GO:0016787	hydrolase activity	2	11
GO:0016788	hydrolase activity, acting on ester bonds	3	11
GO:0016791	phosphatase activity	5	11
GO:0042578	phosphoric ester hydrolase activity	4	11
GO:0140096	catalytic activity, acting on a protein	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	116	118	PF00675	0.580
CLV_NRD_NRD_1	12	14	PF00675	0.780
CLV_NRD_NRD_1	286	288	PF00675	0.410
CLV_NRD_NRD_1	334	336	PF00675	0.696
CLV_NRD_NRD_1	350	352	PF00675	0.486
CLV_NRD_NRD_1	67	69	PF00675	0.716
CLV_PCSK_KEX2_1	116	118	PF00082	0.559
CLV_PCSK_KEX2_1	12	14	PF00082	0.539
CLV_PCSK_KEX2_1	208	210	PF00082	0.306
CLV_PCSK_KEX2_1	334	336	PF00082	0.718
CLV_PCSK_KEX2_1	350	352	PF00082	0.464
CLV_PCSK_KEX2_1	67	69	PF00082	0.781
CLV_PCSK_PC1ET2_1	208	210	PF00082	0.306
CLV_PCSK_PC7_1	204	210	PF00082	0.306
CLV_PCSK_SKI1_1	142	146	PF00082	0.593
DOC_CKS1_1	219	224	PF01111	0.306
DOC_CKS1_1	85	90	PF01111	0.425
DOC_CYCLIN_RxL_1	137	149	PF00134	0.512
DOC_CYCLIN_RxL_1	26	35	PF00134	0.455
DOC_CYCLIN_RxL_1	284	294	PF00134	0.357
DOC_MAPK_gen_1	137	145	PF00069	0.426
DOC_PP1_RVXF_1	27	34	PF00149	0.455
DOC_USP7_MATH_1	3	7	PF00917	0.684
DOC_USP7_MATH_1	338	342	PF00917	0.565
DOC_WW_Pin1_4	218	223	PF00397	0.488
DOC_WW_Pin1_4	242	247	PF00397	0.441
DOC_WW_Pin1_4	305	310	PF00397	0.279
DOC_WW_Pin1_4	53	58	PF00397	0.793
DOC_WW_Pin1_4	84	89	PF00397	0.619
LIG_14-3-3_CanoR_1	116	120	PF00244	0.555
LIG_14-3-3_CanoR_1	173	179	PF00244	0.601
LIG_14-3-3_CanoR_1	209	219	PF00244	0.298
LIG_14-3-3_CanoR_1	290	300	PF00244	0.340
LIG_14-3-3_CanoR_1	32	40	PF00244	0.413
LIG_14-3-3_CterR_2	350	352	PF00244	0.501
LIG_FHA_1	134	140	PF00498	0.454
LIG_FHA_1	43	49	PF00498	0.694
LIG_FHA_1	85	91	PF00498	0.591
LIG_FHA_2	116	122	PF00498	0.417
LIG_FHA_2	200	206	PF00498	0.234
LIG_LIR_Gen_1	228	236	PF02991	0.281
LIG_LIR_Gen_1	251	260	PF02991	0.325
LIG_LIR_Nem_3	228	234	PF02991	0.280
LIG_LIR_Nem_3	251	255	PF02991	0.325
LIG_Pex14_1	194	198	PF04695	0.355
LIG_Pex14_2	225	229	PF04695	0.279
LIG_Pex14_2	248	252	PF04695	0.357
LIG_PTAP_UEV_1	60	65	PF05743	0.630
LIG_REV1ctd_RIR_1	25	33	PF16727	0.458
LIG_RPA_C_Fungi	111	123	PF08784	0.487
LIG_SH2_PTP2	231	234	PF00017	0.210
LIG_SH2_SRC	318	321	PF00017	0.306
LIG_SH2_STAT5	231	234	PF00017	0.255
LIG_SH2_STAT5	284	287	PF00017	0.279
LIG_SH3_3	220	226	PF00018	0.310
LIG_SH3_3	58	64	PF00018	0.805
LIG_SH3_3	83	89	PF00018	0.509
LIG_TRAF2_1	94	97	PF00917	0.493
LIG_TRAF2_2	9	14	PF00917	0.537
LIG_UBA3_1	122	129	PF00899	0.384
LIG_WRC_WIRS_1	175	180	PF05994	0.593
MOD_CK1_1	155	161	PF00069	0.666
MOD_CK1_1	174	180	PF00069	0.464
MOD_CK1_1	308	314	PF00069	0.350
MOD_CK1_1	56	62	PF00069	0.718
MOD_CK2_1	115	121	PF00069	0.468
MOD_CK2_1	162	168	PF00069	0.602
MOD_CK2_1	199	205	PF00069	0.329
MOD_GlcNHglycan	158	161	PF01048	0.704
MOD_GlcNHglycan	164	167	PF01048	0.627
MOD_GlcNHglycan	234	237	PF01048	0.410
MOD_GlcNHglycan	281	284	PF01048	0.471
MOD_GlcNHglycan	293	296	PF01048	0.442
MOD_GlcNHglycan	34	37	PF01048	0.398
MOD_GlcNHglycan	340	343	PF01048	0.628
MOD_GlcNHglycan	61	64	PF01048	0.761
MOD_GSK3_1	152	159	PF00069	0.694
MOD_GSK3_1	272	279	PF00069	0.302
MOD_GSK3_1	53	60	PF00069	0.627
MOD_GSK3_1	70	77	PF00069	0.795
MOD_N-GLC_1	153	158	PF02516	0.583
MOD_NEK2_1	100	105	PF00069	0.427
MOD_NEK2_1	152	157	PF00069	0.621
MOD_NEK2_1	162	167	PF00069	0.691
MOD_NEK2_1	291	296	PF00069	0.492
MOD_PIKK_1	70	76	PF00454	0.693
MOD_PKA_1	12	18	PF00069	0.732
MOD_PKA_2	115	121	PF00069	0.483
MOD_PKA_2	12	18	PF00069	0.720
MOD_Plk_4	276	282	PF00069	0.392
MOD_ProDKin_1	218	224	PF00069	0.488
MOD_ProDKin_1	242	248	PF00069	0.441
MOD_ProDKin_1	305	311	PF00069	0.279
MOD_ProDKin_1	53	59	PF00069	0.792
MOD_ProDKin_1	84	90	PF00069	0.616
TRG_ENDOCYTIC_2	231	234	PF00928	0.279
TRG_ER_diArg_1	349	351	PF00400	0.577
TRG_Pf-PMV_PEXEL_1	335	340	PF00026	0.572

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDK0	Leptomonas seymouri	44%	100%
A0A1X0NRN9	Trypanosomatidae	46%	100%
A0A3S7X0Z1	Leishmania donovani	93%	100%
A0A422N807	Trypanosoma rangeli	39%	100%
A4HFZ8	Leishmania braziliensis	72%	99%
A4I317	Leishmania infantum	94%	100%
C9ZJI6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
E9AZC7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
V5BIV4	Trypanosoma cruzi	38%	100%