Putative serine peptidase, Clan S-, family S54

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

serine peptidase, Clan S-, family S54, putative

Species:

Leishmania major

UniProt:

E9ACB8_LEIMA

TriTrypDb:

LmjF.02.0430 , LMJLV39_020009300 , LMJSD75_020009400

Length:

370

Annotations

LeishMANIAdb annotations

Despite the signature matches, it is both structurally and sequence-wise very dissimilar from rhomboids.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 5, no: 5
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	11
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

E9ACB8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ACB8

Function

Biological processes
Term	Name	Level	Count
GO:0005975	carbohydrate metabolic process	3	3
GO:0006066	alcohol metabolic process	3	3
GO:0006071	glycerol metabolic process	5	3
GO:0008152	metabolic process	1	3
GO:0009987	cellular process	1	3
GO:0019400	alditol metabolic process	4	3
GO:0019751	polyol metabolic process	4	3
GO:0044237	cellular metabolic process	2	3
GO:0044238	primary metabolic process	2	3
GO:0044262	obsolete cellular carbohydrate metabolic process	3	3
GO:0044281	small molecule metabolic process	2	3
GO:0071704	organic substance metabolic process	2	3
GO:1901615	organic hydroxy compound metabolic process	3	3

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	7
GO:0004371	glycerone kinase activity	5	3
GO:0016301	kinase activity	4	3
GO:0016740	transferase activity	2	3
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	3
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	3
GO:0004175	endopeptidase activity	4	4
GO:0004252	serine-type endopeptidase activity	5	4
GO:0008233	peptidase activity	3	4
GO:0008236	serine-type peptidase activity	4	4
GO:0016787	hydrolase activity	2	4
GO:0017171	serine hydrolase activity	3	4
GO:0140096	catalytic activity, acting on a protein	2	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	173	175	PF00675	0.365
CLV_NRD_NRD_1	298	300	PF00675	0.425
CLV_NRD_NRD_1	336	338	PF00675	0.615
CLV_NRD_NRD_1	340	342	PF00675	0.619
CLV_NRD_NRD_1	349	351	PF00675	0.601
CLV_NRD_NRD_1	98	100	PF00675	0.742
CLV_PCSK_KEX2_1	173	175	PF00082	0.311
CLV_PCSK_KEX2_1	188	190	PF00082	0.483
CLV_PCSK_KEX2_1	298	300	PF00082	0.425
CLV_PCSK_KEX2_1	340	342	PF00082	0.580
CLV_PCSK_KEX2_1	348	350	PF00082	0.595
CLV_PCSK_KEX2_1	97	99	PF00082	0.740
CLV_PCSK_PC1ET2_1	188	190	PF00082	0.352
CLV_PCSK_SKI1_1	128	132	PF00082	0.608
CLV_PCSK_SKI1_1	169	173	PF00082	0.282
CLV_PCSK_SKI1_1	256	260	PF00082	0.346
CLV_PCSK_SKI1_1	298	302	PF00082	0.427
CLV_PCSK_SKI1_1	55	59	PF00082	0.555
CLV_PCSK_SKI1_1	67	71	PF00082	0.566
CLV_PCSK_SKI1_1	98	102	PF00082	0.661
DOC_CYCLIN_RxL_1	337	347	PF00134	0.339
DOC_MAPK_gen_1	337	344	PF00069	0.334
DOC_MAPK_gen_1	38	48	PF00069	0.416
DOC_MAPK_gen_1	52	62	PF00069	0.270
DOC_MAPK_MEF2A_6	55	64	PF00069	0.296
DOC_MAPK_RevD_3	323	338	PF00069	0.282
DOC_PP2B_LxvP_1	263	266	PF13499	0.581
DOC_PP2B_LxvP_1	282	285	PF13499	0.613
DOC_USP7_MATH_1	108	112	PF00917	0.487
DOC_USP7_MATH_1	114	118	PF00917	0.456
DOC_USP7_MATH_1	217	221	PF00917	0.651
DOC_USP7_MATH_1	300	304	PF00917	0.618
DOC_WW_Pin1_4	11	16	PF00397	0.258
LIG_14-3-3_CanoR_1	189	194	PF00244	0.698
