Tubulin-tyrosine ligase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tubulin-tyrosine ligase-like protein

Species:

Leishmania infantum

UniProt:

A4I8B4_LEIIN

TriTrypDb:

LINF_320036500

Length:

495

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005874	microtubule	6	1
GO:0005876	spindle microtubule	7	1
GO:0099080	supramolecular complex	2	1
GO:0099081	supramolecular polymer	3	1
GO:0099512	supramolecular fiber	4	1
GO:0099513	polymeric cytoskeletal fiber	5	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4I8B4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I8B4

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008152	metabolic process	1	10
GO:0019538	protein metabolic process	3	10
GO:0036211	protein modification process	4	10
GO:0043170	macromolecule metabolic process	3	10
GO:0043412	macromolecule modification	4	10
GO:0044238	primary metabolic process	2	10
GO:0071704	organic substance metabolic process	2	10
GO:1901564	organonitrogen compound metabolic process	3	10
GO:0000226	microtubule cytoskeleton organization	3	1
GO:0006996	organelle organization	4	1
GO:0007010	cytoskeleton organization	5	1
GO:0007017	microtubule-based process	2	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0004835	tubulin-tyrosine ligase activity	3	7
GO:0016874	ligase activity	2	10
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	7
GO:0016881	acid-amino acid ligase activity	4	7
GO:0140096	catalytic activity, acting on a protein	2	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	313	317	PF00656	0.484
CLV_NRD_NRD_1	239	241	PF00675	0.242
CLV_NRD_NRD_1	66	68	PF00675	0.406
CLV_NRD_NRD_1	84	86	PF00675	0.582
CLV_PCSK_KEX2_1	239	241	PF00082	0.283
CLV_PCSK_KEX2_1	485	487	PF00082	0.483
CLV_PCSK_KEX2_1	66	68	PF00082	0.403
CLV_PCSK_KEX2_1	71	73	PF00082	0.447
CLV_PCSK_KEX2_1	84	86	PF00082	0.582
CLV_PCSK_PC1ET2_1	239	241	PF00082	0.344
CLV_PCSK_PC1ET2_1	485	487	PF00082	0.511
CLV_PCSK_PC1ET2_1	71	73	PF00082	0.540
CLV_PCSK_PC7_1	67	73	PF00082	0.515
CLV_PCSK_SKI1_1	120	124	PF00082	0.371
CLV_PCSK_SKI1_1	13	17	PF00082	0.456
CLV_PCSK_SKI1_1	207	211	PF00082	0.317
CLV_PCSK_SKI1_1	233	237	PF00082	0.284
CLV_PCSK_SKI1_1	326	330	PF00082	0.293
CLV_PCSK_SKI1_1	460	464	PF00082	0.459
DEG_APCC_DBOX_1	206	214	PF00400	0.496
DEG_APCC_DBOX_1	311	319	PF00400	0.484
DEG_APCC_DBOX_1	361	369	PF00400	0.441
DEG_Nend_UBRbox_1	1	4	PF02207	0.370
DEG_SCF_FBW7_1	153	158	PF00400	0.457
DOC_CYCLIN_RxL_1	10	18	PF00134	0.494
DOC_CYCLIN_RxL_1	207	218	PF00134	0.528
DOC_CYCLIN_yClb5_NLxxxL_5	437	446	PF00134	0.333
DOC_MAPK_gen_1	483	493	PF00069	0.471
DOC_MAPK_MEF2A_6	465	472	PF00069	0.443
DOC_MAPK_MEF2A_6	91	100	PF00069	0.639
DOC_MAPK_NFAT4_5	465	473	PF00069	0.444
