tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Met-10+ like-protein, putative

Species:

Leishmania major

UniProt:

E9ACF3_LEIMA

TriTrypDb:

LmjF.03.0060 , LMJLV39_030005600 , LMJSD75_030005600

Length:

390

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

E9ACF3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ACF3

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	2
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006400	tRNA modification	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0030488	tRNA methylation	5	2
GO:0032259	methylation	2	4
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008168	methyltransferase activity	4	4
GO:0008173	RNA methyltransferase activity	4	2
GO:0008175	tRNA methyltransferase activity	5	2
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	4
GO:0016765	transferase activity, transferring alkyl or aryl (other than methyl) groups	3	12
GO:0102522	tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity	4	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	134	136	PF00675	0.381
CLV_NRD_NRD_1	156	158	PF00675	0.431
CLV_NRD_NRD_1	161	163	PF00675	0.396
CLV_NRD_NRD_1	166	168	PF00675	0.332
CLV_NRD_NRD_1	22	24	PF00675	0.485
CLV_NRD_NRD_1	44	46	PF00675	0.505
CLV_NRD_NRD_1	76	78	PF00675	0.351
CLV_NRD_NRD_1	80	82	PF00675	0.343
CLV_PCSK_KEX2_1	134	136	PF00082	0.405
CLV_PCSK_KEX2_1	156	158	PF00082	0.421
CLV_PCSK_KEX2_1	161	163	PF00082	0.352
CLV_PCSK_KEX2_1	168	170	PF00082	0.259
CLV_PCSK_KEX2_1	323	325	PF00082	0.317
CLV_PCSK_KEX2_1	37	39	PF00082	0.563
CLV_PCSK_KEX2_1	379	381	PF00082	0.367
CLV_PCSK_KEX2_1	76	78	PF00082	0.332
CLV_PCSK_PC1ET2_1	168	170	PF00082	0.454
CLV_PCSK_PC1ET2_1	323	325	PF00082	0.362
CLV_PCSK_PC1ET2_1	37	39	PF00082	0.565
CLV_PCSK_PC1ET2_1	379	381	PF00082	0.367
CLV_PCSK_PC7_1	157	163	PF00082	0.434
CLV_PCSK_SKI1_1	209	213	PF00082	0.287
CLV_PCSK_SKI1_1	312	316	PF00082	0.362
CLV_PCSK_SKI1_1	34	38	PF00082	0.596
CLV_PCSK_SKI1_1	371	375	PF00082	0.315
CLV_PCSK_SKI1_1	46	50	PF00082	0.558
CLV_PCSK_SKI1_1	7	11	PF00082	0.428
CLV_PCSK_SKI1_1	76	80	PF00082	0.424
DEG_APCC_DBOX_1	134	142	PF00400	0.606
DOC_CKS1_1	26	31	PF01111	0.588
DOC_CYCLIN_RxL_1	4	13	PF00134	0.298
DOC_MAPK_gen_1	134	142	PF00069	0.625
DOC_MAPK_MEF2A_6	134	142	PF00069	0.625
DOC_MAPK_MEF2A_6	7	14	PF00069	0.317
DOC_PIKK_1	95	103	PF02985	0.551
DOC_PP1_RVXF_1	207	213	PF00149	0.470
DOC_PP1_RVXF_1	218	224	PF00149	0.470
DOC_PP4_FxxP_1	243	246	PF00568	0.476
DOC_PP4_FxxP_1	26	29	PF00568	0.662
DOC_USP7_MATH_1	179	183	PF00917	0.647
DOC_USP7_MATH_1	327	331	PF00917	0.525
DOC_USP7_MATH_1	68	72	PF00917	0.616
DOC_USP7_UBL2_3	30	34	PF12436	0.788
DOC_WW_Pin1_4	187	192	PF00397	0.569
DOC_WW_Pin1_4	25	30	PF00397	0.579
LIG_14-3-3_CanoR_1	156	160	PF00244	0.607
LIG_14-3-3_CanoR_1	186	193	PF00244	0.610
LIG_14-3-3_CanoR_1	23	29	PF00244	0.565
LIG_14-3-3_CanoR_1	317	322	PF00244	0.476
LIG_14-3-3_CanoR_1	371	377	PF00244	0.524
LIG_14-3-3_CanoR_1	7	13	PF00244	0.421
LIG_Actin_WH2_2	262	278	PF00022	0.525
LIG_Actin_WH2_2	336	353	PF00022	0.629
LIG_Actin_WH2_2	358	373	PF00022	0.589
LIG_BRCT_BRCA1_1	189	193	PF00533	0.555
LIG_BRCT_BRCA1_1	264	268	PF00533	0.501
LIG_BRCT_BRCA1_2	264	270	PF00533	0.501
LIG_Clathr_ClatBox_1	123	127	PF01394	0.598