LIG_14-3-3_CanoR_1	256	265	PF00244	0.549
LIG_14-3-3_CanoR_1	299	309	PF00244	0.651
LIG_14-3-3_CanoR_1	41	47	PF00244	0.380
LIG_14-3-3_CanoR_1	55	64	PF00244	0.405
LIG_APCC_ABBA_1	46	51	PF00400	0.320
LIG_BRCT_BRCA1_1	65	69	PF00533	0.370
LIG_BRCT_BRCA1_1	70	74	PF00533	0.384
LIG_CSL_BTD_1	353	356	PF09270	0.345
LIG_FHA_1	154	160	PF00498	0.422
LIG_FHA_1	219	225	PF00498	0.654
LIG_FHA_1	270	276	PF00498	0.570
LIG_FHA_1	302	308	PF00498	0.656
LIG_FHA_2	29	35	PF00498	0.349
LIG_FHA_2	77	83	PF00498	0.281
LIG_FHA_2	87	93	PF00498	0.392
LIG_FHA_2	99	105	PF00498	0.461
LIG_LIR_Gen_1	259	269	PF02991	0.561
LIG_LIR_Gen_1	58	69	PF02991	0.363
LIG_LIR_Nem_3	259	264	PF02991	0.556
LIG_LIR_Nem_3	303	308	PF02991	0.655
LIG_LIR_Nem_3	58	64	PF02991	0.364
LIG_LIR_Nem_3	82	86	PF02991	0.444
LIG_PDZ_Class_1	365	370	PF00595	0.466
LIG_RPA_C_Fungi	169	181	PF08784	0.496
LIG_SH2_PTP2	279	282	PF00017	0.509
LIG_SH2_STAP1	292	296	PF00017	0.525
LIG_SH2_STAT5	279	282	PF00017	0.543
LIG_SH2_STAT5	331	334	PF00017	0.355
LIG_SH3_1	350	356	PF00018	0.408
LIG_SH3_3	350	356	PF00018	0.414
LIG_Sin3_3	261	268	PF02671	0.377
LIG_SUMO_SIM_anti_2	14	21	PF11976	0.271
LIG_SUMO_SIM_par_1	14	21	PF11976	0.363
LIG_SUMO_SIM_par_1	220	225	PF11976	0.393
LIG_TRAF2_1	225	228	PF00917	0.452
LIG_UBA3_1	60	67	PF00899	0.399
LIG_WRC_WIRS_1	165	170	PF05994	0.326
LIG_WRC_WIRS_1	73	78	PF05994	0.413
MOD_CK1_1	247	253	PF00069	0.426
MOD_CK2_1	222	228	PF00069	0.454
MOD_CK2_1	76	82	PF00069	0.328
MOD_CK2_1	86	92	PF00069	0.481
MOD_GlcNHglycan	105	111	PF01048	0.634
MOD_GlcNHglycan	117	120	PF01048	0.513
MOD_GlcNHglycan	142	145	PF01048	0.446
MOD_GlcNHglycan	224	227	PF01048	0.446
MOD_GlcNHglycan	239	242	PF01048	0.304
MOD_GlcNHglycan	246	249	PF01048	0.454
MOD_GlcNHglycan	25	28	PF01048	0.545
MOD_GSK3_1	18	25	PF00069	0.464
MOD_GSK3_1	218	225	PF00069	0.607
MOD_GSK3_1	68	75	PF00069	0.437
MOD_N-GLC_1	189	194	PF02516	0.436
MOD_N-GLC_1	218	223	PF02516	0.430
MOD_NEK2_1	164	169	PF00069	0.372
MOD_NEK2_1	222	227	PF00069	0.520
MOD_NEK2_1	301	306	PF00069	0.623
MOD_NEK2_1	344	349	PF00069	0.540
MOD_NEK2_1	76	81	PF00069	0.327
MOD_PKA_1	188	194	PF00069	0.438
MOD_PKA_1	98	104	PF00069	0.586
MOD_PKA_2	188	194	PF00069	0.369
MOD_PKA_2	98	104	PF00069	0.719
MOD_PKB_1	97	105	PF00069	0.564
MOD_Plk_1	189	195	PF00069	0.377
MOD_Plk_1	218	224	PF00069	0.424
MOD_ProDKin_1	11	17	PF00069	0.265
TRG_ENDOCYTIC_2	165	168	PF00928	0.493
TRG_ENDOCYTIC_2	279	282	PF00928	0.547
TRG_ENDOCYTIC_2	331	334	PF00928	0.475
TRG_ER_diArg_1	134	137	PF00400	0.507
TRG_ER_diArg_1	172	174	PF00400	0.439
TRG_ER_diArg_1	211	214	PF00400	0.446
TRG_ER_diArg_1	340	343	PF00400	0.472
TRG_ER_diArg_1	348	350	PF00400	0.518
TRG_ER_diArg_1	367	370	PF00400	0.517
TRG_ER_diArg_1	97	99	PF00400	0.586
TRG_NLS_MonoExtC_3	336	341	PF00514	0.521
TRG_PTS1	367	370	PF00515	0.521

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IG61	Leptomonas seymouri	54%	100%
A0A1X0NK02	Trypanosomatidae	32%	100%
A0A3R7KGS4	Trypanosoma rangeli	32%	100%
A0A3S5H501	Leishmania donovani	89%	100%
A4H3C4	Leishmania braziliensis	67%	99%
A4HRP0	Leishmania infantum	89%	100%
C9ZJ23	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	100%
E9AJK6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
V5BCY2	Trypanosoma cruzi	32%	71%