Putative ubiquitin-conjugating enzyme e2

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2, putative

Species:

Leishmania major

UniProt:

E9ACB4_LEIMA

TriTrypDb:

LmjF.02.0390 , LMJLV39_020008800 , LMJSD75_020008900

Length:

152

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9ACB4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ACB4

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0016925	protein sumoylation	7	2
GO:0018193	peptidyl-amino acid modification	5	2
GO:0018205	peptidyl-lysine modification	6	2
GO:0019538	protein metabolic process	3	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044238	primary metabolic process	2	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	10
GO:0003824	catalytic activity	1	10
GO:0005488	binding	1	10
GO:0005524	ATP binding	5	10
GO:0016740	transferase activity	2	10
GO:0017076	purine nucleotide binding	4	10
GO:0019787	ubiquitin-like protein transferase activity	3	10
GO:0019789	SUMO transferase activity	4	10
GO:0030554	adenyl nucleotide binding	5	10
GO:0032553	ribonucleotide binding	3	10
GO:0032555	purine ribonucleotide binding	4	10
GO:0032559	adenyl ribonucleotide binding	5	10
GO:0035639	purine ribonucleoside triphosphate binding	4	10
GO:0036094	small molecule binding	2	10
GO:0043167	ion binding	2	10
GO:0043168	anion binding	3	10
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	3
GO:0061656	SUMO conjugating enzyme activity	5	2
GO:0097159	organic cyclic compound binding	2	10
GO:0097367	carbohydrate derivative binding	2	10
GO:0140096	catalytic activity, acting on a protein	2	10
GO:1901265	nucleoside phosphate binding	3	10
GO:1901363	heterocyclic compound binding	2	10
GO:0004842	ubiquitin-protein transferase activity	4	1
GO:0016746	acyltransferase activity	3	1
GO:0016874	ligase activity	2	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_SKI1_1	103	107	PF00082	0.269
CLV_PCSK_SKI1_1	132	136	PF00082	0.395
CLV_PCSK_SKI1_1	67	71	PF00082	0.284
DOC_CKS1_1	42	47	PF01111	0.321
DOC_CKS1_1	69	74	PF01111	0.380
DOC_MAPK_gen_1	94	102	PF00069	0.260
DOC_MAPK_MEF2A_6	94	102	PF00069	0.287
DOC_PP1_RVXF_1	52	58	PF00149	0.376
DOC_PP2B_PxIxI_1	97	103	PF00149	0.260
DOC_USP7_MATH_1	17	21	PF00917	0.634
DOC_WW_Pin1_4	1	6	PF00397	0.711
DOC_WW_Pin1_4	41	46	PF00397	0.321
DOC_WW_Pin1_4	68	73	PF00397	0.388
LIG_14-3-3_CanoR_1	54	62	PF00244	0.401
LIG_FHA_1	69	75	PF00498	0.317
LIG_FHA_1	82	88	PF00498	0.199
LIG_FHA_2	42	48	PF00498	0.260
LIG_LIR_Nem_3	124	130	PF02991	0.260
LIG_RPA_C_Fungi	13	25	PF08784	0.512
LIG_SH2_STAP1	137	141	PF00017	0.260
LIG_SH3_2	62	67	PF14604	0.284
LIG_SH3_3	36	42	PF00018	0.284
LIG_SH3_3	59	65	PF00018	0.297
LIG_SH3_3	66	72	PF00018	0.300
LIG_SUMO_SIM_par_1	105	111	PF11976	0.260
LIG_TRAF2_1	108	111	PF00917	0.260
LIG_TRAF2_1	121	124	PF00917	0.260
MOD_CK1_1	4	10	PF00069	0.645
MOD_CK2_1	41	47	PF00069	0.260
MOD_GlcNHglycan	19	22	PF01048	0.508
MOD_GlcNHglycan	6	9	PF01048	0.648
MOD_GlcNHglycan	81	84	PF01048	0.283
MOD_GSK3_1	3	10	PF00069	0.563
MOD_GSK3_1	81	88	PF00069	0.280
MOD_N-GLC_1	55	60	PF02516	0.287
MOD_N-GLC_1	98	103	PF02516	0.376
MOD_NEK2_2	98	103	PF00069	0.260
MOD_PKA_2	17	23	PF00069	0.531
MOD_Plk_1	98	104	PF00069	0.376
MOD_Plk_4	85	91	PF00069	0.260
MOD_ProDKin_1	1	7	PF00069	0.709
MOD_ProDKin_1	41	47	PF00069	0.321
MOD_ProDKin_1	68	74	PF00069	0.388
TRG_ER_diArg_1	51	54	PF00400	0.406
TRG_Pf-PMV_PEXEL_1	132	136	PF00026	0.329

