Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

FtsJ-like methyltransferase, putative

Species:

Leishmania major

UniProt:

E9ACB3_LEIMA

TriTrypDb:

LmjF.02.0380 , LMJLV39_020008700 , LMJSD75_020008800

Length:

459

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 14
NetGPI	no	yes: 0, no: 14

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	10
GO:0110165	cellular anatomical entity	1	11
GO:0005730	nucleolus	5	1
GO:0030684	preribosome	3	1
GO:0030687	preribosome, large subunit precursor	4	1
GO:0032991	protein-containing complex	1	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:1990904	ribonucleoprotein complex	2	1

Expansion

Sequence features

E9ACB3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ACB3

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	11
GO:0002128	tRNA nucleoside ribose methylation	6	10
GO:0002181	cytoplasmic translation	5	10
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006396	RNA processing	6	11
GO:0006399	tRNA metabolic process	7	10
GO:0006400	tRNA modification	6	10
GO:0006412	translation	4	10
GO:0006518	peptide metabolic process	4	10
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008033	tRNA processing	8	10
GO:0008152	metabolic process	1	15
GO:0009058	biosynthetic process	2	10
GO:0009059	macromolecule biosynthetic process	4	10
GO:0009451	RNA modification	5	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0019538	protein metabolic process	3	10
GO:0030488	tRNA methylation	5	10
GO:0032259	methylation	2	15
GO:0034470	ncRNA processing	7	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034645	obsolete cellular macromolecule biosynthetic process	4	10
GO:0034660	ncRNA metabolic process	6	11
GO:0043043	peptide biosynthetic process	5	10
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0043414	macromolecule methylation	3	11
GO:0043603	amide metabolic process	3	10
GO:0043604	amide biosynthetic process	4	10
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	10
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	10
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901564	organonitrogen compound metabolic process	3	10
GO:1901566	organonitrogen compound biosynthetic process	4	10
GO:1901576	organic substance biosynthetic process	3	10
GO:0000154	rRNA modification	6	1
GO:0000460	maturation of 5.8S rRNA	9	1
GO:0000463	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	10	1
GO:0000466	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	10	1
GO:0000470	maturation of LSU-rRNA	9	1
GO:0006364	rRNA processing	8	1
GO:0016072	rRNA metabolic process	7	1
GO:0031167	rRNA methylation	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	15
GO:0008168	methyltransferase activity	4	15
GO:0008173	RNA methyltransferase activity	4	11
GO:0008175	tRNA methyltransferase activity	5	10
GO:0016740	transferase activity	2	15
GO:0016741	transferase activity, transferring one-carbon groups	3	15
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140101	catalytic activity, acting on a tRNA	4	10
