Uncharacterized protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase - putative

Species:

Leishmania infantum

UniProt:

A4I9K1_LEIIN

TriTrypDb:

LINF_340008200

Length:

701

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	4
GO:0043227	membrane-bounded organelle	3	4
GO:0043229	intracellular organelle	3	4
GO:0043231	intracellular membrane-bounded organelle	4	4
GO:0110165	cellular anatomical entity	1	4

Expansion

Sequence features

A4I9K1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I9K1

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	8
GO:0006139	nucleobase-containing compound metabolic process	3	8
GO:0006396	RNA processing	6	8
GO:0006399	tRNA metabolic process	7	8
GO:0006725	cellular aromatic compound metabolic process	3	8
GO:0006807	nitrogen compound metabolic process	2	8
GO:0008033	tRNA processing	8	8
GO:0008152	metabolic process	1	8
GO:0009451	RNA modification	5	8
GO:0009987	cellular process	1	8
GO:0016070	RNA metabolic process	5	8
GO:0034470	ncRNA processing	7	8
GO:0034641	cellular nitrogen compound metabolic process	3	8
GO:0034660	ncRNA metabolic process	6	8
GO:0043170	macromolecule metabolic process	3	8
GO:0043412	macromolecule modification	4	8
GO:0044237	cellular metabolic process	2	8
GO:0044238	primary metabolic process	2	8
GO:0046483	heterocycle metabolic process	3	8
GO:0071704	organic substance metabolic process	2	8
GO:0090304	nucleic acid metabolic process	4	8
GO:1901360	organic cyclic compound metabolic process	3	8
GO:0006400	tRNA modification	6	4
GO:0016071	mRNA metabolic process	6	1
GO:0016556	mRNA modification	6	1
GO:0031119	tRNA pseudouridine synthesis	7	4
GO:1990481	mRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	8
GO:0003723	RNA binding	4	8
GO:0003824	catalytic activity	1	8
GO:0005488	binding	1	8
GO:0009982	pseudouridine synthase activity	4	8
GO:0016853	isomerase activity	2	8
GO:0016866	intramolecular transferase activity	3	8
GO:0097159	organic cyclic compound binding	2	8
GO:1901363	heterocyclic compound binding	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	374	378	PF00656	0.775
CLV_NRD_NRD_1	219	221	PF00675	0.403
CLV_NRD_NRD_1	282	284	PF00675	0.652
CLV_NRD_NRD_1	289	291	PF00675	0.672
CLV_NRD_NRD_1	298	300	PF00675	0.649
CLV_NRD_NRD_1	53	55	PF00675	0.472
CLV_NRD_NRD_1	578	580	PF00675	0.345
CLV_NRD_NRD_1	603	605	PF00675	0.430
CLV_NRD_NRD_1	608	610	PF00675	0.430
CLV_NRD_NRD_1	631	633	PF00675	0.514
CLV_NRD_NRD_1	665	667	PF00675	0.448
CLV_PCSK_FUR_1	51	55	PF00082	0.560
CLV_PCSK_KEX2_1	145	147	PF00082	0.542
CLV_PCSK_KEX2_1	187	189	PF00082	0.441
CLV_PCSK_KEX2_1	219	221	PF00082	0.447
CLV_PCSK_KEX2_1	264	266	PF00082	0.746
CLV_PCSK_KEX2_1	281	283	PF00082	0.528
CLV_PCSK_KEX2_1	298	300	PF00082	0.623
CLV_PCSK_KEX2_1	50	52	PF00082	0.557
CLV_PCSK_KEX2_1	53	55	PF00082	0.455
CLV_PCSK_KEX2_1	534	536	PF00082	0.326
