RNA polymerase-associated protein LEO1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA polymerase-associated protein LEO1 - putative

Species:

Leishmania infantum

UniProt:

A4IBH3_LEIIN

TriTrypDb:

LINF_350033800

Length:

554

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0008023	transcription elongation factor complex	3	12
GO:0016593	Cdc73/Paf1 complex	4	12
GO:0032991	protein-containing complex	1	12
GO:0140513	nuclear protein-containing complex	2	12
GO:0005634	nucleus	5	1
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4IBH3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4IBH3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006354	DNA-templated transcription elongation	6	12
GO:0006368	transcription elongation by RNA polymerase II	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009059	macromolecule biosynthetic process	4	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016570	histone modification	5	12
GO:0018130	heterocycle biosynthetic process	4	12
GO:0019438	aromatic compound biosynthetic process	4	12
GO:0019538	protein metabolic process	3	12
GO:0032774	RNA biosynthetic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034654	nucleobase-containing compound biosynthetic process	4	12
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044271	cellular nitrogen compound biosynthetic process	4	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901362	organic cyclic compound biosynthetic process	4	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901576	organic substance biosynthetic process	3	12
GO:0006355	regulation of DNA-templated transcription	6	1
GO:0006357	regulation of transcription by RNA polymerase II	7	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0009891	positive regulation of biosynthetic process	5	1
GO:0009893	positive regulation of metabolic process	4	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0010557	positive regulation of macromolecule biosynthetic process	6	1
GO:0010604	positive regulation of macromolecule metabolic process	5	1
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031325	positive regulation of cellular metabolic process	5	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0031328	positive regulation of cellular biosynthetic process	6	1
GO:0032784	regulation of DNA-templated transcription elongation	7	1
GO:0032786	positive regulation of DNA-templated transcription, elongation	8	1
GO:0032968	positive regulation of transcription elongation by RNA polymerase II	9	1
GO:0034243	regulation of transcription elongation by RNA polymerase II	8	1
GO:0045893	positive regulation of DNA-templated transcription	7	1
GO:0045935	positive regulation of nucleobase-containing compound metabolic process	6	1
GO:0045944	positive regulation of transcription by RNA polymerase II	8	1
GO:0048518	positive regulation of biological process	3	1
GO:0048522	positive regulation of cellular process	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051173	positive regulation of nitrogen compound metabolic process	5	1
GO:0051252	regulation of RNA metabolic process	5	1
GO:0051254	positive regulation of RNA metabolic process	6	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1902680	positive regulation of RNA biosynthetic process	7	1
GO:1903506	regulation of nucleic acid-templated transcription	7	1
GO:1903508	positive regulation of nucleic acid-templated transcription	8	1
