WGS CADB00000000 data, contig 27

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

sphingolipid delta 4 desaturase, putative

Species:

Leishmania mexicana

UniProt:

E8NHH2_LEIMU

TriTrypDb:

LmxM.26.1670 , LmxM.26.1690

Length:

319

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	16
GO:0110165	cellular anatomical entity	1	16

Expansion

Sequence features

E8NHH2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E8NHH2

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	16
GO:0006643	membrane lipid metabolic process	4	14
GO:0006665	sphingolipid metabolic process	4	14
GO:0006807	nitrogen compound metabolic process	2	14
GO:0008152	metabolic process	1	16
GO:0008610	lipid biosynthetic process	4	14
GO:0009058	biosynthetic process	2	14
GO:0009987	cellular process	1	14
GO:0030148	sphingolipid biosynthetic process	5	14
GO:0044237	cellular metabolic process	2	14
GO:0044238	primary metabolic process	2	16
GO:0044249	cellular biosynthetic process	3	14
GO:0044255	cellular lipid metabolic process	3	14
GO:0046467	membrane lipid biosynthetic process	4	14
GO:0071704	organic substance metabolic process	2	16
GO:1901564	organonitrogen compound metabolic process	3	14
GO:1901566	organonitrogen compound biosynthetic process	4	14
GO:1901576	organic substance biosynthetic process	3	14
GO:0006672	ceramide metabolic process	4	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043603	amide metabolic process	3	2
GO:0043604	amide biosynthetic process	4	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0046513	ceramide biosynthetic process	5	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	14
GO:0016491	oxidoreductase activity	2	14
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	14
GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water	4	14
GO:0042284	sphingolipid delta-4 desaturase activity	5	14

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	112	116	PF00656	0.303
CLV_PCSK_SKI1_1	133	137	PF00082	0.300
CLV_PCSK_SKI1_1	152	156	PF00082	0.262
CLV_PCSK_SKI1_1	21	25	PF00082	0.252
DEG_COP1_1	112	121	PF00400	0.186
DEG_Nend_Nbox_1	1	3	PF02207	0.551
DOC_CKS1_1	22	27	PF01111	0.340
DOC_CYCLIN_yCln2_LP_2	22	28	PF00134	0.380
DOC_CYCLIN_yCln2_LP_2	88	94	PF00134	0.324
DOC_MAPK_gen_1	152	161	PF00069	0.470
DOC_MAPK_MEF2A_6	21	29	PF00069	0.296
DOC_MAPK_MEF2A_6	38	47	PF00069	0.225
DOC_PP1_RVXF_1	105	112	PF00149	0.291
DOC_PP1_RVXF_1	150	157	PF00149	0.475
DOC_PP2B_LxvP_1	88	91	PF13499	0.324
DOC_PP4_FxxP_1	73	76	PF00568	0.510
DOC_WW_Pin1_4	21	26	PF00397	0.340
LIG_14-3-3_CanoR_1	125	131	PF00244	0.315
LIG_Actin_WH2_2	134	149	PF00022	0.383
LIG_AP2alpha_2	16	18	PF02296	0.519
LIG_BRCT_BRCA1_1	204	208	PF00533	0.500
LIG_CSL_BTD_1	17	20	PF09270	0.383
LIG_FHA_1	136	142	PF00498	0.306
LIG_FHA_1	22	28	PF00498	0.314
LIG_FHA_1	42	48	PF00498	0.346
LIG_FHA_1	66	72	PF00498	0.457
LIG_FHA_2	118	124	PF00498	0.262
LIG_FXI_DFP_1	238	242	PF00024	0.242
LIG_KLC1_Yacidic_2	258	262	PF13176	0.586
LIG_LIR_Apic_2	218	224	PF02991	0.451
LIG_LIR_Gen_1	138	146	PF02991	0.296
LIG_LIR_Gen_1	153	164	PF02991	0.435
LIG_LIR_Gen_1	24	33	PF02991	0.302
LIG_LIR_Gen_1	59	67	PF02991	0.353
LIG_LIR_Gen_1	80	90	PF02991	0.317
LIG_LIR_Nem_3	138	142	PF02991	0.293
LIG_LIR_Nem_3	153	159	PF02991	0.435
LIG_LIR_Nem_3	194	199	PF02991	0.340
LIG_LIR_Nem_3	217	222	PF02991	0.442
LIG_LIR_Nem_3	24	29	PF02991	0.258
LIG_LIR_Nem_3	240	244	PF02991	0.442
LIG_LIR_Nem_3	258	263	PF02991	0.493
LIG_LIR_Nem_3	39	45	PF02991	0.203
LIG_LIR_Nem_3	59	64	PF02991	0.112
LIG_LIR_Nem_3	80	85	PF02991	0.333
LIG_NRBOX	183	189	PF00104	0.415
LIG_Pex14_1	181	185	PF04695	0.291
LIG_Pex14_1	230	234	PF04695	0.475
LIG_Pex14_2	135	139	PF04695	0.303
LIG_PTB_Apo_2	228	235	PF02174	0.533
LIG_PTB_Apo_2	76	83	PF02174	0.475
LIG_PTB_Phospho_1	228	234	PF10480	0.533
LIG_PTB_Phospho_1	76	82	PF10480	0.491
LIG_SH2_CRK	222	226	PF00017	0.442
LIG_SH2_PTP2	260	263	PF00017	0.583
LIG_SH2_STAP1	82	86	PF00017	0.340
LIG_SH2_STAP1	94	98	PF00017	0.315
LIG_SH2_STAT3	164	167	PF00017	0.340
LIG_SH2_STAT5	108	111	PF00017	0.251
LIG_SH2_STAT5	126	129	PF00017	0.251
LIG_SH2_STAT5	164	167	PF00017	0.333
LIG_SH2_STAT5	185	188	PF00017	0.323
LIG_SH2_STAT5	260	263	PF00017	0.579
LIG_SH2_STAT5	32	35	PF00017	0.278
LIG_SH2_STAT5	50	53	PF00017	0.112
LIG_SH3_3	14	20	PF00018	0.526
LIG_SH3_3	239	245	PF00018	0.451
LIG_SH3_3	87	93	PF00018	0.322
LIG_SUMO_SIM_anti_2	44	49	PF11976	0.440
LIG_TRFH_1	144	148	PF08558	0.352
LIG_TYR_ITIM	183	188	PF00017	0.362
LIG_WRC_WIRS_1	136	141	PF05994	0.307
LIG_WRC_WIRS_1	188	193	PF05994	0.415
MOD_CK1_1	283	289	PF00069	0.563
MOD_CK2_1	117	123	PF00069	0.262
MOD_CMANNOS	178	181	PF00535	0.440
MOD_CMANNOS	227	230	PF00535	0.262
MOD_GSK3_1	146	153	PF00069	0.533
MOD_GSK3_1	187	194	PF00069	0.380
MOD_GSK3_1	52	59	PF00069	0.301
MOD_NEK2_1	135	140	PF00069	0.312
MOD_NEK2_1	187	192	PF00069	0.302
MOD_NEK2_1	208	213	PF00069	0.414
MOD_NEK2_1	263	268	PF00069	0.562
MOD_NEK2_1	277	282	PF00069	0.422
MOD_NEK2_1	97	102	PF00069	0.294
MOD_PKA_2	146	152	PF00069	0.475
MOD_Plk_1	278	284	PF00069	0.513
MOD_Plk_2-3	117	123	PF00069	0.186
MOD_Plk_4	117	123	PF00069	0.177
MOD_Plk_4	146	152	PF00069	0.475
MOD_Plk_4	187	193	PF00069	0.330
MOD_Plk_4	41	47	PF00069	0.269
MOD_ProDKin_1	21	27	PF00069	0.340
MOD_SUMO_for_1	154	157	PF00179	0.533
MOD_SUMO_rev_2	7	12	PF00179	0.513
TRG_DiLeu_BaEn_4	7	13	PF01217	0.400
TRG_DiLeu_BaLyEn_6	179	184	PF01217	0.362
TRG_ENDOCYTIC_2	185	188	PF00928	0.307
TRG_ENDOCYTIC_2	222	225	PF00928	0.442
TRG_ENDOCYTIC_2	260	263	PF00928	0.489
TRG_ENDOCYTIC_2	78	81	PF00928	0.483
TRG_ENDOCYTIC_2	82	85	PF00928	0.342
TRG_ENDOCYTIC_2	94	97	PF00928	0.315
TRG_Pf-PMV_PEXEL_1	107	112	PF00026	0.490

