rRNA adenine N(6)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Protein of unknown function (DUF2946), putative

Species:

Leishmania infantum

UniProt:

A4IAX5_LEIIN

TriTrypDb:

Length:

463

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4IAX5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4IAX5

Function

Biological processes
Term	Name	Level	Count
GO:0000154	rRNA modification	6	1
GO:0001510	RNA methylation	4	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006364	rRNA processing	8	1
GO:0006396	RNA processing	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0016072	rRNA metabolic process	7	1
GO:0031167	rRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000179	rRNA (adenine-N6,N6-)-dimethyltransferase activity	6	11
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0005488	binding	1	11
GO:0008168	methyltransferase activity	4	11
GO:0008170	N-methyltransferase activity	5	11
GO:0008173	RNA methyltransferase activity	4	11
GO:0008649	rRNA methyltransferase activity	5	11
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	11
GO:0016433	rRNA (adenine) methyltransferase activity	6	11
GO:0016740	transferase activity	2	11
GO:0016741	transferase activity, transferring one-carbon groups	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140102	catalytic activity, acting on a rRNA	4	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	106	112	PF00089	0.194
CLV_NRD_NRD_1	154	156	PF00675	0.431
CLV_NRD_NRD_1	212	214	PF00675	0.490
CLV_NRD_NRD_1	453	455	PF00675	0.537
CLV_PCSK_FUR_1	210	214	PF00082	0.544
CLV_PCSK_KEX2_1	154	156	PF00082	0.431
CLV_PCSK_KEX2_1	212	214	PF00082	0.488
CLV_PCSK_SKI1_1	142	146	PF00082	0.509
CLV_PCSK_SKI1_1	285	289	PF00082	0.369
CLV_PCSK_SKI1_1	382	386	PF00082	0.255
CLV_PCSK_SKI1_1	52	56	PF00082	0.240
CLV_PCSK_SKI1_1	91	95	PF00082	0.249
DEG_ODPH_VHL_1	308	321	PF01847	0.453
DOC_ANK_TNKS_1	225	232	PF00023	0.551
DOC_ANK_TNKS_1	27	34	PF00023	0.392
DOC_ANK_TNKS_1	342	349	PF00023	0.535
DOC_CKS1_1	72	77	PF01111	0.497
DOC_CYCLIN_RxL_1	88	95	PF00134	0.440
DOC_MAPK_MEF2A_6	52	59	PF00069	0.440
DOC_MAPK_NFAT4_5	52	60	PF00069	0.440
DOC_PP1_RVXF_1	178	184	PF00149	0.600
DOC_USP7_MATH_1	165	169	PF00917	0.676
DOC_USP7_MATH_1	170	174	PF00917	0.680
DOC_USP7_MATH_1	199	203	PF00917	0.592
DOC_USP7_MATH_1	310	314	PF00917	0.452
DOC_USP7_MATH_1	4	8	PF00917	0.694
DOC_USP7_UBL2_3	35	39	PF12436	0.375
DOC_WW_Pin1_4	108	113	PF00397	0.394
DOC_WW_Pin1_4	155	160	PF00397	0.568
DOC_WW_Pin1_4	237	242	PF00397	0.684
DOC_WW_Pin1_4	455	460	PF00397	0.721
DOC_WW_Pin1_4	6	11	PF00397	0.579
DOC_WW_Pin1_4	71	76	PF00397	0.514
LIG_14-3-3_CanoR_1	118	127	PF00244	0.400
LIG_14-3-3_CanoR_1	172	181	PF00244	0.540
LIG_14-3-3_CanoR_1	318	322	PF00244	0.440
LIG_14-3-3_CanoR_1	330	336	PF00244	0.440
LIG_14-3-3_CanoR_1	382	387	PF00244	0.441
LIG_14-3-3_CanoR_1	69	75	PF00244	0.440
LIG_Actin_WH2_2	128	144	PF00022	0.475
LIG_Actin_WH2_2	368	384	PF00022	0.492
LIG_APCC_ABBA_1	110	115	PF00400	0.535
LIG_BIR_III_4	191	195	PF00653	0.593
LIG_BRCT_BRCA1_1	121	125	PF00533	0.467
LIG_BRCT_BRCA1_1	312	316	PF00533	0.440
LIG_FHA_1	127	133	PF00498	0.556
LIG_FHA_1	269	275	PF00498	0.450
LIG_FHA_1	371	377	PF00498	0.458
LIG_FHA_1	58	64	PF00498	0.470
LIG_FHA_1	88	94	PF00498	0.445
LIG_FHA_2	180	186	PF00498	0.598
LIG_FHA_2	297	303	PF00498	0.425
LIG_FHA_2	332	338	PF00498	0.440
LIG_FHA_2	342	348	PF00498	0.440
LIG_FHA_2	411	417	PF00498	0.440
