Metallo-peptidase, Clan MA(E), Family M3

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

metallo-peptidase, Clan MA(E), Family M3

Species:

Leishmania major

UniProt:

E9AC74_LEIMA

TriTrypDb:

LmjF.01.0830 , LMJLV39_010013800 , LMJSD75_010013700

Length:

678

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 27

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 6

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AC74

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AC74

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0019538	protein metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044238	primary metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004175	endopeptidase activity	4	12
GO:0004180	carboxypeptidase activity	5	5
GO:0004222	metalloendopeptidase activity	5	12
GO:0005488	binding	1	12
GO:0008233	peptidase activity	3	12
GO:0008237	metallopeptidase activity	4	12
GO:0008238	exopeptidase activity	4	5
GO:0016787	hydrolase activity	2	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0140096	catalytic activity, acting on a protein	2	12
GO:0008241	peptidyl-dipeptidase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	47	51	PF00656	0.480
CLV_NRD_NRD_1	140	142	PF00675	0.523
CLV_NRD_NRD_1	176	178	PF00675	0.447
CLV_NRD_NRD_1	237	239	PF00675	0.324
CLV_NRD_NRD_1	274	276	PF00675	0.406
CLV_NRD_NRD_1	340	342	PF00675	0.331
CLV_NRD_NRD_1	380	382	PF00675	0.361
CLV_NRD_NRD_1	661	663	PF00675	0.335
CLV_PCSK_KEX2_1	236	238	PF00082	0.324
CLV_PCSK_KEX2_1	379	381	PF00082	0.364
CLV_PCSK_KEX2_1	417	419	PF00082	0.393
CLV_PCSK_PC1ET2_1	417	419	PF00082	0.393
CLV_PCSK_PC7_1	233	239	PF00082	0.384
CLV_PCSK_SKI1_1	161	165	PF00082	0.425
CLV_PCSK_SKI1_1	213	217	PF00082	0.440
CLV_PCSK_SKI1_1	237	241	PF00082	0.384
CLV_PCSK_SKI1_1	268	272	PF00082	0.398
CLV_PCSK_SKI1_1	364	368	PF00082	0.361
CLV_PCSK_SKI1_1	491	495	PF00082	0.426
CLV_PCSK_SKI1_1	667	671	PF00082	0.393
CLV_PCSK_SKI1_1	72	76	PF00082	0.468
DOC_CYCLIN_RxL_1	231	243	PF00134	0.384
DOC_MAPK_gen_1	177	184	PF00069	0.471
DOC_MAPK_gen_1	379	386	PF00069	0.346
DOC_MAPK_gen_1	417	425	PF00069	0.426
DOC_MAPK_RevD_3	260	276	PF00069	0.404
DOC_PP1_RVXF_1	70	76	PF00149	0.420
DOC_PP4_FxxP_1	566	569	PF00568	0.286
DOC_USP7_MATH_1	303	307	PF00917	0.403
DOC_USP7_MATH_1	413	417	PF00917	0.378
DOC_USP7_MATH_1	44	48	PF00917	0.421
DOC_USP7_MATH_1	576	580	PF00917	0.341
DOC_USP7_UBL2_3	152	156	PF12436	0.475
DOC_USP7_UBL2_3	663	667	PF12436	0.426
DOC_WW_Pin1_4	427	432	PF00397	0.275
DOC_WW_Pin1_4	7	12	PF00397	0.415
LIG_14-3-3_CanoR_1	122	126	PF00244	0.514
LIG_14-3-3_CanoR_1	161	166	PF00244	0.465
LIG_14-3-3_CanoR_1	177	183	PF00244	0.409
LIG_14-3-3_CanoR_1	379	387	PF00244	0.346
LIG_Actin_WH2_2	255	270	PF00022	0.393