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P683	Leptomonas seymouri	39%	68%
A0A0N0P6R4	Leptomonas seymouri	37%	84%
A0A0N1I1I8	Leptomonas seymouri	35%	66%
A0A0N1I1Z1	Leptomonas seymouri	35%	98%
A0A0N1I707	Leptomonas seymouri	37%	100%
A0A0N1PAW7	Leptomonas seymouri	84%	72%
A0A0S4IJE0	Bodo saltans	42%	69%
A0A0S4J8W6	Bodo saltans	34%	100%
A0A0S4JGV6	Bodo saltans	33%	100%
A0A0S4JJB5	Bodo saltans	77%	86%
A0A0S4JQY0	Bodo saltans	33%	72%
A0A0S4JRZ8	Bodo saltans	36%	94%
A0A0S4JS17	Bodo saltans	36%	100%
A0A0S4KJ33	Bodo saltans	38%	100%
A0A1B0GUS4	Homo sapiens	30%	99%
A0A1X0NH68	Trypanosomatidae	37%	100%
A0A1X0NJQ5	Trypanosomatidae	30%	100%
A0A1X0NLS0	Trypanosomatidae	35%	100%
A0A1X0NPI5	Trypanosomatidae	39%	94%
A0A1X0NT81	Trypanosomatidae	40%	73%
A0A1X0NW44	Trypanosomatidae	33%	72%
A0A3Q8II46	Leishmania donovani	37%	100%
A0A3R7K708	Trypanosoma rangeli	36%	100%
A0A3R7L4E4	Trypanosoma rangeli	90%	67%
A0A3R7M279	Trypanosoma rangeli	28%	76%
A0A3S5H604	Leishmania donovani	36%	84%
A0A3S7WYK3	Leishmania donovani	32%	98%
A0A422MVJ2	Trypanosoma rangeli	36%	100%
A0A422MY24	Trypanosoma rangeli	38%	95%
A0A422NDI3	Trypanosoma rangeli	41%	72%
A0A422NJI9	Trypanosoma rangeli	33%	72%
A0A422P4A8	Trypanosoma rangeli	29%	100%
A0A451EJH5	Leishmania donovani	99%	100%
A1L3K1	Xenopus laevis	39%	68%
A3KN22	Bos taurus	29%	83%
A4H3D5	Leishmania braziliensis	94%	68%
A4H533	Leishmania braziliensis	37%	84%
A4HDS4	Leishmania braziliensis	35%	98%
A4HLY8	Leishmania braziliensis	33%	100%
A4HMF2	Leishmania braziliensis	37%	100%
A4HRN6	Leishmania infantum	99%	100%
A4HTB1	Leishmania infantum	36%	84%
A4I122	Leishmania infantum	32%	98%
A4IB24	Leishmania infantum	37%	100%
A5PJC4	Bos taurus	31%	99%
A5PKP9	Xenopus laevis	40%	100%
A7SE05	Nematostella vectensis	38%	75%
B3MQV3	Drosophila ananassae	39%	73%
B3NWW9	Drosophila erecta	39%	73%
B4H9W2	Drosophila persimilis	39%	73%
B4IF39	Drosophila sechellia	39%	73%
B4JKB7	Drosophila grimshawi	39%	73%
B4L7V4	Drosophila mojavensis	39%	74%
B4MA02	Drosophila virilis	39%	73%
B4N208	Drosophila willistoni	39%	73%
B4Q2J2	Drosophila yakuba	39%	73%
B4R6G1	Drosophila simulans	39%	73%
B5DFI8	Rattus norvegicus	39%	68%
B5DKM4	Drosophila pseudoobscura pseudoobscura	39%	73%
C1C3R6	Lithobates catesbeianus	36%	72%
C3Z724	Branchiostoma floridae	37%	71%
C9ZJ18	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	77%	77%
C9ZNM0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
C9ZRM5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	68%
C9ZY61	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
D0A9T7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	70%
D3ZDK2	Rattus norvegicus	40%	100%
E9AJK2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	99%
E9ALA2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	84%
E9AX59	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	98%
E9B609	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
O00102	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	36%	92%
O00103	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	36%	86%
O00762	Homo sapiens	38%	85%
O09181	Mesocricetus auratus	61%	96%
O13685	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	33%	100%
O42646	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	67%
O74196	Colletotrichum gloeosporioides	42%	100%
O74201	Candida albicans (strain SC5314 / ATCC MYA-2876)	43%	85%
O74549	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	33%	86%
O74810	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	70%
P06104	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	40%	88%
P0C8G3	African swine fever virus (isolate Pig/Kenya/KEN-50/1950)	33%	70%
P0C8G4	African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996)	35%	70%
