Cysteine protease

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Cysteine protease

Gene product:

AUT2/APG4/ATG4 cysteine peptidase - putative

Species:

Leishmania infantum

UniProt:

A4I8K7_LEIIN

TriTrypDb:

LINF_320046100

Length:

388

Annotations

Annotations by Jardim et al.

Autophagy, AUT2/APG4/ATG4 cysteine peptidase ATG4.1

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	yes	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	11
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

A4I8K7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I8K7

Function

Biological processes
Term	Name	Level	Count
GO:0000045	autophagosome assembly	6	11
GO:0006508	proteolysis	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006810	transport	3	11
GO:0006996	organelle organization	4	11
GO:0007033	vacuole organization	5	11
GO:0008104	protein localization	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0015031	protein transport	4	11
GO:0016043	cellular component organization	3	11
GO:0019538	protein metabolic process	3	11
GO:0022607	cellular component assembly	4	11
GO:0033036	macromolecule localization	2	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044238	primary metabolic process	2	11
GO:0045184	establishment of protein localization	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0051697	protein delipidation	5	11
GO:0070727	cellular macromolecule localization	3	11
GO:0070925	organelle assembly	5	11
GO:0071702	organic substance transport	4	11
GO:0071704	organic substance metabolic process	2	11
GO:0071705	nitrogen compound transport	4	11
GO:0071840	cellular component organization or biogenesis	2	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1905037	autophagosome organization	6	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004175	endopeptidase activity	4	11
GO:0004197	cysteine-type endopeptidase activity	5	11
GO:0008233	peptidase activity	3	11
GO:0008234	cysteine-type peptidase activity	4	11
GO:0016787	hydrolase activity	2	11
GO:0140096	catalytic activity, acting on a protein	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	291	293	PF00675	0.334
CLV_NRD_NRD_1	34	36	PF00675	0.647
CLV_PCSK_KEX2_1	246	248	PF00082	0.301
CLV_PCSK_KEX2_1	291	293	PF00082	0.318
CLV_PCSK_PC1ET2_1	246	248	PF00082	0.360
CLV_PCSK_SKI1_1	227	231	PF00082	0.300
CLV_PCSK_SKI1_1	258	262	PF00082	0.340
CLV_PCSK_SKI1_1	35	39	PF00082	0.570
CLV_PCSK_SKI1_1	61	65	PF00082	0.329
DEG_APCC_DBOX_1	226	234	PF00400	0.536
DEG_ODPH_VHL_1	218	230	PF01847	0.588
DOC_CKS1_1	30	35	PF01111	0.639
DOC_CYCLIN_RxL_1	61	71	PF00134	0.539
DOC_CYCLIN_yCln2_LP_2	210	216	PF00134	0.485
DOC_MAPK_gen_1	126	134	PF00069	0.529
DOC_MAPK_gen_1	291	299	PF00069	0.581
DOC_MAPK_gen_1	35	45	PF00069	0.570
DOC_MAPK_MEF2A_6	292	301	PF00069	0.588
DOC_MAPK_MEF2A_6	38	46	PF00069	0.512
DOC_PP1_RVXF_1	256	263	PF00149	0.565
DOC_PP1_RVXF_1	64	71	PF00149	0.537
DOC_PP2B_LxvP_1	210	213	PF13499	0.485
DOC_PP4_FxxP_1	262	265	PF00568	0.515
DOC_PP4_FxxP_1	30	33	PF00568	0.636
DOC_USP7_MATH_1	271	275	PF00917	0.537
DOC_USP7_MATH_1	377	381	PF00917	0.631
DOC_USP7_MATH_1	80	84	PF00917	0.564
DOC_USP7_UBL2_3	150	154	PF12436	0.588
DOC_USP7_UBL2_3	6	10	PF12436	0.599
DOC_USP7_UBL2_3	61	65	PF12436	0.506
DOC_WW_Pin1_4	157	162	PF00397	0.525
DOC_WW_Pin1_4	29	34	PF00397	0.618
DOC_WW_Pin1_4	336	341	PF00397	0.730
DOC_WW_Pin1_4	363	368	PF00397	0.595
DOC_WW_Pin1_4	38	43	PF00397	0.560
DOC_WW_Pin1_4	381	386	PF00397	0.651
