Glycylpeptide N-tetradecanoyltransferase

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

N-myristoyl transferase - putative

Species:

Leishmania infantum

UniProt:

A4I7H1_LEIIN

TriTrypDb:

LINF_320005800

Length:

421

Annotations

Annotations by Jardim et al.

Glycosylation, Glycylpeptide N-tetradecanoyltransferase NMT

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	yes	yes: 3
Silverman et al.	no	yes: 3
Pissara et al.	yes	yes: 12

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A4I7H1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I7H1

PDB structure(s): 2wsa_A , 3h5z_A , 4a2z_A , 4a30_A , 4a31_A , 4a32_A , 4a33_A , 4c7h_A , 4c7i_A , 4cgl_A , 4cgm_A , 4cgn_A , 4cgo_A , 4cgp_A , 4cyn_A , 4cyo_A , 4cyp_A , 4cyq_A , 4ucm_A , 4ucn_A , 4ucp_A , 5a27_A , 5a28_A , 5ag4_A , 5ag5_A , 5ag6_A , 5ag7_A , 5age_A , 5g20_A , 5g21_A , 6eu5_A , 6ewf_A , 6gnh_A , 6gns_A , 6gnt_A , 6gnu_A , 6gnv_A , 6qd9_A , 6qda_A , 6qdb_A , 6qdc_A , 6qdd_A , 6qde_A , 6qdf_A , 6qdg_A , 6qdh_A , 6tw7_AAA , 6tw8_AAA , 6tw8_CCC

