N2227 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

N2227-like protein - putative

Species:

Leishmania infantum

UniProt:

A4I9R0_LEIIN

TriTrypDb:

LINF_340016500

Length:

456

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0010494	cytoplasmic stress granule	5	1
GO:0035770	ribonucleoprotein granule	3	1
GO:0036464	cytoplasmic ribonucleoprotein granule	4	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0099080	supramolecular complex	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4I9R0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I9R0

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	301	305	PF00656	0.437
CLV_NRD_NRD_1	108	110	PF00675	0.494
CLV_PCSK_KEX2_1	94	96	PF00082	0.452
CLV_PCSK_PC1ET2_1	94	96	PF00082	0.452
CLV_PCSK_SKI1_1	116	120	PF00082	0.437
CLV_PCSK_SKI1_1	202	206	PF00082	0.279
CLV_PCSK_SKI1_1	240	244	PF00082	0.297
CLV_PCSK_SKI1_1	421	425	PF00082	0.212
CLV_PCSK_SKI1_1	429	433	PF00082	0.212
CLV_PCSK_SKI1_1	95	99	PF00082	0.463
DOC_CKS1_1	169	174	PF01111	0.479
DOC_CKS1_1	22	27	PF01111	0.646
DOC_MAPK_DCC_7	165	175	PF00069	0.288
DOC_MAPK_gen_1	215	225	PF00069	0.439
DOC_MAPK_MEF2A_6	116	123	PF00069	0.384
DOC_MAPK_MEF2A_6	218	225	PF00069	0.414
DOC_MAPK_MEF2A_6	232	239	PF00069	0.417
DOC_PP1_RVXF_1	307	314	PF00149	0.438
DOC_PP1_RVXF_1	398	404	PF00149	0.455
DOC_PP2B_LxvP_1	361	364	PF13499	0.423
DOC_PP2B_LxvP_1	56	59	PF13499	0.603
DOC_USP7_UBL2_3	125	129	PF12436	0.446
DOC_WW_Pin1_4	168	173	PF00397	0.428
DOC_WW_Pin1_4	21	26	PF00397	0.767
LIG_14-3-3_CanoR_1	232	236	PF00244	0.416
LIG_APCC_ABBA_1	272	277	PF00400	0.412
LIG_BIR_II_1	1	5	PF00653	0.699
LIG_BRCT_BRCA1_1	137	141	PF00533	0.289
LIG_Clathr_ClatBox_1	118	122	PF01394	0.495
LIG_deltaCOP1_diTrp_1	153	163	PF00928	0.344
LIG_FHA_1	277	283	PF00498	0.423
LIG_FHA_1	333	339	PF00498	0.512
LIG_FHA_2	169	175	PF00498	0.440
LIG_GBD_Chelix_1	397	405	PF00786	0.255
LIG_GSK3_LRP6_1	168	173	PF00069	0.479
LIG_LIR_Apic_2	324	330	PF02991	0.463
LIG_LIR_Gen_1	138	147	PF02991	0.336
LIG_LIR_Gen_1	248	259	PF02991	0.412
LIG_LIR_Gen_1	287	297	PF02991	0.426
LIG_LIR_Gen_1	322	330	PF02991	0.412
LIG_LIR_Gen_1	335	344	PF02991	0.412
LIG_LIR_Gen_1	353	362	PF02991	0.348
LIG_LIR_Nem_3	106	111	PF02991	0.441
LIG_LIR_Nem_3	138	144	PF02991	0.363
LIG_LIR_Nem_3	149	155	PF02991	0.357
LIG_LIR_Nem_3	198	204	PF02991	0.426
LIG_LIR_Nem_3	248	254	PF02991	0.412
LIG_LIR_Nem_3	287	292	PF02991	0.444
LIG_LIR_Nem_3	312	316	PF02991	0.439
LIG_LIR_Nem_3	322	326	PF02991	0.385
LIG_LIR_Nem_3	335	339	PF02991	0.412
LIG_LIR_Nem_3	353	357	PF02991	0.348
LIG_MLH1_MIPbox_1	137	141	PF16413	0.280
LIG_MYND_1	21	25	PF01753	0.598
LIG_PCNA_yPIPBox_3	420	429	PF02747	0.455
LIG_Pex14_2	152	156	PF04695	0.317
LIG_Pex14_2	185	189	PF04695	0.412
LIG_PTB_Apo_2	259	266	PF02174	0.512
LIG_Rb_LxCxE_1	128	149	PF01857	0.280
LIG_SH2_CRK	201	205	PF00017	0.455
LIG_SH2_CRK	251	255	PF00017	0.412
LIG_SH2_CRK	327	331	PF00017	0.501
