Protein kinase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Serine/threonine-protein kinase NEK22, putative

Species:

Leishmania major

UniProt:

E9ACA4_LEIMA

TriTrypDb:

LmjF.02.0290 , LMJLV39_020007800 , LMJSD75_020007800

Length:

530

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 23
NetGPI	no	yes: 0, no: 23

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9ACA4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ACA4

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	24
GO:0006793	phosphorus metabolic process	3	24
GO:0006796	phosphate-containing compound metabolic process	4	24
GO:0006807	nitrogen compound metabolic process	2	24
GO:0008152	metabolic process	1	24
GO:0009987	cellular process	1	24
GO:0016310	phosphorylation	5	24
GO:0019538	protein metabolic process	3	24
GO:0036211	protein modification process	4	24
GO:0043170	macromolecule metabolic process	3	24
GO:0043412	macromolecule modification	4	24
GO:0044237	cellular metabolic process	2	24
GO:0044238	primary metabolic process	2	24
GO:0071704	organic substance metabolic process	2	24
GO:1901564	organonitrogen compound metabolic process	3	24

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	24
GO:0003824	catalytic activity	1	24
GO:0004672	protein kinase activity	3	24
GO:0005488	binding	1	24
GO:0005524	ATP binding	5	24
GO:0016301	kinase activity	4	24
GO:0016740	transferase activity	2	24
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	24
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	24
GO:0017076	purine nucleotide binding	4	24
GO:0030554	adenyl nucleotide binding	5	24
GO:0032553	ribonucleotide binding	3	24
GO:0032555	purine ribonucleotide binding	4	24
GO:0032559	adenyl ribonucleotide binding	5	24
GO:0035639	purine ribonucleoside triphosphate binding	4	24
GO:0036094	small molecule binding	2	24
GO:0043167	ion binding	2	24
GO:0043168	anion binding	3	24
GO:0097159	organic cyclic compound binding	2	24
GO:0097367	carbohydrate derivative binding	2	24
GO:0140096	catalytic activity, acting on a protein	2	24
GO:1901265	nucleoside phosphate binding	3	24
GO:1901363	heterocyclic compound binding	2	24
GO:0004674	protein serine/threonine kinase activity	4	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	168	172	PF00656	0.211
CLV_NRD_NRD_1	256	258	PF00675	0.242
CLV_NRD_NRD_1	448	450	PF00675	0.266
CLV_PCSK_KEX2_1	256	258	PF00082	0.293
CLV_PCSK_KEX2_1	448	450	PF00082	0.264
CLV_PCSK_PC7_1	444	450	PF00082	0.260
CLV_PCSK_SKI1_1	211	215	PF00082	0.227
CLV_PCSK_SKI1_1	317	321	PF00082	0.337
CLV_PCSK_SKI1_1	427	431	PF00082	0.377
