ATP-dependent DNA helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA repair and recombination helicase protein PIF3 - putative

Species:

Leishmania infantum

UniProt:

A4I3Y5_LEIIN

TriTrypDb:

LINF_280032500

Length:

937

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0005657	replication fork	2	1

Expansion

Sequence features

A4I3Y5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I3Y5

Function

Biological processes
Term	Name	Level	Count
GO:0000723	telomere maintenance	5	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006281	DNA repair	5	11
GO:0006310	DNA recombination	5	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0006996	organelle organization	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016043	cellular component organization	3	11
GO:0032200	telomere organization	6	11
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0050896	response to stimulus	1	11
GO:0051276	chromosome organization	5	11
GO:0051716	cellular response to stimulus	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0071840	cellular component organization or biogenesis	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:0006260	DNA replication	5	1
GO:0032392	DNA geometric change	7	1
GO:0032508	DNA duplex unwinding	8	1
GO:0071103	DNA conformation change	6	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003678	DNA helicase activity	3	11
GO:0003824	catalytic activity	1	11
GO:0004386	helicase activity	2	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0008094	ATP-dependent activity, acting on DNA	2	11
GO:0016462	pyrophosphatase activity	5	11
GO:0016787	hydrolase activity	2	11
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	11
GO:0016887	ATP hydrolysis activity	7	11
GO:0017076	purine nucleotide binding	4	11
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140097	catalytic activity, acting on DNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:0140657	ATP-dependent activity	1	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0000287	magnesium ion binding	5	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	379	383	PF00656	0.335
CLV_C14_Caspase3-7	619	623	PF00656	0.791
CLV_NRD_NRD_1	157	159	PF00675	0.642
CLV_NRD_NRD_1	227	229	PF00675	0.505
CLV_NRD_NRD_1	459	461	PF00675	0.460
CLV_NRD_NRD_1	489	491	PF00675	0.457
CLV_NRD_NRD_1	564	566	PF00675	0.737
CLV_NRD_NRD_1	661	663	PF00675	0.700
CLV_NRD_NRD_1	674	676	PF00675	0.627
CLV_NRD_NRD_1	774	776	PF00675	0.507
CLV_NRD_NRD_1	875	877	PF00675	0.527
CLV_NRD_NRD_1	894	896	PF00675	0.374
CLV_PCSK_FUR_1	672	676	PF00082	0.726
