Uncharacterized protein

Quick info Annotations Localization Phosphorylation Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein phosphatase 2C - putative

Species:

Leishmania infantum

UniProt:

A4I7Y4_LEIIN

TriTrypDb:

LINF_320022600

Length:

563

Annotations

LeishMANIAdb annotations

This large family encompasses many diverse protein phosphatases. Some appear to have evolved transmembrane segments. Very tentatively they might regulate transmembrane receptor kinases.. The TM and non-TM groups diverged early in Eukaryota and appear to be distinct enough that they probably should not be part of the same cluster. This latter group has not expanded.

Annotations by Jardim et al.

Phosphatase, phosphatase 2C Uncharacterized

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 1
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 1
Pissara et al.	no	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 12

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 22
NetGPI	no	yes: 0, no: 22

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	5
GO:0110165	cellular anatomical entity	1	8
GO:0005783	endoplasmic reticulum	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0005737	cytoplasm	2	3
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	2
GO:0099568	cytoplasmic region	3	2
GO:0005654	nucleoplasm	2	1

Phosphorylation

Promastigote: 548

Expansion

Sequence features

A4I7Y4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I7Y4

Function

Biological processes
Term	Name	Level	Count
GO:0006470	protein dephosphorylation	5	16
GO:0006793	phosphorus metabolic process	3	16
GO:0006796	phosphate-containing compound metabolic process	4	16
GO:0006807	nitrogen compound metabolic process	2	16
GO:0008152	metabolic process	1	16
GO:0009987	cellular process	1	16
GO:0016311	dephosphorylation	5	16
GO:0019538	protein metabolic process	3	16
GO:0036211	protein modification process	4	16
GO:0043170	macromolecule metabolic process	3	16
GO:0043412	macromolecule modification	4	16
GO:0044237	cellular metabolic process	2	16
GO:0044238	primary metabolic process	2	16
GO:0071704	organic substance metabolic process	2	16
GO:1901564	organonitrogen compound metabolic process	3	16
GO:0035970	peptidyl-threonine dephosphorylation	6	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	23
GO:0004721	phosphoprotein phosphatase activity	3	23
GO:0004722	protein serine/threonine phosphatase activity	4	23
GO:0016787	hydrolase activity	2	23
GO:0016788	hydrolase activity, acting on ester bonds	3	23
GO:0016791	phosphatase activity	5	23
GO:0042578	phosphoric ester hydrolase activity	4	23
GO:0140096	catalytic activity, acting on a protein	2	23
GO:0005488	binding	1	6
GO:0043167	ion binding	2	6
GO:0043169	cation binding	3	6
GO:0046872	metal ion binding	4	6
GO:0017018	myosin phosphatase activity	5	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	366	370	PF00656	0.275
CLV_C14_Caspase3-7	519	523	PF00656	0.606
CLV_NRD_NRD_1	10	12	PF00675	0.536
CLV_NRD_NRD_1	112	114	PF00675	0.357
CLV_NRD_NRD_1	145	147	PF00675	0.213
CLV_NRD_NRD_1	281	283	PF00675	0.220
CLV_NRD_NRD_1	45	47	PF00675	0.414
CLV_PCSK_FUR_1	43	47	PF00082	0.374
CLV_PCSK_KEX2_1	10	12	PF00082	0.554
CLV_PCSK_KEX2_1	112	114	PF00082	0.361
CLV_PCSK_KEX2_1	145	147	PF00082	0.213
CLV_PCSK_KEX2_1	281	283	PF00082	0.220
CLV_PCSK_KEX2_1	392	394	PF00082	0.396
CLV_PCSK_KEX2_1	45	47	PF00082	0.373
CLV_PCSK_PC1ET2_1	392	394	PF00082	0.396
CLV_PCSK_SKI1_1	261	265	PF00082	0.398
CLV_PCSK_SKI1_1	504	508	PF00082	0.526
CLV_PCSK_SKI1_1	51	55	PF00082	0.298
CLV_Separin_Metazoa	109	113	PF03568	0.374
DEG_APCC_DBOX_1	356	364	PF00400	0.251
DEG_APCC_DBOX_1	50	58	PF00400	0.301
DOC_CKS1_1	451	456	PF01111	0.499
DOC_MAPK_gen_1	281	289	PF00069	0.213
DOC_MAPK_gen_1	351	360	PF00069	0.238
DOC_MAPK_MEF2A_6	51	59	PF00069	0.537
DOC_PP2B_LxvP_1	6	9	PF13499	0.399
DOC_PP4_FxxP_1	349	352	PF00568	0.251
DOC_PP4_MxPP_1	412	415	PF00568	0.453
DOC_USP7_MATH_1	17	21	PF00917	0.449
DOC_USP7_MATH_1	240	244	PF00917	0.213
DOC_USP7_MATH_1	302	306	PF00917	0.311
DOC_USP7_MATH_1	310	314	PF00917	0.313
DOC_USP7_MATH_1	418	422	PF00917	0.552
DOC_USP7_MATH_1	441	445	PF00917	0.555
DOC_USP7_MATH_1	468	472	PF00917	0.610
DOC_USP7_MATH_1	491	495	PF00917	0.742
DOC_USP7_MATH_1	502	506	PF00917	0.522
DOC_USP7_MATH_1	510	514	PF00917	0.550
DOC_USP7_MATH_1	523	527	PF00917	0.528
DOC_USP7_MATH_1	538	542	PF00917	0.513
