Probable citrate synthase, mitochondrial

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

citrate synthase, putative

Species:

Leishmania major

UniProt:

CISY_LEIMA

TriTrypDb:

LmjF.18.0680 , LMJLV39_180012400 , LMJSD75_180012300

Length:

470

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	yes	yes: 1
Forrest at al. (metacyclic)	no	yes: 1
Forrest at al. (procyclic)	no	yes: 1
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 16

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	3
GO:0005759	mitochondrial matrix	5	5
GO:0020023	kinetoplast	2	3
GO:0031974	membrane-enclosed lumen	2	5
GO:0043226	organelle	2	3
GO:0043227	membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043231	intracellular membrane-bounded organelle	4	3
GO:0043233	organelle lumen	3	5
GO:0070013	intracellular organelle lumen	4	5
GO:0110165	cellular anatomical entity	1	5

Expansion

Sequence features

Q4QDX3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QDX3

Function

Biological processes
Term	Name	Level	Count
GO:0005975	carbohydrate metabolic process	3	3
GO:0006082	organic acid metabolic process	3	2
GO:0006099	tricarboxylic acid cycle	3	5
GO:0006101	citrate metabolic process	7	2
GO:0008152	metabolic process	1	5
GO:0009987	cellular process	1	2
GO:0019752	carboxylic acid metabolic process	5	2
GO:0043436	oxoacid metabolic process	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	5
GO:0044281	small molecule metabolic process	2	2
GO:0071704	organic substance metabolic process	2	3
GO:0072350	tricarboxylic acid metabolic process	6	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	16
GO:0004108	citrate (Si)-synthase activity	6	3
GO:0016740	transferase activity	2	16
GO:0016746	acyltransferase activity	3	16
GO:0036440	citrate synthase activity	5	5
GO:0046912	acyltransferase activity, acyl groups converted into alkyl on transfer	4	16

