Leishmanolysin

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Leishmanolysin

Gene product:

hypothetical protein

Species:

Leishmania mexicana

UniProt:

E8NHL1_LEIMU

TriTrypDb:

LmxM.10.0465a

Length:

251

Annotations

LeishMANIAdb annotations

Publication identifier(s): 31356625
GPI-anchored cell surface protease. Broad-spectrum ectoenzyme involved in pathogenesis. Heavily expanded family in all parazitic species.. Localization: Cell surface (experimental)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 13, no: 23
NetGPI	no	yes: 0, no: 36

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	37
GO:0110165	cellular anatomical entity	1	37

Expansion

Sequence features

E8NHL1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E8NHL1

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	37
GO:0006807	nitrogen compound metabolic process	2	37
GO:0007155	cell adhesion	2	37
GO:0008152	metabolic process	1	37
GO:0009987	cellular process	1	37
GO:0019538	protein metabolic process	3	37
GO:0043170	macromolecule metabolic process	3	37
GO:0044238	primary metabolic process	2	37
GO:0071704	organic substance metabolic process	2	37
GO:1901564	organonitrogen compound metabolic process	3	37

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	37
GO:0004175	endopeptidase activity	4	37
GO:0004222	metalloendopeptidase activity	5	37
GO:0005488	binding	1	37
GO:0008233	peptidase activity	3	37
GO:0008237	metallopeptidase activity	4	37
GO:0016787	hydrolase activity	2	37
GO:0043167	ion binding	2	37
GO:0043169	cation binding	3	37
GO:0046872	metal ion binding	4	37
GO:0140096	catalytic activity, acting on a protein	2	37

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_SKI1_1	228	232	PF00082	0.530
CLV_PCSK_SKI1_1	92	96	PF00082	0.569
DEG_Nend_UBRbox_1	1	3	PF02207	0.392
DEG_SPOP_SBC_1	130	134	PF00917	0.382
DOC_CKS1_1	241	246	PF01111	0.652
DOC_MAPK_gen_1	122	130	PF00069	0.306
DOC_PP2B_LxvP_1	176	179	PF13499	0.331
DOC_PP4_FxxP_1	26	29	PF00568	0.300
DOC_USP7_MATH_1	179	183	PF00917	0.360
DOC_USP7_UBL2_3	224	228	PF12436	0.392
DOC_WW_Pin1_4	240	245	PF00397	0.652
LIG_AP2alpha_1	104	108	PF02296	0.220
LIG_APCC_ABBA_1	191	196	PF00400	0.369
LIG_BRCT_BRCA1_1	104	108	PF00533	0.381
LIG_FHA_1	188	194	PF00498	0.338
LIG_FHA_1	32	38	PF00498	0.305
LIG_FHA_1	59	65	PF00498	0.364
LIG_FHA_1	89	95	PF00498	0.360
LIG_FHA_2	105	111	PF00498	0.349
LIG_FHA_2	36	42	PF00498	0.348
LIG_LIR_Apic_2	243	248	PF02991	0.708
LIG_LIR_Apic_2	25	29	PF02991	0.312
LIG_LIR_Gen_1	106	115	PF02991	0.309
LIG_LIR_Gen_1	148	156	PF02991	0.385
LIG_LIR_Gen_1	84	94	PF02991	0.315
LIG_LIR_Nem_3	106	112	PF02991	0.314
LIG_LIR_Nem_3	148	154	PF02991	0.385
LIG_LIR_Nem_3	40	45	PF02991	0.314
LIG_LIR_Nem_3	84	89	PF02991	0.307
LIG_Pex14_1	233	237	PF04695	0.405
LIG_Pex14_2	104	108	PF04695	0.393
LIG_PTB_Apo_2	36	43	PF02174	0.359
LIG_SH2_CRK	186	190	PF00017	0.258
LIG_SH2_CRK	245	249	PF00017	0.713
LIG_SH2_CRK	86	90	PF00017	0.283
LIG_SH2_SRC	151	154	PF00017	0.283
LIG_SH2_STAP1	86	90	PF00017	0.366
LIG_SH2_STAT5	151	154	PF00017	0.398
LIG_SH2_STAT5	186	189	PF00017	0.275
LIG_SH2_STAT5	45	48	PF00017	0.310
LIG_SH3_3	238	244	PF00018	0.530
LIG_TRAF2_1	50	53	PF00917	0.220
LIG_TYR_ITIM	149	154	PF00017	0.420
MOD_CK1_1	129	135	PF00069	0.385
MOD_CK1_1	162	168	PF00069	0.426
MOD_CK1_1	48	54	PF00069	0.456
MOD_CK2_1	104	110	PF00069	0.353
MOD_CK2_1	202	208	PF00069	0.372
MOD_DYRK1A_RPxSP_1	240	244	PF00069	0.455
MOD_GlcNHglycan	161	164	PF01048	0.422
MOD_GlcNHglycan	181	184	PF01048	0.376
MOD_GSK3_1	126	133	PF00069	0.383
MOD_GSK3_1	31	38	PF00069	0.398
MOD_GSK3_1	88	95	PF00069	0.477
MOD_N-GLC_1	126	131	PF02516	0.379
MOD_N-GLC_1	228	233	PF02516	0.455
MOD_NEK2_1	104	109	PF00069	0.381
MOD_NEK2_1	126	131	PF00069	0.404
MOD_Plk_1	126	132	PF00069	0.425
MOD_Plk_1	51	57	PF00069	0.428
MOD_Plk_4	104	110	PF00069	0.425
MOD_Plk_4	126	132	PF00069	0.410
MOD_Plk_4	51	57	PF00069	0.483
MOD_ProDKin_1	240	246	PF00069	0.651
MOD_SUMO_rev_2	20	29	PF00179	0.474
TRG_ENDOCYTIC_2	151	154	PF00928	0.475
TRG_ENDOCYTIC_2	186	189	PF00928	0.286
TRG_ENDOCYTIC_2	86	89	PF00928	0.374
TRG_ER_diArg_1	239	242	PF00400	0.623

