Afi1 domain-containing protein

Quick info Localization Phosphorylation Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Stabilization of polarity axis - putative

Species:

Leishmania infantum

UniProt:

A4I5V0_LEIIN

TriTrypDb:

LINF_300032500

Length:

943

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005886	plasma membrane	3	1
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Phosphorylation

Amastigote: 371

Expansion

Sequence features

A4I5V0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I5V0

Function

Biological processes
Term	Name	Level	Count
GO:0009987	cellular process	1	1
GO:0051179	localization	1	1
GO:0051641	cellular localization	2	1
GO:0051666	actin cortical patch localization	3	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	410	414	PF00656	0.678
CLV_C14_Caspase3-7	460	464	PF00656	0.573
CLV_C14_Caspase3-7	822	826	PF00656	0.411
CLV_NRD_NRD_1	1	3	PF00675	0.567
CLV_NRD_NRD_1	120	122	PF00675	0.569
CLV_NRD_NRD_1	280	282	PF00675	0.459
CLV_NRD_NRD_1	327	329	PF00675	0.783
CLV_NRD_NRD_1	467	469	PF00675	0.525
CLV_NRD_NRD_1	618	620	PF00675	0.295
CLV_NRD_NRD_1	646	648	PF00675	0.314
CLV_NRD_NRD_1	703	705	PF00675	0.483
CLV_NRD_NRD_1	879	881	PF00675	0.365
CLV_PCSK_KEX2_1	120	122	PF00082	0.569
CLV_PCSK_KEX2_1	461	463	PF00082	0.466
CLV_PCSK_KEX2_1	467	469	PF00082	0.525
CLV_PCSK_KEX2_1	518	520	PF00082	0.402
CLV_PCSK_KEX2_1	879	881	PF00082	0.365
CLV_PCSK_PC1ET2_1	461	463	PF00082	0.466
CLV_PCSK_PC1ET2_1	518	520	PF00082	0.402
CLV_PCSK_SKI1_1	270	274	PF00082	0.441
CLV_PCSK_SKI1_1	449	453	PF00082	0.495
CLV_PCSK_SKI1_1	515	519	PF00082	0.404
CLV_PCSK_SKI1_1	612	616	PF00082	0.295
CLV_PCSK_SKI1_1	647	651	PF00082	0.283
CLV_PCSK_SKI1_1	751	755	PF00082	0.492
CLV_PCSK_SKI1_1	81	85	PF00082	0.339
CLV_PCSK_SKI1_1	875	879	PF00082	0.375
DEG_APCC_DBOX_1	448	456	PF00400	0.490
DEG_APCC_DBOX_1	586	594	PF00400	0.466
DEG_COP1_1	124	133	PF00400	0.583
DEG_Nend_UBRbox_1	1	4	PF02207	0.560
DEG_SCF_FBW7_2	311	317	PF00400	0.598
DOC_CKS1_1	311	316	PF01111	0.713
DOC_CYCLIN_RxL_1	748	756	PF00134	0.335
DOC_MAPK_gen_1	647	655	PF00069	0.469
DOC_MAPK_gen_1	770	778	PF00069	0.380
DOC_MAPK_gen_1	879	885	PF00069	0.374
DOC_MAPK_HePTP_8	85	97	PF00069	0.356
DOC_MAPK_MEF2A_6	128	135	PF00069	0.490
DOC_MAPK_MEF2A_6	647	655	PF00069	0.493
DOC_MAPK_MEF2A_6	770	778	PF00069	0.380
DOC_MAPK_MEF2A_6	834	842	PF00069	0.405
DOC_MAPK_MEF2A_6	88	97	PF00069	0.361
DOC_MAPK_RevD_3	604	620	PF00069	0.489
DOC_PP1_RVXF_1	447	454	PF00149	0.524