DOC_WW_Pin1_4	8	13	PF00397	0.621
LIG_14-3-3_CanoR_1	247	251	PF00244	0.524
LIG_14-3-3_CanoR_1	94	100	PF00244	0.571
LIG_BIR_II_1	1	5	PF00653	0.680
LIG_BRCT_BRCA1_1	127	131	PF00533	0.508
LIG_BRCT_BRCA1_1	258	262	PF00533	0.485
LIG_BRCT_BRCA1_1	26	30	PF00533	0.607
LIG_EVH1_1	76	80	PF00568	0.588
LIG_FHA_1	110	116	PF00498	0.444
LIG_FHA_1	38	44	PF00498	0.503
LIG_FHA_1	55	61	PF00498	0.351
LIG_FHA_1	62	68	PF00498	0.497
LIG_FHA_1	9	15	PF00498	0.588
LIG_FHA_1	94	100	PF00498	0.520
LIG_FHA_2	303	309	PF00498	0.565
LIG_FHA_2	337	343	PF00498	0.750
LIG_LIR_Apic_2	112	116	PF02991	0.380
LIG_LIR_Apic_2	259	265	PF02991	0.490
LIG_LIR_Apic_2	27	33	PF02991	0.623
LIG_LIR_Gen_1	128	139	PF02991	0.538
LIG_LIR_Gen_1	152	161	PF02991	0.555
LIG_LIR_Gen_1	222	233	PF02991	0.485
LIG_LIR_LC3C_4	314	318	PF02991	0.250
LIG_LIR_Nem_3	128	134	PF02991	0.519
LIG_LIR_Nem_3	307	312	PF02991	0.499
LIG_LIR_Nem_3	86	92	PF02991	0.542
LIG_MLH1_MIPbox_1	258	262	PF16413	0.504
LIG_PTAP_UEV_1	134	139	PF05743	0.485
LIG_SH2_PTP2	113	116	PF00017	0.588
LIG_SH2_STAP1	155	159	PF00017	0.565
LIG_SH2_STAP1	225	229	PF00017	0.499
LIG_SH2_STAT3	107	110	PF00017	0.435
LIG_SH2_STAT5	113	116	PF00017	0.534
LIG_SH2_STAT5	267	270	PF00017	0.536
LIG_SH2_STAT5	272	275	PF00017	0.507
LIG_SH2_STAT5	302	305	PF00017	0.485
LIG_SH2_STAT5	69	72	PF00017	0.491
LIG_SH3_3	132	138	PF00018	0.485
LIG_SH3_3	320	326	PF00018	0.510
LIG_SH3_3	379	385	PF00018	0.609
LIG_SH3_3	74	80	PF00018	0.565
LIG_SUMO_SIM_anti_2	40	48	PF11976	0.546
LIG_SUMO_SIM_par_1	344	353	PF11976	0.801
LIG_SUMO_SIM_par_1	40	48	PF11976	0.512
LIG_UBA3_1	228	236	PF00899	0.585
LIG_WRC_WIRS_1	327	332	PF05994	0.505
LIG_WW_2	77	80	PF00397	0.588
MOD_CDK_SPxK_1	29	35	PF00069	0.644
MOD_CDK_SPxxK_3	29	36	PF00069	0.644
MOD_CK1_1	157	163	PF00069	0.565
MOD_CK1_1	274	280	PF00069	0.519
MOD_CK1_1	363	369	PF00069	0.712
MOD_CK2_1	157	163	PF00069	0.550
MOD_CK2_1	344	350	PF00069	0.742
MOD_GlcNHglycan	135	138	PF01048	0.286
MOD_GlcNHglycan	287	291	PF01048	0.292
MOD_GlcNHglycan	47	50	PF01048	0.424
MOD_GlcNHglycan	51	54	PF01048	0.451
MOD_GSK3_1	270	277	PF00069	0.499
MOD_GSK3_1	344	351	PF00069	0.765
MOD_GSK3_1	377	384	PF00069	0.780
MOD_GSK3_1	45	52	PF00069	0.485
MOD_GSK3_1	80	87	PF00069	0.537
MOD_N-GLC_1	38	43	PF02516	0.532
MOD_N-GLC_1	80	85	PF02516	0.278
MOD_NEK2_1	37	42	PF00069	0.535
MOD_NEK2_1	68	73	PF00069	0.485
MOD_NEK2_1	92	97	PF00069	0.502
MOD_NEK2_1	99	104	PF00069	0.491
MOD_PIKK_1	360	366	PF00454	0.722
MOD_PIKK_1	93	99	PF00454	0.565
MOD_PKA_1	246	252	PF00069	0.521
MOD_PKA_2	125	131	PF00069	0.533
MOD_PKA_2	246	252	PF00069	0.524
MOD_PKA_2	377	383	PF00069	0.728
MOD_PKA_2	93	99	PF00069	0.565
MOD_PKB_1	333	341	PF00069	0.645
MOD_Plk_1	274	280	PF00069	0.435
MOD_Plk_1	360	366	PF00069	0.714
MOD_Plk_4	109	115	PF00069	0.455
MOD_Plk_4	15	21	PF00069	0.477
MOD_Plk_4	154	160	PF00069	0.533
MOD_Plk_4	246	252	PF00069	0.550
MOD_Plk_4	318	324	PF00069	0.462
MOD_ProDKin_1	157	163	PF00069	0.525
MOD_ProDKin_1	29	35	PF00069	0.617
MOD_ProDKin_1	336	342	PF00069	0.734
MOD_ProDKin_1	363	369	PF00069	0.592
MOD_ProDKin_1	38	44	PF00069	0.555
MOD_ProDKin_1	381	387	PF00069	0.646
MOD_ProDKin_1	8	14	PF00069	0.612
TRG_DiLeu_BaLyEn_6	323	328	PF01217	0.413
TRG_ENDOCYTIC_2	155	158	PF00928	0.565
TRG_ENDOCYTIC_2	225	228	PF00928	0.485
TRG_ENDOCYTIC_2	309	312	PF00928	0.588
TRG_ER_diArg_1	291	293	PF00400	0.538
TRG_NES_CRM1_1	122	133	PF08389	0.566
TRG_NLS_MonoExtN_4	33	39	PF00514	0.545
TRG_Pf-PMV_PEXEL_1	191	195	PF00026	0.388