LIG_Actin_WH2_2	465	482	PF00022	0.372
LIG_APCC_ABBA_1	98	103	PF00400	0.454
LIG_APCC_ABBAyCdc20_2	97	103	PF00400	0.460
LIG_BIR_II_1	1	5	PF00653	0.545
LIG_BRCT_BRCA1_1	592	596	PF00533	0.363
LIG_EH1_1	439	447	PF00400	0.343
LIG_FHA_1	100	106	PF00498	0.474
LIG_FHA_1	177	183	PF00498	0.473
LIG_FHA_1	253	259	PF00498	0.426
LIG_FHA_1	422	428	PF00498	0.411
LIG_FHA_1	542	548	PF00498	0.420
LIG_FHA_1	562	568	PF00498	0.193
LIG_FHA_2	81	87	PF00498	0.492
LIG_Integrin_RGD_1	249	251	PF01839	0.393
LIG_KLC1_Yacidic_2	103	108	PF13176	0.441
LIG_LIR_Apic_2	563	569	PF02991	0.286
LIG_LIR_Gen_1	393	398	PF02991	0.315
LIG_LIR_Gen_1	462	472	PF02991	0.335
LIG_LIR_Gen_1	492	500	PF02991	0.367
LIG_LIR_Gen_1	554	565	PF02991	0.370
LIG_LIR_Gen_1	620	626	PF02991	0.343
LIG_LIR_Nem_3	332	336	PF02991	0.339
LIG_LIR_Nem_3	393	397	PF02991	0.315
LIG_LIR_Nem_3	462	468	PF02991	0.335
LIG_LIR_Nem_3	492	496	PF02991	0.428
LIG_LIR_Nem_3	502	507	PF02991	0.419
LIG_LIR_Nem_3	554	560	PF02991	0.355
LIG_LIR_Nem_3	606	611	PF02991	0.346
LIG_LIR_Nem_3	620	625	PF02991	0.340
LIG_LRP6_Inhibitor_1	110	116	PF00058	0.355
LIG_Pex14_1	331	335	PF04695	0.330
LIG_Pex14_1	422	426	PF04695	0.311
LIG_Pex14_2	596	600	PF04695	0.320
LIG_SH2_CRK	112	116	PF00017	0.564
LIG_SH2_CRK	214	218	PF00017	0.429
LIG_SH2_CRK	440	444	PF00017	0.426
LIG_SH2_CRK	507	511	PF00017	0.426
LIG_SH2_NCK_1	27	31	PF00017	0.462
LIG_SH2_NCK_1	412	416	PF00017	0.393
LIG_SH2_SRC	106	109	PF00017	0.436
LIG_SH2_SRC	14	17	PF00017	0.530
LIG_SH2_SRC	27	30	PF00017	0.385
LIG_SH2_SRC	514	517	PF00017	0.402
LIG_SH2_STAP1	136	140	PF00017	0.387
LIG_SH2_STAP1	214	218	PF00017	0.429
LIG_SH2_STAP1	336	340	PF00017	0.334
LIG_SH2_STAP1	507	511	PF00017	0.380
LIG_SH2_STAP1	604	608	PF00017	0.344
LIG_SH2_STAP1	656	660	PF00017	0.426
LIG_SH2_STAT3	426	429	PF00017	0.300
LIG_SH2_STAT3	609	612	PF00017	0.370
LIG_SH2_STAT5	106	109	PF00017	0.436
LIG_SH2_STAT5	355	358	PF00017	0.367
LIG_SH2_STAT5	368	371	PF00017	0.283
LIG_SH2_STAT5	426	429	PF00017	0.349
LIG_SH2_STAT5	552	555	PF00017	0.371
LIG_SH2_STAT5	609	612	PF00017	0.392
LIG_SH3_1	46	52	PF00018	0.501
LIG_SH3_3	383	389	PF00018	0.333
LIG_SH3_3	46	52	PF00018	0.501
LIG_Sin3_3	293	300	PF02671	0.393
LIG_SUMO_SIM_anti_2	544	549	PF11976	0.402
LIG_SUMO_SIM_par_1	215	222	PF11976	0.455
LIG_SUMO_SIM_par_1	57	62	PF11976	0.450
LIG_TRFH_1	326	330	PF08558	0.387
LIG_TRFH_1	407	411	PF08558	0.311
LIG_TYR_ITIM	438	443	PF00017	0.426
LIG_UBA3_1	475	480	PF00899	0.370
LIG_WRC_WIRS_1	391	396	PF05994	0.370
LIG_WRC_WIRS_1	577	582	PF05994	0.348
LIG_WRC_WIRS_1	597	602	PF05994	0.193
LIG_WW_1	588	591	PF00397	0.333
LIG_WW_3	587	591	PF00397	0.333
MOD_CK1_1	229	235	PF00069	0.398
MOD_CK1_1	459	465	PF00069	0.336
MOD_CK1_1	482	488	PF00069	0.506
MOD_CK1_1	80	86	PF00069	0.490
MOD_CK1_1	99	105	PF00069	0.579