CLV_PCSK_SKI1_1	448	452	PF00082	0.267
DEG_APCC_DBOX_1	443	451	PF00400	0.305
DEG_APCC_DBOX_1	456	464	PF00400	0.338
DEG_Nend_UBRbox_2	1	3	PF02207	0.498
DEG_SIAH_1	73	81	PF03145	0.476
DEG_SPOP_SBC_1	258	262	PF00917	0.247
DEG_SPOP_SBC_1	486	490	PF00917	0.467
DOC_ANK_TNKS_1	491	498	PF00023	0.394
DOC_CDC14_PxL_1	359	367	PF14671	0.307
DOC_CYCLIN_RxL_1	387	398	PF00134	0.230
DOC_MAPK_gen_1	317	326	PF00069	0.328
DOC_USP7_MATH_1	121	125	PF00917	0.589
DOC_USP7_MATH_1	304	308	PF00917	0.311
DOC_USP7_MATH_1	46	50	PF00917	0.615
DOC_USP7_MATH_1	484	488	PF00917	0.663
DOC_USP7_MATH_1	522	526	PF00917	0.470
DOC_USP7_MATH_1	54	58	PF00917	0.640
DOC_WW_Pin1_4	266	271	PF00397	0.288
DOC_WW_Pin1_4	354	359	PF00397	0.321
DOC_WW_Pin1_4	409	414	PF00397	0.317
DOC_WW_Pin1_4	482	487	PF00397	0.617
DOC_WW_Pin1_4	63	68	PF00397	0.552
DOC_WW_Pin1_4	88	93	PF00397	0.650
LIG_14-3-3_CanoR_1	492	499	PF00244	0.596
LIG_14-3-3_CterR_2	526	530	PF00244	0.514
LIG_BRCT_BRCA1_1	425	429	PF00533	0.286
LIG_BRCT_BRCA1_1	489	493	PF00533	0.648
LIG_deltaCOP1_diTrp_1	376	384	PF00928	0.311
LIG_deltaCOP1_diTrp_1	6	10	PF00928	0.599
LIG_eIF4E_1	360	366	PF01652	0.243
LIG_FHA_1	105	111	PF00498	0.535
LIG_FHA_1	121	127	PF00498	0.433
LIG_FHA_1	178	184	PF00498	0.304
LIG_FHA_1	330	336	PF00498	0.298
LIG_FHA_2	25	31	PF00498	0.733
LIG_FHA_2	340	346	PF00498	0.311
LIG_FHA_2	348	354	PF00498	0.311
LIG_FHA_2	7	13	PF00498	0.656
LIG_HOMEOBOX	5	8	PF00046	0.516
LIG_LIR_Apic_2	357	363	PF02991	0.321
LIG_LIR_Gen_1	2	10	PF02991	0.619
LIG_LIR_Gen_1	237	245	PF02991	0.316
LIG_LIR_Gen_1	296	305	PF02991	0.336
LIG_LIR_Gen_1	496	507	PF02991	0.332
LIG_LIR_Gen_1	66	76	PF02991	0.459
LIG_LIR_Nem_3	146	150	PF02991	0.402
LIG_LIR_Nem_3	296	301	PF02991	0.478
LIG_LIR_Nem_3	310	315	PF02991	0.220
LIG_LIR_Nem_3	426	432	PF02991	0.286
LIG_LIR_Nem_3	459	465	PF02991	0.332
LIG_LIR_Nem_3	496	502	PF02991	0.444
LIG_LIR_Nem_3	6	10	PF02991	0.599
LIG_LIR_Nem_3	66	72	PF02991	0.463
LIG_Rb_pABgroove_1	221	229	PF01858	0.311
LIG_SH2_CRK	312	316	PF00017	0.311
LIG_SH2_CRK	499	503	PF00017	0.294
LIG_SH2_PTP2	462	465	PF00017	0.243
LIG_SH2_SRC	222	225	PF00017	0.256
LIG_SH2_SRC	462	465	PF00017	0.275
LIG_SH2_STAP1	425	429	PF00017	0.379
LIG_SH2_STAP1	499	503	PF00017	0.260
LIG_SH2_STAT3	128	131	PF00017	0.497
LIG_SH2_STAT3	425	428	PF00017	0.311
LIG_SH2_STAT5	222	225	PF00017	0.297
LIG_SH2_STAT5	243	246	PF00017	0.298
LIG_SH2_STAT5	455	458	PF00017	0.323
LIG_SH2_STAT5	462	465	PF00017	0.328
LIG_SH3_3	229	235	PF00018	0.471
LIG_SH3_3	264	270	PF00018	0.248
LIG_SUMO_SIM_par_1	463	469	PF11976	0.360
LIG_TRAF2_1	196	199	PF00917	0.227
