Putative C-4 sterol methyl oxidase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

C-4 sterol methyl oxidase - putative

Species:

Leishmania infantum

UniProt:

A4ICG0_LEIIN

TriTrypDb:

LINF_360032800

Length:

402

Annotations

Annotations by Jardim et al.

Sterol metabolism/biosynthesis, C-4 sterol methyl oxidase

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005789	endoplasmic reticulum membrane	4	1
GO:0016020	membrane	2	6
GO:0031090	organelle membrane	3	1
GO:0110165	cellular anatomical entity	1	6

Expansion

Sequence features

A4ICG0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4ICG0

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	8
GO:0008152	metabolic process	1	8
GO:0008610	lipid biosynthetic process	4	8
GO:0009058	biosynthetic process	2	8
GO:0044238	primary metabolic process	2	8
GO:0071704	organic substance metabolic process	2	8
GO:1901576	organic substance biosynthetic process	3	8
GO:0006694	steroid biosynthetic process	5	1
GO:0008202	steroid metabolic process	4	1
GO:0016125	sterol metabolic process	4	1
GO:0016126	sterol biosynthetic process	5	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901615	organic hydroxy compound metabolic process	3	1
GO:1901617	organic hydroxy compound biosynthetic process	4	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	8
GO:0005488	binding	1	8
GO:0005506	iron ion binding	6	8
GO:0016491	oxidoreductase activity	2	8
GO:0043167	ion binding	2	8
GO:0043169	cation binding	3	8
GO:0046872	metal ion binding	4	8
GO:0046914	transition metal ion binding	5	8
GO:0000254	C-4 methylsterol oxidase activity	5	1
GO:0004497	monooxygenase activity	3	1
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	1
GO:0016716	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, another compound as one donor, and incorporation of one atom of oxygen	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	115	117	PF00675	0.405
CLV_NRD_NRD_1	123	125	PF00675	0.298
CLV_PCSK_KEX2_1	115	117	PF00082	0.362
CLV_PCSK_SKI1_1	190	194	PF00082	0.537
CLV_PCSK_SKI1_1	52	56	PF00082	0.511
CLV_PCSK_SKI1_1	6	10	PF00082	0.409
DEG_MDM2_SWIB_1	316	324	PF02201	0.308
DEG_Nend_UBRbox_2	1	3	PF02207	0.618
DEG_SPOP_SBC_1	372	376	PF00917	0.373
DOC_CKS1_1	251	256	PF01111	0.308
DOC_CYCLIN_yCln2_LP_2	148	154	PF00134	0.354
DOC_CYCLIN_yCln2_LP_2	63	69	PF00134	0.326
DOC_MAPK_gen_1	190	199	PF00069	0.322
DOC_MAPK_MEF2A_6	190	199	PF00069	0.290
DOC_PP2B_LxvP_1	109	112	PF13499	0.547
DOC_PP2B_LxvP_1	148	151	PF13499	0.363
DOC_PP2B_LxvP_1	166	169	PF13499	0.328
DOC_USP7_MATH_1	281	285	PF00917	0.302
DOC_USP7_UBL2_3	121	125	PF12436	0.558
DOC_USP7_UBL2_3	5	9	PF12436	0.616
DOC_WW_Pin1_4	176	181	PF00397	0.383
DOC_WW_Pin1_4	224	229	PF00397	0.275
DOC_WW_Pin1_4	250	255	PF00397	0.302
LIG_14-3-3_CanoR_1	17	25	PF00244	0.508
LIG_14-3-3_CanoR_1	373	378	PF00244	0.435
LIG_14-3-3_CanoR_1	62	66	PF00244	0.375
LIG_14-3-3_CterR_2	399	402	PF00244	0.427
LIG_Actin_WH2_2	180	195	PF00022	0.358
LIG_APCC_ABBA_1	88	93	PF00400	0.365
LIG_BRCT_BRCA1_1	347	351	PF00533	0.302
LIG_CtBP_PxDLS_1	196	200	PF00389	0.336
LIG_EH1_1	217	225	PF00400	0.279
LIG_FHA_1	171	177	PF00498	0.293
LIG_FHA_1	240	246	PF00498	0.265
LIG_FHA_1	305	311	PF00498	0.308
LIG_FHA_1	338	344	PF00498	0.259
LIG_FHA_1	36	42	PF00498	0.411
LIG_FHA_1	372	378	PF00498	0.527
LIG_FHA_2	177	183	PF00498	0.328
LIG_FHA_2	251	257	PF00498	0.308
LIG_FHA_2	275	281	PF00498	0.308
LIG_FHA_2	374	380	PF00498	0.460
LIG_LIR_Apic_2	250	254	PF02991	0.302
LIG_LIR_Gen_1	266	276	PF02991	0.381
LIG_LIR_Gen_1	298	308	PF02991	0.302
