Lem3 cdc50 family protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

LEM3 (ligand-effect modulator 3) family / CDC50 family - putative

Species:

Leishmania infantum

UniProt:

A4I7N1_LEIIN

TriTrypDb:

LINF_320010400

Length:

363

Annotations

LeishMANIAdb annotations

Homologous to animal CDC50 family phospholipid flippase (ATPase) proteins.. This family has expanded in kinetoplastids

Annotations by Jardim et al.

Transcription, LEM3 (ligand-effect modulator 3) family / CDC50 family, putative

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 16
NetGPI	no	yes: 0, no: 16

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	17
GO:0110165	cellular anatomical entity	1	17
GO:0005783	endoplasmic reticulum	5	1
GO:0005794	Golgi apparatus	5	1
GO:0005886	plasma membrane	3	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A4I7N1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I7N1

Function

Biological processes
Term	Name	Level	Count
GO:0033036	macromolecule localization	2	17
GO:0051179	localization	1	17

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_SKI1_1	124	128	PF00082	0.509
CLV_PCSK_SKI1_1	207	211	PF00082	0.530
CLV_PCSK_SKI1_1	242	246	PF00082	0.417
CLV_PCSK_SKI1_1	97	101	PF00082	0.485
DOC_CYCLIN_yCln2_LP_2	106	112	PF00134	0.253
DOC_MAPK_FxFP_2	149	152	PF00069	0.291
DOC_MAPK_gen_1	284	293	PF00069	0.308
DOC_MAPK_MEF2A_6	25	33	PF00069	0.445
DOC_MAPK_MEF2A_6	284	293	PF00069	0.304
DOC_PP1_RVXF_1	56	62	PF00149	0.325
DOC_PP1_RVXF_1	95	101	PF00149	0.313
DOC_PP2B_LxvP_1	106	109	PF13499	0.280
DOC_PP2B_LxvP_1	356	359	PF13499	0.460
DOC_PP4_FxxP_1	149	152	PF00568	0.270
DOC_USP7_MATH_1	214	218	PF00917	0.309
DOC_USP7_MATH_1	277	281	PF00917	0.327
DOC_USP7_MATH_1	310	314	PF00917	0.344
DOC_USP7_MATH_1	320	324	PF00917	0.325
DOC_USP7_UBL2_3	7	11	PF12436	0.661
DOC_WW_Pin1_4	114	119	PF00397	0.307
DOC_WW_Pin1_4	24	29	PF00397	0.559
LIG_14-3-3_CanoR_1	124	132	PF00244	0.315
LIG_14-3-3_CanoR_1	138	145	PF00244	0.292
LIG_14-3-3_CanoR_1	97	103	PF00244	0.296
LIG_APCC_ABBA_1	358	363	PF00400	0.492
LIG_BIR_II_1	1	5	PF00653	0.634
LIG_BRCT_BRCA1_1	116	120	PF00533	0.245
LIG_BRCT_BRCA1_1	31	35	PF00533	0.420
LIG_BRCT_BRCA1_1	312	316	PF00533	0.336
LIG_BRCT_BRCA1_1	67	71	PF00533	0.410
LIG_FHA_1	105	111	PF00498	0.287
LIG_FHA_1	125	131	PF00498	0.106
LIG_FHA_1	277	283	PF00498	0.287
LIG_FHA_1	340	346	PF00498	0.394
LIG_FHA_1	54	60	PF00498	0.392
LIG_FHA_2	55	61	PF00498	0.384
LIG_LIR_Apic_2	161	166	PF02991	0.325
LIG_LIR_Apic_2	221	226	PF02991	0.248
LIG_LIR_Gen_1	129	139	PF02991	0.317
LIG_LIR_Gen_1	313	320	PF02991	0.312
LIG_LIR_Gen_1	352	361	PF02991	0.459
LIG_LIR_Gen_1	60	71	PF02991	0.345
LIG_LIR_Nem_3	117	123	PF02991	0.306
LIG_LIR_Nem_3	129	135	PF02991	0.324
LIG_LIR_Nem_3	283	289	PF02991	0.251
LIG_LIR_Nem_3	297	302	PF02991	0.263
LIG_LIR_Nem_3	313	319	PF02991	0.213
LIG_LIR_Nem_3	32	38	PF02991	0.387