CLV_PCSK_KEX2_1	608	610	PF00082	0.430
CLV_PCSK_KEX2_1	631	633	PF00082	0.514
CLV_PCSK_KEX2_1	664	666	PF00082	0.448
CLV_PCSK_KEX2_1	697	699	PF00082	0.396
CLV_PCSK_PC1ET2_1	145	147	PF00082	0.637
CLV_PCSK_PC1ET2_1	187	189	PF00082	0.495
CLV_PCSK_PC1ET2_1	264	266	PF00082	0.746
CLV_PCSK_PC1ET2_1	298	300	PF00082	0.790
CLV_PCSK_PC1ET2_1	50	52	PF00082	0.606
CLV_PCSK_PC1ET2_1	534	536	PF00082	0.326
CLV_PCSK_PC1ET2_1	697	699	PF00082	0.396
CLV_PCSK_PC7_1	604	610	PF00082	0.430
CLV_PCSK_PC7_1	693	699	PF00082	0.366
CLV_PCSK_SKI1_1	187	191	PF00082	0.426
CLV_PCSK_SKI1_1	269	273	PF00082	0.817
CLV_PCSK_SKI1_1	300	304	PF00082	0.756
CLV_PCSK_SKI1_1	439	443	PF00082	0.425
CLV_PCSK_SKI1_1	599	603	PF00082	0.430
CLV_PCSK_SKI1_1	689	693	PF00082	0.547
CLV_PCSK_SKI1_1	89	93	PF00082	0.486
CLV_Separin_Metazoa	562	566	PF03568	0.376
DEG_ODPH_VHL_1	245	257	PF01847	0.508
DEG_SPOP_SBC_1	319	323	PF00917	0.613
DOC_CKS1_1	22	27	PF01111	0.581
DOC_CYCLIN_RxL_1	436	446	PF00134	0.570
DOC_CYCLIN_RxL_1	86	95	PF00134	0.483
DOC_MAPK_gen_1	50	60	PF00069	0.463
DOC_MAPK_gen_1	664	672	PF00069	0.475
DOC_MAPK_gen_1	686	694	PF00069	0.418
DOC_MAPK_MEF2A_6	127	136	PF00069	0.403
DOC_MAPK_MEF2A_6	53	62	PF00069	0.418
DOC_MAPK_MEF2A_6	664	672	PF00069	0.422
DOC_PP1_RVXF_1	251	258	PF00149	0.611
DOC_PP1_RVXF_1	691	697	PF00149	0.491
DOC_PP4_FxxP_1	244	247	PF00568	0.473
DOC_PP4_FxxP_1	499	502	PF00568	0.430
DOC_USP7_MATH_1	2	6	PF00917	0.693
DOC_USP7_MATH_1	289	293	PF00917	0.651
DOC_USP7_MATH_1	525	529	PF00917	0.424
DOC_USP7_UBL2_3	249	253	PF12436	0.436
DOC_WW_Pin1_4	21	26	PF00397	0.653
DOC_WW_Pin1_4	320	325	PF00397	0.770
DOC_WW_Pin1_4	348	353	PF00397	0.739
DOC_WW_Pin1_4	35	40	PF00397	0.591
DOC_WW_Pin1_4	452	457	PF00397	0.341
DOC_WW_Pin1_4	478	483	PF00397	0.430
DOC_WW_Pin1_4	498	503	PF00397	0.171
LIG_14-3-3_CanoR_1	115	123	PF00244	0.455
LIG_14-3-3_CanoR_1	127	133	PF00244	0.401
LIG_14-3-3_CanoR_1	269	274	PF00244	0.732
LIG_14-3-3_CanoR_1	354	360	PF00244	0.712
LIG_14-3-3_CanoR_1	467	473	PF00244	0.365
LIG_14-3-3_CanoR_1	505	511	PF00244	0.430
LIG_Actin_WH2_2	238	255	PF00022	0.611
LIG_Actin_WH2_2	678	695	PF00022	0.540
LIG_AP2alpha_2	476	478	PF02296	0.421
LIG_APCC_ABBA_1	345	350	PF00400	0.548
LIG_BRCT_BRCA1_1	474	478	PF00533	0.376
LIG_Clathr_ClatBox_1	110	114	PF01394	0.509
LIG_eIF4E_1	597	603	PF01652	0.410
LIG_FHA_1	225	231	PF00498	0.452
LIG_FHA_1	320	326	PF00498	0.807
LIG_FHA_1	335	341	PF00498	0.553
LIG_FHA_1	354	360	PF00498	0.589
LIG_FHA_1	517	523	PF00498	0.408
LIG_FHA_1	646	652	PF00498	0.597
LIG_FHA_1	92	98	PF00498	0.448
LIG_FHA_2	11	17	PF00498	0.711
LIG_FHA_2	230	236	PF00498	0.469
LIG_FHA_2	249	255	PF00498	0.348
LIG_FHA_2	270	276	PF00498	0.770
LIG_FHA_2	507	513	PF00498	0.430
LIG_FHA_2	80	86	PF00498	0.437
LIG_GBD_Chelix_1	668	676	PF00786	0.406
LIG_LIR_Apic_2	222	226	PF02991	0.397
LIG_LIR_Gen_1	227	236	PF02991	0.413