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2A9	Leptomonas seymouri	59%	69%
A0A0N1IFV2	Leptomonas seymouri	42%	99%
A0A1X0NDC6	Trypanosomatidae	29%	67%
A0A1X0NDI6	Trypanosomatidae	36%	83%
A0A1X0NDK0	Trypanosomatidae	29%	73%
A0A1X0NFB6	Trypanosomatidae	28%	75%
A0A1X0NFI2	Trypanosomatidae	31%	73%
A0A1X0NFK9	Trypanosomatidae	39%	100%
A0A1X0NPU7	Trypanosomatidae	42%	77%
A0A1X0NQB3	Trypanosomatidae	33%	100%
A0A1X0NX09	Trypanosomatidae	32%	74%
A0A1X0NXI6	Trypanosomatidae	32%	100%
A0A1X0NXI9	Trypanosomatidae	36%	76%
A0A1X0NXK4	Trypanosomatidae	29%	69%
A0A1X0NXS5	Trypanosomatidae	31%	100%
A0A1X0NXY0	Trypanosomatidae	27%	73%
A0A1X0NZ76	Trypanosomatidae	28%	71%
A0A1X0P1G3	Trypanosomatidae	29%	68%
A0A1X0P6E8	Trypanosomatidae	38%	69%
A0A1X0P868	Trypanosomatidae	33%	80%
A0A3R7JUH9	Trypanosoma rangeli	48%	73%
A0A3R7K754	Trypanosoma rangeli	39%	67%
A0A3R7R4F7	Trypanosoma rangeli	42%	74%
A0A422MR94	Trypanosoma rangeli	49%	86%
A0A422MUP1	Trypanosoma rangeli	47%	73%
A0A422NLZ8	Trypanosoma rangeli	30%	100%
A0A422NN26	Trypanosoma rangeli	38%	68%
A4H6D2	Leishmania braziliensis	65%	74%
A4H6E7	Leishmania braziliensis	73%	100%
V5ABN5	Trypanosoma cruzi	33%	96%
V5ALP9	Trypanosoma cruzi	40%	93%
V5APB3	Trypanosoma cruzi	38%	78%
V5AZB2	Trypanosoma cruzi	36%	90%
V5B972	Trypanosoma cruzi	37%	100%
V5CHU5	Trypanosoma cruzi	47%	78%