Putative 1-acyl-sn-glycerol-3-phosphateacyltransferase-lik e protein

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

1-acyl-sn-glycerol-3-phosphateacyltransferase-like protein - putative

Species:

Leishmania infantum

UniProt:

A4I816_LEIIN

TriTrypDb:

LINF_320025500

Length:

277

Annotations

LeishMANIAdb annotations

This conserved lipid acyltransferase has many re-entrant segments but only one true TM helix. Most closely related to bacterial acyltransferases.. Heavily expanded in kinetoplastids for unknown reasons. Localization: ER (by homology)

Annotations by Jardim et al.

Phospholipid biosynthesis, 1-acyl-sn-glycerol-3-phosphateacyltransferase-lik e

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	13
GO:0110165	cellular anatomical entity	1	13

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A4I816

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I816

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	1
GO:0006644	phospholipid metabolic process	4	1
GO:0006650	glycerophospholipid metabolic process	5	1
GO:0006654	phosphatidic acid biosynthetic process	6	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0008152	metabolic process	1	1
GO:0008610	lipid biosynthetic process	4	1
GO:0008654	phospholipid biosynthetic process	5	1
GO:0009058	biosynthetic process	2	1
GO:0009987	cellular process	1	1
GO:0019637	organophosphate metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044255	cellular lipid metabolic process	3	1
GO:0045017	glycerolipid biosynthetic process	4	1
GO:0046473	phosphatidic acid metabolic process	6	1
GO:0046474	glycerophospholipid biosynthetic process	5	1
GO:0046486	glycerolipid metabolic process	4	1
GO:0071704	organic substance metabolic process	2	1
GO:0090407	organophosphate biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	16
GO:0003841	1-acylglycerol-3-phosphate O-acyltransferase activity	7	8
GO:0008374	O-acyltransferase activity	5	8
GO:0016411	acylglycerol O-acyltransferase activity	6	8
GO:0016740	transferase activity	2	16
GO:0016746	acyltransferase activity	3	16
GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups	4	8
GO:0042171	lysophosphatidic acid acyltransferase activity	6	8
GO:0071617	lysophospholipid acyltransferase activity	5	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	226	230	PF00656	0.289
CLV_NRD_NRD_1	164	166	PF00675	0.385
CLV_NRD_NRD_1	167	169	PF00675	0.372
CLV_NRD_NRD_1	4	6	PF00675	0.294
CLV_NRD_NRD_1	58	60	PF00675	0.479
CLV_PCSK_FUR_1	165	169	PF00082	0.396
CLV_PCSK_KEX2_1	163	165	PF00082	0.395
CLV_PCSK_KEX2_1	167	169	PF00082	0.361
CLV_PCSK_KEX2_1	4	6	PF00082	0.294
CLV_PCSK_PC7_1	163	169	PF00082	0.392
CLV_PCSK_SKI1_1	241	245	PF00082	0.444
CLV_PCSK_SKI1_1	273	277	PF00082	0.446
CLV_PCSK_SKI1_1	88	92	PF00082	0.345
DEG_SPOP_SBC_1	43	47	PF00917	0.327
DOC_CYCLIN_yClb1_LxF_4	190	195	PF00134	0.192
DOC_MAPK_gen_1	163	174	PF00069	0.173
DOC_MAPK_gen_1	261	270	PF00069	0.334
DOC_MAPK_gen_1	85	93	PF00069	0.209
DOC_MAPK_MEF2A_6	12	21	PF00069	0.398
DOC_MAPK_MEF2A_6	85	93	PF00069	0.176
DOC_PP1_RVXF_1	190	196	PF00149	0.178
DOC_PP1_RVXF_1	57	64	PF00149	0.252
DOC_PP4_FxxP_1	122	125	PF00568	0.168
DOC_PP4_FxxP_1	234	237	PF00568	0.221