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A098DRK7	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	22%	83%
A0A0N0P8C0	Leptomonas seymouri	25%	97%
A0A0N1IIY5	Leptomonas seymouri	59%	100%
A0A0S4JSC7	Bodo saltans	26%	100%
A0A1X0NS04	Trypanosomatidae	37%	100%
A0A1X0P2H1	Trypanosomatidae	25%	100%
A0A3S7X6B3	Leishmania donovani	99%	100%
A0A422NI42	Trypanosoma rangeli	38%	100%
A1CJ08	Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)	25%	97%
A2QY50	Aspergillus niger (strain CBS 513.88 / FGSC A1513)	25%	96%
A2XHJ5	Oryza sativa subsp. indica	23%	82%
A3LQU0	Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)	28%	75%
A4HHX1	Leishmania braziliensis	25%	100%
A4HL26	Leishmania braziliensis	77%	100%
A5DEF7	Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)	27%	97%
A5DSB4	Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239)	23%	74%
A7TQN1	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	24%	94%
D0AAK8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
E2RDP2	Canis lupus familiaris	24%	82%
E9B3G7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
Q1E5M9	Coccidioides immitis (strain RS)	25%	90%
Q2HH40	Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970)	25%	87%
Q2U5B0	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	26%	95%
Q4Q4N3	Leishmania major	88%	100%
Q4U3V5	Cryphonectria parasitica	26%	85%
Q523C3	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	25%	79%
Q59UG3	Candida albicans (strain SC5314 / ATCC MYA-2876)	24%	87%
Q5B7L0	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	23%	97%
Q5XH30	Xenopus laevis	25%	86%
Q684M2	Sus scrofa	23%	83%
Q68EP9	Xenopus tropicalis	23%	86%
Q68FJ9	Xenopus laevis	24%	83%
Q6CH28	Yarrowia lipolytica (strain CLIB 122 / E 150)	25%	71%
Q6FP20	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	26%	80%
Q75E61	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	26%	74%
Q75KP8	Oryza sativa subsp. japonica	22%	82%
Q811C2	Mus musculus	25%	85%
Q86TL0	Homo sapiens	24%	82%
Q8BGV9	Mus musculus	24%	82%
Q8S929	Arabidopsis thaliana	25%	83%
Q96DT6	Homo sapiens	25%	85%
Q9M1Y0	Arabidopsis thaliana	23%	81%
V5AXZ8	Trypanosoma cruzi	37%	100%