Putative elongation initiation factor 2 alpha subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

eukaryotic translation initiation factor 2 subunit alpha

Species:

Leishmania major

UniProt:

E9ACP3_LEIMA

TriTrypDb:

LmjF.03.0980 , LMJLV39_030015200 , LMJSD75_030015300

Length:

432

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 16

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 6

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 6

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	2
GO:0005737	cytoplasm	2	2
GO:0005850	eukaryotic translation initiation factor 2 complex	2	2
GO:0005851	eukaryotic translation initiation factor 2B complex	2	2
GO:0032991	protein-containing complex	1	2
GO:0033290	eukaryotic 48S preinitiation complex	4	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0043614	multi-eIF complex	3	2
GO:0070993	translation preinitiation complex	3	2
GO:0110165	cellular anatomical entity	1	2
GO:1990904	ribonucleoprotein complex	2	2

Expansion

Sequence features

E9ACP3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ACP3

Function

Biological processes
Term	Name	Level	Count
GO:0006413	translational initiation	3	2
GO:0006950	response to stress	2	2
GO:0007154	cell communication	2	2
GO:0008152	metabolic process	1	2
GO:0009267	cellular response to starvation	4	2
GO:0009605	response to external stimulus	2	2
GO:0009987	cellular process	1	2
GO:0009991	response to extracellular stimulus	3	2
GO:0010468	regulation of gene expression	5	2
GO:0010608	post-transcriptional regulation of gene expression	6	2
GO:0019222	regulation of metabolic process	3	2
GO:0031667	response to nutrient levels	4	2
GO:0031668	cellular response to extracellular stimulus	3	2
GO:0031669	cellular response to nutrient levels	4	2
GO:0033554	cellular response to stress	3	2
GO:0042594	response to starvation	3	2
GO:0044237	cellular metabolic process	2	2
GO:0050789	regulation of biological process	2	2
GO:0050896	response to stimulus	1	2
GO:0051716	cellular response to stimulus	2	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065007	biological regulation	1	2
GO:0071496	cellular response to external stimulus	3	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003743	translation initiation factor activity	4	11
GO:0005488	binding	1	11
GO:0008135	translation factor activity, RNA binding	3	11
GO:0043021	ribonucleoprotein complex binding	3	2
GO:0043022	ribosome binding	4	2
GO:0044877	protein-containing complex binding	2	2
GO:0045182	translation regulator activity	1	11
GO:0090079	translation regulator activity, nucleic acid binding	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	187	189	PF00675	0.399
