Putative rab-like GTPase activating protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

disgorgin - putative

Species:

Leishmania infantum

UniProt:

A4IE19_LEIIN

TriTrypDb:

LINF_360070600

Length:

498

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 16
NetGPI	no	yes: 0, no: 16

Cellular components
Term	Name	Level	Count
GO:0000331	contractile vacuole	6	1
GO:0005773	vacuole	5	1
GO:0031410	cytoplasmic vesicle	6	1
GO:0031982	vesicle	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0097708	intracellular vesicle	5	1
GO:0110165	cellular anatomical entity	1	2
GO:0016020	membrane	2	1

Expansion

Sequence features

A4IE19

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4IE19

Function

Biological processes
Term	Name	Level	Count
GO:0043085	positive regulation of catalytic activity	4	2
GO:0043087	regulation of GTPase activity	5	2
GO:0043547	positive regulation of GTPase activity	6	2
GO:0044093	positive regulation of molecular function	3	2
GO:0050790	regulation of catalytic activity	3	2
GO:0051336	regulation of hydrolase activity	4	2
GO:0051345	positive regulation of hydrolase activity	5	2
GO:0065007	biological regulation	1	2
GO:0065009	regulation of molecular function	2	2
GO:0090630	activation of GTPase activity	7	2

Molecular functions
Term	Name	Level	Count
GO:0005096	GTPase activator activity	4	2
GO:0008047	enzyme activator activity	3	2
GO:0030234	enzyme regulator activity	2	2
GO:0030695	GTPase regulator activity	4	2
GO:0060589	nucleoside-triphosphatase regulator activity	3	2
GO:0098772	molecular function regulator activity	1	2
GO:0140677	molecular function activator activity	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	162	166	PF00656	0.582
CLV_C14_Caspase3-7	460	464	PF00656	0.463
CLV_NRD_NRD_1	200	202	PF00675	0.380
CLV_NRD_NRD_1	227	229	PF00675	0.378
CLV_NRD_NRD_1	469	471	PF00675	0.324
CLV_PCSK_KEX2_1	200	202	PF00082	0.386
CLV_PCSK_KEX2_1	371	373	PF00082	0.257
CLV_PCSK_KEX2_1	383	385	PF00082	0.244
CLV_PCSK_KEX2_1	469	471	PF00082	0.397
CLV_PCSK_PC1ET2_1	371	373	PF00082	0.257
CLV_PCSK_PC1ET2_1	383	385	PF00082	0.244
CLV_PCSK_PC7_1	196	202	PF00082	0.365
CLV_PCSK_SKI1_1	140	144	PF00082	0.564
CLV_PCSK_SKI1_1	201	205	PF00082	0.407
CLV_PCSK_SKI1_1	302	306	PF00082	0.283
CLV_PCSK_SKI1_1	353	357	PF00082	0.370
CLV_PCSK_SKI1_1	411	415	PF00082	0.256
CLV_PCSK_SKI1_1	426	430	PF00082	0.351
CLV_Separin_Metazoa	466	470	PF03568	0.372
DEG_MDM2_SWIB_1	395	403	PF02201	0.369
DEG_MDM2_SWIB_1	421	428	PF02201	0.358
DEG_SCF_FBW7_1	21	28	PF00400	0.484
DOC_CKS1_1	141	146	PF01111	0.584
DOC_CYCLIN_RxL_1	407	418	PF00134	0.252
DOC_MAPK_gen_1	380	390	PF00069	0.310
DOC_MAPK_gen_1	469	476	PF00069	0.310
DOC_PP1_RVXF_1	199	206	PF00149	0.356
DOC_PP1_RVXF_1	234	241	PF00149	0.251