LIG_FHA_2	71	77	PF00498	0.535
LIG_LIR_Gen_1	122	133	PF02991	0.407
LIG_LIR_Gen_1	313	322	PF02991	0.440
LIG_LIR_Nem_3	122	128	PF02991	0.403
LIG_LIR_Nem_3	143	148	PF02991	0.365
LIG_LIR_Nem_3	313	319	PF02991	0.440
LIG_PTB_Apo_2	261	268	PF02174	0.440
LIG_REV1ctd_RIR_1	324	334	PF16727	0.492
LIG_SH2_CRK	38	42	PF00017	0.504
LIG_SH2_SRC	218	221	PF00017	0.649
LIG_SH2_STAP1	49	53	PF00017	0.453
LIG_SH2_STAT3	401	404	PF00017	0.440
LIG_SH2_STAT5	120	123	PF00017	0.445
LIG_SH2_STAT5	66	69	PF00017	0.535
LIG_SH3_3	13	19	PF00018	0.603
LIG_SH3_3	153	159	PF00018	0.553
LIG_SH3_3	21	27	PF00018	0.434
LIG_SUMO_SIM_anti_2	136	143	PF11976	0.457
LIG_SUMO_SIM_anti_2	317	323	PF11976	0.440
LIG_SUMO_SIM_anti_2	416	421	PF11976	0.492
LIG_SUMO_SIM_par_1	349	354	PF11976	0.535
LIG_SUMO_SIM_par_1	413	418	PF11976	0.453
LIG_TRAF2_1	159	162	PF00917	0.656
LIG_TRAF2_2	217	222	PF00917	0.547
LIG_UBA3_1	54	58	PF00899	0.440
MOD_CDK_SPxK_1	71	77	PF00069	0.497
MOD_CK1_1	119	125	PF00069	0.394
MOD_CK1_1	173	179	PF00069	0.699
MOD_CK1_1	221	227	PF00069	0.789
MOD_CK1_1	317	323	PF00069	0.440
MOD_CK1_1	5	11	PF00069	0.665
MOD_CK1_1	65	71	PF00069	0.458
MOD_CK1_1	92	98	PF00069	0.440
MOD_CK2_1	296	302	PF00069	0.470
MOD_CK2_1	341	347	PF00069	0.471
MOD_CK2_1	415	421	PF00069	0.458
MOD_DYRK1A_RPxSP_1	155	159	PF00069	0.537
MOD_GlcNHglycan	142	145	PF01048	0.543
MOD_GlcNHglycan	167	170	PF01048	0.684
MOD_GlcNHglycan	175	178	PF01048	0.688
MOD_GlcNHglycan	201	204	PF01048	0.584
MOD_GlcNHglycan	4	7	PF01048	0.659
MOD_GSK3_1	171	178	PF00069	0.688
MOD_GSK3_1	2	9	PF00069	0.586
MOD_GSK3_1	310	317	PF00069	0.440
MOD_GSK3_1	382	389	PF00069	0.525
MOD_GSK3_1	67	74	PF00069	0.440
MOD_GSK3_1	85	92	PF00069	0.456
MOD_NEK2_1	140	145	PF00069	0.585
MOD_NEK2_1	245	250	PF00069	0.527
MOD_NEK2_1	331	336	PF00069	0.477
MOD_NEK2_1	364	369	PF00069	0.568
MOD_NEK2_1	415	420	PF00069	0.522
MOD_NEK2_1	57	62	PF00069	0.470
MOD_NEK2_1	67	72	PF00069	0.350
MOD_NEK2_1	89	94	PF00069	0.440
MOD_PIKK_1	175	181	PF00454	0.752
MOD_PIKK_1	279	285	PF00454	0.535
MOD_PIKK_1	296	302	PF00454	0.365
MOD_PIKK_1	433	439	PF00454	0.414
MOD_PKA_2	171	177	PF00069	0.635
MOD_PKA_2	179	185	PF00069	0.770
MOD_PKA_2	317	323	PF00069	0.440
MOD_PKA_2	68	74	PF00069	0.440
MOD_Plk_1	336	342	PF00069	0.440
MOD_Plk_1	364	370	PF00069	0.448
MOD_Plk_1	415	421	PF00069	0.535
MOD_Plk_4	317	323	PF00069	0.446
MOD_Plk_4	331	337	PF00069	0.512
MOD_Plk_4	365	371	PF00069	0.470
MOD_Plk_4	410	416	PF00069	0.440
MOD_Plk_4	62	68	PF00069	0.535
MOD_ProDKin_1	108	114	PF00069	0.394
MOD_ProDKin_1	155	161	PF00069	0.576
MOD_ProDKin_1	237	243	PF00069	0.680
MOD_ProDKin_1	455	461	PF00069	0.690
MOD_ProDKin_1	6	12	PF00069	0.578
MOD_ProDKin_1	71	77	PF00069	0.514
MOD_SUMO_for_1	257	260	PF00179	0.440
MOD_SUMO_for_1	29	32	PF00179	0.375
TRG_DiLeu_BaLyEn_6	204	209	PF01217	0.567
TRG_ER_diArg_1	153	155	PF00400	0.427
TRG_ER_diArg_1	209	212	PF00400	0.443
TRG_NES_CRM1_1	397	409	PF08389	0.535
TRG_Pf-PMV_PEXEL_1	207	211	PF00026	0.514
TRG_Pf-PMV_PEXEL_1	77	81	PF00026	0.257

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HXZ3	Leptomonas seymouri	78%	86%
A0A1X0NJH8	Trypanosomatidae	56%	87%
A0A3R7NNC2	Trypanosoma rangeli	58%	96%
A0A3S7X8W9	Leishmania donovani	100%	100%
A4HMA9	Leishmania braziliensis	88%	100%
D0A280	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	99%
E9AEQ2	Leishmania major	94%	100%
E9B5V7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
V5BN79	Trypanosoma cruzi	61%	97%