Putative cytochrome b-domain protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cytochrome b-domain protein, putative

Species:

Leishmania major

UniProt:

E9AC79_LEIMA

TriTrypDb:

LmjF.02.0050 , LMJLV39_020005300 , LMJSD75_020005400

Length:

218

Annotations

LeishMANIAdb annotations

This cluster contains a high variety of cytochrome b5 homologs. Although all membrane-anchored, some use the N-terminal [type-III] while others the C-terminal TM helix [type-IV]. The heme domain is assumed to be cytoplasmic.. Localization: ER (by homology) / Mitochondrial outer membrane (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	9
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	9

Expansion

Sequence features

E9AC79

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AC79

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	9
GO:0020037	heme binding	4	9
GO:0043167	ion binding	2	9
GO:0043169	cation binding	3	9
GO:0046872	metal ion binding	4	9
GO:0046906	tetrapyrrole binding	3	9
GO:0097159	organic cyclic compound binding	2	9
GO:1901363	heterocyclic compound binding	2	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	162	164	PF00675	0.444
CLV_NRD_NRD_1	168	170	PF00675	0.530
CLV_NRD_NRD_1	66	68	PF00675	0.267
CLV_NRD_NRD_1	83	85	PF00675	0.415
CLV_PCSK_KEX2_1	82	84	PF00082	0.421
CLV_PCSK_SKI1_1	122	126	PF00082	0.301
CLV_PCSK_SKI1_1	169	173	PF00082	0.521
CLV_PCSK_SKI1_1	41	45	PF00082	0.309
DOC_MAPK_gen_1	15	23	PF00069	0.591
DOC_MAPK_gen_1	67	74	PF00069	0.503
DOC_MAPK_MEF2A_6	201	210	PF00069	0.403
DOC_MAPK_NFAT4_5	201	209	PF00069	0.415
DOC_USP7_MATH_1	93	97	PF00917	0.560
DOC_WW_Pin1_4	109	114	PF00397	0.548
LIG_14-3-3_CanoR_1	163	167	PF00244	0.705
LIG_14-3-3_CanoR_1	83	89	PF00244	0.636
LIG_APCC_ABBA_1	111	116	PF00400	0.548
LIG_BRCT_BRCA1_1	98	102	PF00533	0.431
LIG_FHA_1	110	116	PF00498	0.525
LIG_FHA_1	178	184	PF00498	0.752
LIG_FHA_2	143	149	PF00498	0.431
LIG_LIR_Gen_1	112	119	PF02991	0.513
LIG_LIR_Gen_1	144	155	PF02991	0.542
LIG_LIR_Gen_1	18	28	PF02991	0.558
LIG_LIR_Nem_3	112	117	PF02991	0.524
LIG_LIR_Nem_3	18	23	PF02991	0.485
LIG_LIR_Nem_3	53	59	PF02991	0.501
LIG_LIR_Nem_3	98	104	PF02991	0.455
LIG_PTB_Apo_2	103	110	PF02174	0.501
LIG_PTB_Apo_2	22	29	PF02174	0.560
LIG_PTB_Phospho_1	103	109	PF10480	0.501
LIG_PTB_Phospho_1	22	28	PF10480	0.560
LIG_SH2_SRC	34	37	PF00017	0.523
LIG_SH2_STAT5	153	156	PF00017	0.511
LIG_SH2_STAT5	5	8	PF00017	0.501
LIG_SH2_STAT5	85	88	PF00017	0.624
LIG_UBA3_1	192	201	PF00899	0.637
LIG_WRC_WIRS_1	1	6	PF05994	0.529
MOD_CK1_1	96	102	PF00069	0.431
MOD_CK2_1	142	148	PF00069	0.431
MOD_GlcNHglycan	17	20	PF01048	0.330
MOD_GlcNHglycan	95	98	PF01048	0.358
MOD_GSK3_1	131	138	PF00069	0.560
MOD_GSK3_1	158	165	PF00069	0.674
MOD_GSK3_1	172	179	PF00069	0.689
MOD_GSK3_1	184	191	PF00069	0.677
MOD_LATS_1	13	19	PF00433	0.494
MOD_N-GLC_1	188	193	PF02516	0.468
MOD_N-GLC_1	59	64	PF02516	0.325
MOD_PIKK_1	131	137	PF00454	0.560
MOD_PIKK_1	172	178	PF00454	0.666
MOD_PKA_1	15	21	PF00069	0.494
MOD_PKA_1	169	175	PF00069	0.719
MOD_PKA_2	162	168	PF00069	0.660
MOD_PKA_2	172	178	PF00069	0.623
MOD_PKB_1	82	90	PF00069	0.625
MOD_Plk_1	188	194	PF00069	0.664
MOD_Plk_4	162	168	PF00069	0.660
MOD_Plk_4	188	194	PF00069	0.684
MOD_ProDKin_1	109	115	PF00069	0.548
MOD_SUMO_for_1	185	188	PF00179	0.697
MOD_SUMO_rev_2	157	166	PF00179	0.647
TRG_DiLeu_BaEn_1	162	167	PF01217	0.624
TRG_DiLeu_BaEn_1	188	193	PF01217	0.667
TRG_DiLeu_BaEn_1	198	203	PF01217	0.539
TRG_ENDOCYTIC_2	147	150	PF00928	0.560
TRG_ENDOCYTIC_2	85	88	PF00928	0.624
TRG_ER_diArg_1	82	84	PF00400	0.615

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4I5	Leptomonas seymouri	73%	100%
A0A1X0NJR3	Trypanosomatidae	49%	98%
A0A3S5H4X7	Leishmania donovani	88%	100%
A0A422N6Z2	Trypanosoma rangeli	47%	96%
A4H3A1	Leishmania braziliensis	75%	100%
A4HRK7	Leishmania infantum	89%	100%
E9AJH1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	100%