DOC_PP2B_LxvP_1	398	401	PF13499	0.686
DOC_PP4_FxxP_1	742	745	PF00568	0.544
DOC_USP7_MATH_1	103	107	PF00917	0.489
DOC_USP7_MATH_1	212	216	PF00917	0.684
DOC_USP7_MATH_1	288	292	PF00917	0.622
DOC_USP7_MATH_1	296	300	PF00917	0.719
DOC_USP7_MATH_1	305	309	PF00917	0.750
DOC_USP7_MATH_1	332	336	PF00917	0.782
DOC_USP7_MATH_1	405	409	PF00917	0.801
DOC_USP7_MATH_1	457	461	PF00917	0.392
DOC_USP7_MATH_1	651	655	PF00917	0.495
DOC_USP7_MATH_1	794	798	PF00917	0.487
DOC_USP7_MATH_1	807	811	PF00917	0.384
DOC_USP7_MATH_1	813	817	PF00917	0.408
DOC_USP7_MATH_1	922	926	PF00917	0.395
DOC_USP7_MATH_2	159	165	PF00917	0.477
DOC_WW_Pin1_4	310	315	PF00397	0.733
DOC_WW_Pin1_4	365	370	PF00397	0.669
DOC_WW_Pin1_4	38	43	PF00397	0.256
DOC_WW_Pin1_4	574	579	PF00397	0.477
DOC_WW_Pin1_4	852	857	PF00397	0.641
DOC_WW_Pin1_4	894	899	PF00397	0.552
LIG_14-3-3_CanoR_1	192	197	PF00244	0.669
LIG_14-3-3_CanoR_1	2	9	PF00244	0.536
LIG_14-3-3_CanoR_1	270	276	PF00244	0.581
LIG_14-3-3_CanoR_1	587	591	PF00244	0.503
LIG_14-3-3_CanoR_1	809	818	PF00244	0.552
LIG_Actin_WH2_2	750	766	PF00022	0.409
LIG_BRCT_BRCA1_1	194	198	PF00533	0.622
LIG_Clathr_ClatBox_1	482	486	PF01394	0.342
LIG_eIF4E_1	129	135	PF01652	0.422
LIG_eIF4E_1	588	594	PF01652	0.503
LIG_EVH1_1	35	39	PF00568	0.233
LIG_FHA_1	138	144	PF00498	0.619
LIG_FHA_1	14	20	PF00498	0.291
LIG_FHA_1	242	248	PF00498	0.348
LIG_FHA_1	250	256	PF00498	0.404
LIG_FHA_1	274	280	PF00498	0.599
LIG_FHA_1	42	48	PF00498	0.283
LIG_FHA_1	426	432	PF00498	0.813
LIG_FHA_1	470	476	PF00498	0.409
LIG_FHA_1	540	546	PF00498	0.416
LIG_FHA_1	704	710	PF00498	0.658
LIG_FHA_1	719	725	PF00498	0.481
LIG_FHA_1	78	84	PF00498	0.339
LIG_FHA_1	782	788	PF00498	0.363
LIG_FHA_1	827	833	PF00498	0.454
LIG_FHA_2	137	143	PF00498	0.565
LIG_FHA_2	148	154	PF00498	0.427
LIG_FHA_2	325	331	PF00498	0.642
LIG_FHA_2	341	347	PF00498	0.669
LIG_FHA_2	695	701	PF00498	0.497
LIG_FHA_2	868	874	PF00498	0.460
LIG_LIR_Apic_2	481	487	PF02991	0.383
LIG_LIR_Gen_1	242	253	PF02991	0.434
LIG_LIR_Gen_1	266	275	PF02991	0.485
LIG_LIR_Gen_1	62	71	PF02991	0.264
LIG_LIR_Gen_1	780	791	PF02991	0.367
LIG_LIR_Nem_3	242	248	PF02991	0.403
LIG_LIR_Nem_3	266	271	PF02991	0.472
LIG_LIR_Nem_3	486	492	PF02991	0.383
LIG_LIR_Nem_3	546	551	PF02991	0.460
LIG_LIR_Nem_3	582	588	PF02991	0.434
LIG_LIR_Nem_3	62	68	PF02991	0.264
LIG_LIR_Nem_3	780	786	PF02991	0.360
LIG_LYPXL_yS_3	35	38	PF13949	0.261
LIG_NRBOX	601	607	PF00104	0.583
LIG_NRBOX	860	866	PF00104	0.389
LIG_Pex14_1	484	488	PF04695	0.365