GO:2001141	regulation of RNA biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0000993	RNA polymerase II complex binding	5	1
GO:0001098	basal transcription machinery binding	3	1
GO:0001099	basal RNA polymerase II transcription machinery binding	4	1
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0043175	RNA polymerase core enzyme binding	5	1
GO:0045309	protein phosphorylated amino acid binding	4	1
GO:0050815	phosphoserine residue binding	5	1
GO:0051219	phosphoprotein binding	3	1
GO:0070063	RNA polymerase binding	4	1
GO:0099122	RNA polymerase II C-terminal domain binding	6	1
GO:1990269	RNA polymerase II C-terminal domain phosphoserine binding	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	430	434	PF00656	0.730
CLV_C14_Caspase3-7	439	443	PF00656	0.666
CLV_NRD_NRD_1	290	292	PF00675	0.395
CLV_NRD_NRD_1	317	319	PF00675	0.419
CLV_NRD_NRD_1	341	343	PF00675	0.325
CLV_NRD_NRD_1	382	384	PF00675	0.386
CLV_PCSK_KEX2_1	149	151	PF00082	0.681
CLV_PCSK_KEX2_1	290	292	PF00082	0.370
CLV_PCSK_KEX2_1	340	342	PF00082	0.336
CLV_PCSK_KEX2_1	382	384	PF00082	0.388
CLV_PCSK_KEX2_1	398	400	PF00082	0.558
CLV_PCSK_PC1ET2_1	149	151	PF00082	0.681
CLV_PCSK_PC1ET2_1	340	342	PF00082	0.438
CLV_PCSK_PC1ET2_1	398	400	PF00082	0.692
CLV_PCSK_PC7_1	145	151	PF00082	0.553
CLV_PCSK_PC7_1	337	343	PF00082	0.365
CLV_PCSK_SKI1_1	101	105	PF00082	0.521
CLV_PCSK_SKI1_1	107	111	PF00082	0.496
CLV_PCSK_SKI1_1	152	156	PF00082	0.510
CLV_PCSK_SKI1_1	191	195	PF00082	0.441
CLV_PCSK_SKI1_1	270	274	PF00082	0.365
CLV_PCSK_SKI1_1	337	341	PF00082	0.369
CLV_PCSK_SKI1_1	347	351	PF00082	0.287
CLV_PCSK_SKI1_1	383	387	PF00082	0.331
CLV_PCSK_SKI1_1	467	471	PF00082	0.549
CLV_PCSK_SKI1_1	486	490	PF00082	0.478
CLV_PCSK_SKI1_1	70	74	PF00082	0.482
DEG_APCC_DBOX_1	485	493	PF00400	0.451
DEG_APCC_DBOX_1	69	77	PF00400	0.475
DOC_CKS1_1	194	199	PF01111	0.421
DOC_CYCLIN_yCln2_LP_2	160	166	PF00134	0.545
DOC_MAPK_gen_1	101	111	PF00069	0.574
DOC_MAPK_gen_1	340	351	PF00069	0.302
DOC_MAPK_gen_1	398	408	PF00069	0.588
DOC_PP1_RVXF_1	213	220	PF00149	0.280
DOC_PP2B_LxvP_1	160	163	PF13499	0.516
DOC_USP7_MATH_1	154	158	PF00917	0.536
DOC_USP7_MATH_1	305	309	PF00917	0.243
DOC_USP7_MATH_1	484	488	PF00917	0.451
DOC_USP7_MATH_1	498	502	PF00917	0.413
DOC_USP7_UBL2_3	340	344	PF12436	0.365
DOC_WW_Pin1_4	152	157	PF00397	0.500
DOC_WW_Pin1_4	193	198	PF00397	0.492
DOC_WW_Pin1_4	511	516	PF00397	0.539
DOC_WW_Pin1_4	8	13	PF00397	0.682
LIG_14-3-3_CanoR_1	169	173	PF00244	0.398
LIG_14-3-3_CanoR_1	202	210	PF00244	0.360
LIG_14-3-3_CanoR_1	218	226	PF00244	0.286
LIG_14-3-3_CanoR_1	347	352	PF00244	0.298
LIG_14-3-3_CanoR_1	93	99	PF00244	0.481
LIG_AP2alpha_2	421	423	PF02296	0.731
LIG_APCC_ABBA_1	109	114	PF00400	0.553
LIG_APCC_ABBA_1	86	91	PF00400	0.628
LIG_APCC_ABBAyCdc20_2	169	175	PF00400	0.393
LIG_BIR_II_1	1	5	PF00653	0.574
LIG_BRCT_BRCA1_1	188	192	PF00533	0.606
LIG_BRCT_BRCA1_1	419	423	PF00533	0.534
LIG_FHA_1	196	202	PF00498	0.276
LIG_FHA_1	223	229	PF00498	0.291
LIG_FHA_1	240	246	PF00498	0.405
LIG_FHA_1	247	253	PF00498	0.374
LIG_FHA_1	271	277	PF00498	0.421
LIG_FHA_1	537	543	PF00498	0.475
LIG_FHA_1	67	73	PF00498	0.544
LIG_FHA_1	8	14	PF00498	0.706
LIG_FHA_2	134	140	PF00498	0.651
LIG_FHA_2	17	23	PF00498	0.647
LIG_FHA_2	264	270	PF00498	0.339
LIG_FHA_2	348	354	PF00498	0.295
LIG_FHA_2	512	518	PF00498	0.543
LIG_GBD_Chelix_1	481	489	PF00786	0.509
LIG_LIR_Apic_2	313	317	PF02991	0.385
LIG_LIR_Gen_1	497	506	PF02991	0.526
LIG_LIR_Gen_1	507	515	PF02991	0.578
LIG_LIR_Nem_3	189	195	PF02991	0.553