LIG_LIR_Nem_3	60	66	PF02991	0.307
LIG_MLH1_MIPbox_1	116	120	PF16413	0.190
LIG_NRBOX	331	337	PF00104	0.428
LIG_Pex14_2	79	83	PF04695	0.190
LIG_PTAP_UEV_1	306	311	PF05743	0.287
LIG_PTB_Apo_2	119	126	PF02174	0.342
LIG_PTB_Phospho_1	119	125	PF10480	0.287
LIG_SH2_CRK	132	136	PF00017	0.297
LIG_SH2_CRK	223	227	PF00017	0.315
LIG_SH2_CRK	286	290	PF00017	0.280
LIG_SH2_GRB2like	163	166	PF00017	0.342
LIG_SH2_NCK_1	63	67	PF00017	0.336
LIG_SH2_SRC	63	66	PF00017	0.307
LIG_SH2_STAT5	125	128	PF00017	0.297
LIG_SH2_STAT5	248	251	PF00017	0.297
LIG_SH2_STAT5	299	302	PF00017	0.247
LIG_SH2_STAT5	355	358	PF00017	0.531
LIG_SH3_3	2	8	PF00018	0.667
LIG_SH3_3	288	294	PF00018	0.319
LIG_SH3_3	304	310	PF00018	0.319
LIG_SH3_3	78	84	PF00018	0.336
LIG_SH3_4	7	14	PF00018	0.671
LIG_SH3_5	294	298	PF00018	0.319
LIG_SUMO_SIM_anti_2	30	35	PF11976	0.354
LIG_SUMO_SIM_anti_2	334	340	PF11976	0.454
LIG_SUMO_SIM_par_1	262	269	PF11976	0.190
LIG_SUMO_SIM_par_1	27	32	PF11976	0.540
LIG_SUMO_SIM_par_1	46	52	PF11976	0.158
LIG_TRFH_1	114	118	PF08558	0.420
LIG_TYR_ITIM	130	135	PF00017	0.448
LIG_WW_3	224	228	PF00397	0.339
MOD_CK1_1	133	139	PF00069	0.383
MOD_CK1_1	140	146	PF00069	0.389
MOD_CK1_1	193	199	PF00069	0.380
MOD_CK1_1	27	33	PF00069	0.437
MOD_CK1_1	280	286	PF00069	0.313
MOD_CK2_1	262	268	PF00069	0.339
MOD_GlcNHglycan	135	138	PF01048	0.345
MOD_GlcNHglycan	139	142	PF01048	0.328
MOD_GlcNHglycan	160	163	PF01048	0.445
MOD_GlcNHglycan	191	195	PF01048	0.369
MOD_GlcNHglycan	210	213	PF01048	0.377
MOD_GlcNHglycan	236	239	PF01048	0.387
MOD_GlcNHglycan	242	245	PF01048	0.365
MOD_GlcNHglycan	51	54	PF01048	0.487
MOD_GlcNHglycan	91	94	PF01048	0.415
MOD_GSK3_1	126	133	PF00069	0.307
MOD_GSK3_1	177	184	PF00069	0.306
MOD_GSK3_1	193	200	PF00069	0.366
MOD_GSK3_1	276	283	PF00069	0.409
MOD_GSK3_1	49	56	PF00069	0.478
MOD_GSK3_1	85	92	PF00069	0.329
MOD_GSK3_1	98	105	PF00069	0.299
MOD_N-GLC_1	175	180	PF02516	0.284
MOD_N-GLC_1	197	202	PF02516	0.373
MOD_N-GLC_1	240	245	PF02516	0.400
MOD_N-GLC_1	85	90	PF02516	0.305
MOD_N-GLC_2	206	208	PF02516	0.292
MOD_NEK2_1	175	180	PF00069	0.286
MOD_NEK2_1	29	34	PF00069	0.404
MOD_NEK2_1	339	344	PF00069	0.299
MOD_NEK2_1	346	351	PF00069	0.296
MOD_NEK2_1	85	90	PF00069	0.341
MOD_NEK2_1	98	103	PF00069	0.316
MOD_PIKK_1	130	136	PF00454	0.318
MOD_PKA_2	137	143	PF00069	0.366
MOD_PKA_2	214	220	PF00069	0.428
MOD_Plk_1	130	136	PF00069	0.376
MOD_Plk_1	175	181	PF00069	0.276
MOD_Plk_4	184	190	PF00069	0.374
MOD_Plk_4	29	35	PF00069	0.328
MOD_Plk_4	331	337	PF00069	0.387
MOD_Plk_4	346	352	PF00069	0.144
MOD_Plk_4	85	91	PF00069	0.430
MOD_ProDKin_1	114	120	PF00069	0.387
MOD_ProDKin_1	24	30	PF00069	0.456
TRG_ENDOCYTIC_2	123	126	PF00928	0.355
TRG_ENDOCYTIC_2	132	135	PF00928	0.370
TRG_ENDOCYTIC_2	286	289	PF00928	0.314
TRG_ENDOCYTIC_2	299	302	PF00928	0.273
TRG_ENDOCYTIC_2	355	358	PF00928	0.482
TRG_ENDOCYTIC_2	63	66	PF00928	0.365
TRG_Pf-PMV_PEXEL_1	287	292	PF00026	0.359

