Uncharacterized protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

emp24/gp25L/p24 family/GOLD - putative

Species:

Leishmania infantum

UniProt:

A4IDX1_LEIIN

TriTrypDb:

LINF_360065400

Length:

235

Annotations

LeishMANIAdb annotations

Conserved eukaryotic protein. Probable receptor for ER-Golgi trafficking of GPI-anchored proteins. distinct from the other GOLD domain cluster (related to YB79_SCHPO instead). Localization: ER (by homology) / Golgi (by homology)

Annotations by Jardim et al.

Transporters, Uncharacterized

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 8, no: 1
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10
GO:0005783	endoplasmic reticulum	5	1
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	1
GO:0005794	Golgi apparatus	5	1
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	1
GO:0030135	coated vesicle	7	1
GO:0031410	cytoplasmic vesicle	6	1
GO:0031982	vesicle	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0097708	intracellular vesicle	5	1

Expansion

Sequence features

A4IDX1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4IDX1

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006886	intracellular protein transport	4	1
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	1
GO:0006996	organelle organization	4	1
GO:0007030	Golgi organization	5	1
GO:0008104	protein localization	4	1
GO:0009987	cellular process	1	1
GO:0015031	protein transport	4	1
GO:0016043	cellular component organization	3	1
GO:0016192	vesicle-mediated transport	4	1
GO:0033036	macromolecule localization	2	1
GO:0045184	establishment of protein localization	3	1
GO:0046907	intracellular transport	3	1
GO:0048193	Golgi vesicle transport	5	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0070727	cellular macromolecule localization	3	1
GO:0071702	organic substance transport	4	1
GO:0071705	nitrogen compound transport	4	1
GO:0071840	cellular component organization or biogenesis	2	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	188	190	PF00675	0.530
CLV_PCSK_KEX2_1	188	190	PF00082	0.530
CLV_PCSK_KEX2_1	226	228	PF00082	0.267
CLV_PCSK_KEX2_1	87	89	PF00082	0.627
CLV_PCSK_PC1ET2_1	226	228	PF00082	0.267
CLV_PCSK_PC1ET2_1	87	89	PF00082	0.627
CLV_PCSK_SKI1_1	189	193	PF00082	0.530
CLV_PCSK_SKI1_1	223	227	PF00082	0.327
DOC_CYCLIN_RxL_1	166	177	PF00134	0.302
DOC_CYCLIN_RxL_1	3	12	PF00134	0.510
DOC_MAPK_gen_1	126	132	PF00069	0.302
DOC_MAPK_gen_1	167	175	PF00069	0.296
DOC_MAPK_gen_1	87	94	PF00069	0.424
DOC_MAPK_MEF2A_6	167	175	PF00069	0.247
DOC_MAPK_MEF2A_6	6	15	PF00069	0.496
DOC_MAPK_MEF2A_6	87	94	PF00069	0.395
DOC_PP4_FxxP_1	121	124	PF00568	0.330
DOC_USP7_MATH_1	25	29	PF00917	0.554
LIG_Actin_WH2_2	71	89	PF00022	0.382
LIG_CtBP_PxDLS_1	158	162	PF00389	0.224
LIG_eIF4E_1	117	123	PF01652	0.330
LIG_FHA_1	10	16	PF00498	0.559
LIG_FHA_1	73	79	PF00498	0.355
LIG_FHA_2	175	181	PF00498	0.267
LIG_FHA_2	98	104	PF00498	0.404
LIG_LIR_Nem_3	46	50	PF02991	0.392
LIG_LIR_Nem_3	99	104	PF02991	0.349
LIG_PCNA_APIM_2	225	231	PF02747	0.502
LIG_Pex14_1	220	224	PF04695	0.447
LIG_Pex14_2	132	136	PF04695	0.247
LIG_REV1ctd_RIR_1	189	198	PF16727	0.267
LIG_SH2_CRK	34	38	PF00017	0.385
LIG_SH2_STAP1	164	168	PF00017	0.290
LIG_SH2_STAP1	34	38	PF00017	0.362
LIG_SH2_STAT5	190	193	PF00017	0.309
LIG_SH2_STAT5	224	227	PF00017	0.447
LIG_SH2_STAT5	228	231	PF00017	0.530
LIG_SH3_1	87	93	PF00018	0.364
LIG_SH3_3	140	146	PF00018	0.330
LIG_SH3_3	152	158	PF00018	0.234
LIG_SH3_3	87	93	PF00018	0.403
LIG_SUMO_SIM_anti_2	5	12	PF11976	0.335
LIG_SUMO_SIM_par_1	5	12	PF11976	0.460
LIG_UBA3_1	122	127	PF00899	0.330
LIG_Vh1_VBS_1	1	19	PF01044	0.407
LIG_WRC_WIRS_1	98	103	PF05994	0.344
MOD_CK1_1	174	180	PF00069	0.330
MOD_CK2_1	175	181	PF00069	0.265
MOD_GlcNHglycan	136	139	PF01048	0.532
MOD_GlcNHglycan	197	200	PF01048	0.552
MOD_GSK3_1	171	178	PF00069	0.330
MOD_N-GLC_1	171	176	PF02516	0.530
MOD_NEK2_1	132	137	PF00069	0.330
MOD_NEK2_1	184	189	PF00069	0.354
MOD_NEK2_1	192	197	PF00069	0.314
MOD_NEK2_1	2	7	PF00069	0.627
MOD_NEK2_2	25	30	PF00069	0.558
MOD_PIKK_1	102	108	PF00454	0.330
MOD_Plk_1	102	108	PF00069	0.330
MOD_Plk_1	132	138	PF00069	0.330
MOD_Plk_1	171	177	PF00069	0.330
MOD_Plk_1	192	198	PF00069	0.333
MOD_Plk_4	215	221	PF00069	0.453
MOD_SUMO_for_1	146	149	PF00179	0.302
TRG_DiLeu_BaEn_2	148	154	PF01217	0.330
TRG_ENDOCYTIC_2	34	37	PF00928	0.336
TRG_ER_diArg_1	167	170	PF00400	0.338
TRG_ER_diArg_1	188	190	PF00400	0.330
TRG_ER_diLys_1	231	235	PF00400	0.548

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IAH0	Leptomonas seymouri	72%	99%
A0A1X0P077	Trypanosomatidae	44%	100%
A0A3Q8IJ75	Leishmania donovani	100%	100%
A0A422NKF6	Trypanosoma rangeli	45%	100%
A4HQ73	Leishmania braziliensis	83%	100%
E9ATZ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
Q4Q0K4	Leishmania major	97%	100%
V5BTC3	Trypanosoma cruzi	43%	100%