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P225	Leptomonas seymouri	83%	90%
A0A0S4IWE2	Bodo saltans	52%	96%
A0A0S4IZS1	Bodo saltans	52%	93%
A0A1X0NG21	Trypanosomatidae	69%	92%
A0A1X0P7H0	Trypanosomatidae	69%	92%
A0A3S5H7G4	Leishmania donovani	94%	98%
A0A3S7WZX6	Leishmania donovani	94%	95%
A4HD58	Leishmania braziliensis	85%	100%
A4I2A2	Leishmania infantum	95%	88%
C4R613	Komagataella phaffii (strain GS115 / ATCC 20864)	41%	89%
C9ZQX1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	56%	92%
E9AYE9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	100%	95%
G5EC63	Caenorhabditis elegans	41%	88%
O09005	Mus musculus	42%	99%
O15121	Homo sapiens	44%	99%
O44186	Caenorhabditis elegans	41%	89%
O59715	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	45%	88%
Q0II71	Bos taurus	44%	99%
Q3ZBY7	Bos taurus	43%	99%
Q4Q923	Leishmania major	94%	100%
Q4Q925	Leishmania major	94%	100%
Q564G3	Rattus norvegicus	42%	99%
Q5AJX2	Candida albicans (strain SC5314 / ATCC MYA-2876)	45%	86%
Q5F3C1	Gallus gallus	41%	99%
Q5RE51	Pongo abelii	43%	99%
Q5XIF5	Rattus norvegicus	44%	99%
Q68FB8	Xenopus tropicalis	43%	99%
Q6H5U3	Oryza sativa subsp. japonica	43%	97%
Q6QHC5	Homo sapiens	44%	99%
Q6UQ04	Microtus fortis	42%	99%
Q8R2F2	Mus musculus	42%	99%
Q94515	Drosophila melanogaster	44%	99%
Q9ZPH4	Arabidopsis thaliana	42%	96%
V5DBA3	Trypanosoma cruzi	69%	92%