LIG_LIR_Gen_1	70	81	PF02991	0.331
LIG_LIR_Nem_3	142	147	PF02991	0.292
LIG_LIR_Nem_3	214	220	PF02991	0.346
LIG_LIR_Nem_3	229	234	PF02991	0.216
LIG_LIR_Nem_3	265	271	PF02991	0.295
LIG_LIR_Nem_3	298	304	PF02991	0.302
LIG_LIR_Nem_3	360	365	PF02991	0.397
LIG_LIR_Nem_3	70	76	PF02991	0.334
LIG_OCRL_FandH_1	233	245	PF00620	0.302
LIG_Pex14_2	269	273	PF04695	0.302
LIG_Pex14_2	316	320	PF04695	0.302
LIG_Pex14_2	69	73	PF04695	0.354
LIG_Pex14_2	85	89	PF04695	0.354
LIG_PTB_Apo_2	83	90	PF02174	0.354
LIG_SH2_CRK	120	124	PF00017	0.552
LIG_SH2_CRK	155	159	PF00017	0.363
LIG_SH2_CRK	251	255	PF00017	0.259
LIG_SH2_CRK	50	54	PF00017	0.388
LIG_SH2_STAP1	155	159	PF00017	0.363
LIG_SH2_STAT5	147	150	PF00017	0.346
LIG_SH2_STAT5	157	160	PF00017	0.242
LIG_SH2_STAT5	218	221	PF00017	0.259
LIG_SH2_STAT5	241	244	PF00017	0.259
LIG_SH2_STAT5	268	271	PF00017	0.307
LIG_SH2_STAT5	276	279	PF00017	0.311
LIG_SH2_STAT5	30	33	PF00017	0.354
LIG_SH2_STAT5	314	317	PF00017	0.271
LIG_SH2_STAT5	362	365	PF00017	0.326
LIG_SH2_STAT5	59	62	PF00017	0.403
LIG_SH3_1	120	126	PF00018	0.529
LIG_SH3_3	120	126	PF00018	0.551
LIG_SH3_3	42	48	PF00018	0.306
LIG_SH3_3	5	11	PF00018	0.580
LIG_SUMO_SIM_par_1	195	201	PF11976	0.343
LIG_TRAF2_1	254	257	PF00917	0.256
LIG_TRAF2_1	277	280	PF00917	0.308
LIG_TYR_ITIM	153	158	PF00017	0.427
LIG_WRC_WIRS_1	41	46	PF05994	0.238
MOD_CK1_1	198	204	PF00069	0.293
MOD_CK1_1	226	232	PF00069	0.285
MOD_CK1_1	282	288	PF00069	0.259
MOD_CK2_1	250	256	PF00069	0.302
MOD_CK2_1	274	280	PF00069	0.385
MOD_CK2_1	373	379	PF00069	0.431
MOD_Cter_Amidation	113	116	PF01082	0.353
MOD_GlcNHglycan	130	133	PF01048	0.453
MOD_GlcNHglycan	187	190	PF01048	0.569
MOD_GlcNHglycan	20	23	PF01048	0.276
MOD_GlcNHglycan	288	291	PF01048	0.537
MOD_GlcNHglycan	311	314	PF01048	0.459
MOD_GlcNHglycan	347	350	PF01048	0.502
MOD_GSK3_1	135	142	PF00069	0.427
MOD_GSK3_1	219	226	PF00069	0.318
MOD_GSK3_1	239	246	PF00069	0.335
MOD_GSK3_1	281	288	PF00069	0.324
MOD_LATS_1	371	377	PF00433	0.385
MOD_N-GLC_2	162	164	PF02516	0.551
MOD_N-GLC_2	342	344	PF02516	0.508
MOD_NEK2_1	1	6	PF00069	0.633
MOD_NEK2_1	134	139	PF00069	0.354
MOD_NEK2_1	158	163	PF00069	0.489
MOD_NEK2_1	286	291	PF00069	0.354
MOD_NEK2_1	295	300	PF00069	0.265
MOD_NEK2_1	316	321	PF00069	0.265
MOD_NEK2_1	337	342	PF00069	0.302
MOD_NEK2_1	55	60	PF00069	0.359
MOD_NEK2_1	91	96	PF00069	0.591
MOD_PKA_2	372	378	PF00069	0.466
MOD_PKA_2	61	67	PF00069	0.311
MOD_Plk_4	135	141	PF00069	0.357
MOD_Plk_4	170	176	PF00069	0.293
MOD_Plk_4	219	225	PF00069	0.303
MOD_Plk_4	226	232	PF00069	0.296
MOD_Plk_4	24	30	PF00069	0.296
MOD_Plk_4	282	288	PF00069	0.282
MOD_Plk_4	316	322	PF00069	0.302
MOD_Plk_4	40	46	PF00069	0.269
MOD_Plk_4	55	61	PF00069	0.451
MOD_ProDKin_1	176	182	PF00069	0.382
MOD_ProDKin_1	224	230	PF00069	0.275
MOD_ProDKin_1	250	256	PF00069	0.302
MOD_SUMO_rev_2	379	385	PF00179	0.446
TRG_DiLeu_BaLyEn_6	49	54	PF01217	0.357
TRG_ENDOCYTIC_2	147	150	PF00928	0.354
TRG_ENDOCYTIC_2	155	158	PF00928	0.234
TRG_ENDOCYTIC_2	231	234	PF00928	0.259
TRG_ENDOCYTIC_2	268	271	PF00928	0.293
TRG_ENDOCYTIC_2	50	53	PF00928	0.309
TRG_ER_diArg_1	115	117	PF00400	0.566
TRG_ER_diArg_1	331	334	PF00400	0.302
TRG_Pf-PMV_PEXEL_1	190	194	PF00026	0.563
TRG_Pf-PMV_PEXEL_1	52	56	PF00026	0.511

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEE3	Leptomonas seymouri	59%	100%
A0A0S4JXU3	Bodo saltans	37%	100%
A0A3Q8IKR9	Leishmania donovani	100%	100%
A4HPC0	Leishmania braziliensis	79%	98%
E9AT31	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
Q4Q1G7	Leishmania major	94%	100%