DNA primase large subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA primase large subunit, putative

Species:

Leishmania major

UniProt:

E9ACF6_LEIMA

TriTrypDb:

LmjF.03.0090 , LMJLV39_030005900 , LMJSD75_030005900

Length:

567

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	12
GO:0005658	alpha DNA polymerase:primase complex	4	12
GO:0005739	mitochondrion	5	2
GO:0030880	RNA polymerase complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0042575	DNA polymerase complex	3	12
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	12
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	12
GO:0110165	cellular anatomical entity	1	2
GO:0140513	nuclear protein-containing complex	2	12
GO:0140535	intracellular protein-containing complex	2	12
GO:1902494	catalytic complex	2	12
GO:1990234	transferase complex	3	12

Expansion

Sequence features

E9ACF6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ACF6

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006260	DNA replication	5	2
GO:0006261	DNA-templated DNA replication	6	2
GO:0006269	DNA replication, synthesis of RNA primer	6	12
GO:0006270	DNA replication initiation	5	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009059	macromolecule biosynthetic process	4	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0018130	heterocycle biosynthetic process	4	12
GO:0019438	aromatic compound biosynthetic process	4	12
GO:0022402	cell cycle process	2	2
GO:0032774	RNA biosynthetic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034654	nucleobase-containing compound biosynthetic process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0044271	cellular nitrogen compound biosynthetic process	4	12
GO:0044786	cell cycle DNA replication	3	2
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901362	organic cyclic compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003677	DNA binding	4	12
GO:0003824	catalytic activity	1	3
GO:0003896	DNA primase activity	7	2
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	2
GO:0005488	binding	1	12
GO:0016740	transferase activity	2	3
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	3
GO:0016779	nucleotidyltransferase activity	4	3
GO:0034062	5'-3' RNA polymerase activity	5	2
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0051536	iron-sulfur cluster binding	3	12
GO:0051539	4 iron, 4 sulfur cluster binding	4	12
GO:0051540	metal cluster binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097747	RNA polymerase activity	4	2
GO:0140098	catalytic activity, acting on RNA	3	2
GO:0140640	catalytic activity, acting on a nucleic acid	2	2
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	76	80	PF00656	0.505
CLV_NRD_NRD_1	118	120	PF00675	0.689
CLV_NRD_NRD_1	221	223	PF00675	0.529
CLV_NRD_NRD_1	359	361	PF00675	0.350
CLV_NRD_NRD_1	36	38	PF00675	0.373
CLV_NRD_NRD_1	514	516	PF00675	0.368
CLV_NRD_NRD_1	89	91	PF00675	0.530
CLV_PCSK_KEX2_1	118	120	PF00082	0.689
CLV_PCSK_KEX2_1	184	186	PF00082	0.537
CLV_PCSK_KEX2_1	195	197	PF00082	0.456
CLV_PCSK_KEX2_1	221	223	PF00082	0.529
CLV_PCSK_KEX2_1	252	254	PF00082	0.422
CLV_PCSK_KEX2_1	359	361	PF00082	0.350
CLV_PCSK_KEX2_1	514	516	PF00082	0.308
CLV_PCSK_KEX2_1	54	56	PF00082	0.369
CLV_PCSK_KEX2_1	550	552	PF00082	0.627
CLV_PCSK_KEX2_1	89	91	PF00082	0.530
CLV_PCSK_PC1ET2_1	184	186	PF00082	0.537
CLV_PCSK_PC1ET2_1	195	197	PF00082	0.456