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:0008171	O-methyltransferase activity	5	1
GO:0008649	rRNA methyltransferase activity	5	1
GO:0008650	rRNA (uridine-2'-O-)-methyltransferase activity	6	1
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	1
GO:0016435	rRNA (guanine) methyltransferase activity	6	1
GO:0016436	rRNA (uridine) methyltransferase activity	6	1
GO:0062105	RNA 2'-O-methyltransferase activity	5	1
GO:0140102	catalytic activity, acting on a rRNA	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	364	368	PF00656	0.527
CLV_C14_Caspase3-7	428	432	PF00656	0.681
CLV_C14_Caspase3-7	77	81	PF00656	0.406
CLV_NRD_NRD_1	13	15	PF00675	0.406
CLV_NRD_NRD_1	161	163	PF00675	0.308
CLV_PCSK_KEX2_1	8	10	PF00082	0.458
CLV_PCSK_PC1ET2_1	8	10	PF00082	0.458
CLV_PCSK_SKI1_1	14	18	PF00082	0.388
CLV_PCSK_SKI1_1	232	236	PF00082	0.329
DEG_APCC_DBOX_1	253	261	PF00400	0.382
DEG_SPOP_SBC_1	125	129	PF00917	0.429
DEG_SPOP_SBC_1	143	147	PF00917	0.545
DOC_CYCLIN_yCln2_LP_2	404	410	PF00134	0.620
DOC_MAPK_gen_1	162	168	PF00069	0.483
DOC_PP2B_LxvP_1	123	126	PF13499	0.548
DOC_PP2B_LxvP_1	404	407	PF13499	0.577
DOC_PP4_FxxP_1	212	215	PF00568	0.465
DOC_PP4_FxxP_1	253	256	PF00568	0.383
DOC_USP7_MATH_1	143	147	PF00917	0.581
DOC_USP7_MATH_1	236	240	PF00917	0.505
DOC_USP7_MATH_1	270	274	PF00917	0.642
DOC_USP7_MATH_1	284	288	PF00917	0.783
DOC_USP7_MATH_1	310	314	PF00917	0.644
DOC_USP7_MATH_1	414	418	PF00917	0.657
DOC_USP7_MATH_1	430	434	PF00917	0.608
DOC_WW_Pin1_4	151	156	PF00397	0.501
DOC_WW_Pin1_4	157	162	PF00397	0.465
DOC_WW_Pin1_4	320	325	PF00397	0.684
DOC_WW_Pin1_4	412	417	PF00397	0.505
DOC_WW_Pin1_4	439	444	PF00397	0.601
DOC_WW_Pin1_4	83	88	PF00397	0.567
LIG_14-3-3_CanoR_1	213	221	PF00244	0.518
LIG_14-3-3_CanoR_1	22	30	PF00244	0.502
LIG_14-3-3_CanoR_1	240	245	PF00244	0.471
LIG_14-3-3_CanoR_1	360	366	PF00244	0.634
LIG_Actin_WH2_2	187	204	PF00022	0.475
LIG_APCC_ABBA_1	30	35	PF00400	0.457
LIG_BRCT_BRCA1_1	144	148	PF00533	0.421
LIG_BRCT_BRCA1_1	223	227	PF00533	0.486
LIG_eIF4E_1	184	190	PF01652	0.465
LIG_FHA_1	109	115	PF00498	0.553
LIG_FHA_1	127	133	PF00498	0.410
LIG_FHA_1	195	201	PF00498	0.487
LIG_FHA_1	215	221	PF00498	0.481
LIG_FHA_1	44	50	PF00498	0.467
LIG_FHA_1	84	90	PF00498	0.492
LIG_FHA_2	113	119	PF00498	0.465
LIG_FHA_2	132	138	PF00498	0.465
LIG_FHA_2	348	354	PF00498	0.608
LIG_FHA_2	362	368	PF00498	0.674
LIG_FHA_2	426	432	PF00498	0.608
LIG_FHA_2	451	457	PF00498	0.627
LIG_LIR_Apic_2	209	215	PF02991	0.465
LIG_LIR_Gen_1	34	44	PF02991	0.483
LIG_LIR_Gen_1	57	66	PF02991	0.465
LIG_LIR_Nem_3	25	30	PF02991	0.489
LIG_LIR_Nem_3	34	39	PF02991	0.537
LIG_LIR_Nem_3	57	61	PF02991	0.465
LIG_PCNA_PIPBox_1	178	187	PF02747	0.483
LIG_PCNA_yPIPBox_3	235	248	PF02747	0.465
LIG_SH2_CRK	27	31	PF00017	0.465
LIG_SH2_STAP1	331	335	PF00017	0.648
LIG_SH2_STAT5	184	187	PF00017	0.465
LIG_SH2_STAT5	259	262	PF00017	0.532
LIG_SH2_STAT5	329	332	PF00017	0.689
LIG_SH2_STAT5	408	411	PF00017	0.547
LIG_SH3_3	230	236	PF00018	0.548
LIG_SH3_3	321	327	PF00018	0.570
LIG_SH3_3	366	372	PF00018	0.505
LIG_SH3_3	393	399	PF00018	0.554
LIG_SH3_3	437	443	PF00018	0.703