LIG_PTB_Apo_2	670	677	PF02174	0.495
LIG_PTB_Apo_2	752	759	PF02174	0.359
LIG_PTB_Apo_2	797	804	PF02174	0.486
LIG_PTB_Phospho_1	752	758	PF10480	0.417
LIG_PTB_Phospho_1	797	803	PF10480	0.479
LIG_SH2_CRK	243	247	PF00017	0.368
LIG_SH2_CRK	521	525	PF00017	0.351
LIG_SH2_CRK	548	552	PF00017	0.439
LIG_SH2_CRK	750	754	PF00017	0.386
LIG_SH2_CRK	783	787	PF00017	0.357
LIG_SH2_GRB2like	560	563	PF00017	0.388
LIG_SH2_GRB2like	790	793	PF00017	0.441
LIG_SH2_NCK_1	250	254	PF00017	0.542
LIG_SH2_NCK_1	521	525	PF00017	0.351
LIG_SH2_NCK_1	561	565	PF00017	0.527
LIG_SH2_PTP2	652	655	PF00017	0.495
LIG_SH2_SRC	790	793	PF00017	0.441
LIG_SH2_STAP1	194	198	PF00017	0.649
LIG_SH2_STAP1	243	247	PF00017	0.383
LIG_SH2_STAP1	250	254	PF00017	0.509
LIG_SH2_STAP1	783	787	PF00017	0.357
LIG_SH2_STAP1	803	807	PF00017	0.278
LIG_SH2_STAT3	660	663	PF00017	0.438
LIG_SH2_STAT5	129	132	PF00017	0.436
LIG_SH2_STAT5	243	246	PF00017	0.541
LIG_SH2_STAT5	250	253	PF00017	0.539
LIG_SH2_STAT5	261	264	PF00017	0.306
LIG_SH2_STAT5	498	501	PF00017	0.381
LIG_SH2_STAT5	548	551	PF00017	0.419
LIG_SH2_STAT5	558	561	PF00017	0.399
LIG_SH2_STAT5	64	67	PF00017	0.261
LIG_SH2_STAT5	652	655	PF00017	0.495
LIG_SH2_STAT5	660	663	PF00017	0.495
LIG_SH2_STAT5	667	670	PF00017	0.495
LIG_SH2_STAT5	783	786	PF00017	0.357
LIG_SH2_STAT5	790	793	PF00017	0.414
LIG_SH2_STAT5	94	97	PF00017	0.518
LIG_SH3_3	164	170	PF00018	0.569
LIG_SH3_3	33	39	PF00018	0.283
LIG_SH3_3	347	353	PF00018	0.627
LIG_SH3_3	366	372	PF00018	0.525
LIG_SH3_3	474	480	PF00018	0.363
LIG_SH3_3	484	490	PF00018	0.396
LIG_SH3_3	572	578	PF00018	0.453
LIG_SH3_3	6	12	PF00018	0.462
LIG_SH3_3	65	71	PF00018	0.268
LIG_SH3_3	793	799	PF00018	0.556
LIG_SH3_3	83	89	PF00018	0.464
LIG_SUMO_SIM_anti_2	132	137	PF11976	0.550
LIG_SUMO_SIM_anti_2	474	479	PF11976	0.342
LIG_SUMO_SIM_anti_2	784	789	PF11976	0.493
LIG_SUMO_SIM_par_1	613	618	PF11976	0.477
LIG_TRAF2_1	122	125	PF00917	0.468
LIG_TRAF2_1	139	142	PF00917	0.439
LIG_TRAF2_1	256	259	PF00917	0.576
LIG_UBA3_1	604	613	PF00899	0.498
LIG_UBA3_1	614	620	PF00899	0.489
LIG_UBA3_1	686	693	PF00899	0.585
LIG_WRC_WIRS_1	803	808	PF05994	0.564
LIG_WW_1	91	94	PF00397	0.496
MOD_CDK_SPxxK_3	368	375	PF00069	0.662
MOD_CDK_SPxxK_3	852	859	PF00069	0.436
MOD_CK1_1	106	112	PF00069	0.607
MOD_CK1_1	197	203	PF00069	0.648
MOD_CK1_1	310	316	PF00069	0.643
MOD_CK1_1	320	326	PF00069	0.718
MOD_CK1_1	368	374	PF00069	0.660
MOD_CK1_1	387	393	PF00069	0.798
MOD_CK1_1	4	10	PF00069	0.515
MOD_CK1_1	41	47	PF00069	0.263
MOD_CK1_1	49	55	PF00069	0.269