LIG_LIR_Nem_3	476	481	PF02991	0.501
LIG_LIR_Nem_3	497	502	PF02991	0.525
LIG_LIR_Nem_3	505	511	PF02991	0.547
LIG_NRBOX	484	490	PF00104	0.460
LIG_Pex14_2	47	51	PF04695	0.466
LIG_SH2_CRK	478	482	PF00017	0.399
LIG_SH2_SRC	112	115	PF00017	0.543
LIG_SH2_SRC	186	189	PF00017	0.509
LIG_SH2_SRC	304	307	PF00017	0.249
LIG_SH2_STAT5	118	121	PF00017	0.534
LIG_SH2_STAT5	186	189	PF00017	0.514
LIG_SH2_STAT5	195	198	PF00017	0.352
LIG_SH3_3	161	167	PF00018	0.603
LIG_SH3_3	175	181	PF00018	0.333
LIG_SUMO_SIM_anti_2	231	238	PF11976	0.473
LIG_TRAF2_1	237	240	PF00917	0.405
LIG_WRC_WIRS_1	441	446	PF05994	0.526
MOD_CK1_1	211	217	PF00069	0.401
MOD_CK1_1	220	226	PF00069	0.241
MOD_CK1_1	238	244	PF00069	0.298
MOD_CK1_1	471	477	PF00069	0.617
MOD_CK2_1	133	139	PF00069	0.638
MOD_CK2_1	263	269	PF00069	0.427
MOD_CK2_1	319	325	PF00069	0.340
MOD_CK2_1	347	353	PF00069	0.280
MOD_DYRK1A_RPxSP_1	152	156	PF00069	0.484
MOD_GlcNHglycan	120	123	PF01048	0.618
MOD_GlcNHglycan	368	371	PF01048	0.346
MOD_GlcNHglycan	413	416	PF01048	0.723
MOD_GlcNHglycan	550	553	PF01048	0.385
MOD_GlcNHglycan	94	97	PF01048	0.615
MOD_GSK3_1	133	140	PF00069	0.543
MOD_GSK3_1	231	238	PF00069	0.316
MOD_GSK3_1	436	443	PF00069	0.708
MOD_GSK3_1	498	505	PF00069	0.430
MOD_GSK3_1	507	514	PF00069	0.438
MOD_N-GLC_1	511	516	PF02516	0.607
MOD_NEK2_1	137	142	PF00069	0.514
MOD_NEK2_1	201	206	PF00069	0.352
MOD_NEK2_1	39	44	PF00069	0.652
MOD_NEK2_1	66	71	PF00069	0.451
MOD_NEK2_2	222	227	PF00069	0.280
MOD_OFUCOSY	179	184	PF10250	0.581
MOD_PIKK_1	235	241	PF00454	0.376
MOD_PIKK_1	25	31	PF00454	0.733
MOD_PIKK_1	536	542	PF00454	0.492
MOD_PK_1	319	325	PF00069	0.313
MOD_PKA_2	168	174	PF00069	0.412
MOD_PKA_2	201	207	PF00069	0.365
MOD_PKA_2	217	223	PF00069	0.316
MOD_PKA_2	92	98	PF00069	0.496
MOD_Plk_1	138	144	PF00069	0.575
MOD_Plk_1	239	245	PF00069	0.410
MOD_Plk_1	305	311	PF00069	0.414
MOD_Plk_1	39	45	PF00069	0.601
MOD_Plk_2-3	278	284	PF00069	0.365
MOD_Plk_2-3	429	435	PF00069	0.662
MOD_Plk_2-3	436	442	PF00069	0.577
MOD_Plk_4	154	160	PF00069	0.516
MOD_Plk_4	231	237	PF00069	0.410
MOD_Plk_4	504	510	PF00069	0.491
MOD_ProDKin_1	152	158	PF00069	0.489
MOD_ProDKin_1	193	199	PF00069	0.403
MOD_ProDKin_1	511	517	PF00069	0.540
MOD_ProDKin_1	8	14	PF00069	0.678
MOD_SUMO_for_1	469	472	PF00179	0.617
MOD_SUMO_for_1	542	545	PF00179	0.522
MOD_SUMO_rev_2	97	105	PF00179	0.580
TRG_DiLeu_BaEn_2	187	193	PF01217	0.584
TRG_ENDOCYTIC_2	478	481	PF00928	0.405
TRG_ER_diArg_1	290	293	PF00400	0.395
TRG_ER_diArg_1	341	343	PF00400	0.436
TRG_ER_diArg_1	381	383	PF00400	0.380
TRG_ER_diArg_1	399	402	PF00400	0.498
TRG_NLS_Bipartite_1	382	402	PF00514	0.628
TRG_NLS_MonoExtC_3	339	344	PF00514	0.437
TRG_NLS_MonoExtC_3	397	403	PF00514	0.690
TRG_NLS_MonoExtN_4	316	322	PF00514	0.421
TRG_NLS_MonoExtN_4	337	344	PF00514	0.365
TRG_NLS_MonoExtN_4	395	402	PF00514	0.685
TRG_Pf-PMV_PEXEL_1	321	325	PF00026	0.421
TRG_Pf-PMV_PEXEL_1	341	345	PF00026	0.421
TRG_Pf-PMV_PEXEL_1	486	491	PF00026	0.542
TRG_Pf-PMV_PEXEL_1	525	529	PF00026	0.546

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMF7	Leptomonas seymouri	65%	97%
A0A0S4JFB0	Bodo saltans	27%	85%
A0A1X0P5N0	Trypanosomatidae	38%	92%
A0A3Q8IMF9	Leishmania donovani	99%	100%
A0A3R7P349	Trypanosoma rangeli	36%	96%
A4HMV2	Leishmania braziliensis	84%	100%
C9ZZ69	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	96%
E9AFA9	Leishmania major	96%	100%
E9B6G4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
V5BD17	Trypanosoma cruzi	38%	92%