CLV_PCSK_KEX2_1	156	158	PF00082	0.667
CLV_PCSK_KEX2_1	227	229	PF00082	0.548
CLV_PCSK_KEX2_1	459	461	PF00082	0.521
CLV_PCSK_KEX2_1	488	490	PF00082	0.480
CLV_PCSK_KEX2_1	671	673	PF00082	0.715
CLV_PCSK_KEX2_1	674	676	PF00082	0.673
CLV_PCSK_KEX2_1	774	776	PF00082	0.507
CLV_PCSK_KEX2_1	894	896	PF00082	0.502
CLV_PCSK_PC1ET2_1	671	673	PF00082	0.751
CLV_PCSK_PC7_1	152	158	PF00082	0.602
CLV_PCSK_PC7_1	223	229	PF00082	0.566
CLV_PCSK_SKI1_1	115	119	PF00082	0.422
CLV_PCSK_SKI1_1	167	171	PF00082	0.640
CLV_PCSK_SKI1_1	223	227	PF00082	0.594
CLV_PCSK_SKI1_1	292	296	PF00082	0.342
CLV_PCSK_SKI1_1	310	314	PF00082	0.311
CLV_PCSK_SKI1_1	438	442	PF00082	0.354
CLV_PCSK_SKI1_1	596	600	PF00082	0.476
CLV_PCSK_SKI1_1	674	678	PF00082	0.649
CLV_PCSK_SKI1_1	699	703	PF00082	0.518
CLV_PCSK_SKI1_1	781	785	PF00082	0.575
CLV_PCSK_SKI1_1	895	899	PF00082	0.490
DEG_APCC_DBOX_1	544	552	PF00400	0.522
DEG_Nend_UBRbox_2	1	3	PF02207	0.675
DEG_SPOP_SBC_1	107	111	PF00917	0.533
DEG_SPOP_SBC_1	66	70	PF00917	0.685
DOC_CKS1_1	270	275	PF01111	0.617
DOC_CKS1_1	439	444	PF01111	0.408
DOC_CKS1_1	509	514	PF01111	0.501
DOC_CYCLIN_yCln2_LP_2	270	276	PF00134	0.615
DOC_CYCLIN_yCln2_LP_2	439	445	PF00134	0.408
DOC_CYCLIN_yCln2_LP_2	471	477	PF00134	0.614
DOC_CYCLIN_yCln2_LP_2	830	836	PF00134	0.517
DOC_MAPK_DCC_7	791	801	PF00069	0.513
DOC_MAPK_gen_1	662	668	PF00069	0.639
DOC_MAPK_gen_1	736	745	PF00069	0.389
DOC_MAPK_gen_1	774	784	PF00069	0.564
DOC_MAPK_gen_1	874	883	PF00069	0.395
DOC_MAPK_gen_1	894	900	PF00069	0.232
DOC_MAPK_MEF2A_6	319	328	PF00069	0.285
DOC_MAPK_MEF2A_6	401	410	PF00069	0.314
DOC_MAPK_MEF2A_6	432	439	PF00069	0.336
DOC_MAPK_RevD_3	881	895	PF00069	0.454
DOC_PP1_RVXF_1	779	785	PF00149	0.544
DOC_PP2B_LxvP_1	830	833	PF13499	0.519
DOC_PP4_FxxP_1	21	24	PF00568	0.642
DOC_PP4_FxxP_1	350	353	PF00568	0.335
DOC_PP4_FxxP_1	764	767	PF00568	0.420
DOC_PP4_FxxP_1	98	101	PF00568	0.397
DOC_USP7_MATH_1	189	193	PF00917	0.791
DOC_USP7_MATH_1	194	198	PF00917	0.756
DOC_USP7_MATH_1	425	429	PF00917	0.406
DOC_USP7_MATH_1	573	577	PF00917	0.660
DOC_USP7_MATH_1	65	69	PF00917	0.734
DOC_USP7_MATH_1	767	771	PF00917	0.557
DOC_USP7_MATH_1	907	911	PF00917	0.621
DOC_USP7_MATH_1	919	923	PF00917	0.538
DOC_WW_Pin1_4	132	137	PF00397	0.535
DOC_WW_Pin1_4	269	274	PF00397	0.549
DOC_WW_Pin1_4	281	286	PF00397	0.268
DOC_WW_Pin1_4	438	443	PF00397	0.408
DOC_WW_Pin1_4	508	513	PF00397	0.475
DOC_WW_Pin1_4	57	62	PF00397	0.735
DOC_WW_Pin1_4	730	735	PF00397	0.453
DOC_WW_Pin1_4	74	79	PF00397	0.695
DOC_WW_Pin1_4	912	917	PF00397	0.584
LIG_14-3-3_CanoR_1	108	118	PF00244	0.505