MOD_CK1_1	577	583	PF00069	0.412
MOD_CK1_1	589	595	PF00069	0.513
MOD_CK1_1	654	660	PF00069	0.495
MOD_CK1_1	694	700	PF00069	0.585
MOD_CK1_1	718	724	PF00069	0.694
MOD_CK1_1	936	942	PF00069	0.451
MOD_CK2_1	136	142	PF00069	0.563
MOD_CK2_1	147	153	PF00069	0.418
MOD_CK2_1	324	330	PF00069	0.632
MOD_CK2_1	387	393	PF00069	0.759
MOD_CK2_1	45	51	PF00069	0.282
MOD_CK2_1	589	595	PF00069	0.585
MOD_CK2_1	694	700	PF00069	0.497
MOD_GlcNHglycan	105	108	PF01048	0.666
MOD_GlcNHglycan	199	202	PF01048	0.685
MOD_GlcNHglycan	214	217	PF01048	0.536
MOD_GlcNHglycan	290	293	PF01048	0.685
MOD_GlcNHglycan	298	301	PF01048	0.822
MOD_GlcNHglycan	302	305	PF01048	0.635
MOD_GlcNHglycan	334	337	PF01048	0.725
MOD_GlcNHglycan	363	366	PF01048	0.704
MOD_GlcNHglycan	393	397	PF01048	0.824
MOD_GlcNHglycan	4	7	PF01048	0.513
MOD_GlcNHglycan	407	410	PF01048	0.609
MOD_GlcNHglycan	506	510	PF01048	0.554
MOD_GlcNHglycan	51	54	PF01048	0.353
MOD_GlcNHglycan	552	555	PF01048	0.495
MOD_GlcNHglycan	711	714	PF01048	0.644
MOD_GlcNHglycan	717	720	PF01048	0.639
MOD_GlcNHglycan	811	814	PF01048	0.468
MOD_GlcNHglycan	898	901	PF01048	0.543
MOD_GlcNHglycan	9	12	PF01048	0.401
MOD_GlcNHglycan	935	938	PF01048	0.401
MOD_GSK3_1	105	112	PF00069	0.570
MOD_GSK3_1	137	144	PF00069	0.451
MOD_GSK3_1	186	193	PF00069	0.564
MOD_GSK3_1	197	204	PF00069	0.617
MOD_GSK3_1	296	303	PF00069	0.598
MOD_GSK3_1	313	320	PF00069	0.770
MOD_GSK3_1	332	339	PF00069	0.567
MOD_GSK3_1	361	368	PF00069	0.751
MOD_GSK3_1	37	44	PF00069	0.268
MOD_GSK3_1	382	389	PF00069	0.822
MOD_GSK3_1	400	407	PF00069	0.609
MOD_GSK3_1	409	416	PF00069	0.685
MOD_GSK3_1	441	448	PF00069	0.694
MOD_GSK3_1	45	52	PF00069	0.242
MOD_GSK3_1	457	464	PF00069	0.323
MOD_GSK3_1	539	546	PF00069	0.485
MOD_GSK3_1	589	596	PF00069	0.460
MOD_GSK3_1	703	710	PF00069	0.677
MOD_GSK3_1	809	816	PF00069	0.489
MOD_N-GLC_1	136	141	PF02516	0.450
MOD_N-GLC_1	161	166	PF02516	0.466
MOD_N-GLC_1	296	301	PF02516	0.716
MOD_N-GLC_1	324	329	PF02516	0.696
MOD_N-GLC_1	336	341	PF02516	0.643
MOD_N-GLC_1	502	507	PF02516	0.578
MOD_N-GLC_1	691	696	PF02516	0.356
MOD_N-GLC_1	715	720	PF02516	0.738
MOD_N-GLC_1	77	82	PF02516	0.316
MOD_NEK2_1	1	6	PF00069	0.597
MOD_NEK2_1	13	18	PF00069	0.263
MOD_NEK2_1	190	195	PF00069	0.671
MOD_NEK2_1	263	268	PF00069	0.548
MOD_NEK2_1	271	276	PF00069	0.509
MOD_NEK2_1	324	329	PF00069	0.733
MOD_NEK2_1	340	345	PF00069	0.601
MOD_NEK2_1	392	397	PF00069	0.672
MOD_NEK2_1	566	571	PF00069	0.434
MOD_NEK2_1	593	598	PF00069	0.454
MOD_NEK2_1	615	620	PF00069	0.463