DOC_PP1_RVXF_1	424	430	PF00149	0.275
DOC_PP1_RVXF_1	471	477	PF00149	0.393
DOC_PP2B_LxvP_1	444	447	PF13499	0.345
DOC_PP2B_LxvP_1	55	58	PF13499	0.665
DOC_PP4_FxxP_1	290	293	PF00568	0.225
DOC_PP4_FxxP_1	71	74	PF00568	0.483
DOC_USP7_MATH_1	32	36	PF00917	0.585
DOC_USP7_UBL2_3	214	218	PF12436	0.382
DOC_USP7_UBL2_3	266	270	PF12436	0.369
DOC_WW_Pin1_4	140	145	PF00397	0.728
DOC_WW_Pin1_4	21	26	PF00397	0.691
DOC_WW_Pin1_4	48	53	PF00397	0.626
DOC_WW_Pin1_4	66	71	PF00397	0.547
LIG_14-3-3_CanoR_1	27	36	PF00244	0.485
LIG_14-3-3_CanoR_1	7	13	PF00244	0.484
LIG_BIR_III_4	145	149	PF00653	0.528
LIG_BRCT_BRCA1_1	396	400	PF00533	0.284
LIG_BRCT_BRCA1_1	67	71	PF00533	0.529
LIG_deltaCOP1_diTrp_1	202	212	PF00928	0.403
LIG_deltaCOP1_diTrp_1	423	429	PF00928	0.285
LIG_eIF4E_1	350	356	PF01652	0.347
LIG_eIF4E_1	368	374	PF01652	0.228
LIG_FHA_1	270	276	PF00498	0.361
LIG_FHA_1	284	290	PF00498	0.375
LIG_FHA_1	40	46	PF00498	0.544
LIG_FHA_1	400	406	PF00498	0.259
LIG_FHA_1	476	482	PF00498	0.281
LIG_FHA_1	82	88	PF00498	0.518
LIG_FHA_2	130	136	PF00498	0.701
LIG_GBD_Chelix_1	324	332	PF00786	0.252
LIG_LIR_Apic_2	68	74	PF02991	0.486
LIG_LIR_Gen_1	202	210	PF02991	0.410
LIG_LIR_Gen_1	246	254	PF02991	0.355
LIG_LIR_Gen_1	35	43	PF02991	0.535
LIG_LIR_Gen_1	397	408	PF02991	0.262
LIG_LIR_Gen_1	463	471	PF02991	0.520
LIG_LIR_Gen_1	486	495	PF02991	0.434
LIG_LIR_Nem_3	172	177	PF02991	0.390
LIG_LIR_Nem_3	233	238	PF02991	0.350
LIG_LIR_Nem_3	246	250	PF02991	0.334
LIG_LIR_Nem_3	335	341	PF02991	0.274
LIG_LIR_Nem_3	35	39	PF02991	0.543
LIG_LIR_Nem_3	397	403	PF02991	0.286
LIG_LIR_Nem_3	422	428	PF02991	0.284
LIG_LIR_Nem_3	463	468	PF02991	0.533
LIG_LIR_Nem_3	486	492	PF02991	0.431
LIG_OCRL_FandH_1	337	349	PF00620	0.275
LIG_PALB2_WD40_1	395	403	PF16756	0.235
LIG_PAM2_1	390	402	PF00658	0.298
LIG_Pex14_1	417	421	PF04695	0.334
LIG_Pex14_1	425	429	PF04695	0.304
LIG_Pex14_2	356	360	PF04695	0.365
LIG_Pex14_2	395	399	PF04695	0.299
LIG_Pex14_2	400	404	PF04695	0.284
LIG_Pex14_2	421	425	PF04695	0.371
LIG_PTB_Apo_2	344	351	PF02174	0.407
LIG_PTB_Phospho_1	344	350	PF10480	0.407
LIG_SH2_PTP2	259	262	PF00017	0.253
LIG_SH2_STAT3	20	23	PF00017	0.500
LIG_SH2_STAT5	189	192	PF00017	0.434
LIG_SH2_STAT5	259	262	PF00017	0.253
LIG_SH2_STAT5	317	320	PF00017	0.283
LIG_SH3_3	355	361	PF00018	0.274
LIG_SH3_3	46	52	PF00018	0.580
LIG_SH3_3	82	88	PF00018	0.714
LIG_SUMO_SIM_anti_2	431	437	PF11976	0.323
LIG_SUMO_SIM_anti_2	50	57	PF11976	0.538
LIG_TRAF2_1	132	135	PF00917	0.567
LIG_TRAF2_1	333	336	PF00917	0.281
LIG_TRAF2_1	58	61	PF00917	0.736
LIG_UBA3_1	377	383	PF00899	0.317
LIG_UBA3_1	452	456	PF00899	0.426
LIG_WRC_WIRS_1	244	249	PF05994	0.396
LIG_WW_3	24	28	PF00397	0.515
MOD_CDK_SPxK_1	21	27	PF00069	0.515
MOD_CK1_1	28	34	PF00069	0.674
