Putative U2 splicing auxiliary factor

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

U2 splicing auxiliary factor, putative

Species:

Leishmania major

UniProt:

E9ACG6_LEIMA

TriTrypDb:

LmjF.03.0190 , LMJLV39_030006900 , LMJSD75_030006900

Length:

210

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	2
GO:0005681	spliceosomal complex	3	2
GO:0032991	protein-containing complex	1	12
GO:0089701	U2AF complex	3	12
GO:0110165	cellular anatomical entity	1	2
GO:0140513	nuclear protein-containing complex	2	12
GO:1990904	ribonucleoprotein complex	2	2

Expansion

Sequence features

E9ACG6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ACG6

Function

Biological processes
Term	Name	Level	Count
GO:0000375	RNA splicing, via transesterification reactions	8	12
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	12
GO:0000398	mRNA splicing, via spliceosome	8	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006397	mRNA processing	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0008380	RNA splicing	7	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0005488	binding	1	12
GO:0030628	pre-mRNA 3'-splice site binding	6	2
GO:0036002	pre-mRNA binding	5	2
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0008270	zinc ion binding	6	6
GO:0046914	transition metal ion binding	5	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	169	171	PF00675	0.407
CLV_NRD_NRD_1	188	190	PF00675	0.394
CLV_NRD_NRD_1	196	198	PF00675	0.588
CLV_PCSK_KEX2_1	169	171	PF00082	0.543
CLV_PCSK_KEX2_1	188	190	PF00082	0.340
CLV_PCSK_KEX2_1	198	200	PF00082	0.546
CLV_PCSK_PC1ET2_1	198	200	PF00082	0.570
CLV_PCSK_SKI1_1	115	119	PF00082	0.258
CLV_PCSK_SKI1_1	169	173	PF00082	0.465
CLV_PCSK_SKI1_1	63	67	PF00082	0.388
CLV_PCSK_SKI1_1	7	11	PF00082	0.292
DEG_APCC_DBOX_1	168	176	PF00400	0.496
DOC_CDC14_PxL_1	128	136	PF14671	0.319
DOC_CYCLIN_RxL_1	163	176	PF00134	0.488
DOC_MAPK_gen_1	121	131	PF00069	0.439
DOC_MAPK_HePTP_8	26	38	PF00069	0.378
DOC_MAPK_MEF2A_6	29	38	PF00069	0.368
DOC_PP1_RVXF_1	5	12	PF00149	0.297
DOC_PP4_FxxP_1	107	110	PF00568	0.480
DOC_USP7_UBL2_3	177	181	PF12436	0.438
DOC_USP7_UBL2_3	62	66	PF12436	0.440
DOC_WW_Pin1_4	132	137	PF00397	0.333
DOC_WW_Pin1_4	201	206	PF00397	0.556
DOC_WW_Pin1_4	30	35	PF00397	0.354
LIG_deltaCOP1_diTrp_1	53	58	PF00928	0.453
LIG_deltaCOP1_diTrp_1	75	83	PF00928	0.451
LIG_FHA_1	112	118	PF00498	0.489
LIG_FHA_2	77	83	PF00498	0.451
LIG_GBD_Chelix_1	112	120	PF00786	0.325
LIG_GBD_Chelix_1	91	99	PF00786	0.239
LIG_HCF-1_HBM_1	70	73	PF13415	0.365
LIG_LIR_Gen_1	79	87	PF02991	0.451
LIG_LIR_Nem_3	70	76	PF02991	0.342
LIG_LIR_Nem_3	79	83	PF02991	0.427
LIG_MYND_1	132	136	PF01753	0.336
LIG_REV1ctd_RIR_1	8	17	PF16727	0.292
LIG_SH2_SRC	161	164	PF00017	0.340
LIG_SH2_STAT5	104	107	PF00017	0.430
LIG_SH2_STAT5	161	164	PF00017	0.326
LIG_SH2_STAT5	64	67	PF00017	0.367
LIG_SH3_3	33	39	PF00018	0.334
LIG_SUMO_SIM_anti_2	127	133	PF11976	0.372
LIG_SUMO_SIM_par_1	127	133	PF11976	0.367
LIG_UBA3_1	171	177	PF00899	0.404
MOD_CK1_1	135	141	PF00069	0.375
MOD_CK2_1	76	82	PF00069	0.451
MOD_Cter_Amidation	121	124	PF01082	0.344
MOD_DYRK1A_RPxSP_1	201	205	PF00069	0.576
MOD_GSK3_1	197	204	PF00069	0.617
MOD_LATS_1	27	33	PF00433	0.464
MOD_N-GLC_2	151	153	PF02516	0.465
MOD_NEK2_1	165	170	PF00069	0.442
MOD_PKA_1	197	203	PF00069	0.689
MOD_PKB_1	197	205	PF00069	0.618
MOD_Plk_4	32	38	PF00069	0.337
MOD_ProDKin_1	132	138	PF00069	0.343
MOD_ProDKin_1	201	207	PF00069	0.557
MOD_ProDKin_1	30	36	PF00069	0.348
MOD_SUMO_rev_2	191	200	PF00179	0.668
MOD_SUMO_rev_2	55	65	PF00179	0.425
TRG_DiLeu_BaLyEn_6	167	172	PF01217	0.523
TRG_ENDOCYTIC_2	104	107	PF00928	0.439
TRG_ENDOCYTIC_2	161	164	PF00928	0.340
TRG_ENDOCYTIC_2	73	76	PF00928	0.349
TRG_ER_diArg_1	169	171	PF00400	0.534
TRG_NLS_Bipartite_1	184	202	PF00514	0.618
TRG_NLS_MonoExtN_4	197	202	PF00514	0.672
TRG_Pf-PMV_PEXEL_1	169	173	PF00026	0.408

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJ76	Leptomonas seymouri	88%	81%
A0A0S4JRQ0	Bodo saltans	65%	87%
A0A1X0PA27	Trypanosomatidae	72%	90%
A0A3R7M283	Trypanosoma rangeli	69%	90%
A0A3S5H527	Leishmania donovani	98%	100%
A1A4K8	Bos taurus	38%	89%
A4H3J1	Leishmania braziliensis	94%	100%
A4HRT0	Leishmania infantum	98%	100%
D0A226	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	81%	85%
E9AJQ4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
Q01081	Homo sapiens	38%	88%
Q09176	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	39%	97%
Q3T127	Bos taurus	40%	95%
Q6AUG0	Oryza sativa subsp. japonica	39%	69%
Q7TP17	Rattus norvegicus	40%	95%
Q8BGJ9	Mus musculus	40%	95%
Q8WU68	Homo sapiens	38%	95%
Q94535	Drosophila melanogaster	37%	80%
Q9D883	Mus musculus	38%	88%
Q9FMY5	Arabidopsis thaliana	40%	74%
Q9S709	Arabidopsis thaliana	40%	71%
Q9ZQW8	Oryza sativa subsp. japonica	37%	72%
V5D4H1	Trypanosoma cruzi	75%	90%