DOC_USP7_MATH_1	549	553	PF00917	0.521
DOC_USP7_UBL2_3	190	194	PF12436	0.222
DOC_WW_Pin1_4	370	375	PF00397	0.246
DOC_WW_Pin1_4	406	411	PF00397	0.569
DOC_WW_Pin1_4	450	455	PF00397	0.559
DOC_WW_Pin1_4	483	488	PF00397	0.583
DOC_WW_Pin1_4	545	550	PF00397	0.547
LIG_14-3-3_CanoR_1	145	149	PF00244	0.300
LIG_14-3-3_CanoR_1	45	49	PF00244	0.372
LIG_APCC_ABBA_1	226	231	PF00400	0.251
LIG_Clathr_ClatBox_1	154	158	PF01394	0.251
LIG_DCNL_PONY_1	1	4	PF03556	0.451
LIG_EH1_1	47	55	PF00400	0.289
LIG_FHA_1	151	157	PF00498	0.254
LIG_FHA_1	45	51	PF00498	0.529
LIG_FHA_1	506	512	PF00498	0.653
LIG_FHA_1	56	62	PF00498	0.393
LIG_FHA_1	75	81	PF00498	0.317
LIG_LIR_Apic_2	348	352	PF02991	0.251
LIG_LIR_Gen_1	180	191	PF02991	0.262
LIG_LIR_Gen_1	203	212	PF02991	0.267
LIG_LIR_Gen_1	327	337	PF02991	0.317
LIG_LIR_Nem_3	130	136	PF02991	0.305
LIG_LIR_Nem_3	180	186	PF02991	0.251
LIG_LIR_Nem_3	203	208	PF02991	0.267
LIG_LIR_Nem_3	327	332	PF02991	0.317
LIG_LIR_Nem_3	63	68	PF02991	0.325
LIG_LIR_Nem_3	69	73	PF02991	0.328
LIG_PCNA_yPIPBox_3	273	287	PF02747	0.251
LIG_PCNA_yPIPBox_3	31	45	PF02747	0.389
LIG_SH2_CRK	70	74	PF00017	0.333
LIG_SH2_SRC	211	214	PF00017	0.245
LIG_SH2_SRC	229	232	PF00017	0.175
LIG_SH2_STAT3	181	184	PF00017	0.251
LIG_SH2_STAT3	26	29	PF00017	0.375
LIG_SH2_STAT5	177	180	PF00017	0.356
LIG_SH2_STAT5	211	214	PF00017	0.257
LIG_SH2_STAT5	229	232	PF00017	0.304
LIG_SH2_STAT5	236	239	PF00017	0.219
LIG_SH2_STAT5	26	29	PF00017	0.398
LIG_SH2_STAT5	277	280	PF00017	0.215
LIG_SH2_STAT5	48	51	PF00017	0.345
LIG_SH3_3	259	265	PF00018	0.256
LIG_SH3_3	412	418	PF00018	0.752
LIG_SH3_3	543	549	PF00018	0.516
LIG_SH3_3	6	12	PF00018	0.446
LIG_SUMO_SIM_anti_2	153	158	PF11976	0.244
LIG_SUMO_SIM_anti_2	385	390	PF11976	0.299
LIG_SUMO_SIM_anti_2	58	63	PF11976	0.269
LIG_SUMO_SIM_par_1	152	158	PF11976	0.251
LIG_SUMO_SIM_par_1	285	290	PF11976	0.251
LIG_SUMO_SIM_par_1	57	63	PF11976	0.444
LIG_SUMO_SIM_par_1	72	77	PF11976	0.257
LIG_UBA3_1	1	5	PF00899	0.452
LIG_UBA3_1	154	159	PF00899	0.251
LIG_WRC_WIRS_1	215	220	PF05994	0.238
LIG_WW_3	413	417	PF00397	0.442
LIG_WW_3	7	11	PF00397	0.438
MOD_CDK_SPK_2	487	492	PF00069	0.515
MOD_CK1_1	214	220	PF00069	0.282
MOD_CK1_1	429	435	PF00069	0.529
MOD_CK1_1	444	450	PF00069	0.473
MOD_CK1_1	500	506	PF00069	0.592
MOD_CK1_1	513	519	PF00069	0.494
MOD_CK1_1	533	539	PF00069	0.495
MOD_CK2_1	310	316	PF00069	0.296
MOD_GlcNHglycan	127	130	PF01048	0.371
MOD_GlcNHglycan	169	172	PF01048	0.259
MOD_GlcNHglycan	19	22	PF01048	0.385
MOD_GlcNHglycan	312	315	PF01048	0.318
MOD_GlcNHglycan	420	423	PF01048	0.529
MOD_GlcNHglycan	431	434	PF01048	0.502
MOD_GlcNHglycan	470	473	PF01048	0.748
MOD_GlcNHglycan	494	497	PF01048	0.586
MOD_GlcNHglycan	504	507	PF01048	0.681
MOD_GlcNHglycan	518	521	PF01048	0.503
MOD_GlcNHglycan	536	539	PF01048	0.520
MOD_GSK3_1	167	174	PF00069	0.307
MOD_GSK3_1	398	405	PF00069	0.535
MOD_GSK3_1	427	434	PF00069	0.535
MOD_GSK3_1	453	460	PF00069	0.613
MOD_GSK3_1	483	490	PF00069	0.693
MOD_GSK3_1	492	499	PF00069	0.531
MOD_GSK3_1	512	519	PF00069	0.542
MOD_GSK3_1	529	536	PF00069	0.605
MOD_GSK3_1	545	552	PF00069	0.747
MOD_N-GLC_1	249	254	PF02516	0.222
MOD_N-GLC_1	382	387	PF02516	0.357
MOD_N-GLC_1	434	439	PF02516	0.552
MOD_N-GLC_1	533	538	PF02516	0.517
MOD_NEK2_1	287	292	PF00069	0.320
MOD_NEK2_1	511	516	PF00069	0.776
MOD_NEK2_1	55	60	PF00069	0.292
MOD_NEK2_2	538	543	PF00069	0.513
MOD_PIKK_1	25	31	PF00454	0.364
MOD_PK_1	249	255	PF00069	0.222
MOD_PKA_2	144	150	PF00069	0.359
MOD_PKA_2	317	323	PF00069	0.254
MOD_PKA_2	44	50	PF00069	0.424
MOD_PKA_2	523	529	PF00069	0.552
MOD_Plk_1	382	388	PF00069	0.357
MOD_Plk_1	426	432	PF00069	0.643
MOD_Plk_4	150	156	PF00069	0.277
MOD_Plk_4	382	388	PF00069	0.290
MOD_Plk_4	57	63	PF00069	0.295
MOD_Plk_4	74	80	PF00069	0.456
MOD_ProDKin_1	370	376	PF00069	0.246
MOD_ProDKin_1	406	412	PF00069	0.568
MOD_ProDKin_1	450	456	PF00069	0.561
MOD_ProDKin_1	483	489	PF00069	0.583
MOD_ProDKin_1	545	551	PF00069	0.545
MOD_SUMO_for_1	193	196	PF00179	0.229
MOD_SUMO_rev_2	254	263	PF00179	0.302
MOD_SUMO_rev_2	476	486	PF00179	0.561
TRG_ENDOCYTIC_2	202	205	PF00928	0.216
TRG_ENDOCYTIC_2	70	73	PF00928	0.337
TRG_ER_diArg_1	111	113	PF00400	0.338
TRG_ER_diArg_1	144	146	PF00400	0.213
TRG_ER_diArg_1	281	283	PF00400	0.226
TRG_ER_diArg_1	350	353	PF00400	0.213
TRG_ER_diArg_1	9	11	PF00400	0.486
TRG_Pf-PMV_PEXEL_1	291	295	PF00026	0.213
TRG_Pf-PMV_PEXEL_1	36	40	PF00026	0.431