LIG_14-3-3_CanoR_1	193	202	PF00244	0.760
LIG_14-3-3_CanoR_1	327	336	PF00244	0.368
LIG_14-3-3_CanoR_1	44	48	PF00244	0.721
LIG_14-3-3_CanoR_1	631	636	PF00244	0.600
LIG_14-3-3_CanoR_1	647	656	PF00244	0.632
LIG_14-3-3_CanoR_1	674	679	PF00244	0.616
LIG_14-3-3_CanoR_1	699	704	PF00244	0.501
LIG_14-3-3_CanoR_1	774	782	PF00244	0.575
LIG_14-3-3_CanoR_1	894	900	PF00244	0.530
LIG_14-3-3_CanoR_1	921	925	PF00244	0.452
LIG_14-3-3_CanoR_1	926	933	PF00244	0.544
LIG_Actin_WH2_2	247	264	PF00022	0.524
LIG_Actin_WH2_2	314	329	PF00022	0.335
LIG_Actin_WH2_2	544	562	PF00022	0.424
LIG_Actin_WH2_2	725	740	PF00022	0.548
LIG_BIR_III_4	534	538	PF00653	0.647
LIG_BRCT_BRCA1_1	427	431	PF00533	0.335
LIG_BRCT_BRCA1_1	90	94	PF00533	0.573
LIG_EH_1	99	103	PF12763	0.420
LIG_FHA_1	232	238	PF00498	0.549
LIG_FHA_1	309	315	PF00498	0.335
LIG_FHA_1	331	337	PF00498	0.335
LIG_FHA_1	477	483	PF00498	0.584
LIG_FHA_1	593	599	PF00498	0.516
LIG_FHA_1	715	721	PF00498	0.610
LIG_FHA_1	829	835	PF00498	0.519
LIG_FHA_1	84	90	PF00498	0.591
LIG_FHA_1	851	857	PF00498	0.380
LIG_FHA_2	270	276	PF00498	0.530
LIG_FHA_2	681	687	PF00498	0.454
LIG_FHA_2	700	706	PF00498	0.506
LIG_FHA_2	717	723	PF00498	0.503
LIG_Integrin_isoDGR_2	103	105	PF01839	0.593
LIG_LIR_Apic_2	483	487	PF02991	0.426
LIG_LIR_Apic_2	762	767	PF02991	0.424
LIG_LIR_Apic_2	96	101	PF02991	0.409
LIG_LIR_Gen_1	245	254	PF02991	0.545
LIG_LIR_Gen_1	413	421	PF02991	0.335
LIG_LIR_Gen_1	466	476	PF02991	0.557
LIG_LIR_LC3C_4	879	883	PF02991	0.497
LIG_LIR_Nem_3	245	250	PF02991	0.503
LIG_LIR_Nem_3	33	37	PF02991	0.769
LIG_LIR_Nem_3	413	417	PF02991	0.328
LIG_LIR_Nem_3	428	434	PF02991	0.335
LIG_LIR_Nem_3	466	471	PF02991	0.553
LIG_LIR_Nem_3	683	688	PF02991	0.444
LIG_LIR_Nem_3	88	93	PF02991	0.645
LIG_PCNA_yPIPBox_3	251	262	PF02747	0.559
LIG_PCNA_yPIPBox_3	791	802	PF02747	0.546
LIG_PDZ_Class_2	932	937	PF00595	0.656
LIG_Pex14_2	118	122	PF04695	0.513
LIG_Pex14_2	346	350	PF04695	0.321
LIG_Pex14_2	94	98	PF04695	0.450
LIG_SH2_CRK	123	127	PF00017	0.553
LIG_SH2_CRK	258	262	PF00017	0.567
LIG_SH2_CRK	265	269	PF00017	0.584
LIG_SH2_CRK	90	94	PF00017	0.606
LIG_SH2_GRB2like	249	252	PF00017	0.557
LIG_SH2_NCK_1	426	430	PF00017	0.440
LIG_SH2_STAP1	426	430	PF00017	0.447
LIG_SH2_STAP1	838	842	PF00017	0.477
LIG_SH2_STAT3	884	887	PF00017	0.570
LIG_SH2_STAT5	249	252	PF00017	0.493
LIG_SH2_STAT5	484	487	PF00017	0.427
LIG_SH2_STAT5	823	826	PF00017	0.478
LIG_SH2_STAT5	869	872	PF00017	0.366
LIG_SH3_3	123	129	PF00018	0.491
LIG_SH3_3	16	22	PF00018	0.733
LIG_SH3_3	25	31	PF00018	0.718
LIG_SH3_3	270	276	PF00018	0.657
LIG_SH3_3	471	477	PF00018	0.552