DOC_PP1_RVXF_1	109	116	PF00149	0.486
DOC_PP1_RVXF_1	209	216	PF00149	0.525
DOC_PP1_SILK_1	207	212	PF00149	0.488
DOC_PP2B_LxvP_1	98	101	PF13499	0.534
DOC_USP7_MATH_1	101	105	PF00917	0.391
DOC_USP7_MATH_1	155	159	PF00917	0.558
DOC_USP7_MATH_1	188	192	PF00917	0.467
DOC_USP7_MATH_1	371	375	PF00917	0.426
DOC_USP7_MATH_1	412	416	PF00917	0.469
DOC_USP7_MATH_1	87	91	PF00917	0.584
DOC_WW_Pin1_4	151	156	PF00397	0.457
DOC_WW_Pin1_4	278	283	PF00397	0.550
DOC_WW_Pin1_4	333	338	PF00397	0.546
DOC_WW_Pin1_4	92	97	PF00397	0.605
LIG_14-3-3_CanoR_1	120	125	PF00244	0.371
LIG_14-3-3_CanoR_1	20	26	PF00244	0.470
LIG_14-3-3_CanoR_1	266	271	PF00244	0.442
LIG_14-3-3_CanoR_1	27	32	PF00244	0.543
LIG_14-3-3_CanoR_1	381	386	PF00244	0.517
LIG_14-3-3_CanoR_1	91	96	PF00244	0.586
LIG_Actin_WH2_2	109	127	PF00022	0.385
LIG_APCC_ABBA_1	257	262	PF00400	0.484
LIG_APCC_ABBA_1	383	388	PF00400	0.402
LIG_CaM_NSCaTE_8	445	452	PF13499	0.400
LIG_eIF4E_1	230	236	PF01652	0.442
LIG_eIF4E_1	258	264	PF01652	0.459
LIG_FHA_1	117	123	PF00498	0.426
LIG_FHA_1	128	134	PF00498	0.525
LIG_FHA_1	265	271	PF00498	0.490
LIG_FHA_1	281	287	PF00498	0.500
LIG_FHA_1	30	36	PF00498	0.486
LIG_FHA_1	389	395	PF00498	0.477
LIG_FHA_1	426	432	PF00498	0.471
LIG_FHA_1	465	471	PF00498	0.394
LIG_FHA_1	477	483	PF00498	0.491
LIG_FHA_2	38	44	PF00498	0.614
LIG_Integrin_RGD_1	203	205	PF01839	0.257
LIG_Integrin_RGD_1	348	350	PF01839	0.257
LIG_LIR_Apic_2	90	96	PF02991	0.562
LIG_LIR_Gen_1	18	26	PF02991	0.400
LIG_LIR_Gen_1	426	437	PF02991	0.387
LIG_LIR_Gen_1	6	16	PF02991	0.378
LIG_LIR_LC3C_4	32	37	PF02991	0.540
LIG_LIR_Nem_3	18	24	PF02991	0.438
LIG_LIR_Nem_3	205	209	PF02991	0.488
LIG_LIR_Nem_3	253	258	PF02991	0.469
LIG_LIR_Nem_3	298	304	PF02991	0.474
LIG_LIR_Nem_3	333	338	PF02991	0.457
LIG_LIR_Nem_3	426	432	PF02991	0.403
LIG_Pex14_2	432	436	PF04695	0.359
LIG_SH2_CRK	206	210	PF00017	0.488
LIG_SH2_CRK	335	339	PF00017	0.457
LIG_SH2_CRK	434	438	PF00017	0.302
LIG_SH2_CRK	93	97	PF00017	0.734
LIG_SH2_GRB2like	357	360	PF00017	0.457
LIG_SH2_NCK_1	93	97	PF00017	0.547
LIG_SH2_PTP2	490	493	PF00017	0.381
LIG_SH2_SRC	304	307	PF00017	0.484
LIG_SH2_STAP1	255	259	PF00017	0.442
LIG_SH2_STAP1	434	438	PF00017	0.299
LIG_SH2_STAP1	8	12	PF00017	0.391
LIG_SH2_STAT5	146	149	PF00017	0.459
LIG_SH2_STAT5	230	233	PF00017	0.442
LIG_SH2_STAT5	258	261	PF00017	0.459
LIG_SH2_STAT5	335	338	PF00017	0.584
LIG_SH2_STAT5	475	478	PF00017	0.605
LIG_SH2_STAT5	490	493	PF00017	0.201
LIG_SH2_STAT5	93	96	PF00017	0.757
LIG_SH3_2	343	348	PF14604	0.457
LIG_SH3_2	453	458	PF14604	0.500
LIG_SH3_3	228	234	PF00018	0.525
LIG_SH3_3	340	346	PF00018	0.510
LIG_SH3_3	348	354	PF00018	0.510
LIG_SH3_3	415	421	PF00018	0.523
LIG_SH3_3	450	456	PF00018	0.482
LIG_SH3_3	471	477	PF00018	0.371
LIG_SUMO_SIM_par_1	381	388	PF11976	0.523