P0C8G5	African swine fever virus (isolate Warthog/Namibia/Wart80/1980)	36%	69%
P0CS16	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	42%	90%
P0CS17	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	42%	90%
P15731	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	40%	100%
P15732	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	40%	100%
P16577	Triticum aestivum	36%	83%
P21734	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	36%	71%
P23566	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	41%	100%
P25153	Drosophila melanogaster	39%	100%
P25865	Arabidopsis thaliana	42%	100%
P25866	Triticum aestivum	41%	100%
P25867	Drosophila melanogaster	41%	100%
P25868	Triticum aestivum	34%	90%
P25869	African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983)	34%	71%
P27949	African swine fever virus (strain Badajoz 1971 Vero-adapted)	36%	71%
P28263	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	34%	70%
P29340	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	83%
P34477	Caenorhabditis elegans	34%	93%
P35128	Drosophila melanogaster	34%	100%
P35129	Caenorhabditis elegans	42%	100%
P35130	Medicago sativa	42%	100%
P35131	Arabidopsis thaliana	40%	100%
P35132	Arabidopsis thaliana	40%	100%
P35133	Arabidopsis thaliana	40%	100%
P35134	Arabidopsis thaliana	40%	100%
P35135	Solanum lycopersicum	40%	100%
P40984	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	65%	97%
P42745	Arabidopsis thaliana	42%	100%
P42746	Arabidopsis thaliana	40%	100%
P42747	Arabidopsis thaliana	35%	91%
P42748	Arabidopsis thaliana	33%	81%
P42749	Arabidopsis thaliana	33%	82%
P42750	Arabidopsis thaliana	31%	83%
P43102	Candida albicans	42%	100%
P46595	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	43%	100%
P49459	Homo sapiens	40%	100%
P50623	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	51%	97%
P51668	Homo sapiens	40%	100%
P51965	Homo sapiens	36%	79%
P52478	Caenorhabditis elegans	42%	79%
P52482	Mus musculus	36%	79%
P52483	Mus musculus	35%	73%
P52484	Caenorhabditis elegans	32%	71%
P52487	Drosophila melanogaster	30%	99%
P52490	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	33%	99%
P52492	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	37%	97%
P52493	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	41%	100%
P56616	Xenopus laevis	32%	85%
P60604	Homo sapiens	35%	92%
P60605	Mus musculus	35%	92%
P61077	Homo sapiens	41%	100%
P61078	Rattus norvegicus	41%	100%
P61079	Mus musculus	41%	100%
P61080	Mus musculus	40%	100%
P61081	Homo sapiens	29%	83%
P61082	Mus musculus	29%	83%
P62253	Homo sapiens	36%	89%
P62254	Mus musculus	36%	89%
P62255	Rattus norvegicus	36%	89%
P62256	Homo sapiens	33%	83%
P62257	Mus musculus	33%	83%
P62837	Homo sapiens	41%	100%
P62838	Mus musculus	41%	100%
P62839	Rattus norvegicus	41%	100%
P62840	Xenopus laevis	41%	100%
P63146	Homo sapiens	40%	100%
P63147	Mus musculus	40%	100%
P63148	Oryctolagus cuniculus	40%	100%
P63149	Rattus norvegicus	40%	100%
P63279	Homo sapiens	66%	96%
P63280	Mus musculus	66%	96%
P63281	Rattus norvegicus	66%	96%
P63282	Xenopus laevis	66%	96%
P63283	Gallus gallus	66%	96%
P68036	Homo sapiens	31%	99%
P68037	Mus musculus	31%	99%
P70711	Rattus norvegicus	40%	100%
P78717	Fusarium solani	41%	100%
Q02159	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	34%	92%
Q06AA9	Sus scrofa	41%	100%
Q16763	Homo sapiens	37%	68%
Q17QG5	Bos taurus	35%	92%
Q1RML1	Bos taurus	38%	68%
Q1RMW1	Bos taurus	28%	82%
Q1RMX2	Bos taurus	41%	100%
Q21633	Caenorhabditis elegans	32%	99%
Q28CQ4	Xenopus tropicalis	66%	96%
Q28F89	Xenopus tropicalis	36%	72%
Q2EF73	Ictidomys tridecemlineatus	66%	96%
Q2T9X7	Bos taurus	35%	73%
Q2TA10	Bos taurus	40%	100%
Q32LD2	Bos taurus	31%	78%