Function

Biological processes
Term	Name	Level	Count
GO:0006497	protein lipidation	5	12
GO:0006498	N-terminal protein lipidation	6	12
GO:0006499	N-terminal protein myristoylation	7	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0018377	protein myristoylation	6	12
GO:0019538	protein metabolic process	3	12
GO:0031365	N-terminal protein amino acid modification	5	12
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043543	protein acylation	5	12
GO:0044238	primary metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:0018008	N-terminal peptidyl-glycine N-myristoylation	7	1
GO:0018193	peptidyl-amino acid modification	5	1
GO:0018201	peptidyl-glycine modification	6	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004379	glycylpeptide N-tetradecanoyltransferase activity	6	12
GO:0016410	N-acyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016746	acyltransferase activity	3	12
GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups	4	12
GO:0019107	myristoyltransferase activity	5	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	379	383	PF00656	0.373
CLV_C14_Caspase3-7	6	10	PF00656	0.679
CLV_MEL_PAP_1	228	234	PF00089	0.349
CLV_NRD_NRD_1	115	117	PF00675	0.307
CLV_NRD_NRD_1	190	192	PF00675	0.311
CLV_NRD_NRD_1	276	278	PF00675	0.381
CLV_PCSK_FUR_1	176	180	PF00082	0.326
CLV_PCSK_KEX2_1	115	117	PF00082	0.307
CLV_PCSK_KEX2_1	178	180	PF00082	0.305
CLV_PCSK_KEX2_1	190	192	PF00082	0.280
CLV_PCSK_KEX2_1	250	252	PF00082	0.307
CLV_PCSK_KEX2_1	276	278	PF00082	0.385
CLV_PCSK_PC1ET2_1	178	180	PF00082	0.355
CLV_PCSK_PC1ET2_1	250	252	PF00082	0.307
CLV_PCSK_SKI1_1	120	124	PF00082	0.365
CLV_PCSK_SKI1_1	139	143	PF00082	0.308
CLV_PCSK_SKI1_1	186	190	PF00082	0.292
CLV_PCSK_SKI1_1	265	269	PF00082	0.399
CLV_PCSK_SKI1_1	276	280	PF00082	0.181
CLV_PCSK_SKI1_1	29	33	PF00082	0.486
CLV_PCSK_SKI1_1	317	321	PF00082	0.335
CLV_Separin_Metazoa	74	78	PF03568	0.268
DEG_SCF_FBW7_1	207	214	PF00400	0.307
DOC_CKS1_1	135	140	PF01111	0.326
DOC_CKS1_1	208	213	PF01111	0.292
DOC_CKS1_1	58	63	PF01111	0.377
DOC_MAPK_DCC_7	176	185	PF00069	0.307
DOC_MAPK_gen_1	115	125	PF00069	0.293
DOC_MAPK_gen_1	176	185	PF00069	0.292
DOC_MAPK_gen_1	410	418	PF00069	0.469
DOC_MAPK_MEF2A_6	412	420	PF00069	0.465
DOC_PP1_RVXF_1	119	126	PF00149	0.439
DOC_PP1_RVXF_1	27	33	PF00149	0.524
DOC_PP2B_LxvP_1	101	104	PF13499	0.292
DOC_PP4_FxxP_1	242	245	PF00568	0.292
DOC_PP4_FxxP_1	32	35	PF00568	0.504
DOC_USP7_MATH_1	211	215	PF00917	0.412
DOC_USP7_MATH_1	259	263	PF00917	0.412
DOC_USP7_MATH_1	350	354	PF00917	0.349
DOC_USP7_MATH_1	5	9	PF00917	0.628
DOC_USP7_MATH_1	51	55	PF00917	0.319
DOC_USP7_UBL2_3	117	121	PF12436	0.439
DOC_USP7_UBL2_3	144	148	PF12436	0.391
DOC_WW_Pin1_4	134	139	PF00397	0.326
DOC_WW_Pin1_4	207	212	PF00397	0.292
DOC_WW_Pin1_4	57	62	PF00397	0.300
LIG_14-3-3_CanoR_1	327	333	PF00244	0.408
LIG_14-3-3_CanoR_1	89	99	PF00244	0.326
LIG_Actin_WH2_2	119	136	PF00022	0.439
LIG_BIR_II_1	1	5	PF00653	0.635
LIG_BRCT_BRCA1_1	368	372	PF00533	0.397
LIG_BRCT_BRCA1_1	92	96	PF00533	0.307
LIG_CSL_BTD_1	58	61	PF09270	0.292
LIG_EH1_1	371	379	PF00400	0.429
LIG_eIF4E_1	202	208	PF01652	0.326
LIG_FHA_1	15	21	PF00498	0.382
LIG_FHA_1	187	193	PF00498	0.292
LIG_FHA_1	201	207	PF00498	0.292
LIG_FHA_1	25	31	PF00498	0.421
LIG_FHA_1	308	314	PF00498	0.366
LIG_FHA_2	21	27	PF00498	0.502
LIG_FHA_2	39	45	PF00498	0.156
LIG_LIR_Apic_2	298	303	PF02991	0.361
LIG_LIR_Apic_2	324	329	PF02991	0.278
LIG_LIR_Apic_2	55	61	PF02991	0.298
LIG_LIR_Gen_1	137	145	PF02991	0.361
LIG_LIR_Gen_1	165	175	PF02991	0.292
LIG_LIR_Gen_1	195	206	PF02991	0.378
LIG_LIR_Gen_1	336	346	PF02991	0.383
LIG_LIR_Nem_3	137	143	PF02991	0.361
LIG_LIR_Nem_3	165	171	PF02991	0.292
LIG_LIR_Nem_3	195	201	PF02991	0.350
LIG_LIR_Nem_3	229	235	PF02991	0.326
LIG_LIR_Nem_3	320	326	PF02991	0.283
LIG_LIR_Nem_3	336	341	PF02991	0.256
LIG_LYPXL_yS_3	408	411	PF13949	0.292
LIG_PDZ_Class_2	416	421	PF00595	0.318
LIG_Pex14_1	198	202	PF04695	0.278
LIG_PTB_Apo_2	332	339	PF02174	0.314
LIG_PTB_Apo_2	380	387	PF02174	0.303
LIG_PTB_Apo_2	402	409	PF02174	0.278
LIG_PTB_Phospho_1	332	338	PF10480	0.314
LIG_PTB_Phospho_1	339	345	PF10480	0.299
LIG_PTB_Phospho_1	402	408	PF10480	0.292
LIG_SH2_CRK	140	144	PF00017	0.361
LIG_SH2_CRK	157	161	PF00017	0.386
LIG_SH2_CRK	253	257	PF00017	0.377
LIG_SH2_CRK	326	330	PF00017	0.330
LIG_SH2_CRK	338	342	PF00017	0.266
LIG_SH2_STAP1	92	96	PF00017	0.292
LIG_SH2_STAT5	106	109	PF00017	0.377
LIG_SH2_STAT5	202	205	PF00017	0.292
LIG_SH2_STAT5	217	220	PF00017	0.346
LIG_SH2_STAT5	282	285	PF00017	0.412
LIG_SH2_STAT5	300	303	PF00017	0.307
LIG_SH2_STAT5	311	314	PF00017	0.307
LIG_SH2_STAT5	345	348	PF00017	0.292
LIG_SH2_STAT5	402	405	PF00017	0.309
LIG_SH2_STAT5	80	83	PF00017	0.278
LIG_SH3_1	253	259	PF00018	0.326
LIG_SH3_3	203	209	PF00018	0.439
LIG_SH3_3	253	259	PF00018	0.307
LIG_SH3_3	43	49	PF00018	0.294
LIG_SUMO_SIM_anti_2	362	367	PF11976	0.332
LIG_SUMO_SIM_anti_2	414	420	PF11976	0.434
LIG_SUMO_SIM_par_1	181	187	PF11976	0.292
LIG_SUMO_SIM_par_1	376	382	PF11976	0.349
LIG_SxIP_EBH_1	231	241	PF03271	0.352
LIG_WRC_WIRS_1	322	327	PF05994	0.292
LIG_WW_1	103	106	PF00397	0.292
MOD_CDK_SPK_2	134	139	PF00069	0.326
MOD_CK2_1	20	26	PF00069	0.507
MOD_CK2_1	290	296	PF00069	0.349
MOD_CK2_1	382	388	PF00069	0.377
MOD_CK2_1	51	57	PF00069	0.316
MOD_GlcNHglycan	213	216	PF01048	0.442
MOD_GlcNHglycan	232	235	PF01048	0.142
MOD_GlcNHglycan	261	264	PF01048	0.405
MOD_GSK3_1	20	27	PF00069	0.501
MOD_GSK3_1	207	214	PF00069	0.294
MOD_GSK3_1	317	324	PF00069	0.306
MOD_N-GLC_1	192	197	PF02516	0.412
MOD_N-GLC_1	382	387	PF02516	0.377
MOD_N-GLC_1	90	95	PF02516	0.307
MOD_NEK2_1	14	19	PF00069	0.391
MOD_NEK2_1	218	223	PF00069	0.355
MOD_PIKK_1	14	20	PF00454	0.394
MOD_PIKK_1	411	417	PF00454	0.393
MOD_PKA_1	317	323	PF00069	0.377
MOD_PKA_2	230	236	PF00069	0.349
MOD_Plk_1	295	301	PF00069	0.361
MOD_Plk_1	382	388	PF00069	0.450
MOD_Plk_1	83	89	PF00069	0.349
MOD_Plk_2-3	38	44	PF00069	0.402
MOD_Plk_2-3	382	388	PF00069	0.412
MOD_Plk_4	102	108	PF00069	0.379
MOD_Plk_4	328	334	PF00069	0.287
MOD_Plk_4	83	89	PF00069	0.292
MOD_ProDKin_1	134	140	PF00069	0.326
MOD_ProDKin_1	207	213	PF00069	0.292
MOD_ProDKin_1	57	63	PF00069	0.300
MOD_SUMO_rev_2	262	270	PF00179	0.422
MOD_SUMO_rev_2	314	320	PF00179	0.439
MOD_SUMO_rev_2	37	42	PF00179	0.420
TRG_ENDOCYTIC_2	140	143	PF00928	0.361
TRG_ENDOCYTIC_2	239	242	PF00928	0.292
TRG_ENDOCYTIC_2	338	341	PF00928	0.326
TRG_ENDOCYTIC_2	408	411	PF00928	0.286
TRG_ENDOCYTIC_2	73	76	PF00928	0.297
TRG_ER_diArg_1	114	116	PF00400	0.307
TRG_ER_diArg_1	190	192	PF00400	0.314
TRG_ER_diArg_1	275	277	PF00400	0.412
TRG_Pf-PMV_PEXEL_1	161	165	PF00026	0.326
TRG_Pf-PMV_PEXEL_1	173	177	PF00026	0.377
TRG_Pf-PMV_PEXEL_1	220	225	PF00026	0.357

