Ammonia transport outward protein 2

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GPR1/FUN34/yaaH family, putative

Species:

Leishmania major

UniProt:

E9ACI2_LEIMA

TriTrypDb:

LmjF.03.0370 , LMJLV39_030008800 , LMJSD75_030008900

Length:

310

Annotations

LeishMANIAdb annotations

Most closely related to Prokaryotic SatP transporters. Especially expanded in the Leptomonas lineage

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 19
NetGPI	no	yes: 0, no: 19

Cellular components
Term	Name	Level	Count
GO:0005886	plasma membrane	3	4
GO:0016020	membrane	2	20
GO:0110165	cellular anatomical entity	1	20

Expansion

Sequence features

E9ACI2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ACI2

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	4
GO:0006811	monoatomic ion transport	4	4
GO:0006820	monoatomic anion transport	5	4
GO:0006835	dicarboxylic acid transport	7	4
GO:0006846	acetate transport	6	4
GO:0009987	cellular process	1	4
GO:0015711	organic anion transport	5	4
GO:0015718	monocarboxylic acid transport	7	4
GO:0015740	C4-dicarboxylate transport	8	4
GO:0015744	succinate transport	6	4
GO:0015849	organic acid transport	5	4
GO:0034220	monoatomic ion transmembrane transport	3	4
GO:0035433	acetate transmembrane transport	5	4
GO:0046942	carboxylic acid transport	6	4
GO:0051179	localization	1	4
GO:0051234	establishment of localization	2	4
GO:0055085	transmembrane transport	2	4
GO:0071422	succinate transmembrane transport	5	4
GO:0071702	organic substance transport	4	4
GO:0098656	monoatomic anion transmembrane transport	4	4
GO:1903825	organic acid transmembrane transport	3	4
GO:1905039	carboxylic acid transmembrane transport	4	4