MOD_CK2_1	191	197	PF00069	0.569
MOD_CK2_1	80	86	PF00069	0.425
MOD_Cter_Amidation	273	276	PF01082	0.393
MOD_Cter_Amidation	660	663	PF01082	0.327
MOD_GlcNHglycan	1	4	PF01048	0.563
MOD_GlcNHglycan	254	258	PF01048	0.333
MOD_GlcNHglycan	291	294	PF01048	0.426
MOD_GlcNHglycan	484	487	PF01048	0.488
MOD_GlcNHglycan	592	595	PF01048	0.384
MOD_GlcNHglycan	600	603	PF01048	0.340
MOD_GlcNHglycan	62	66	PF01048	0.579
MOD_GlcNHglycan	79	82	PF01048	0.332
MOD_GSK3_1	456	463	PF00069	0.333
MOD_GSK3_1	480	487	PF00069	0.368
MOD_GSK3_1	50	57	PF00069	0.424
MOD_GSK3_1	642	649	PF00069	0.333
MOD_N-GLC_1	219	224	PF02516	0.405
MOD_N-GLC_1	252	257	PF02516	0.333
MOD_N-GLC_2	77	79	PF02516	0.445
MOD_NEK2_1	226	231	PF00069	0.347
MOD_NEK2_1	240	245	PF00069	0.333
MOD_NEK2_1	397	402	PF00069	0.277
MOD_NEK2_1	479	484	PF00069	0.357
MOD_NEK2_1	54	59	PF00069	0.402
MOD_NEK2_1	596	601	PF00069	0.357
MOD_NEK2_1	61	66	PF00069	0.390
MOD_NEK2_2	374	379	PF00069	0.302
MOD_NEK2_2	421	426	PF00069	0.370
MOD_NEK2_2	96	101	PF00069	0.418
MOD_PIKK_1	219	225	PF00454	0.414
MOD_PIKK_1	495	501	PF00454	0.333
MOD_PKA_1	379	385	PF00069	0.302
MOD_PKA_2	121	127	PF00069	0.496
MOD_PKA_2	176	182	PF00069	0.471
MOD_PKA_2	232	238	PF00069	0.393
MOD_PKA_2	379	385	PF00069	0.337
MOD_PKA_2	413	419	PF00069	0.384
MOD_Plk_1	240	246	PF00069	0.380
MOD_Plk_1	253	259	PF00069	0.418
MOD_Plk_1	459	465	PF00069	0.393
MOD_Plk_1	54	60	PF00069	0.427
MOD_Plk_1	561	567	PF00069	0.367
MOD_Plk_2-3	191	197	PF00069	0.458
MOD_Plk_2-3	20	26	PF00069	0.413
MOD_Plk_2-3	390	396	PF00069	0.393
MOD_Plk_2-3	50	56	PF00069	0.446
MOD_Plk_4	421	427	PF00069	0.426
MOD_Plk_4	451	457	PF00069	0.317
MOD_Plk_4	460	466	PF00069	0.365
MOD_Plk_4	54	60	PF00069	0.464
MOD_ProDKin_1	427	433	PF00069	0.275
MOD_ProDKin_1	7	13	PF00069	0.412
MOD_SUMO_for_1	308	311	PF00179	0.348
MOD_SUMO_rev_2	311	318	PF00179	0.348
MOD_SUMO_rev_2	346	354	PF00179	0.399
TRG_DiLeu_BaEn_1	128	133	PF01217	0.378
TRG_DiLeu_BaEn_1	69	74	PF01217	0.350
TRG_DiLeu_BaEn_2	561	567	PF01217	0.286
TRG_DiLeu_BaEn_4	562	568	PF01217	0.370
TRG_DiLeu_BaLyEn_6	2	7	PF01217	0.494
TRG_DiLeu_BaLyEn_6	222	227	PF01217	0.539
TRG_DiLeu_BaLyEn_6	428	433	PF01217	0.232
TRG_ENDOCYTIC_2	112	115	PF00928	0.409
TRG_ENDOCYTIC_2	214	217	PF00928	0.445
TRG_ENDOCYTIC_2	355	358	PF00928	0.393
TRG_ENDOCYTIC_2	440	443	PF00928	0.426
TRG_ENDOCYTIC_2	507	510	PF00928	0.393
TRG_ENDOCYTIC_2	552	555	PF00928	0.416
TRG_ENDOCYTIC_2	656	659	PF00928	0.333
TRG_ER_diArg_1	230	233	PF00400	0.335
TRG_ER_diArg_1	236	238	PF00400	0.317
TRG_ER_diArg_1	378	381	PF00400	0.386
TRG_ER_diArg_1	70	73	PF00400	0.415
TRG_NLS_MonoExtC_3	340	346	PF00514	0.348
TRG_NLS_MonoExtN_4	266	272	PF00514	0.370
TRG_NLS_MonoExtN_4	339	345	PF00514	0.366
TRG_Pf-PMV_PEXEL_1	237	241	PF00026	0.324
TRG_Pf-PMV_PEXEL_1	615	620	PF00026	0.393