LIG_LIR_Gen_1	405	414	PF02991	0.517
LIG_LIR_Gen_1	448	456	PF02991	0.362
LIG_LIR_Gen_1	622	629	PF02991	0.407
LIG_LIR_Gen_1	99	110	PF02991	0.597
LIG_LIR_Nem_3	207	213	PF02991	0.444
LIG_LIR_Nem_3	239	244	PF02991	0.514
LIG_LIR_Nem_3	38	43	PF02991	0.650
LIG_LIR_Nem_3	448	454	PF02991	0.362
LIG_LIR_Nem_3	464	469	PF02991	0.376
LIG_LIR_Nem_3	622	626	PF02991	0.376
LIG_LIR_Nem_3	67	72	PF02991	0.410
LIG_LIR_Nem_3	79	83	PF02991	0.454
LIG_LIR_Nem_3	99	105	PF02991	0.273
LIG_LYPXL_yS_3	80	83	PF13949	0.496
LIG_MYND_1	28	32	PF01753	0.565
LIG_PCNA_yPIPBox_3	104	118	PF02747	0.513
LIG_RPA_C_Fungi	661	673	PF08784	0.469
LIG_SH2_CRK	201	205	PF00017	0.473
LIG_SH2_CRK	428	432	PF00017	0.503
LIG_SH2_CRK	466	470	PF00017	0.501
LIG_SH2_CRK	69	73	PF00017	0.415
LIG_SH2_GRB2like	213	216	PF00017	0.506
LIG_SH2_GRB2like	451	454	PF00017	0.376
LIG_SH2_NCK_1	201	205	PF00017	0.397
LIG_SH2_SRC	138	141	PF00017	0.501
LIG_SH2_STAP1	221	225	PF00017	0.399
LIG_SH2_STAP1	236	240	PF00017	0.450
LIG_SH2_STAP1	395	399	PF00017	0.685
LIG_SH2_STAP1	510	514	PF00017	0.430
LIG_SH2_STAT5	142	145	PF00017	0.515
LIG_SH2_STAT5	213	216	PF00017	0.478
LIG_SH2_STAT5	224	227	PF00017	0.358
LIG_SH2_STAT5	236	239	PF00017	0.453
LIG_SH2_STAT5	240	243	PF00017	0.451
LIG_SH2_STAT5	597	600	PF00017	0.410
LIG_SH2_STAT5	623	626	PF00017	0.430
LIG_SH2_STAT5	64	67	PF00017	0.395
LIG_SH2_STAT5	69	72	PF00017	0.404
LIG_SH3_1	26	32	PF00018	0.708
LIG_SH3_1	609	615	PF00018	0.430
LIG_SH3_3	131	137	PF00018	0.404
LIG_SH3_3	22	28	PF00018	0.629
LIG_SH3_3	244	250	PF00018	0.470
LIG_SH3_3	29	35	PF00018	0.578
LIG_SH3_3	349	355	PF00018	0.805
LIG_SH3_3	471	477	PF00018	0.376
LIG_SH3_3	609	615	PF00018	0.376
LIG_SH3_3	75	81	PF00018	0.495
LIG_SH3_4	249	256	PF00018	0.441
LIG_SUMO_SIM_anti_2	131	136	PF11976	0.457
LIG_SUMO_SIM_par_1	342	351	PF11976	0.729
LIG_SUMO_SIM_par_1	356	363	PF11976	0.735
LIG_SUMO_SIM_par_1	647	652	PF11976	0.597
LIG_TRAF2_1	13	16	PF00917	0.789
LIG_TRAF2_1	149	152	PF00917	0.549
LIG_TRAF2_1	274	277	PF00917	0.724
LIG_TRAF2_1	481	484	PF00917	0.462
LIG_TRAF2_1	509	512	PF00917	0.410
LIG_TRAF2_1	82	85	PF00917	0.423
LIG_UBA3_1	574	580	PF00899	0.376
LIG_WRC_WIRS_1	225	230	PF05994	0.399
LIG_WW_3	266	270	PF00397	0.747
MOD_CDK_SPK_2	21	26	PF00069	0.595
MOD_CDK_SPxK_1	348	354	PF00069	0.702
MOD_CDK_SPxK_1	35	41	PF00069	0.621
MOD_CDK_SPxxK_3	452	459	PF00069	0.376
MOD_CDK_SPxxK_3	498	505	PF00069	0.430
MOD_CK1_1	248	254	PF00069	0.543
MOD_CK1_1	375	381	PF00069	0.710
MOD_CK1_1	490	496	PF00069	0.385
MOD_CK2_1	10	16	PF00069	0.710
MOD_CK2_1	179	185	PF00069	0.502
MOD_CK2_1	229	235	PF00069	0.465
MOD_CK2_1	248	254	PF00069	0.365
MOD_CK2_1	271	277	PF00069	0.775
MOD_CK2_1	320	326	PF00069	0.739
MOD_CK2_1	329	335	PF00069	0.700
MOD_CK2_1	368	374	PF00069	0.790
MOD_CK2_1	375	381	PF00069	0.678
MOD_CK2_1	478	484	PF00069	0.453
MOD_CK2_1	498	504	PF00069	0.238
MOD_CK2_1	506	512	PF00069	0.327