MOD_CK1_1	90	96	PF00069	0.568
MOD_CK2_1	127	133	PF00069	0.694
MOD_CK2_1	154	160	PF00069	0.565
MOD_CK2_1	32	38	PF00069	0.569
MOD_GlcNHglycan	156	159	PF01048	0.722
MOD_GlcNHglycan	27	30	PF01048	0.601
MOD_GSK3_1	125	132	PF00069	0.673
MOD_GSK3_1	136	143	PF00069	0.705
MOD_GSK3_1	21	28	PF00069	0.657
MOD_GSK3_1	390	397	PF00069	0.265
MOD_GSK3_1	438	445	PF00069	0.356
MOD_N-GLC_1	154	159	PF02516	0.644
MOD_N-GLC_1	163	168	PF02516	0.627
MOD_N-GLC_1	212	217	PF02516	0.430
MOD_NEK2_1	115	120	PF00069	0.615
MOD_NEK2_1	243	248	PF00069	0.298
MOD_NEK2_1	280	285	PF00069	0.281
MOD_NEK2_1	394	399	PF00069	0.287
MOD_NEK2_1	448	453	PF00069	0.541
MOD_NEK2_2	399	404	PF00069	0.291
MOD_PIKK_1	115	121	PF00454	0.639
MOD_PIKK_1	19	25	PF00454	0.637
MOD_PIKK_1	327	333	PF00454	0.252
MOD_PIKK_1	394	400	PF00454	0.235
MOD_PKA_2	379	385	PF00069	0.299
MOD_PKA_2	6	12	PF00069	0.475
MOD_PKA_2	65	71	PF00069	0.775
MOD_Plk_1	154	160	PF00069	0.717
MOD_Plk_1	334	340	PF00069	0.223
MOD_Plk_1	438	444	PF00069	0.347
MOD_Plk_1	448	454	PF00069	0.412
MOD_Plk_2-3	129	135	PF00069	0.673
MOD_Plk_4	296	302	PF00069	0.253
MOD_Plk_4	334	340	PF00069	0.264
MOD_Plk_4	390	396	PF00069	0.295
MOD_Plk_4	399	405	PF00069	0.320
MOD_Plk_4	448	454	PF00069	0.290
MOD_ProDKin_1	140	146	PF00069	0.727
MOD_ProDKin_1	21	27	PF00069	0.693
MOD_ProDKin_1	48	54	PF00069	0.630
MOD_ProDKin_1	66	72	PF00069	0.545
MOD_SUMO_for_1	436	439	PF00179	0.300
MOD_SUMO_rev_2	180	190	PF00179	0.523
TRG_DiLeu_BaEn_1	439	444	PF01217	0.291
TRG_DiLeu_BaEn_1	490	495	PF01217	0.311
TRG_DiLeu_BaEn_4	439	445	PF01217	0.299
TRG_DiLeu_BaLyEn_6	358	363	PF01217	0.351
TRG_DiLeu_BaLyEn_6	369	374	PF01217	0.256
TRG_DiLeu_BaLyEn_6	49	54	PF01217	0.510
TRG_ENDOCYTIC_2	259	262	PF00928	0.296
TRG_ENDOCYTIC_2	36	39	PF00928	0.543
TRG_ENDOCYTIC_2	465	468	PF00928	0.288
TRG_ENDOCYTIC_2	488	491	PF00928	0.282
TRG_ER_diArg_1	199	201	PF00400	0.347
TRG_ER_diArg_1	468	470	PF00400	0.361
TRG_ER_diArg_1	94	97	PF00400	0.609
TRG_Pf-PMV_PEXEL_1	383	387	PF00026	0.369

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDX9	Leptomonas seymouri	75%	91%
A0A0S4II34	Bodo saltans	53%	100%
A0A1X0NLM2	Trypanosomatidae	39%	100%
A0A1X0NLQ9	Trypanosomatidae	65%	100%
A0A3S7XBZ8	Leishmania donovani	41%	100%
A0A3S7XC15	Leishmania donovani	99%	100%
A0A422NJE8	Trypanosoma rangeli	37%	100%
A4HQC6	Leishmania braziliensis	79%	100%
A4HQC7	Leishmania braziliensis	40%	100%
A4IE20	Leishmania infantum	42%	100%
E9AU42	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
E9AU43	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
P58802	Mus musculus	33%	100%
Q4Q0F2	Leishmania major	35%	93%
Q4Q0F3	Leishmania major	92%	100%
Q9Y7J5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	100%
V5BT66	Trypanosoma cruzi	63%	100%