LIG_TYR_ITIM	460	465	PF00017	0.242
LIG_WW_1	231	234	PF00397	0.211
MOD_CK1_1	143	149	PF00069	0.463
MOD_CK1_1	260	266	PF00069	0.333
MOD_CK1_1	269	275	PF00069	0.347
MOD_CK1_1	307	313	PF00069	0.311
MOD_CK1_1	423	429	PF00069	0.432
MOD_CK1_1	435	441	PF00069	0.237
MOD_CK1_1	466	472	PF00069	0.374
MOD_CK1_1	487	493	PF00069	0.697
MOD_CK1_1	49	55	PF00069	0.640
MOD_CK1_1	88	94	PF00069	0.541
MOD_CK2_1	347	353	PF00069	0.367
MOD_CK2_1	395	401	PF00069	0.387
MOD_Cter_Amidation	254	257	PF01082	0.211
MOD_GlcNHglycan	12	17	PF01048	0.747
MOD_GlcNHglycan	123	126	PF01048	0.671
MOD_GlcNHglycan	132	135	PF01048	0.454
MOD_GlcNHglycan	142	145	PF01048	0.400
MOD_GlcNHglycan	173	176	PF01048	0.403
MOD_GlcNHglycan	20	23	PF01048	0.645
MOD_GlcNHglycan	397	400	PF01048	0.346
MOD_GlcNHglycan	493	496	PF01048	0.454
MOD_GlcNHglycan	54	57	PF01048	0.710
MOD_GSK3_1	226	233	PF00069	0.243
MOD_GSK3_1	265	272	PF00069	0.372
MOD_GSK3_1	274	281	PF00069	0.487
MOD_GSK3_1	419	426	PF00069	0.252
MOD_GSK3_1	431	438	PF00069	0.382
MOD_GSK3_1	478	485	PF00069	0.757
MOD_GSK3_1	487	494	PF00069	0.645
MOD_GSK3_1	498	505	PF00069	0.261
MOD_GSK3_1	522	529	PF00069	0.545
MOD_GSK3_1	84	91	PF00069	0.613
MOD_N-GLC_1	418	423	PF02516	0.297
MOD_N-GLC_1	63	68	PF02516	0.455
MOD_NEK2_1	226	231	PF00069	0.425
MOD_NEK2_1	275	280	PF00069	0.372
MOD_NEK2_1	338	343	PF00069	0.312
MOD_NEK2_1	432	437	PF00069	0.250
MOD_NEK2_1	498	503	PF00069	0.361
MOD_NEK2_2	162	167	PF00069	0.257
MOD_NEK2_2	293	298	PF00069	0.387
MOD_NEK2_2	304	309	PF00069	0.257
MOD_NEK2_2	94	99	PF00069	0.525
MOD_PIKK_1	339	345	PF00454	0.227
MOD_PIKK_1	487	493	PF00454	0.674
MOD_PIKK_1	510	516	PF00454	0.310
MOD_PKA_2	423	429	PF00069	0.450
MOD_PKA_2	443	449	PF00069	0.324
MOD_PKA_2	456	462	PF00069	0.328
MOD_PKA_2	491	497	PF00069	0.695
MOD_Plk_2-3	6	12	PF00069	0.492
MOD_Plk_4	162	168	PF00069	0.323
MOD_Plk_4	189	195	PF00069	0.319
MOD_Plk_4	234	240	PF00069	0.389
MOD_Plk_4	293	299	PF00069	0.326
MOD_Plk_4	432	438	PF00069	0.231
MOD_Plk_4	463	469	PF00069	0.364
MOD_Plk_4	498	504	PF00069	0.314
MOD_ProDKin_1	266	272	PF00069	0.288
MOD_ProDKin_1	354	360	PF00069	0.321
MOD_ProDKin_1	409	415	PF00069	0.317
MOD_ProDKin_1	482	488	PF00069	0.602
MOD_ProDKin_1	63	69	PF00069	0.553
MOD_ProDKin_1	88	94	PF00069	0.654
TRG_DiLeu_BaEn_1	401	406	PF01217	0.227
TRG_ENDOCYTIC_2	312	315	PF00928	0.311
TRG_ENDOCYTIC_2	385	388	PF00928	0.346
TRG_ENDOCYTIC_2	462	465	PF00928	0.243
TRG_ENDOCYTIC_2	499	502	PF00928	0.313
TRG_ER_diArg_1	152	155	PF00400	0.277
TRG_ER_diArg_1	256	258	PF00400	0.254
TRG_ER_diArg_1	447	449	PF00400	0.262
TRG_NES_CRM1_1	329	343	PF08389	0.227

