Uncharacterized protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

emp24/gp25L/p24 family/GOLD - putative

Species:

Leishmania infantum

UniProt:

A4I6N6_LEIIN

TriTrypDb:

LINF_310017500

Length:

209

Annotations

LeishMANIAdb annotations

Related to other eukaryotic COPII adaptors. Likely involved in ER to Golgi transport.. A Leishmaniid-specific subgroup of these proteins is missing the SigP (unclear topology and function). Localization: ER (by homology) / Golgi (by homology)

Annotations by Jardim et al.

Transporters, Uncharacterized

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 5

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 15, no: 9
NetGPI	no	yes: 0, no: 24

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	25
GO:0110165	cellular anatomical entity	1	25
GO:0005783	endoplasmic reticulum	5	2
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	2
GO:0005794	Golgi apparatus	5	2
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	2
GO:0030135	coated vesicle	7	2
GO:0031410	cytoplasmic vesicle	6	2
GO:0031982	vesicle	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097708	intracellular vesicle	5	2

Expansion

Sequence features

A4I6N6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I6N6

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0006886	intracellular protein transport	4	2
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	2
GO:0006996	organelle organization	4	2
GO:0007030	Golgi organization	5	2
GO:0008104	protein localization	4	2
GO:0009987	cellular process	1	2
GO:0015031	protein transport	4	2
GO:0016043	cellular component organization	3	2
GO:0016192	vesicle-mediated transport	4	2
GO:0033036	macromolecule localization	2	2
GO:0045184	establishment of protein localization	3	2
GO:0046907	intracellular transport	3	2
GO:0048193	Golgi vesicle transport	5	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0070727	cellular macromolecule localization	3	2
GO:0071702	organic substance transport	4	2
GO:0071705	nitrogen compound transport	4	2
GO:0071840	cellular component organization or biogenesis	2	2

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	130	132	PF00675	0.345
CLV_NRD_NRD_1	152	154	PF00675	0.484
CLV_NRD_NRD_1	206	208	PF00675	0.351
CLV_PCSK_KEX2_1	2	4	PF00082	0.627
CLV_PCSK_KEX2_1	205	207	PF00082	0.370
CLV_PCSK_PC1ET2_1	2	4	PF00082	0.653
CLV_PCSK_PC1ET2_1	205	207	PF00082	0.351
DEG_Nend_UBRbox_1	1	4	PF02207	0.599
DOC_CYCLIN_RxL_1	95	105	PF00134	0.326
DOC_MAPK_FxFP_2	63	66	PF00069	0.208
DOC_MAPK_gen_1	2	10	PF00069	0.551
DOC_MAPK_MEF2A_6	3	12	PF00069	0.443
DOC_PP1_RVXF_1	174	180	PF00149	0.258
DOC_PP1_RVXF_1	96	103	PF00149	0.276
DOC_PP2B_LxvP_1	89	92	PF13499	0.316
DOC_PP4_FxxP_1	63	66	PF00568	0.372
DOC_WW_Pin1_4	81	86	PF00397	0.191
LIG_14-3-3_CterR_2	206	209	PF00244	0.619
LIG_BRCT_BRCA1_1	114	118	PF00533	0.238
LIG_EH1_1	40	48	PF00400	0.168
LIG_FHA_1	125	131	PF00498	0.274
LIG_FHA_1	42	48	PF00498	0.271
LIG_FHA_1	72	78	PF00498	0.287
LIG_FHA_1	95	101	PF00498	0.293
LIG_FHA_2	142	148	PF00498	0.185
LIG_LIR_Apic_2	62	66	PF02991	0.348
LIG_LIR_Gen_1	144	152	PF02991	0.305
LIG_PTB_Apo_2	57	64	PF02174	0.371
LIG_SH2_GRB2like	58	61	PF00017	0.332
LIG_SH2_GRB2like	88	91	PF00017	0.191
LIG_SH2_STAT5	88	91	PF00017	0.281
LIG_SUMO_SIM_anti_2	9	14	PF11976	0.433
LIG_SUMO_SIM_par_1	109	115	PF11976	0.179
LIG_SUMO_SIM_par_1	6	11	PF11976	0.440
LIG_UBA3_1	145	154	PF00899	0.179
LIG_WRC_WIRS_1	185	190	PF05994	0.277
LIG_WRC_WIRS_1	199	204	PF05994	0.399
MOD_CK1_1	109	115	PF00069	0.266
MOD_CK2_1	141	147	PF00069	0.278
MOD_CK2_1	198	204	PF00069	0.379
MOD_GlcNHglycan	28	31	PF01048	0.653
MOD_GSK3_1	35	42	PF00069	0.339
MOD_GSK3_1	90	97	PF00069	0.320
MOD_NEK2_1	104	109	PF00069	0.273
MOD_NEK2_1	111	116	PF00069	0.277
MOD_NEK2_1	198	203	PF00069	0.533
MOD_NEK2_1	20	25	PF00069	0.425
MOD_NEK2_1	8	13	PF00069	0.413
MOD_PIKK_1	104	110	PF00454	0.208
MOD_PKA_2	71	77	PF00069	0.287
MOD_Plk_2-3	39	45	PF00069	0.252
MOD_Plk_4	106	112	PF00069	0.304
MOD_Plk_4	141	147	PF00069	0.270
MOD_Plk_4	184	190	PF00069	0.304
MOD_Plk_4	8	14	PF00069	0.344
MOD_ProDKin_1	81	87	PF00069	0.191
MOD_SUMO_rev_2	133	142	PF00179	0.297
TRG_ENDOCYTIC_2	88	91	PF00928	0.270
TRG_ER_diArg_1	67	70	PF00400	0.191
TRG_Pf-PMV_PEXEL_1	121	125	PF00026	0.391
TRG_Pf-PMV_PEXEL_1	153	157	PF00026	0.558
TRG_Pf-PMV_PEXEL_1	167	171	PF00026	0.356
TRG_Pf-PMV_PEXEL_1	98	103	PF00026	0.540

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZG2	Leptomonas seymouri	27%	96%
A0A0N1I2E2	Leptomonas seymouri	39%	98%
A0A0N1II77	Leptomonas seymouri	43%	100%
A0A0N1PA81	Leptomonas seymouri	42%	100%
A0A0S4JG68	Bodo saltans	25%	95%
A0A0S4JHA6	Bodo saltans	26%	100%
A0A1X0NKZ3	Trypanosomatidae	27%	95%
A0A1X0NVX2	Trypanosomatidae	25%	92%
A0A3Q8ITC4	Leishmania donovani	32%	100%
A0A3R7KJS3	Trypanosoma rangeli	31%	96%
A0A3S7X4G7	Leishmania donovani	40%	97%
A4HJ91	Leishmania braziliensis	41%	100%
A4HJ92	Leishmania braziliensis	58%	100%
A4I6N5	Leishmania infantum	41%	100%
A4I824	Leishmania infantum	32%	100%
B4JG34	Drosophila grimshawi	23%	97%
B4KB41	Drosophila mojavensis	22%	97%
B4LYB8	Drosophila virilis	21%	97%
B4NKL0	Drosophila willistoni	21%	97%
C9ZM72	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
C9ZWV3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
E9B1Q8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9B1Q9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
E9B2Y2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
Q4Q6F1	Leishmania major	88%	100%
Q4Q6F2	Leishmania major	41%	100%
V5BK13	Trypanosoma cruzi	29%	95%