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	135	137	PF00675	0.333
CLV_NRD_NRD_1	31	33	PF00675	0.607
CLV_NRD_NRD_1	354	356	PF00675	0.409
CLV_NRD_NRD_1	43	45	PF00675	0.505
CLV_PCSK_KEX2_1	135	137	PF00082	0.333
CLV_PCSK_KEX2_1	31	33	PF00082	0.565
CLV_PCSK_SKI1_1	454	458	PF00082	0.468
CLV_PCSK_SKI1_1	65	69	PF00082	0.319
CLV_PCSK_SKI1_1	86	90	PF00082	0.319
DEG_Nend_UBRbox_1	1	4	PF02207	0.607
DEG_SPOP_SBC_1	268	272	PF00917	0.609
DOC_ANK_TNKS_1	313	320	PF00023	0.605
DOC_CYCLIN_yCln2_LP_2	278	284	PF00134	0.576
DOC_MAPK_gen_1	214	222	PF00069	0.566
DOC_PIKK_1	35	43	PF02985	0.514
DOC_PP2B_LxvP_1	222	225	PF13499	0.609
DOC_USP7_MATH_1	168	172	PF00917	0.555
DOC_USP7_MATH_1	181	185	PF00917	0.527
DOC_USP7_MATH_1	223	227	PF00917	0.575
DOC_USP7_UBL2_3	183	187	PF12436	0.566
DOC_USP7_UBL2_3	34	38	PF12436	0.517
DOC_USP7_UBL2_3	393	397	PF12436	0.523
DOC_WW_Pin1_4	201	206	PF00397	0.544
LIG_14-3-3_CanoR_1	16	25	PF00244	0.652
LIG_14-3-3_CanoR_1	252	258	PF00244	0.562
LIG_14-3-3_CanoR_1	360	367	PF00244	0.541
LIG_14-3-3_CanoR_1	5	9	PF00244	0.531
LIG_Actin_WH2_2	1	18	PF00022	0.511
LIG_APCC_ABBA_1	255	260	PF00400	0.519
LIG_APCC_ABBA_1	320	325	PF00400	0.558
LIG_deltaCOP1_diTrp_1	229	236	PF00928	0.521
LIG_FHA_1	115	121	PF00498	0.547
LIG_FHA_1	202	208	PF00498	0.545
LIG_FHA_1	268	274	PF00498	0.521
LIG_FHA_1	334	340	PF00498	0.558
LIG_FHA_1	456	462	PF00498	0.587
LIG_FHA_1	66	72	PF00498	0.530
LIG_FHA_2	186	192	PF00498	0.610
LIG_FHA_2	87	93	PF00498	0.520
LIG_GBD_Chelix_1	380	388	PF00786	0.303
LIG_IBAR_NPY_1	400	402	PF08397	0.609
LIG_LIR_Gen_1	171	180	PF02991	0.582
LIG_LIR_Gen_1	245	255	PF02991	0.493
LIG_LIR_Gen_1	279	289	PF02991	0.533
LIG_LIR_Gen_1	336	345	PF02991	0.519
LIG_LIR_Gen_1	383	392	PF02991	0.517
LIG_LIR_Nem_3	109	115	PF02991	0.575
LIG_LIR_Nem_3	171	175	PF02991	0.574
LIG_LIR_Nem_3	188	192	PF02991	0.455
LIG_LIR_Nem_3	279	285	PF02991	0.519
LIG_LIR_Nem_3	336	340	PF02991	0.519
LIG_LIR_Nem_3	375	381	PF02991	0.519
LIG_LIR_Nem_3	420	426	PF02991	0.489
LIG_LYPXL_yS_3	402	405	PF13949	0.609
LIG_NRBOX	304	310	PF00104	0.597
LIG_PCNA_yPIPBox_3	301	314	PF02747	0.540
LIG_Pex14_1	232	236	PF04695	0.530
LIG_PTB_Apo_2	379	386	PF02174	0.609
LIG_PTB_Phospho_1	379	385	PF10480	0.609
LIG_Rb_LxCxE_1	89	109	PF01857	0.566
LIG_SH2_CRK	424	428	PF00017	0.482
LIG_SH2_SRC	72	75	PF00017	0.575
LIG_SH2_STAP1	172	176	PF00017	0.612
LIG_SH2_STAP1	335	339	PF00017	0.605
LIG_SH2_STAP1	424	428	PF00017	0.482