MOD_CK2_1	525	531	PF00069	0.376
MOD_CK2_1	79	85	PF00069	0.413
MOD_Cter_Amidation	217	220	PF01082	0.472
MOD_Cter_Amidation	262	265	PF01082	0.719
MOD_Cter_Amidation	532	535	PF01082	0.326
MOD_GlcNHglycan	174	177	PF01048	0.670
MOD_GlcNHglycan	287	290	PF01048	0.794
MOD_GlcNHglycan	330	334	PF01048	0.746
MOD_GlcNHglycan	4	7	PF01048	0.696
MOD_GlcNHglycan	539	542	PF01048	0.491
MOD_GlcNHglycan	625	629	PF01048	0.466
MOD_GSK3_1	285	292	PF00069	0.659
MOD_GSK3_1	368	375	PF00069	0.756
MOD_GSK3_1	457	464	PF00069	0.357
MOD_GSK3_1	468	475	PF00069	0.363
MOD_GSK3_1	512	519	PF00069	0.404
MOD_GSK3_1	91	98	PF00069	0.456
MOD_N-GLC_1	100	105	PF02516	0.525
MOD_N-GLC_1	452	457	PF02516	0.376
MOD_NEK2_1	407	412	PF00069	0.622
MOD_NEK2_1	413	418	PF00069	0.606
MOD_NEK2_1	468	473	PF00069	0.410
MOD_NEK2_1	487	492	PF00069	0.171
MOD_NEK2_1	619	624	PF00069	0.376
MOD_NEK2_2	461	466	PF00069	0.430
MOD_NEK2_2	684	689	PF00069	0.406
MOD_PIKK_1	372	378	PF00454	0.809
MOD_PKA_2	114	120	PF00069	0.459
MOD_PKA_2	289	295	PF00069	0.727
MOD_PKA_2	353	359	PF00069	0.710
MOD_Plk_1	461	467	PF00069	0.501
MOD_Plk_2-3	512	518	PF00069	0.451
MOD_Plk_2-3	79	85	PF00069	0.487
MOD_Plk_4	236	242	PF00069	0.571
MOD_Plk_4	407	413	PF00069	0.567
MOD_Plk_4	569	575	PF00069	0.376
MOD_Plk_4	593	599	PF00069	0.410
MOD_Plk_4	619	625	PF00069	0.376
MOD_ProDKin_1	21	27	PF00069	0.642
MOD_ProDKin_1	320	326	PF00069	0.773
MOD_ProDKin_1	348	354	PF00069	0.741
MOD_ProDKin_1	35	41	PF00069	0.583
MOD_ProDKin_1	452	458	PF00069	0.341
MOD_ProDKin_1	478	484	PF00069	0.430
MOD_ProDKin_1	498	504	PF00069	0.171
MOD_SUMO_rev_2	15	20	PF00179	0.790
MOD_SUMO_rev_2	182	189	PF00179	0.497
TRG_DiLeu_BaEn_1	185	190	PF01217	0.431
TRG_DiLeu_LyEn_5	185	190	PF01217	0.431
TRG_ENDOCYTIC_2	201	204	PF00928	0.417
TRG_ENDOCYTIC_2	42	45	PF00928	0.541
TRG_ENDOCYTIC_2	451	454	PF00928	0.376
TRG_ENDOCYTIC_2	466	469	PF00928	0.376
TRG_ENDOCYTIC_2	623	626	PF00928	0.430
TRG_ENDOCYTIC_2	69	72	PF00928	0.404
TRG_ENDOCYTIC_2	80	83	PF00928	0.443
TRG_ER_diArg_1	219	221	PF00400	0.468
TRG_ER_diArg_1	281	283	PF00400	0.625
TRG_ER_diArg_1	51	54	PF00400	0.564
TRG_ER_diArg_1	607	609	PF00400	0.430
TRG_ER_diArg_1	631	633	PF00400	0.514
TRG_ER_diArg_1	664	666	PF00400	0.504
TRG_NLS_Bipartite_1	281	302	PF00514	0.721
TRG_NLS_MonoExtC_3	49	54	PF00514	0.564
TRG_NLS_MonoExtN_4	281	287	PF00514	0.707
TRG_NLS_MonoExtN_4	297	302	PF00514	0.697
TRG_NLS_MonoExtN_4	51	58	PF00514	0.474
TRG_Pf-PMV_PEXEL_1	188	192	PF00026	0.486
TRG_Pf-PMV_PEXEL_1	439	444	PF00026	0.573
TRG_Pf-PMV_PEXEL_1	89	93	PF00026	0.486

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I609	Leptomonas seymouri	69%	100%
A0A3Q8IKM7	Leishmania donovani	100%	100%
A4H7D9	Leishmania braziliensis	80%	100%
D0A1W0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9B4J9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
Q4Q3H7	Leishmania major	95%	100%