CLV_NRD_NRD_1	63	65	PF00675	0.529
CLV_PCSK_KEX2_1	119	121	PF00082	0.510
CLV_PCSK_KEX2_1	174	176	PF00082	0.399
CLV_PCSK_KEX2_1	186	188	PF00082	0.399
CLV_PCSK_KEX2_1	397	399	PF00082	0.551
CLV_PCSK_KEX2_1	63	65	PF00082	0.529
CLV_PCSK_PC1ET2_1	119	121	PF00082	0.510
CLV_PCSK_PC1ET2_1	174	176	PF00082	0.399
CLV_PCSK_PC1ET2_1	397	399	PF00082	0.520
CLV_PCSK_PC7_1	115	121	PF00082	0.600
CLV_PCSK_SKI1_1	11	15	PF00082	0.725
CLV_PCSK_SKI1_1	194	198	PF00082	0.319
CLV_PCSK_SKI1_1	221	225	PF00082	0.459
CLV_PCSK_SKI1_1	317	321	PF00082	0.388
CLV_PCSK_SKI1_1	339	343	PF00082	0.333
CLV_PCSK_SKI1_1	64	68	PF00082	0.529
DEG_Nend_Nbox_1	1	3	PF02207	0.477
DOC_ANK_TNKS_1	345	352	PF00023	0.599
DOC_CKS1_1	222	227	PF01111	0.584
DOC_CYCLIN_RxL_1	311	324	PF00134	0.584
DOC_CYCLIN_RxL_1	61	70	PF00134	0.622
DOC_CYCLIN_yCln2_LP_2	68	74	PF00134	0.548
DOC_MAPK_gen_1	208	218	PF00069	0.502
DOC_MAPK_gen_1	396	404	PF00069	0.520
DOC_MAPK_HePTP_8	76	88	PF00069	0.559
DOC_MAPK_MEF2A_6	148	157	PF00069	0.519
DOC_MAPK_MEF2A_6	311	318	PF00069	0.622
DOC_MAPK_MEF2A_6	79	88	PF00069	0.561
DOC_MAPK_NFAT4_5	311	319	PF00069	0.622
DOC_PP1_RVXF_1	9	15	PF00149	0.480
DOC_PP4_FxxP_1	72	75	PF00568	0.443
DOC_USP7_UBL2_3	119	123	PF12436	0.534
DOC_WW_Pin1_4	221	226	PF00397	0.582
DOC_WW_Pin1_4	25	30	PF00397	0.551
DOC_WW_Pin1_4	403	408	PF00397	0.623
LIG_14-3-3_CanoR_1	187	193	PF00244	0.587
LIG_14-3-3_CanoR_1	41	48	PF00244	0.497
LIG_14-3-3_CanoR_1	56	62	PF00244	0.530
LIG_Actin_WH2_2	295	313	PF00022	0.599
LIG_Actin_WH2_2	388	405	PF00022	0.496
LIG_APCC_ABBA_1	178	183	PF00400	0.519
LIG_APCC_ABBA_1	319	324	PF00400	0.599
LIG_Clathr_ClatBox_1	85	89	PF01394	0.463
LIG_DLG_GKlike_1	188	195	PF00625	0.599
LIG_FAT_LD_1	81	89	PF03623	0.590
LIG_FHA_1	161	167	PF00498	0.509
LIG_FHA_1	284	290	PF00498	0.533
LIG_FHA_1	390	396	PF00498	0.522
LIG_FHA_1	41	47	PF00498	0.484
LIG_FHA_2	130	136	PF00498	0.554
LIG_FHA_2	222	228	PF00498	0.469
LIG_LIR_Apic_2	70	75	PF02991	0.479
LIG_LIR_Gen_1	151	160	PF02991	0.519
LIG_LIR_Gen_1	212	223	PF02991	0.519
LIG_LIR_Gen_1	320	330	PF02991	0.599
LIG_LIR_Nem_3	151	157	PF02991	0.519
LIG_LIR_Nem_3	212	218	PF02991	0.519
LIG_LIR_Nem_3	320	325	PF02991	0.599
LIG_LIR_Nem_3	44	50	PF02991	0.484
LIG_LIR_Nem_3	53	57	PF02991	0.529
LIG_NRBOX	80	86	PF00104	0.527
LIG_Pex14_1	277	281	PF04695	0.571
LIG_RPA_C_Fungi	183	195	PF08784	0.484
LIG_SH2_CRK	48	52	PF00017	0.469
LIG_SH2_NCK_1	181	185	PF00017	0.372
LIG_SH2_PTP2	215	218	PF00017	0.372
LIG_SH2_STAT5	142	145	PF00017	0.547
LIG_SH2_STAT5	181	184	PF00017	0.356
LIG_SH2_STAT5	215	218	PF00017	0.372
LIG_SH2_STAT5	23	26	PF00017	0.580