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HT30	Leptomonas seymouri	74%	100%
A0A0N1PBM8	Leptomonas seymouri	26%	99%
A0A0N1PDB7	Leptomonas seymouri	56%	100%
A0A0S4JRG5	Bodo saltans	24%	100%
A0A1X0NTS0	Trypanosomatidae	25%	99%
A0A1X0P0Z5	Trypanosomatidae	24%	98%
A0A3S5H518	Leishmania donovani	92%	100%
A0A3S7WZY5	Leishmania donovani	24%	99%
A0A422MUB5	Trypanosoma rangeli	24%	99%
A0A422P572	Trypanosoma rangeli	23%	99%
A1DMR2	Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181)	24%	85%
A2QWM4	Aspergillus niger (strain CBS 513.88 / FGSC A1513)	25%	85%
A2VDQ5	Bos taurus	27%	96%
A3LUT4	Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)	22%	83%
A4HF12	Leishmania braziliensis	25%	100%
A4HFS7	Leishmania braziliensis	82%	100%
A4HFS8	Leishmania braziliensis	82%	100%
A4HG35	Leishmania braziliensis	85%	100%
A4HG37	Leishmania braziliensis	82%	100%
A4HPW2	Leishmania braziliensis	23%	100%
A4HRR9	Leishmania infantum	92%	100%
A4I291	Leishmania infantum	24%	99%
A4RF25	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	23%	83%
A5DI46	Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)	22%	86%
A6H611	Mus musculus	23%	95%
A6SHZ5	Botryotinia fuckeliana (strain B05.10)	23%	89%
A6ZZI7	Saccharomyces cerevisiae (strain YJM789)	23%	88%
A7E7L8	Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1)	23%	86%
A7TSL2	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	24%	86%
A8N2T3	Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003)	23%	87%
A8QB25	Malassezia globosa (strain ATCC MYA-4612 / CBS 7966)	25%	84%
B0CRC2	Laccaria bicolor (strain S238N-H82 / ATCC MYA-4686)	22%	88%
B0Y7Q2	Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163)	24%	85%
C9ZRP1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	91%
E9ACE8	Leishmania major	97%	100%
E9AJN6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9AYD6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	99%
F4HTQ1	Arabidopsis thaliana	23%	95%
F4KDA5	Arabidopsis thaliana	25%	96%
P0CQ18	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	22%	89%
P0CQ19	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	22%	89%
P0CQ20	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	22%	82%
P0CQ21	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	22%	82%
P24155	Rattus norvegicus	30%	99%
P24171	Escherichia coli (strain K12)	45%	100%
P25375	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	95%
P27236	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	44%	100%
P27237	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	31%	100%
P27298	Escherichia coli (strain K12)	31%	100%
P35999	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	88%
P37932	Schizophyllum commune	22%	87%
P42675	Oryctolagus cuniculus	28%	96%
P42676	Rattus norvegicus	28%	96%
P44573	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	31%	100%
P47788	Sus scrofa	30%	99%
P52888	Homo sapiens	29%	98%
Q01992	Rattus norvegicus	23%	95%
Q02038	Sus scrofa	28%	96%
Q0TXL7	Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173)	25%	86%
Q10415	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	89%
Q1E8M9	Coccidioides immitis (strain RS)	23%	85%
Q1JPJ8	Bos taurus	30%	99%
Q2HFL8	Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970)	24%	87%
Q2UN31	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	25%	85%
Q4PBS8	Ustilago maydis (strain 521 / FGSC 9021)	23%	76%
Q4Q937	Leishmania major	24%	99%
Q4WMU9	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	24%	85%
Q54DD2	Dictyostelium discoideum	26%	100%
Q59RK9	Candida albicans (strain SC5314 / ATCC MYA-2876)	23%	87%
Q5R9V6	Pongo abelii	27%	96%
Q5RF14	Pongo abelii	22%	95%
Q6BJ61	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	23%	85%
Q6CHD6	Yarrowia lipolytica (strain CLIB 122 / E 150)	24%	87%
Q6CVF7	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	24%	87%
Q6FW88	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	23%	89%
Q6VMB4	Leucoagaricus gongylophorus	23%	89%
Q6Y5M5	Pleurotus djamor	23%	86%
Q6Y5M6	Coprinellus disseminatus	23%	88%
Q6Y5M7	Coprinopsis scobicola	22%	88%
Q753X4	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	24%	87%
Q7SDD5	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	24%	84%
Q8C1A5	Mus musculus	30%	99%
Q91YP2	Mus musculus	28%	96%
Q949P2	Arabidopsis thaliana	30%	97%
Q94AM1	Arabidopsis thaliana	31%	86%
Q99797	Homo sapiens	22%	95%
Q9BYT8	Homo sapiens	27%	96%