LIG_SUMO_SIM_par_1	390	395	PF11976	0.509
LIG_TRFH_1	151	155	PF08558	0.525
MOD_CDK_SPK_2	337	342	PF00069	0.510
MOD_CDK_SPxxK_3	278	285	PF00069	0.488
MOD_CDK_SPxxK_3	92	99	PF00069	0.594
MOD_CK1_1	127	133	PF00069	0.448
MOD_CK1_1	22	28	PF00069	0.671
MOD_CK1_1	276	282	PF00069	0.505
MOD_CK1_1	358	364	PF00069	0.524
MOD_CK1_1	374	380	PF00069	0.403
MOD_CK1_1	92	98	PF00069	0.730
MOD_CK2_1	156	162	PF00069	0.589
MOD_CK2_1	299	305	PF00069	0.462
MOD_CK2_1	3	9	PF00069	0.404
MOD_GlcNHglycan	103	106	PF01048	0.464
MOD_GlcNHglycan	190	193	PF01048	0.284
MOD_GlcNHglycan	275	278	PF01048	0.300
MOD_GSK3_1	116	123	PF00069	0.352
MOD_GSK3_1	147	154	PF00069	0.457
MOD_GSK3_1	15	22	PF00069	0.540
MOD_GSK3_1	215	222	PF00069	0.469
MOD_GSK3_1	276	283	PF00069	0.554
MOD_GSK3_1	295	302	PF00069	0.477
MOD_GSK3_1	333	340	PF00069	0.591
MOD_GSK3_1	371	378	PF00069	0.579
MOD_GSK3_1	83	90	PF00069	0.645
MOD_N-GLC_1	358	363	PF02516	0.264
MOD_N-GLC_1	371	376	PF02516	0.219
MOD_N-GLC_2	291	293	PF02516	0.242
MOD_NEK2_1	124	129	PF00069	0.448
MOD_NEK2_1	15	20	PF00069	0.492
MOD_NEK2_1	166	171	PF00069	0.459
MOD_NEK2_1	215	220	PF00069	0.502
MOD_NEK2_1	265	270	PF00069	0.480
MOD_NEK2_1	44	49	PF00069	0.553
MOD_NEK2_2	321	326	PF00069	0.488
MOD_PIKK_1	215	221	PF00454	0.493
MOD_PIKK_1	37	43	PF00454	0.593
MOD_PK_1	266	272	PF00069	0.442
MOD_PK_1	27	33	PF00069	0.547
MOD_PK_1	381	387	PF00069	0.441
MOD_PKA_2	19	25	PF00069	0.483
MOD_PKA_2	265	271	PF00069	0.442
MOD_PKA_2	273	279	PF00069	0.442
MOD_PKA_2	355	361	PF00069	0.559
MOD_PKA_2	406	412	PF00069	0.520
MOD_PKA_2	83	89	PF00069	0.622
MOD_Plk_4	124	130	PF00069	0.479
MOD_Plk_4	299	305	PF00069	0.489
MOD_Plk_4	3	9	PF00069	0.461
MOD_Plk_4	361	367	PF00069	0.451
MOD_Plk_4	406	412	PF00069	0.508
MOD_ProDKin_1	151	157	PF00069	0.457
MOD_ProDKin_1	278	284	PF00069	0.550
MOD_ProDKin_1	333	339	PF00069	0.546
MOD_ProDKin_1	92	98	PF00069	0.597
MOD_SUMO_for_1	330	333	PF00179	0.540
TRG_DiLeu_BaEn_1	162	167	PF01217	0.484
TRG_DiLeu_BaEn_4	305	311	PF01217	0.459
TRG_DiLeu_BaLyEn_6	231	236	PF01217	0.442
TRG_ENDOCYTIC_2	146	149	PF00928	0.504
TRG_ENDOCYTIC_2	206	209	PF00928	0.488
TRG_ENDOCYTIC_2	335	338	PF00928	0.457
TRG_ENDOCYTIC_2	434	437	PF00928	0.334
TRG_ENDOCYTIC_2	490	493	PF00928	0.346
TRG_ENDOCYTIC_2	8	11	PF00928	0.377
TRG_ER_diArg_1	66	68	PF00400	0.405
TRG_NES_CRM1_1	164	179	PF08389	0.418

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8M9	Leptomonas seymouri	69%	77%
A0A1X0NRN8	Trypanosomatidae	41%	100%
A0A3Q8IH06	Leishmania donovani	100%	78%
A0A422NE52	Trypanosoma rangeli	28%	100%
A0A422NZN7	Trypanosoma rangeli	40%	96%
A4HKT5	Leishmania braziliensis	86%	100%
E9B373	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
Q4Q4Y1	Leishmania major	96%	100%