LIG_SH2_STAT5	54	57	PF00017	0.480
LIG_SH3_3	401	407	PF00018	0.528
LIG_SUMO_SIM_anti_2	340	345	PF11976	0.391
LIG_SUMO_SIM_anti_2	82	89	PF11976	0.522
LIG_SUMO_SIM_par_1	82	89	PF11976	0.453
LIG_TYR_ITIM	179	184	PF00017	0.372
LIG_UBA3_1	292	300	PF00899	0.391
LIG_UBA3_1	306	311	PF00899	0.391
LIG_UBA3_1	84	90	PF00899	0.523
LIG_WW_1	365	368	PF00397	0.372
MOD_CK1_1	19	25	PF00069	0.398
MOD_CK1_1	28	34	PF00069	0.599
MOD_CK2_1	103	109	PF00069	0.697
MOD_CK2_1	221	227	PF00069	0.471
MOD_CK2_1	344	350	PF00069	0.439
MOD_Cter_Amidation	172	175	PF01082	0.484
MOD_Cter_Amidation	416	419	PF01082	0.837
MOD_GlcNHglycan	162	166	PF01048	0.372
MOD_GlcNHglycan	94	97	PF01048	0.573
MOD_GSK3_1	19	26	PF00069	0.580
MOD_N-GLC_1	16	21	PF02516	0.466
MOD_N-GLC_1	160	165	PF02516	0.372
MOD_N-GLC_1	283	288	PF02516	0.391
MOD_N-GLC_1	57	62	PF02516	0.571
MOD_NEK2_1	1	6	PF00069	0.604
MOD_NEK2_1	129	134	PF00069	0.506
MOD_NEK2_1	306	311	PF00069	0.410
MOD_NEK2_1	344	349	PF00069	0.439
MOD_NEK2_1	57	62	PF00069	0.574
MOD_PIKK_1	155	161	PF00454	0.484
MOD_PKA_2	40	46	PF00069	0.500
MOD_PKB_1	186	194	PF00069	0.484
MOD_Plk_1	16	22	PF00069	0.452
MOD_Plk_1	161	167	PF00069	0.372
MOD_Plk_1	325	331	PF00069	0.445
MOD_Plk_1	57	63	PF00069	0.576
MOD_Plk_2-3	77	83	PF00069	0.534
MOD_Plk_4	317	323	PF00069	0.521
MOD_Plk_4	89	95	PF00069	0.532
MOD_ProDKin_1	221	227	PF00069	0.582
MOD_ProDKin_1	25	31	PF00069	0.553
MOD_ProDKin_1	403	409	PF00069	0.626
MOD_SUMO_for_1	296	299	PF00179	0.283
MOD_SUMO_for_1	376	379	PF00179	0.562
MOD_SUMO_rev_2	100	106	PF00179	0.706
MOD_SUMO_rev_2	133	139	PF00179	0.543
MOD_SUMO_rev_2	27	36	PF00179	0.543
TRG_ENDOCYTIC_2	181	184	PF00928	0.372
TRG_ENDOCYTIC_2	215	218	PF00928	0.372
TRG_ENDOCYTIC_2	47	50	PF00928	0.472
TRG_ENDOCYTIC_2	54	57	PF00928	0.505
TRG_ER_diArg_1	186	188	PF00400	0.372
TRG_ER_diArg_1	370	373	PF00400	0.388
TRG_NES_CRM1_1	144	159	PF08389	0.484

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P537	Leptomonas seymouri	86%	100%
A0A1X0P943	Trypanosomatidae	62%	100%
A0A3S7WNN3	Leishmania donovani	99%	100%
A0A422NET9	Trypanosoma rangeli	60%	100%
A4H3R0	Leishmania braziliensis	95%	100%
A4HRY4	Leishmania infantum	99%	100%
C9ZKJ3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	100%
E9AJY2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	99%	100%
P05198	Homo sapiens	31%	100%
P68101	Rattus norvegicus	31%	100%
P68102	Bos taurus	31%	100%
Q5R493	Pongo abelii	31%	100%
Q5ZLX2	Gallus gallus	31%	100%
Q6GL89	Xenopus tropicalis	32%	100%
Q6ZWX6	Mus musculus	31%	100%
Q7ZTK4	Xenopus laevis	33%	100%
Q869N9	Dictyostelium discoideum	30%	100%
Q9SIZ2	Arabidopsis thaliana	33%	100%
V5BSN3	Trypanosoma cruzi	60%	100%