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PBG2	Leptomonas seymouri	31%	87%
A0A0N1PDQ9	Leptomonas seymouri	60%	100%
A0A0S4IMK4	Bodo saltans	31%	90%
A0A0S4J4T1	Bodo saltans	40%	96%
A0A0S4JB98	Bodo saltans	31%	90%
A0A0S4JJY0	Bodo saltans	29%	90%
A0A0S4JQR9	Bodo saltans	33%	69%
A0A1X0NHQ5	Trypanosomatidae	31%	76%
A0A1X0NV71	Trypanosomatidae	40%	96%
A0A1X0P5L3	Trypanosomatidae	32%	91%
A0A3R7LIN7	Trypanosoma rangeli	31%	85%
A0A3R7MGX3	Trypanosoma rangeli	41%	97%
A0A3S7X551	Leishmania donovani	100%	100%
A0A3S7X9N4	Leishmania donovani	30%	86%
A0A422NR22	Trypanosoma rangeli	34%	91%
A4HK31	Leishmania braziliensis	73%	100%
A4HN07	Leishmania braziliensis	30%	100%
A4IBN0	Leishmania infantum	30%	86%
C9ZZ02	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	94%
D0A9K3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	77%
D0A9R9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9AFG4	Leishmania major	31%	100%
E9B2H4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
E9B6L9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
P25656	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	93%
P42838	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	88%
P53740	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	92%
Q17QL5	Bos taurus	31%	100%
Q1MTQ5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	92%
Q2T9P5	Bos taurus	25%	100%
Q3MIR4	Homo sapiens	28%	100%
Q4Q5N2	Leishmania major	91%	100%
Q5F362	Gallus gallus	31%	98%
Q5R6C0	Pongo abelii	30%	100%
Q67YS6	Arabidopsis thaliana	26%	100%
Q6AY41	Rattus norvegicus	30%	100%
Q8BHG3	Mus musculus	27%	100%
Q8L8W0	Arabidopsis thaliana	27%	100%
Q8VEK0	Mus musculus	31%	100%
Q95JK4	Macaca fascicularis	26%	100%
Q96WW4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	98%
Q9D4D7	Mus musculus	25%	100%
Q9LTW0	Arabidopsis thaliana	28%	100%
Q9NV96	Homo sapiens	30%	100%
Q9SA35	Arabidopsis thaliana	28%	100%
Q9SLK2	Arabidopsis thaliana	27%	100%
V5AY40	Trypanosoma cruzi	34%	100%
V5B6A6	Trypanosoma cruzi	42%	96%
V5BY23	Trypanosoma cruzi	31%	91%