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	4
GO:0005342	organic acid transmembrane transporter activity	3	4
GO:0008028	monocarboxylic acid transmembrane transporter activity	5	4
GO:0008324	monoatomic cation transmembrane transporter activity	4	4
GO:0008509	monoatomic anion transmembrane transporter activity	4	4
GO:0008514	organic anion transmembrane transporter activity	5	4
GO:0015075	monoatomic ion transmembrane transporter activity	3	4
GO:0015078	proton transmembrane transporter activity	5	4
GO:0015123	acetate transmembrane transporter activity	6	4
GO:0015291	secondary active transmembrane transporter activity	4	4
GO:0015293	symporter activity	5	4
GO:0015294	solute:monoatomic cation symporter activity	5	4
GO:0015295	solute:proton symporter activity	6	4
GO:0015318	inorganic molecular entity transmembrane transporter activity	3	4
GO:0015355	secondary active monocarboxylate transmembrane transporter activity	5	4
GO:0015360	acetate:proton symporter activity	7	4
GO:0022804	active transmembrane transporter activity	3	4
GO:0022853	active monoatomic ion transmembrane transporter activity	4	4
GO:0022857	transmembrane transporter activity	2	4
GO:0022890	inorganic cation transmembrane transporter activity	4	4
GO:0043893	acetate:monoatomic cation symporter activity	6	4
GO:0046943	carboxylic acid transmembrane transporter activity	4	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	207	211	PF00656	0.210
CLV_NRD_NRD_1	106	108	PF00675	0.483
CLV_NRD_NRD_1	43	45	PF00675	0.535
CLV_NRD_NRD_1	48	50	PF00675	0.512
CLV_NRD_NRD_1	86	88	PF00675	0.506
CLV_PCSK_KEX2_1	106	108	PF00082	0.407
CLV_PCSK_KEX2_1	112	114	PF00082	0.396
CLV_PCSK_KEX2_1	303	305	PF00082	0.447
CLV_PCSK_KEX2_1	42	44	PF00082	0.518
CLV_PCSK_KEX2_1	86	88	PF00082	0.519
CLV_PCSK_PC1ET2_1	112	114	PF00082	0.372
CLV_PCSK_PC1ET2_1	303	305	PF00082	0.513
CLV_PCSK_SKI1_1	294	298	PF00082	0.386
DEG_APCC_DBOX_1	293	301	PF00400	0.639
DOC_MAPK_DCC_7	120	129	PF00069	0.498
DOC_MAPK_gen_1	42	53	PF00069	0.709
DOC_MAPK_MEF2A_6	294	301	PF00069	0.638
DOC_PP4_FxxP_1	198	201	PF00568	0.376
DOC_PP4_FxxP_1	233	236	PF00568	0.517
DOC_PP4_FxxP_1	244	247	PF00568	0.168
DOC_USP7_MATH_1	201	205	PF00917	0.286
DOC_USP7_MATH_1	27	31	PF00917	0.763
DOC_USP7_UBL2_3	32	36	PF12436	0.629
DOC_WW_Pin1_4	112	117	PF00397	0.714
DOC_WW_Pin1_4	120	125	PF00397	0.485
DOC_WW_Pin1_4	232	237	PF00397	0.519
DOC_WW_Pin1_4	90	95	PF00397	0.630
LIG_14-3-3_CanoR_1	176	180	PF00244	0.488
LIG_14-3-3_CanoR_1	234	244	PF00244	0.429
LIG_Actin_WH2_2	275	291	PF00022	0.376
LIG_APCC_ABBA_1	5	10	PF00400	0.637
LIG_APCC_ABBAyCdc20_2	4	10	PF00400	0.606
LIG_BRCT_BRCA1_1	225	229	PF00533	0.440
LIG_BRCT_BRCA1_1	253	257	PF00533	0.254
LIG_EH1_1	131	139	PF00400	0.270
LIG_FHA_1	133	139	PF00498	0.331
LIG_FHA_1	151	157	PF00498	0.336
LIG_FHA_1	162	168	PF00498	0.279
LIG_FHA_1	192	198	PF00498	0.317
LIG_FHA_1	36	42	PF00498	0.665
LIG_FHA_2	285	291	PF00498	0.372
LIG_Integrin_RGD_1	208	210	PF01839	0.391
LIG_LIR_Apic_2	231	236	PF02991	0.429
LIG_LIR_Gen_1	235	245	PF02991	0.514
LIG_LIR_Nem_3	184	190	PF02991	0.330
LIG_Pex14_2	166	170	PF04695	0.362
LIG_Pex14_2	217	221	PF04695	0.325
LIG_Pex14_2	229	233	PF04695	0.341
LIG_SH2_STAT5	205	208	PF00017	0.281
LIG_SH2_STAT5	270	273	PF00017	0.369
LIG_SH2_STAT5	281	284	PF00017	0.322
LIG_SH2_STAT5	307	310	PF00017	0.621
LIG_SH2_STAT5	55	58	PF00017	0.713
LIG_SH3_1	113	119	PF00018	0.391
LIG_SH3_3	10	16	PF00018	0.645
LIG_SH3_3	113	119	PF00018	0.500
LIG_UBA3_1	296	303	PF00899	0.551
LIG_Vh1_VBS_1	264	282	PF01044	0.201
MOD_CK1_1	232	238	PF00069	0.429
MOD_CK1_1	30	36	PF00069	0.838
MOD_CK1_1	74	80	PF00069	0.747
MOD_CK2_1	201	207	PF00069	0.296
MOD_CK2_1	55	61	PF00069	0.747
MOD_CK2_1	74	80	PF00069	0.740
MOD_GSK3_1	175	182	PF00069	0.482
MOD_GSK3_1	18	25	PF00069	0.681
MOD_GSK3_1	224	231	PF00069	0.436
MOD_N-GLC_1	71	76	PF02516	0.468
MOD_N-GLC_2	145	147	PF02516	0.420
MOD_NEK2_1	132	137	PF00069	0.328
MOD_NEK2_1	179	184	PF00069	0.316
MOD_NEK2_1	224	229	PF00069	0.320
MOD_NEK2_1	251	256	PF00069	0.361
MOD_NEK2_1	258	263	PF00069	0.304
MOD_NEK2_1	284	289	PF00069	0.376
MOD_PIKK_1	161	167	PF00454	0.276
MOD_PIKK_1	74	80	PF00454	0.818
MOD_PKA_2	175	181	PF00069	0.429
MOD_Plk_1	201	207	PF00069	0.245
MOD_Plk_1	71	77	PF00069	0.749
MOD_Plk_1	80	86	PF00069	0.740
MOD_Plk_4	133	139	PF00069	0.318
MOD_Plk_4	201	207	PF00069	0.345
MOD_Plk_4	224	230	PF00069	0.326
MOD_Plk_4	277	283	PF00069	0.376
MOD_ProDKin_1	112	118	PF00069	0.711
MOD_ProDKin_1	120	126	PF00069	0.485
MOD_ProDKin_1	232	238	PF00069	0.519
MOD_ProDKin_1	90	96	PF00069	0.630
TRG_DiLeu_BaEn_4	80	86	PF01217	0.645
TRG_ENDOCYTIC_2	187	190	PF00928	0.355
TRG_ENDOCYTIC_2	270	273	PF00928	0.365
TRG_ENDOCYTIC_2	281	284	PF00928	0.333
TRG_ER_diArg_1	304	307	PF00400	0.625
TRG_ER_diArg_1	41	44	PF00400	0.807
TRG_ER_diArg_1	85	87	PF00400	0.701

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I0C8	Leptomonas seymouri	58%	97%
A0A0N1PAF6	Leptomonas seymouri	28%	85%
A0A1X0P9Q6	Trypanosomatidae	30%	100%
A0A3R7M294	Trypanosoma rangeli	36%	100%
A0A3R7MT82	Trypanosoma rangeli	30%	100%
A0A3S5H538	Leishmania donovani	27%	84%
A0A3S5H540	Leishmania donovani	27%	100%
A0A451EJN9	Leishmania donovani	87%	100%
A4H3K7	Leishmania braziliensis	26%	87%
A4HRU7	Leishmania infantum	88%	100%
A4HRU8	Leishmania infantum	27%	84%
A4HRV0	Leishmania infantum	27%	100%
E9ACI3	Leishmania major	28%	84%
E9ACI5	Leishmania major	25%	100%
E9AJS1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	78%	100%
E9AJS2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	83%
E9AJS3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
V5DU51	Trypanosoma cruzi	36%	95%