DOC_USP7_MATH_1	104	108	PF00917	0.259
DOC_USP7_MATH_1	219	223	PF00917	0.294
LIG_14-3-3_CanoR_1	12	18	PF00244	0.378
LIG_14-3-3_CanoR_1	241	249	PF00244	0.280
LIG_14-3-3_CanoR_1	59	64	PF00244	0.230
LIG_Actin_WH2_2	46	61	PF00022	0.175
LIG_AP2alpha_2	100	102	PF02296	0.180
LIG_APCC_ABBA_1	172	177	PF00400	0.171
LIG_BIR_II_1	1	5	PF00653	0.590
LIG_BRCT_BRCA1_1	40	44	PF00533	0.316
LIG_deltaCOP1_diTrp_1	112	122	PF00928	0.168
LIG_EH_1	188	192	PF12763	0.273
LIG_EH_1	69	73	PF12763	0.211
LIG_EH1_1	17	25	PF00400	0.199
LIG_eIF4E_1	18	24	PF01652	0.347
LIG_FHA_1	14	20	PF00498	0.190
LIG_FHA_1	212	218	PF00498	0.276
LIG_FHA_2	43	49	PF00498	0.286
LIG_LIR_Apic_2	233	237	PF02991	0.217
LIG_LIR_Gen_1	100	110	PF02991	0.212
LIG_LIR_Gen_1	146	155	PF02991	0.272
LIG_LIR_Gen_1	16	25	PF02991	0.243
LIG_LIR_Nem_3	100	105	PF02991	0.213
LIG_LIR_Nem_3	146	151	PF02991	0.272
LIG_LIR_Nem_3	16	21	PF02991	0.246
LIG_LIR_Nem_3	229	234	PF02991	0.257
LIG_Pex14_2	122	126	PF04695	0.189
LIG_Pex14_2	191	195	PF04695	0.162
LIG_SH2_STAT5	18	21	PF00017	0.232
LIG_SH2_STAT5	231	234	PF00017	0.203
LIG_SH3_2	187	192	PF14604	0.273
LIG_SH3_3	181	187	PF00018	0.218
LIG_SH3_3	37	43	PF00018	0.298
LIG_SUMO_SIM_par_1	207	212	PF11976	0.204
LIG_TRAF2_1	245	248	PF00917	0.239
LIG_WRC_WIRS_1	231	236	PF05994	0.305
MOD_CK1_1	147	153	PF00069	0.281
MOD_CK1_1	242	248	PF00069	0.327
MOD_CK2_1	209	215	PF00069	0.244
MOD_CK2_1	242	248	PF00069	0.254
MOD_CK2_1	42	48	PF00069	0.325
MOD_GlcNHglycan	211	214	PF01048	0.394
MOD_GlcNHglycan	221	224	PF01048	0.445
MOD_GlcNHglycan	244	247	PF01048	0.543
MOD_GlcNHglycan	96	99	PF01048	0.379
MOD_GSK3_1	143	150	PF00069	0.168
MOD_GSK3_1	38	45	PF00069	0.414
MOD_NEK2_1	13	18	PF00069	0.248
MOD_NEK2_1	19	24	PF00069	0.261
MOD_NEK2_1	209	214	PF00069	0.261
MOD_NEK2_2	71	76	PF00069	0.199
MOD_PIKK_1	247	253	PF00454	0.220
MOD_PKA_1	144	150	PF00069	0.240
MOD_PKA_1	59	65	PF00069	0.240
MOD_PKA_2	219	225	PF00069	0.340
MOD_Plk_4	111	117	PF00069	0.183
MOD_Plk_4	13	19	PF00069	0.263
MOD_Plk_4	202	208	PF00069	0.197
MOD_Plk_4	59	65	PF00069	0.286
TRG_DiLeu_BaLyEn_6	9	14	PF01217	0.457
TRG_ENDOCYTIC_2	18	21	PF00928	0.242
TRG_ENDOCYTIC_2	231	234	PF00928	0.273
TRG_ER_diArg_1	163	165	PF00400	0.175
TRG_ER_diArg_1	166	168	PF00400	0.165

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P455	Leptomonas seymouri	29%	81%
A0A0N0P6B0	Leptomonas seymouri	80%	96%
A0A0S4IQ05	Bodo saltans	29%	82%
A0A0S4JRP7	Bodo saltans	50%	100%
A0A1X0NUQ7	Trypanosomatidae	58%	100%
A0A3Q8IFY7	Leishmania donovani	100%	100%
A0A3S5IQW0	Trypanosoma rangeli	59%	100%
A4H420	Leishmania braziliensis	30%	78%
A4HKI5	Leishmania braziliensis	88%	100%
A4HSA1	Leishmania infantum	24%	100%
A4I4U1	Leishmania infantum	24%	100%
D0AA81	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
E9AE89	Leishmania major	24%	100%
E9AK86	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	90%
E9ALJ6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9B2X4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
Q42670	Cocos nucifera	23%	90%
Q4Q582	Leishmania major	94%	100%
Q59188	Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)	26%	100%
Q9LLY4	Brassica napus	27%	81%
V5ALB6	Trypanosoma cruzi	58%	100%