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2D3	Leptomonas seymouri	52%	100%
A0A0N1I9A0	Leptomonas seymouri	27%	82%
A0A0S4IX86	Bodo saltans	29%	100%
A0A0S4J757	Bodo saltans	26%	98%
A0A0S4JCI5	Bodo saltans	27%	83%
A0A0S4JIJ6	Bodo saltans	26%	94%
A0A0S4JMY6	Bodo saltans	24%	69%
A0A1X0NFW4	Trypanosomatidae	28%	100%
A0A1X0NKA1	Trypanosomatidae	38%	100%
A0A3Q8IAQ1	Leishmania donovani	33%	100%
A0A3Q8IDY1	Leishmania donovani	28%	100%
A0A3Q8IFK8	Leishmania donovani	29%	100%
A0A3Q8IFW0	Leishmania donovani	32%	100%
A0A3Q8IIG1	Leishmania donovani	29%	100%
A0A3Q8IPJ5	Leishmania donovani	26%	100%
A0A3S5H4Z4	Leishmania donovani	94%	100%
A0A3S5H789	Leishmania donovani	27%	100%
A0A3S7WWE7	Leishmania donovani	28%	100%
A0A3S7X6T8	Leishmania donovani	28%	100%
A0A422N8E7	Trypanosoma rangeli	27%	68%
A0A422NT89	Trypanosoma rangeli	23%	81%
A4H3C7	Leishmania braziliensis	76%	100%
A4HBL4	Leishmania braziliensis	27%	100%
A4HCE6	Leishmania braziliensis	32%	100%
A4HFA1	Leishmania braziliensis	26%	100%
A4HHP8	Leishmania braziliensis	28%	100%
A4HJT5	Leishmania braziliensis	28%	100%
A4HJW2	Leishmania braziliensis	32%	100%
A4HLR0	Leishmania braziliensis	28%	100%
A4HRM6	Leishmania infantum	94%	100%
A4HW76	Leishmania infantum	25%	66%
A4HYX6	Leishmania infantum	27%	100%
A4HZA2	Leishmania infantum	28%	100%
A4HZW8	Leishmania infantum	33%	100%
A4I2H8	Leishmania infantum	26%	100%
A4I435	Leishmania infantum	29%	100%
A4I4W3	Leishmania infantum	28%	100%
A4I7A1	Leishmania infantum	29%	100%
A4I7C4	Leishmania infantum	32%	100%
A4I960	Leishmania infantum	28%	100%
C9ZIZ0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
C9ZQL9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	83%
E9AJJ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	98%
E9AUY2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9AVS7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E9AYN8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9B0C2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9B296	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
E9B2B7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9B436	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
F4JBP3	Arabidopsis thaliana	28%	75%
P0C8M8	Zea mays	25%	85%
P22209	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	100%
P31748	AKT8 murine leukemia virus	24%	100%
P31750	Mus musculus	24%	100%
P34101	Dictyostelium discoideum	23%	89%
P38147	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	100%
P47196	Rattus norvegicus	24%	100%
Q01314	Bos taurus	24%	100%
Q06850	Arabidopsis thaliana	27%	87%
Q07832	Mus musculus	27%	88%
Q38870	Arabidopsis thaliana	25%	82%
Q4Q3Y9	Leishmania major	28%	100%
Q4Q5T9	Leishmania major	32%	100%
Q4Q5W2	Leishmania major	29%	100%
Q4Q7W2	Leishmania major	29%	100%
Q4Q8T4	Leishmania major	26%	100%
Q4QBQ2	Leishmania major	34%	100%
Q4QCK0	Leishmania major	27%	100%
Q5KQF5	Oryza sativa subsp. japonica	29%	100%
Q6Z2M9	Oryza sativa subsp. japonica	27%	100%
Q9Y6E0	Homo sapiens	26%	100%
Q9ZV15	Arabidopsis thaliana	26%	91%
V5BHW8	Trypanosoma cruzi	25%	84%
V5BLW4	Trypanosoma cruzi	31%	69%