LIG_DLG_GKlike_1	134	141	PF00625	0.625
LIG_EH1_1	381	389	PF00400	0.535
LIG_FHA_1	192	198	PF00498	0.484
LIG_FHA_1	216	222	PF00498	0.541
LIG_FHA_1	242	248	PF00498	0.470
LIG_FHA_1	3	9	PF00498	0.465
LIG_FHA_1	58	64	PF00498	0.568
LIG_FHA_2	225	231	PF00498	0.581
LIG_FHA_2	96	102	PF00498	0.600
LIG_LIR_Gen_1	265	275	PF02991	0.517
LIG_LIR_Nem_3	112	117	PF02991	0.559
LIG_LIR_Nem_3	235	240	PF02991	0.468
LIG_LIR_Nem_3	265	271	PF02991	0.476
LIG_NRBOX	5	11	PF00104	0.369
LIG_Pex14_2	110	114	PF04695	0.519
LIG_Pex14_2	79	83	PF04695	0.533
LIG_SH2_CRK	336	340	PF00017	0.497
LIG_SH2_STAP1	151	155	PF00017	0.617
LIG_SH2_STAP1	203	207	PF00017	0.505
LIG_SH2_STAT5	21	24	PF00017	0.543
LIG_SH2_STAT5	242	245	PF00017	0.476
LIG_SH2_STAT5	307	310	PF00017	0.471
LIG_SUMO_SIM_anti_2	120	125	PF11976	0.472
LIG_SUMO_SIM_par_1	115	120	PF11976	0.555
LIG_SUMO_SIM_par_1	122	127	PF11976	0.506
LIG_SUMO_SIM_par_1	8	13	PF11976	0.446
LIG_TRAF2_1	345	348	PF00917	0.578
LIG_TRAF2_1	99	102	PF00917	0.584
LIG_TYR_ITIM	240	245	PF00017	0.328
LIG_TYR_ITIM	334	339	PF00017	0.360
LIG_UBA3_1	304	312	PF00899	0.444
LIG_WRC_WIRS_1	202	207	PF05994	0.449
MOD_CDK_SPK_2	25	30	PF00069	0.473
MOD_CK1_1	173	179	PF00069	0.506
MOD_CK1_1	214	220	PF00069	0.397
MOD_CK2_1	95	101	PF00069	0.532
MOD_Cter_Amidation	43	46	PF01082	0.669
MOD_GlcNHglycan	213	216	PF01048	0.328
MOD_GlcNHglycan	330	333	PF01048	0.461
MOD_GSK3_1	146	153	PF00069	0.512
MOD_GSK3_1	170	177	PF00069	0.483
MOD_GSK3_1	185	192	PF00069	0.382
MOD_GSK3_1	201	208	PF00069	0.309
MOD_GSK3_1	210	217	PF00069	0.145
MOD_N-GLC_1	215	220	PF02516	0.328
MOD_N-GLC_2	62	64	PF02516	0.399
MOD_NEK2_1	110	115	PF00069	0.382
MOD_NEK2_1	155	160	PF00069	0.493
MOD_NEK2_1	205	210	PF00069	0.396
MOD_NEK2_1	211	216	PF00069	0.205
MOD_NEK2_1	8	13	PF00069	0.449
MOD_NEK2_2	151	156	PF00069	0.461
MOD_PKA_1	134	140	PF00069	0.514
MOD_PKA_2	134	140	PF00069	0.510
MOD_PKA_2	155	161	PF00069	0.503
MOD_PKA_2	185	191	PF00069	0.553
MOD_PKA_2	328	334	PF00069	0.463
MOD_Plk_1	180	186	PF00069	0.566
MOD_Plk_1	205	211	PF00069	0.432
MOD_Plk_1	68	74	PF00069	0.523
MOD_Plk_2-3	96	102	PF00069	0.534
MOD_Plk_4	310	316	PF00069	0.371
MOD_Plk_4	317	323	PF00069	0.308
MOD_ProDKin_1	187	193	PF00069	0.453
MOD_ProDKin_1	25	31	PF00069	0.480
TRG_DiLeu_BaEn_3	359	365	PF01217	0.473
TRG_DiLeu_BaEn_4	101	107	PF01217	0.421
TRG_DiLeu_BaLyEn_6	4	9	PF01217	0.526
TRG_ENDOCYTIC_2	242	245	PF00928	0.328
TRG_ENDOCYTIC_2	336	339	PF00928	0.355
TRG_ER_diArg_1	133	135	PF00400	0.515
TRG_ER_diArg_1	155	157	PF00400	0.542
TRG_ER_diArg_1	161	163	PF00400	0.553
TRG_ER_diArg_1	75	77	PF00400	0.407
TRG_NES_CRM1_1	355	369	PF08389	0.479
TRG_NLS_Bipartite_1	156	171	PF00514	0.507
TRG_NLS_Bipartite_1	23	38	PF00514	0.683
TRG_NLS_MonoExtC_3	33	38	PF00514	0.751

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4L5	Leptomonas seymouri	64%	97%
A0A0S4JHI6	Bodo saltans	49%	95%
A0A1X0P9M7	Trypanosomatidae	56%	95%
A0A422N7F4	Trypanosoma rangeli	53%	100%
A0A451EJL2	Leishmania donovani	94%	100%
A4H3H8	Leishmania braziliensis	84%	100%
D0A212	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	99%
E9AG25	Leishmania infantum	94%	100%
E9AJP1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
V5B8P6	Trypanosoma cruzi	51%	100%