MOD_NEK2_1	709	714	PF00069	0.619
MOD_NEK2_1	753	758	PF00069	0.514
MOD_NEK2_1	781	786	PF00069	0.360
MOD_NEK2_1	802	807	PF00069	0.439
MOD_NEK2_1	818	823	PF00069	0.394
MOD_NEK2_1	867	872	PF00069	0.462
MOD_NEK2_1	893	898	PF00069	0.444
MOD_NEK2_2	336	341	PF00069	0.730
MOD_PIKK_1	111	117	PF00454	0.670
MOD_PIKK_1	382	388	PF00454	0.760
MOD_PK_1	192	198	PF00069	0.644
MOD_PKA_1	2	8	PF00069	0.523
MOD_PKA_1	281	287	PF00069	0.485
MOD_PKA_1	461	467	PF00069	0.490
MOD_PKA_1	704	710	PF00069	0.548
MOD_PKA_2	1	7	PF00069	0.547
MOD_PKA_2	103	109	PF00069	0.598
MOD_PKA_2	174	180	PF00069	0.408
MOD_PKA_2	461	467	PF00069	0.490
MOD_PKA_2	586	592	PF00069	0.466
MOD_PKA_2	703	709	PF00069	0.654
MOD_Plk_1	141	147	PF00069	0.491
MOD_Plk_1	161	167	PF00069	0.374
MOD_Plk_1	186	192	PF00069	0.541
MOD_Plk_1	241	247	PF00069	0.366
MOD_Plk_1	336	342	PF00069	0.729
MOD_Plk_1	505	511	PF00069	0.484
MOD_Plk_1	691	697	PF00069	0.586
MOD_Plk_2-3	203	209	PF00069	0.585
MOD_Plk_4	14	20	PF00069	0.309
MOD_Plk_4	241	247	PF00069	0.391
MOD_Plk_4	274	280	PF00069	0.486
MOD_Plk_4	307	313	PF00069	0.715
MOD_Plk_4	478	484	PF00069	0.380
MOD_Plk_4	589	595	PF00069	0.502
MOD_Plk_4	691	697	PF00069	0.619
MOD_Plk_4	753	759	PF00069	0.520
MOD_Plk_4	781	787	PF00069	0.362
MOD_Plk_4	819	825	PF00069	0.392
MOD_ProDKin_1	310	316	PF00069	0.733
MOD_ProDKin_1	365	371	PF00069	0.670
MOD_ProDKin_1	38	44	PF00069	0.256
MOD_ProDKin_1	574	580	PF00069	0.477
MOD_ProDKin_1	852	858	PF00069	0.634
MOD_ProDKin_1	894	900	PF00069	0.557
MOD_SUMO_for_1	184	187	PF00179	0.564
MOD_SUMO_rev_2	685	695	PF00179	0.585
MOD_SUMO_rev_2	914	922	PF00179	0.403
TRG_DiLeu_BaEn_1	611	616	PF01217	0.495
TRG_DiLeu_BaEn_1	836	841	PF01217	0.378
TRG_DiLeu_BaLyEn_6	267	272	PF01217	0.493
TRG_DiLeu_BaLyEn_6	9	14	PF01217	0.361
TRG_ENDOCYTIC_2	243	246	PF00928	0.392
TRG_ENDOCYTIC_2	35	38	PF00928	0.261
TRG_ENDOCYTIC_2	521	524	PF00928	0.374
TRG_ENDOCYTIC_2	548	551	PF00928	0.419
TRG_ENDOCYTIC_2	652	655	PF00928	0.495
TRG_ENDOCYTIC_2	750	753	PF00928	0.401
TRG_ENDOCYTIC_2	783	786	PF00928	0.357
TRG_ENDOCYTIC_2	803	806	PF00928	0.277
TRG_ENDOCYTIC_2	94	97	PF00928	0.423
TRG_ER_diArg_1	120	122	PF00400	0.569
TRG_ER_diArg_1	773	776	PF00400	0.370
TRG_ER_diArg_1	878	880	PF00400	0.364

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P522	Leptomonas seymouri	58%	94%
A0A3S7X3N9	Leishmania donovani	100%	100%
A4HIK5	Leishmania braziliensis	74%	100%
E9B145	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
Q4Q721	Leishmania major	92%	100%