LIG_SH3_3	580	586	PF00018	0.455
LIG_SH3_3	804	810	PF00018	0.411
LIG_SH3_CIN85_PxpxPR_1	22	27	PF14604	0.702
LIG_SUMO_SIM_anti_2	376	382	PF11976	0.340
LIG_SUMO_SIM_anti_2	581	586	PF11976	0.557
LIG_SUMO_SIM_par_1	664	670	PF11976	0.652
LIG_SUMO_SIM_par_1	712	717	PF11976	0.471
LIG_SUMO_SIM_par_1	852	857	PF11976	0.374
MOD_CDK_SPxK_1	438	444	PF00069	0.408
MOD_CDK_SPxK_1	508	514	PF00069	0.529
MOD_CDK_SPxK_1	730	736	PF00069	0.497
MOD_CK1_1	179	185	PF00069	0.698
MOD_CK1_1	305	311	PF00069	0.378
MOD_CK1_1	629	635	PF00069	0.751
MOD_CK1_1	667	673	PF00069	0.630
MOD_CK1_1	70	76	PF00069	0.766
MOD_CK1_1	754	760	PF00069	0.414
MOD_CK1_1	922	928	PF00069	0.513
MOD_CK2_1	132	138	PF00069	0.541
MOD_CK2_1	678	684	PF00069	0.550
MOD_CK2_1	699	705	PF00069	0.542
MOD_CK2_1	893	899	PF00069	0.425
MOD_GlcNHglycan	111	114	PF01048	0.424
MOD_GlcNHglycan	14	17	PF01048	0.706
MOD_GlcNHglycan	179	182	PF01048	0.682
MOD_GlcNHglycan	196	199	PF01048	0.764
MOD_GlcNHglycan	304	307	PF01048	0.371
MOD_GlcNHglycan	427	430	PF01048	0.319
MOD_GlcNHglycan	525	528	PF01048	0.530
MOD_GlcNHglycan	622	625	PF01048	0.784
MOD_GlcNHglycan	680	683	PF01048	0.579
MOD_GlcNHglycan	69	72	PF01048	0.777
MOD_GlcNHglycan	895	898	PF01048	0.415
MOD_GlcNHglycan	90	93	PF01048	0.536
MOD_GSK3_1	177	184	PF00069	0.745
MOD_GSK3_1	189	196	PF00069	0.668
MOD_GSK3_1	275	282	PF00069	0.584
MOD_GSK3_1	39	46	PF00069	0.726
MOD_GSK3_1	415	422	PF00069	0.382
MOD_GSK3_1	476	483	PF00069	0.562
MOD_GSK3_1	519	526	PF00069	0.599
MOD_GSK3_1	626	633	PF00069	0.743
MOD_GSK3_1	645	652	PF00069	0.527
MOD_GSK3_1	66	73	PF00069	0.731
MOD_GSK3_1	670	677	PF00069	0.684
MOD_GSK3_1	687	694	PF00069	0.491
MOD_GSK3_1	712	719	PF00069	0.586
MOD_GSK3_1	74	81	PF00069	0.662
MOD_GSK3_1	747	754	PF00069	0.443
MOD_GSK3_1	846	853	PF00069	0.399
MOD_N-GLC_1	477	482	PF02516	0.546
MOD_N-GLC_1	747	752	PF02516	0.468
MOD_N-GLC_1	754	759	PF02516	0.414
MOD_NEK2_1	106	111	PF00069	0.486
MOD_NEK2_1	279	284	PF00069	0.584
MOD_NEK2_1	330	335	PF00069	0.335
MOD_NEK2_1	354	359	PF00069	0.273
MOD_NEK2_1	43	48	PF00069	0.711
MOD_NEK2_1	519	524	PF00069	0.504
MOD_NEK2_1	714	719	PF00069	0.540
MOD_NEK2_1	749	754	PF00069	0.442
MOD_NEK2_1	816	821	PF00069	0.584
MOD_NEK2_1	850	855	PF00069	0.381
MOD_NEK2_1	94	99	PF00069	0.541
MOD_NEK2_2	342	347	PF00069	0.354
MOD_NEK2_2	657	662	PF00069	0.650
MOD_NEK2_2	767	772	PF00069	0.497
MOD_NEK2_2	838	843	PF00069	0.381
MOD_OFUCOSY	48	54	PF10250	0.561
MOD_PIKK_1	146	152	PF00454	0.589
MOD_PIKK_1	216	222	PF00454	0.594
MOD_PIKK_1	502	508	PF00454	0.561
MOD_PKA_1	674	680	PF00069	0.606
MOD_PKA_2	107	113	PF00069	0.469