LIG_SH2_CRK	359	363	PF00017	0.423
LIG_SH2_GRB2like	416	419	PF00017	0.423
LIG_SH2_SRC	61	64	PF00017	0.508
LIG_SH2_STAT3	93	96	PF00017	0.374
LIG_SH2_STAT5	130	133	PF00017	0.368
LIG_SH2_STAT5	259	262	PF00017	0.421
LIG_SH2_STAT5	354	357	PF00017	0.412
LIG_SH2_STAT5	416	419	PF00017	0.415
LIG_SH3_3	164	170	PF00018	0.267
LIG_SH3_3	19	25	PF00018	0.735
LIG_SH3_3	197	203	PF00018	0.423
LIG_SH3_3	220	226	PF00018	0.479
LIG_SH3_3	289	295	PF00018	0.423
LIG_SH3_3	40	46	PF00018	0.480
LIG_SH3_3	60	66	PF00018	0.440
LIG_SH3_3	68	74	PF00018	0.505
LIG_SUMO_SIM_anti_2	353	359	PF11976	0.423
LIG_TRAF2_1	147	150	PF00917	0.434
LIG_TYR_ITIM	352	357	PF00017	0.412
LIG_UBA3_1	118	125	PF00899	0.481
LIG_WW_1	45	48	PF00397	0.464
MOD_CK1_1	276	282	PF00069	0.512
MOD_CK1_1	49	55	PF00069	0.553
MOD_CK2_1	387	393	PF00069	0.412
MOD_GlcNHglycan	1	4	PF01048	0.716
MOD_GlcNHglycan	137	140	PF01048	0.362
MOD_GlcNHglycan	188	192	PF01048	0.212
MOD_GlcNHglycan	380	383	PF01048	0.283
MOD_GlcNHglycan	51	54	PF01048	0.552
MOD_GSK3_1	10	17	PF00069	0.627
MOD_GSK3_1	334	341	PF00069	0.499
MOD_GSK3_1	383	390	PF00069	0.420
MOD_GSK3_1	407	414	PF00069	0.512
MOD_GSK3_1	79	86	PF00069	0.330
MOD_NEK2_1	103	108	PF00069	0.218
MOD_NEK2_1	151	156	PF00069	0.402
MOD_NEK2_1	344	349	PF00069	0.417
MOD_NEK2_1	411	416	PF00069	0.413
MOD_NEK2_1	83	88	PF00069	0.319
MOD_PK_1	334	340	PF00069	0.477
MOD_PKA_2	103	109	PF00069	0.453
MOD_PKA_2	231	237	PF00069	0.458
MOD_PKA_2	5	11	PF00069	0.517
MOD_Plk_1	247	253	PF00069	0.412
MOD_Plk_1	383	389	PF00069	0.435
MOD_Plk_1	412	418	PF00069	0.412
MOD_Plk_1	447	453	PF00069	0.432
MOD_Plk_2-3	383	389	PF00069	0.437
MOD_Plk_4	151	157	PF00069	0.384
MOD_Plk_4	158	164	PF00069	0.392
MOD_Plk_4	314	320	PF00069	0.412
MOD_Plk_4	407	413	PF00069	0.512
MOD_Plk_4	79	85	PF00069	0.416
MOD_ProDKin_1	168	174	PF00069	0.428
MOD_ProDKin_1	21	27	PF00069	0.764
MOD_SUMO_rev_2	448	456	PF00179	0.577
TRG_DiLeu_BaLyEn_6	370	375	PF01217	0.412
TRG_ENDOCYTIC_2	201	204	PF00928	0.412
TRG_ENDOCYTIC_2	251	254	PF00928	0.412
TRG_ENDOCYTIC_2	354	357	PF00928	0.412
TRG_ENDOCYTIC_2	359	362	PF00928	0.412
TRG_Pf-PMV_PEXEL_1	109	113	PF00026	0.501
TRG_Pf-PMV_PEXEL_1	437	441	PF00026	0.365

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2M2	Leptomonas seymouri	85%	100%
A0A0S4IZ95	Bodo saltans	53%	100%
A0A1X0PA60	Trypanosomatidae	70%	100%
A0A3Q8IH25	Leishmania donovani	100%	100%
A0A3S5IQL4	Trypanosoma rangeli	73%	100%
A4HAL5	Leishmania braziliensis	89%	100%
C9ZMY1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	65%	100%
E9B4S4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
F1N9S8	Gallus gallus	39%	100%
P53934	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	100%
Q4Q3A3	Leishmania major	97%	100%
Q54ST2	Dictyostelium discoideum	36%	98%
Q5BJZ6	Rattus norvegicus	37%	100%
Q80UY1	Mus musculus	37%	100%
Q8N4J0	Homo sapiens	36%	100%
Q9Y7J3	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	40%	100%
V5BEW2	Trypanosoma cruzi	67%	100%