LIG_SH2_STAT3	361	364	PF00017	0.562
LIG_SH2_STAT5	254	257	PF00017	0.519
LIG_SH2_STAT5	282	285	PF00017	0.519
LIG_SH2_STAT5	307	310	PF00017	0.519
LIG_SH2_STAT5	335	338	PF00017	0.605
LIG_SH2_STAT5	361	364	PF00017	0.528
LIG_SH2_STAT5	424	427	PF00017	0.500
LIG_SH2_STAT5	465	468	PF00017	0.603
LIG_SH3_3	68	74	PF00018	0.551
LIG_SH3_3	95	101	PF00018	0.575
LIG_SUMO_SIM_par_1	165	171	PF11976	0.615
LIG_TRAF2_1	121	124	PF00917	0.644
LIG_TRAF2_1	300	303	PF00917	0.519
LIG_TRAF2_1	310	313	PF00917	0.519
LIG_UBA3_1	308	316	PF00899	0.363
LIG_UBA3_1	387	393	PF00899	0.338
MOD_CK1_1	267	273	PF00069	0.380
MOD_CK1_1	276	282	PF00069	0.339
MOD_CK1_1	333	339	PF00069	0.275
MOD_CK1_1	455	461	PF00069	0.484
MOD_CK2_1	185	191	PF00069	0.488
MOD_CK2_1	307	313	PF00069	0.428
MOD_CK2_1	86	92	PF00069	0.375
MOD_GlcNHglycan	19	22	PF01048	0.535
MOD_GlcNHglycan	266	269	PF01048	0.371
MOD_GlcNHglycan	275	278	PF01048	0.360
MOD_GlcNHglycan	285	288	PF01048	0.402
MOD_GlcNHglycan	341	344	PF01048	0.488
MOD_GlcNHglycan	408	411	PF01048	0.368
MOD_GSK3_1	181	188	PF00069	0.407
MOD_GSK3_1	264	271	PF00069	0.367
MOD_N-GLC_1	264	269	PF02516	0.363
MOD_N-GLC_1	333	338	PF02516	0.483
MOD_N-GLC_2	366	368	PF02516	0.483
MOD_NEK2_1	17	22	PF00069	0.565
MOD_NEK2_1	253	258	PF00069	0.408
MOD_NEK2_1	273	278	PF00069	0.210
MOD_NEK2_1	283	288	PF00069	0.370
MOD_NEK2_1	339	344	PF00069	0.488
MOD_NEK2_1	380	385	PF00069	0.388
MOD_NEK2_1	428	433	PF00069	0.368
MOD_PK_1	452	458	PF00069	0.497
MOD_PKA_2	213	219	PF00069	0.397
MOD_PKA_2	359	365	PF00069	0.394
MOD_PKA_2	4	10	PF00069	0.665
MOD_PKB_1	84	92	PF00069	0.383
MOD_Plk_1	194	200	PF00069	0.385
MOD_Plk_1	333	339	PF00069	0.297
MOD_Plk_1	53	59	PF00069	0.500
MOD_Plk_2-3	119	125	PF00069	0.428
MOD_Plk_4	185	191	PF00069	0.493
MOD_Plk_4	269	275	PF00069	0.365
MOD_Plk_4	380	386	PF00069	0.371
MOD_Plk_4	4	10	PF00069	0.615
MOD_Plk_4	422	428	PF00069	0.324
MOD_Plk_4	72	78	PF00069	0.390
MOD_Plk_4	86	92	PF00069	0.366
MOD_ProDKin_1	201	207	PF00069	0.398
MOD_SUMO_rev_2	35	40	PF00179	0.564
TRG_ENDOCYTIC_2	172	175	PF00928	0.488
TRG_ENDOCYTIC_2	254	257	PF00928	0.363
TRG_ENDOCYTIC_2	282	285	PF00928	0.383
TRG_ENDOCYTIC_2	385	388	PF00928	0.377
TRG_ENDOCYTIC_2	402	405	PF00928	0.470
TRG_ENDOCYTIC_2	423	426	PF00928	0.370
TRG_ENDOCYTIC_2	61	64	PF00928	0.471
TRG_ER_diArg_1	30	32	PF00400	0.579
TRG_NES_CRM1_1	105	119	PF08389	0.488
TRG_NLS_Bipartite_1	31	48	PF00514	0.606
TRG_Pf-PMV_PEXEL_1	127	132	PF00026	0.483