LIG_SUMO_SIM_anti_2	101	107	PF11976	0.492
LIG_SUMO_SIM_par_1	88	93	PF11976	0.506
LIG_TRAF2_1	16	19	PF00917	0.387
LIG_WRC_WIRS_1	196	201	PF05994	0.465
LIG_WRC_WIRS_1	244	249	PF05994	0.548
MOD_CDC14_SPxK_1	160	163	PF00782	0.524
MOD_CDK_SPK_2	157	162	PF00069	0.524
MOD_CDK_SPK_2	83	88	PF00069	0.492
MOD_CDK_SPxK_1	157	163	PF00069	0.524
MOD_CK1_1	112	118	PF00069	0.485
MOD_CK1_1	146	152	PF00069	0.539
MOD_CK1_1	243	249	PF00069	0.545
MOD_CK1_1	273	279	PF00069	0.696
MOD_CK1_1	320	326	PF00069	0.600
MOD_CK1_1	338	344	PF00069	0.684
MOD_CK2_1	112	118	PF00069	0.465
MOD_CK2_1	131	137	PF00069	0.465
MOD_CK2_1	289	295	PF00069	0.633
MOD_CK2_1	450	456	PF00069	0.629
MOD_DYRK1A_RPxSP_1	439	443	PF00069	0.600
MOD_GlcNHglycan	150	153	PF01048	0.346
MOD_GlcNHglycan	24	27	PF01048	0.298
MOD_GlcNHglycan	273	276	PF01048	0.668
MOD_GlcNHglycan	284	287	PF01048	0.659
MOD_GlcNHglycan	312	315	PF01048	0.782
MOD_GlcNHglycan	319	322	PF01048	0.703
MOD_GlcNHglycan	378	382	PF01048	0.499
MOD_GlcNHglycan	69	72	PF01048	0.293
MOD_GSK3_1	108	115	PF00069	0.466
MOD_GSK3_1	142	149	PF00069	0.515
MOD_GSK3_1	236	243	PF00069	0.506
MOD_GSK3_1	270	277	PF00069	0.622
MOD_GSK3_1	306	313	PF00069	0.690
MOD_GSK3_1	331	338	PF00069	0.665
MOD_GSK3_1	67	74	PF00069	0.469
MOD_LATS_1	65	71	PF00433	0.406
MOD_N-GLC_1	240	245	PF02516	0.265
MOD_N-GLC_1	71	76	PF02516	0.278
MOD_NEK2_1	148	153	PF00069	0.461
MOD_NEK2_1	194	199	PF00069	0.480
MOD_NEK2_1	317	322	PF00069	0.619
MOD_NEK2_2	347	352	PF00069	0.580
MOD_PIKK_1	221	227	PF00454	0.492
MOD_PIKK_1	414	420	PF00454	0.523
MOD_PIKK_1	430	436	PF00454	0.632
MOD_PIKK_1	56	62	PF00454	0.465
MOD_PKA_2	2	8	PF00069	0.522
MOD_PKA_2	317	323	PF00069	0.599
MOD_PKA_2	359	365	PF00069	0.683
MOD_PKA_2	425	431	PF00069	0.612
MOD_PKA_2	448	454	PF00069	0.626
MOD_Plk_1	240	246	PF00069	0.465
MOD_Plk_4	101	107	PF00069	0.591
MOD_Plk_4	195	201	PF00069	0.489
MOD_Plk_4	243	249	PF00069	0.538
MOD_ProDKin_1	151	157	PF00069	0.501
MOD_ProDKin_1	320	326	PF00069	0.678
MOD_ProDKin_1	412	418	PF00069	0.510
MOD_ProDKin_1	439	445	PF00069	0.601
MOD_ProDKin_1	83	89	PF00069	0.567
MOD_SUMO_for_1	16	19	PF00179	0.387
MOD_SUMO_for_1	302	305	PF00179	0.607
TRG_DiLeu_BaEn_1	118	123	PF01217	0.503
TRG_DiLeu_BaEn_4	34	40	PF01217	0.483
TRG_DiLeu_LyEn_5	118	123	PF01217	0.483
TRG_ENDOCYTIC_2	27	30	PF00928	0.482
TRG_ER_diArg_1	21	24	PF00400	0.459
TRG_NES_CRM1_1	95	109	PF08389	0.465
TRG_NLS_MonoExtC_3	434	439	PF00514	0.677
TRG_NLS_MonoExtN_4	435	440	PF00514	0.722

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HTF7	Leptomonas seymouri	61%	100%
A0A0S4JD71	Bodo saltans	44%	100%
A0A3S7X469	Leishmania donovani	25%	100%
A0A451EJH4	Leishmania donovani	95%	100%
A4H3D4	Leishmania braziliensis	76%	100%
A4HRN5	Leishmania infantum	95%	100%
A4I6F9	Leishmania infantum	25%	100%
C9ZJ17	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	100%
E9AIU3	Leishmania braziliensis	23%	100%
E9AJK1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
E9B1L6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	100%
Q9BHC8	Leishmania major	24%	100%
V5BCX7	Trypanosoma cruzi	50%	100%