MOD_PKA_2	176	182	PF00069	0.657
MOD_PKA_2	194	200	PF00069	0.737
MOD_PKA_2	43	49	PF00069	0.721
MOD_PKA_2	630	636	PF00069	0.766
MOD_PKA_2	674	680	PF00069	0.642
MOD_PKA_2	773	779	PF00069	0.631
MOD_PKA_2	893	899	PF00069	0.493
MOD_PKA_2	907	913	PF00069	0.490
MOD_PKA_2	920	926	PF00069	0.406
MOD_PKA_2	94	100	PF00069	0.532
MOD_PKB_1	672	680	PF00069	0.624
MOD_Plk_1	376	382	PF00069	0.335
MOD_Plk_1	419	425	PF00069	0.381
MOD_Plk_1	477	483	PF00069	0.500
MOD_Plk_1	767	773	PF00069	0.543
MOD_Plk_2-3	376	382	PF00069	0.335
MOD_Plk_4	308	314	PF00069	0.373
MOD_Plk_4	376	382	PF00069	0.335
MOD_Plk_4	699	705	PF00069	0.499
MOD_Plk_4	707	713	PF00069	0.455
MOD_Plk_4	767	773	PF00069	0.524
MOD_Plk_4	819	825	PF00069	0.424
MOD_Plk_4	850	856	PF00069	0.378
MOD_Plk_4	94	100	PF00069	0.504
MOD_ProDKin_1	132	138	PF00069	0.541
MOD_ProDKin_1	269	275	PF00069	0.555
MOD_ProDKin_1	281	287	PF00069	0.268
MOD_ProDKin_1	438	444	PF00069	0.408
MOD_ProDKin_1	508	514	PF00069	0.482
MOD_ProDKin_1	57	63	PF00069	0.737
MOD_ProDKin_1	730	736	PF00069	0.449
MOD_ProDKin_1	74	80	PF00069	0.692
MOD_ProDKin_1	912	918	PF00069	0.592
MOD_SUMO_rev_2	491	500	PF00179	0.432
TRG_DiLeu_BaEn_3	706	712	PF01217	0.540
TRG_DiLeu_BaEn_4	554	560	PF01217	0.526
TRG_DiLeu_BaLyEn_6	350	355	PF01217	0.273
TRG_DiLeu_BaLyEn_6	544	549	PF01217	0.637
TRG_DiLeu_BaLyEn_6	797	802	PF01217	0.440
TRG_ENDOCYTIC_2	123	126	PF00928	0.578
TRG_ENDOCYTIC_2	258	261	PF00928	0.448
TRG_ENDOCYTIC_2	318	321	PF00928	0.479
TRG_ENDOCYTIC_2	90	93	PF00928	0.580
TRG_ER_diArg_1	156	158	PF00400	0.644
TRG_ER_diArg_1	226	228	PF00400	0.551
TRG_ER_diArg_1	458	460	PF00400	0.507
TRG_ER_diArg_1	487	490	PF00400	0.466
TRG_ER_diArg_1	599	602	PF00400	0.422
TRG_ER_diArg_1	672	675	PF00400	0.705
TRG_ER_diArg_1	773	775	PF00400	0.479
TRG_ER_diArg_1	893	895	PF00400	0.595
TRG_NLS_MonoExtC_3	184	190	PF00514	0.624
TRG_NLS_MonoExtC_3	670	675	PF00514	0.733
TRG_Pf-PMV_PEXEL_1	319	323	PF00026	0.304
TRG_Pf-PMV_PEXEL_1	781	785	PF00026	0.444
TRG_Pf-PMV_PEXEL_1	800	805	PF00026	0.418
TRG_Pf-PMV_PEXEL_1	876	880	PF00026	0.470

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCJ6	Leptomonas seymouri	59%	84%
A0A1X0NSB2	Trypanosomatidae	51%	100%
A0A3Q8IGY7	Leishmania donovani	100%	100%
A0A422NKI2	Trypanosoma rangeli	50%	100%
A4HGV5	Leishmania braziliensis	80%	100%
D0A956	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	100%
E9B075	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
Q383A1	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	48%	100%
Q4Q810	Leishmania major	92%	100%
V5BL79	Trypanosoma cruzi	50%	100%