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I014	Leptomonas seymouri	81%	100%
A0A0S3QTD0	Thermosulfidibacter takaii (strain DSM 17441 / JCM 13301 / NBRC 103674 / ABI70S6)	50%	100%
A0A1X0P7W7	Trypanosomatidae	65%	100%
A0A3Q8IB05	Leishmania donovani	94%	100%
A0A3Q8IB38	Leishmania donovani	95%	100%
A0A422P1U5	Trypanosoma rangeli	66%	100%
A4H9H8	Leishmania braziliensis	85%	100%
A4H9H9	Leishmania braziliensis	84%	100%
A4HXU4	Leishmania infantum	95%	100%
A4HXU5	Leishmania infantum	95%	100%
B0YD89	Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163)	49%	100%
C7C435	Fusarium solani	50%	100%
C7C436	Gibberella moniliformis	47%	100%
C9ZZW4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	97%
D4GS06	Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)	28%	100%
E9ARK6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9ARK7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
H8F0D7	Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman)	25%	100%
I6Y9Q3	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	25%	100%
O00098	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	56%	99%
O33915	Rhizobium meliloti (strain 1021)	30%	100%
O34002	Antarctic bacterium DS2-3R	26%	100%
O68883	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	28%	100%
O75390	Homo sapiens	55%	100%
O80433	Daucus carota	55%	100%
P00889	Sus scrofa	55%	100%
P00890	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	54%	98%
P08679	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	53%	100%
P09948	Rickettsia prowazekii (strain Madrid E)	26%	100%
P0ABH7	Escherichia coli (strain K12)	29%	100%
P0ABH8	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	29%	100%
P0C1Z2	Macaca fascicularis	55%	100%
P14165	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	28%	100%
P18789	Coxiella burnetii (strain RSA 493 / Nine Mile phase I)	27%	100%
P20115	Arabidopsis thaliana	53%	99%
P20901	Acetobacter aceti	28%	100%
P20902	Acinetobacter calcoaceticus subsp. anitratus	28%	100%
P21553	Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)	28%	100%
P23007	Gallus gallus	54%	100%
P24118	Tetrahymena thermophila	47%	100%
P27660	Weizmannia coagulans	27%	100%
P31660	Escherichia coli (strain K12)	26%	100%
P34085	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	57%	100%
P34575	Caenorhabditis elegans	53%	100%
P39119	Bacillus subtilis (strain 168)	24%	100%
P39120	Bacillus subtilis (strain 168)	25%	100%
P42457	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	26%	100%
P43635	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	42%	97%
P45858	Bacillus subtilis (strain 168)	24%	100%
P49298	Citrus maxima	53%	100%
P49299	Cucurbita maxima	25%	91%
P51031	Bartonella bacilliformis	29%	100%
P51032	Bartonella elizabethae	28%	100%
P51033	Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1)	29%	100%
P51034	Bartonella quintana (strain Toulouse)	28%	100%
P51036	Bartonella vinsonii	29%	100%
P51037	Rhizobium tropici	29%	100%
P51038	Rhizobium tropici	29%	100%
P51039	Rickettsia australis	27%	100%
P51040	Rickettsia felis (strain ATCC VR-1525 / URRWXCal2)	26%	100%
P51041	Rickettsia canadensis	26%	100%
P51042	Rickettsia conorii (strain ATCC VR-613 / Malish 7)	26%	100%
P51043	Rickettsia typhi (strain ATCC VR-144 / Wilmington)	26%	100%
P51044	Aspergillus niger	57%	99%
P51045	Acidithiobacillus ferridurans	24%	100%
P56062	Helicobacter pylori (strain ATCC 700392 / 26695)	28%	100%
P79024	Candida tropicalis	53%	100%
P80148	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	26%	100%
P83372	Fragaria ananassa	51%	100%
P94325	Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)	27%	100%
P9WPD4	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	28%	100%
P9WPD5	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	28%	100%
Q0GNE0	Iguana iguana	53%	100%
Q0GNE1	Amblyrhynchus cristatus	53%	100%
Q0QHL3	Glossina morsitans morsitans	56%	100%
Q10306	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	53%	97%
Q16P20	Aedes aegypti	55%	100%
Q17GM7	Aedes aegypti	55%	100%
Q1RGV8	Rickettsia bellii (strain RML369-C)	26%	100%
Q28DK1	Xenopus tropicalis	54%	100%
Q29RK1	Bos taurus	55%	100%
Q2TXF1	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	24%	100%
Q43175	Solanum tuberosum	50%	100%
Q4QDX2	Leishmania major	98%	100%
Q4QDX3	Leishmania major	100%	100%
Q4S5X1	Tetraodon nigroviridis	55%	100%
Q50I20	Neosartorya fumigata	49%	100%
Q53554	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	25%	100%
Q553V1	Dictyostelium discoideum	55%	100%
Q56063	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	25%	100%
Q59136	Rickettsia conorii subsp. caspia (strain A-167)	26%	100%
Q59198	Bartonella doshiae	28%	100%
Q59258	Bartonella taylorii	28%	100%
Q59730	Rickettsia akari	27%	100%
Q59732	Rickettsia africae (strain ESF-5)	26%	100%
Q59734	Rickettsia bellii	27%	100%
Q59741	Rickettsia helvetica	28%	100%
Q59742	Rickettsia japonica	27%	100%
Q59748	Rickettsia massiliae	26%	100%
Q59759	Rickettsia parkeri	26%	100%
Q59768	Rickettsia rhipicephali	26%	100%
Q59776	Rickettsia slovaca (strain 13-B)	26%	100%
Q59778	Rickettsia sibirica (strain ATCC VR-151 / 246)	26%	100%
Q59939	Streptococcus mutans serotype c (strain ATCC 700610 / UA159)	27%	100%
Q59977	Synechocystis sp. (strain PCC 6803 / Kazusa)	27%	100%
Q61JF9	Caenorhabditis briggsae	54%	100%
Q6C793	Yarrowia lipolytica (strain CLIB 122 / E 150)	53%	100%
Q6S9V5	Kajikia audax	54%	100%
Q6S9V6	Xiphias gladius	55%	100%
Q6S9V7	Katsuwonus pelamis	55%	100%
Q6S9V8	Thunnus obesus	55%	100%
Q6S9V9	Thunnus albacares	55%	100%
Q7ZVY5	Danio rerio	55%	100%
Q7ZWZ5	Xenopus laevis	55%	100%
Q86AV6	Dictyostelium discoideum	26%	92%
Q8EJW2	Shewanella oneidensis (strain MR-1)	24%	100%
Q8MQU6	Dictyostelium discoideum	24%	96%
Q8NSH7	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	26%	100%
Q8NSL1	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	28%	100%
Q8VHF5	Rattus norvegicus	54%	100%
Q937N9	Cupriavidus necator	24%	100%
Q9CZU6	Mus musculus	55%	100%
Q9LXS6	Arabidopsis thaliana	26%	91%
Q9LXS7	Arabidopsis thaliana	23%	98%
Q9M1D3	Arabidopsis thaliana	53%	100%
Q9RWB2	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	29%	100%
Q9SJH7	Arabidopsis thaliana	26%	92%
Q9TEM3	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	48%	100%
Q9W401	Drosophila melanogaster	55%	100%
Q9ZN37	Helicobacter pylori (strain J99 / ATCC 700824)	29%	100%
V5BWC7	Trypanosoma cruzi	66%	100%