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8K2	Leptomonas seymouri	78%	100%
A0A0S4JHM4	Bodo saltans	60%	100%
A0A1X0P7N7	Trypanosomatidae	59%	92%
A0A3R7MKK1	Trypanosoma rangeli	55%	94%
A0A3S5H7R0	Leishmania donovani	100%	100%
A4HJY8	Leishmania braziliensis	91%	100%
A7YT82	Danio rerio	43%	86%
D0A003	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	94%
E9B2C8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
O43010	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	42%	90%
O60551	Homo sapiens	44%	85%
O61613	Drosophila melanogaster	41%	89%
O70310	Mus musculus	42%	85%
O70311	Mus musculus	45%	80%
O74234	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	41%	93%
P0CP20	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	43%	85%
P0CP21	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	43%	85%
P14743	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	41%	93%
P30418	Candida albicans (strain SC5314 / ATCC MYA-2876)	43%	93%
P30419	Homo sapiens	42%	85%
P31717	Bos taurus	42%	85%
P34763	Ajellomyces capsulatus	40%	80%
P34809	Cryptococcus neoformans	44%	86%
P46548	Caenorhabditis elegans	43%	94%
Q4I061	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	40%	75%
Q4Q5S8	Leishmania major	98%	100%
Q553B6	Dictyostelium discoideum	44%	100%
Q5RAF3	Pongo abelii	42%	85%
Q5UR64	Acanthamoeba polyphaga mimivirus	27%	100%
Q6BJF4	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	43%	93%
Q6C7G2	Yarrowia lipolytica (strain CLIB 122 / E 150)	41%	95%
Q6CMK4	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	41%	94%
Q75EK2	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	40%	93%
Q7S3C8	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	41%	74%
Q8ILW6	Plasmodium falciparum (isolate 3D7)	40%	100%
Q8K1Q0	Rattus norvegicus	42%	85%
Q8TFN1	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	41%	85%
Q94L32	Arabidopsis thaliana	38%	98%
Q9LTR9	Arabidopsis thaliana	47%	97%
Q9N181	Bos taurus	44%	85%
Q9U419	Plasmodium falciparum	40%	100%
Q9UVX3	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	41%	86%
V5DTE0	Trypanosoma cruzi	56%	93%