Q32LN1	Bos taurus	33%	83%
Q32P99	Bos taurus	40%	100%
Q32PA5	Bos taurus	37%	85%
Q3MHP1	Bos taurus	31%	99%
Q3UWQ3	Mus musculus	28%	82%
Q3ZCF7	Bos taurus	40%	100%
Q42540	Arabidopsis thaliana	34%	92%
Q42541	Arabidopsis thaliana	34%	92%
Q42551	Arabidopsis thaliana	57%	95%
Q4PFA5	Ustilago maydis (strain 521 / FGSC 9021)	43%	82%
Q4QAB7	Leishmania major	35%	98%
Q4QIK2	Leishmania major	36%	84%
Q4R5N4	Macaca fascicularis	41%	100%
Q4R5Y8	Macaca fascicularis	36%	89%
Q4R9D1	Macaca fascicularis	37%	85%
Q4V908	Danio rerio	36%	69%
Q4WLA7	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	42%	100%
Q54I43	Dictyostelium discoideum	38%	71%
Q54TI6	Dictyostelium discoideum	28%	66%
Q553F3	Dictyostelium discoideum	39%	100%
Q58FS2	Trichoderma harzianum	41%	100%
Q5M8Y2	Xenopus tropicalis	30%	82%
Q5R4V7	Pongo abelii	41%	100%
Q5R5I4	Pongo abelii	31%	99%
Q5RF84	Pongo abelii	35%	92%
Q5U203	Rattus norvegicus	29%	82%
Q5UQC9	Acanthamoeba polyphaga mimivirus	31%	96%
Q5ZKX6	Gallus gallus	28%	82%
Q6BU36	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	44%	90%
Q6C093	Yarrowia lipolytica (strain CLIB 122 / E 150)	42%	100%
Q6C9W0	Yarrowia lipolytica (strain CLIB 122 / E 150)	28%	85%
Q6CSW8	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	29%	83%
Q6CUD9	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	39%	93%
Q6DCZ9	Xenopus laevis	30%	83%
Q6FR76	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	40%	91%
Q6IRC7	Xenopus laevis	30%	82%
Q6NY82	Danio rerio	25%	82%
Q6P8D9	Xenopus tropicalis	30%	83%
Q6Y1Z4	Pagrus major	66%	96%
Q6ZWY6	Mus musculus	40%	100%
Q75AF2	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	29%	83%
Q75EB8	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	39%	89%
Q76EZ2	Xenopus laevis	35%	72%
Q7K738	Drosophila melanogaster	31%	99%
Q86K32	Dictyostelium discoideum	38%	99%
Q8AVU2	Xenopus laevis	37%	72%
Q8L7T3	Arabidopsis thaliana	38%	84%
Q8S919	Oryza sativa subsp. japonica	38%	100%
Q8S920	Oryza sativa subsp. japonica	40%	100%
Q8SSK3	Encephalitozoon cuniculi (strain GB-M1)	32%	70%
Q91W82	Mus musculus	36%	76%
Q921J4	Mus musculus	39%	68%
Q94490	Dictyostelium discoideum	29%	100%
Q94A97	Arabidopsis thaliana	38%	99%
Q94F47	Arabidopsis thaliana	40%	100%
Q95017	Caenorhabditis elegans	58%	92%
Q95044	Spisula solidissima	41%	86%
Q969M7	Homo sapiens	28%	82%
Q969T4	Homo sapiens	35%	73%
Q96LR5	Homo sapiens	36%	76%
Q96UP5	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	42%	100%
Q9C8X7	Arabidopsis thaliana	35%	99%
Q9C9Y7	Arabidopsis thaliana	39%	100%
Q9CY34	Mus musculus	29%	82%
Q9D1C1	Mus musculus	37%	85%
Q9DDJ0	Danio rerio	66%	97%
Q9FI61	Arabidopsis thaliana	35%	79%
Q9FKT3	Arabidopsis thaliana	38%	100%
Q9FZ48	Arabidopsis thaliana	38%	99%
Q9GNC1	Leishmania major	37%	100%
Q9LJD7	Arabidopsis thaliana	37%	84%
Q9LJZ5	Arabidopsis thaliana	36%	84%
Q9NGP4	Dictyostelium discoideum	56%	96%
Q9NPD8	Homo sapiens	31%	77%
Q9P6I1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	34%	95%
Q9P7R4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	83%
Q9SDY5	Arabidopsis thaliana	31%	83%
Q9SLE4	Arabidopsis thaliana	41%	100%
Q9UTN8	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	33%	98%
Q9UVR2	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	41%	100%
Q9VSF3	Drosophila melanogaster	30%	84%
Q9VTY6	Drosophila melanogaster	33%	85%
Q9VX25	Drosophila melanogaster	39%	73%
Q9W6H5	Danio rerio	66%	96%
Q9XVK5	Caenorhabditis elegans	24%	84%
Q9Y2X8	Homo sapiens	40%	100%
Q9Y818	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	36%	91%
Q9Z255	Mus musculus	40%	100%
Q9ZU75	Arabidopsis thaliana	31%	82%
V5BDP3	Trypanosoma cruzi	35%	100%
V5BRP1	Trypanosoma cruzi	29%	100%
V5BVS1	Trypanosoma cruzi	42%	68%
V5BYE2	Trypanosoma cruzi	39%	95%