Alpha/beta-hydrolase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

nicotinamidase, putative

Species:

Leishmania major

UniProt:

E9AC36_LEIMA

TriTrypDb:

LmjF.01.0450 , LMJLV39_010009800 , LMJSD75_010009800

Length:

218

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AC36

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AC36

PDB structure(s): 3r2j_A , 3r2j_B , 3r2j_C , 3r2j_D

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006753	nucleoside phosphate metabolic process	4	2
GO:0006793	phosphorus metabolic process	3	2
GO:0006796	phosphate-containing compound metabolic process	4	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009058	biosynthetic process	2	2
GO:0009117	nucleotide metabolic process	5	2
GO:0009165	nucleotide biosynthetic process	6	2
GO:0009435	NAD biosynthetic process	8	2
GO:0009987	cellular process	1	2
GO:0018130	heterocycle biosynthetic process	4	2
GO:0019359	nicotinamide nucleotide biosynthetic process	7	2
GO:0019362	pyridine nucleotide metabolic process	5	2
GO:0019363	pyridine nucleotide biosynthetic process	6	2
GO:0019438	aromatic compound biosynthetic process	4	2
GO:0019637	organophosphate metabolic process	3	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034654	nucleobase-containing compound biosynthetic process	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044249	cellular biosynthetic process	3	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0044281	small molecule metabolic process	2	2
GO:0046483	heterocycle metabolic process	3	2
GO:0046496	nicotinamide nucleotide metabolic process	6	2
GO:0055086	nucleobase-containing small molecule metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0072524	pyridine-containing compound metabolic process	4	2
GO:0072525	pyridine-containing compound biosynthetic process	5	2
GO:0090407	organophosphate biosynthetic process	4	2
GO:1901293	nucleoside phosphate biosynthetic process	5	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901362	organic cyclic compound biosynthetic process	4	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901566	organonitrogen compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	6
GO:0008936	nicotinamidase activity	5	2
GO:0016787	hydrolase activity	2	6
GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	3	2
GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	4	2
GO:0005488	binding	1	1
GO:0043167	ion binding	2	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	165	169	PF00656	0.478
CLV_NRD_NRD_1	115	117	PF00675	0.354
CLV_NRD_NRD_1	149	151	PF00675	0.296
CLV_NRD_NRD_1	169	171	PF00675	0.155
CLV_PCSK_KEX2_1	149	151	PF00082	0.296
CLV_PCSK_SKI1_1	117	121	PF00082	0.280
CLV_PCSK_SKI1_1	149	153	PF00082	0.296
DEG_SCF_FBW7_1	38	43	PF00400	0.412
DEG_SCF_FBW7_1	9	16	PF00400	0.489
DOC_CKS1_1	37	42	PF01111	0.412
DOC_MAPK_DCC_7	10	19	PF00069	0.526
DOC_MAPK_gen_1	170	178	PF00069	0.534
DOC_MAPK_HePTP_8	7	19	PF00069	0.442
DOC_MAPK_MEF2A_6	10	19	PF00069	0.526
DOC_PP4_FxxP_1	37	40	PF00568	0.478
DOC_USP7_MATH_1	40	44	PF00917	0.503
DOC_USP7_MATH_1	58	62	PF00917	0.569
DOC_WW_Pin1_4	36	41	PF00397	0.501
DOC_WW_Pin1_4	9	14	PF00397	0.590
LIG_14-3-3_CanoR_1	116	126	PF00244	0.548
LIG_CSL_BTD_1	88	91	PF09270	0.461
LIG_FHA_1	106	112	PF00498	0.560
LIG_FHA_1	14	20	PF00498	0.562
LIG_FHA_1	181	187	PF00498	0.492
LIG_FHA_1	22	28	PF00498	0.429
LIG_FHA_1	4	10	PF00498	0.558
LIG_FHA_2	163	169	PF00498	0.478
LIG_FHA_2	3	9	PF00498	0.664
LIG_LIR_Apic_2	36	40	PF02991	0.478
LIG_LIR_Gen_1	124	130	PF02991	0.492
LIG_LIR_Gen_1	159	167	PF02991	0.530
LIG_LIR_Nem_3	124	129	PF02991	0.475
LIG_LIR_Nem_3	159	163	PF02991	0.477
LIG_NRBOX	138	144	PF00104	0.496
LIG_Pex14_2	160	164	PF04695	0.548
LIG_SH2_STAP1	126	130	PF00017	0.513
LIG_SH2_STAP1	158	162	PF00017	0.513
LIG_SH3_3	85	91	PF00018	0.469
LIG_SUMO_SIM_anti_2	24	32	PF11976	0.322
LIG_SUMO_SIM_par_1	175	181	PF11976	0.466
LIG_SUMO_SIM_par_1	202	209	PF11976	0.554
LIG_SUMO_SIM_par_1	24	32	PF11976	0.323
MOD_CK1_1	22	28	PF00069	0.447
MOD_CK1_1	81	87	PF00069	0.542
MOD_GlcNHglycan	126	129	PF01048	0.293
MOD_GlcNHglycan	191	194	PF01048	0.314
MOD_GlcNHglycan	208	211	PF01048	0.421
MOD_GlcNHglycan	97	100	PF01048	0.327
MOD_GSK3_1	17	24	PF00069	0.480
MOD_GSK3_1	36	43	PF00069	0.430
MOD_GSK3_1	9	16	PF00069	0.657
MOD_N-GLC_1	22	27	PF02516	0.461
MOD_N-GLC_2	78	80	PF02516	0.354
MOD_NEK2_1	142	147	PF00069	0.559
MOD_NEK2_1	17	22	PF00069	0.519
MOD_NEK2_1	205	210	PF00069	0.523
MOD_NEK2_2	40	45	PF00069	0.503
MOD_PIKK_1	117	123	PF00454	0.467
MOD_PIKK_1	58	64	PF00454	0.573
MOD_PIKK_1	68	74	PF00454	0.531
MOD_Plk_4	78	84	PF00069	0.548
MOD_ProDKin_1	36	42	PF00069	0.501
MOD_ProDKin_1	9	15	PF00069	0.591
MOD_SUMO_rev_2	192	201	PF00179	0.558
TRG_ENDOCYTIC_2	126	129	PF00928	0.464
TRG_ER_diArg_1	149	151	PF00400	0.499

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P300	Leptomonas seymouri	62%	100%
A0A1X0P2N6	Trypanosomatidae	46%	80%
A0A3S5H4V3	Leishmania donovani	94%	100%
A0A422NY51	Trypanosoma rangeli	46%	90%
A4H371	Leishmania braziliensis	79%	98%
A4HRG8	Leishmania infantum	94%	100%
C9ZXJ1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	100%
E9AJD2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	80%	100%
I6XD65	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	43%	100%
P21369	Escherichia coli (strain K12)	38%	100%
P53184	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	100%
Q9USS0	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	99%
V5BLL8	Trypanosoma cruzi	46%	98%