CLV_PCSK_PC1ET2_1	252	254	PF00082	0.424
CLV_PCSK_PC1ET2_1	54	56	PF00082	0.369
CLV_PCSK_PC1ET2_1	550	552	PF00082	0.606
CLV_PCSK_PC7_1	248	254	PF00082	0.424
CLV_PCSK_SKI1_1	153	157	PF00082	0.346
CLV_PCSK_SKI1_1	171	175	PF00082	0.288
CLV_PCSK_SKI1_1	185	189	PF00082	0.522
CLV_PCSK_SKI1_1	221	225	PF00082	0.580
CLV_PCSK_SKI1_1	248	252	PF00082	0.307
CLV_PCSK_SKI1_1	253	257	PF00082	0.298
CLV_PCSK_SKI1_1	461	465	PF00082	0.303
DEG_Nend_UBRbox_3	1	3	PF02207	0.620
DOC_CKS1_1	532	537	PF01111	0.662
DOC_CYCLIN_RxL_1	168	175	PF00134	0.450
DOC_MAPK_gen_1	550	556	PF00069	0.703
DOC_PP1_RVXF_1	350	357	PF00149	0.334
DOC_PP2B_LxvP_1	436	439	PF13499	0.338
DOC_PP4_FxxP_1	519	522	PF00568	0.303
DOC_PP4_FxxP_1	532	535	PF00568	0.570
DOC_USP7_MATH_1	228	232	PF00917	0.587
DOC_USP7_MATH_1	542	546	PF00917	0.688
DOC_USP7_MATH_2	522	528	PF00917	0.424
DOC_USP7_MATH_2	86	92	PF00917	0.607
DOC_WW_Pin1_4	479	484	PF00397	0.443
DOC_WW_Pin1_4	5	10	PF00397	0.649
DOC_WW_Pin1_4	518	523	PF00397	0.303
DOC_WW_Pin1_4	531	536	PF00397	0.620
DOC_WW_Pin1_4	65	70	PF00397	0.484
LIG_14-3-3_CanoR_1	171	180	PF00244	0.445
LIG_14-3-3_CanoR_1	185	190	PF00244	0.408
LIG_14-3-3_CanoR_1	551	555	PF00244	0.709
LIG_14-3-3_CanoR_1	562	567	PF00244	0.638
LIG_BRCT_BRCA1_1	136	140	PF00533	0.562
LIG_BRCT_BRCA1_1	42	46	PF00533	0.554
LIG_BRCT_BRCA1_1	528	532	PF00533	0.552
LIG_Clathr_ClatBox_1	45	49	PF01394	0.450
LIG_CtBP_PxDLS_1	242	246	PF00389	0.424
LIG_deltaCOP1_diTrp_1	160	165	PF00928	0.387
LIG_FHA_1	145	151	PF00498	0.453
LIG_FHA_1	167	173	PF00498	0.439
LIG_FHA_1	186	192	PF00498	0.351
LIG_FHA_1	245	251	PF00498	0.297
LIG_FHA_1	550	556	PF00498	0.598
LIG_FHA_2	25	31	PF00498	0.591
LIG_FHA_2	323	329	PF00498	0.419
LIG_FHA_2	42	48	PF00498	0.427
LIG_FHA_2	480	486	PF00498	0.370
LIG_FHA_2	532	538	PF00498	0.670
LIG_LIR_Apic_2	236	242	PF02991	0.338
LIG_LIR_Apic_2	516	522	PF02991	0.303
LIG_LIR_Apic_2	529	535	PF02991	0.440
LIG_LIR_Gen_1	271	281	PF02991	0.303
LIG_LIR_Gen_1	28	39	PF02991	0.407
LIG_LIR_Gen_1	388	396	PF02991	0.338
LIG_LIR_Nem_3	216	220	PF02991	0.456
LIG_LIR_Nem_3	236	241	PF02991	0.120
LIG_LIR_Nem_3	271	276	PF02991	0.319
LIG_LIR_Nem_3	388	393	PF02991	0.421
LIG_LIR_Nem_3	475	479	PF02991	0.326
LIG_LIR_Nem_3	521	526	PF02991	0.309
LIG_LYPXL_yS_3	476	479	PF13949	0.388
LIG_PCNA_PIPBox_1	308	317	PF02747	0.303
LIG_Pex14_1	31	35	PF04695	0.382
LIG_Pex14_2	273	277	PF04695	0.319
LIG_Pex14_2	314	318	PF04695	0.303
LIG_RPA_C_Fungi	217	229	PF08784	0.565
LIG_SH2_CRK	220	224	PF00017	0.596
LIG_SH2_CRK	239	243	PF00017	0.207
LIG_SH2_NCK_1	427	431	PF00017	0.388
LIG_SH2_STAP1	107	111	PF00017	0.697
LIG_SH2_STAP1	526	530	PF00017	0.446
LIG_SH2_STAT5	217	220	PF00017	0.407
LIG_SH2_STAT5	254	257	PF00017	0.422
LIG_SH2_STAT5	289	292	PF00017	0.338
LIG_SH2_STAT5	498	501	PF00017	0.292
LIG_SH2_STAT5	505	508	PF00017	0.287
LIG_SH3_3	136	142	PF00018	0.575
LIG_SH3_3	197	203	PF00018	0.404
LIG_SUMO_SIM_par_1	241	247	PF11976	0.362
LIG_TRAF2_1	141	144	PF00917	0.559
LIG_TRAF2_1	297	300	PF00917	0.424
LIG_TRAF2_1	325	328	PF00917	0.338
LIG_TYR_ITIM	218	223	PF00017	0.532
LIG_UBA3_1	389	397	PF00899	0.305
MOD_CDK_SPxxK_3	65	72	PF00069	0.490
MOD_CK1_1	122	128	PF00069	0.664
MOD_CK1_1	244	250	PF00069	0.407
MOD_CK1_1	426	432	PF00069	0.306
MOD_CK1_1	536	542	PF00069	0.662
MOD_CK1_1	7	13	PF00069	0.626
MOD_CK1_1	92	98	PF00069	0.642
MOD_CK2_1	224	230	PF00069	0.627
MOD_CK2_1	30	36	PF00069	0.506
MOD_CK2_1	322	328	PF00069	0.378