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PET0	Leptomonas seymouri	73%	97%
A0A3Q8IFG7	Leishmania donovani	30%	100%
A0A3Q8IGS1	Leishmania donovani	100%	100%
A0A3Q8IL63	Leishmania donovani	29%	100%
A0A3S7WTA2	Leishmania donovani	36%	100%
A0A3S7WZ14	Leishmania donovani	32%	100%
A4H7Y6	Leishmania braziliensis	36%	100%
A4HAW5	Leishmania braziliensis	30%	100%
A4HE10	Leishmania braziliensis	31%	100%
A4HHY5	Leishmania braziliensis	28%	100%
A4HKF6	Leishmania braziliensis	78%	100%
A4HWB4	Leishmania infantum	36%	100%
A4I1B7	Leishmania infantum	32%	100%
A4I565	Leishmania infantum	31%	100%
A4IA25	Leishmania infantum	29%	100%
D0AA51	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
E9AQ14	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9AXF3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9B0G2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9B2U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
E9B540	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
Q4Q2U6	Leishmania major	29%	100%
Q4Q5B1	Leishmania major	92%	100%
Q4Q7S1	Leishmania major	31%	100%
Q4QA19	Leishmania major	32%	100%
Q4QFG7	Leishmania major	35%	100%
Q6K5I0	Oryza sativa subsp. japonica	30%	100%
Q9CAJ0	Arabidopsis thaliana	29%	100%