MOD_CK2_1	41	47	PF00069	0.471
MOD_CK2_1	518	524	PF00069	0.303
MOD_CMANNOS	162	165	PF00535	0.369
MOD_Cter_Amidation	421	424	PF01082	0.303
MOD_Cter_Amidation	548	551	PF01082	0.753
MOD_GlcNHglycan	127	130	PF01048	0.630
MOD_GlcNHglycan	226	229	PF01048	0.608
MOD_GlcNHglycan	27	30	PF01048	0.493
MOD_GlcNHglycan	428	431	PF01048	0.388
MOD_GlcNHglycan	540	543	PF01048	0.710
MOD_GlcNHglycan	544	547	PF01048	0.686
MOD_GlcNHglycan	91	94	PF01048	0.581
MOD_GSK3_1	113	120	PF00069	0.671
MOD_GSK3_1	224	231	PF00069	0.578
MOD_GSK3_1	299	306	PF00069	0.424
MOD_GSK3_1	375	382	PF00069	0.309
MOD_GSK3_1	475	482	PF00069	0.400
MOD_GSK3_1	514	521	PF00069	0.302
MOD_GSK3_1	538	545	PF00069	0.629
MOD_GSK3_1	7	14	PF00069	0.570
MOD_GSK3_1	88	95	PF00069	0.642
MOD_N-GLC_1	41	46	PF02516	0.527
MOD_NEK2_1	23	28	PF00069	0.582
MOD_NEK2_1	375	380	PF00069	0.339
MOD_NEK2_1	389	394	PF00069	0.262
MOD_NEK2_1	405	410	PF00069	0.303
MOD_NEK2_1	425	430	PF00069	0.120
MOD_NEK2_1	506	511	PF00069	0.280
MOD_NEK2_2	41	46	PF00069	0.478
MOD_PIKK_1	7	13	PF00454	0.704
MOD_PK_1	550	556	PF00069	0.676
MOD_PKA_1	118	124	PF00069	0.713
MOD_PKA_1	423	429	PF00069	0.338
MOD_PKA_1	514	520	PF00069	0.319
MOD_PKA_1	550	556	PF00069	0.716
MOD_PKA_1	89	95	PF00069	0.589
MOD_PKA_2	117	123	PF00069	0.663
MOD_PKA_2	228	234	PF00069	0.551
MOD_PKA_2	514	520	PF00069	0.302
MOD_PKA_2	542	548	PF00069	0.740
MOD_PKA_2	550	556	PF00069	0.703
MOD_PKA_2	88	94	PF00069	0.639
MOD_Plk_1	383	389	PF00069	0.343
MOD_Plk_1	41	47	PF00069	0.546
MOD_Plk_2-3	30	36	PF00069	0.510
MOD_Plk_4	134	140	PF00069	0.590
MOD_Plk_4	272	278	PF00069	0.265
MOD_Plk_4	30	36	PF00069	0.405
MOD_Plk_4	303	309	PF00069	0.303
MOD_Plk_4	389	395	PF00069	0.314
MOD_Plk_4	41	47	PF00069	0.464
MOD_Plk_4	431	437	PF00069	0.303
MOD_Plk_4	514	520	PF00069	0.303
MOD_Plk_4	550	556	PF00069	0.728
MOD_Plk_4	73	79	PF00069	0.523
MOD_ProDKin_1	479	485	PF00069	0.443
MOD_ProDKin_1	5	11	PF00069	0.650
MOD_ProDKin_1	518	524	PF00069	0.303
MOD_ProDKin_1	531	537	PF00069	0.637
MOD_ProDKin_1	65	71	PF00069	0.483
MOD_SUMO_for_1	178	181	PF00179	0.400
MOD_SUMO_for_1	223	226	PF00179	0.533
MOD_SUMO_rev_2	30	39	PF00179	0.468
MOD_SUMO_rev_2	487	495	PF00179	0.450
TRG_DiLeu_BaLyEn_6	245	250	PF01217	0.303
TRG_ENDOCYTIC_2	220	223	PF00928	0.502
TRG_ENDOCYTIC_2	238	241	PF00928	0.168
TRG_ENDOCYTIC_2	372	375	PF00928	0.298
TRG_ENDOCYTIC_2	476	479	PF00928	0.400
TRG_ENDOCYTIC_2	503	506	PF00928	0.303
TRG_ENDOCYTIC_2	525	528	PF00928	0.397
TRG_ER_diArg_1	220	222	PF00400	0.507
TRG_ER_diArg_1	334	337	PF00400	0.273
TRG_ER_diArg_1	358	360	PF00400	0.348
TRG_NLS_MonoExtN_4	248	255	PF00514	0.424

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDE0	Leptomonas seymouri	79%	98%
A0A0S4JD25	Bodo saltans	50%	100%
A0A1X0P8Q6	Trypanosomatidae	57%	96%
A0A3R7RF31	Trypanosoma rangeli	56%	99%
A0A3S7WNM4	Leishmania donovani	96%	100%
A4H3I1	Leishmania braziliensis	88%	100%
D0A216	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	98%
E9AG28	Leishmania infantum	96%	100%
E9AJP4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
O74761	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	100%
P20457	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
Q55BM5	Dictyostelium discoideum	25%	100%
Q84WJ2	Arabidopsis thaliana	29%	100%
Q8NIZ4	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	27%	100%
Q9VPH2	Drosophila melanogaster	27%	100%
V5B8P2	Trypanosoma cruzi	57%	98%