LeishMANIAdb
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Replication protein A subunit

Quick info Annotations Function or PPIs Localization Phosphorylation Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:
Replication protein A subunit
Gene product:
replication factor A - 51kDa subunit - putative
Species:
Leishmania infantum
UniProt:
A4I3Q8_LEIIN
TriTrypDb:
LINF_280024500
Length:
467

Annotations

Annotations by Jardim et al.

DNA replication, replication factor A, 51kDa subunit RPA1

Localization

Secreted promastigote
Source Evidence on protein Close homologs
Cuervo et al. no yes: 0
Hassani et al. no yes: 0
Forrest at al. (metacyclic) no yes: 0
Forrest at al. (procyclic) no yes: 0
Silverman et al. no yes: 4
Pissara et al. yes yes: 20
Secreted amastigote
Source Evidence on protein Close homologs
Pires et al. no yes: 0
Exosome
Source Evidence on protein Close homologs
Silverman et al. no yes: 0
Glycosome
Source Evidence on protein Close homologs
Jamdhade et al. no yes: 0
Predictions
Source Evidence on protein Close homologs
DeepLoc
SignalP6 no yes: 0, no: 6
NetGPI no yes: 0, no: 6
Cellular components
Term Name Level Count
GO:0005634 nucleus 5 6
GO:0043226 organelle 2 6
GO:0043227 membrane-bounded organelle 3 6
GO:0043229 intracellular organelle 3 6
GO:0043231 intracellular membrane-bounded organelle 4 6
GO:0110165 cellular anatomical entity 1 7
GO:0005662 DNA replication factor A complex 3 1
GO:0032991 protein-containing complex 1 1
GO:0034399 nuclear periphery 2 1
GO:0140513 nuclear protein-containing complex 2 1

Phosphorylation

Amastigote: 119, 120

Abundance

Amastigote (protein)
Source Evidence on protein Close homologs
Pescher et al. (upgregulation) no yes: 0
Promastigote and amastigote
Source Evidence on protein Close homologs
Lahav et al.
- mRNA
- Protein

Expansion

Sequence features

A4I3Q8
Sequence
MSA
Disorder
Secondary
Topology
Domains
SignalP
GPI
Phosphorylations
ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I3Q8

Function

Biological processes
Term Name Level Count
GO:0006139 nucleobase-containing compound metabolic process 3 7
GO:0006259 DNA metabolic process 4 7
GO:0006260 DNA replication 5 6
GO:0006281 DNA repair 5 7
GO:0006310 DNA recombination 5 7
GO:0006725 cellular aromatic compound metabolic process 3 7
GO:0006807 nitrogen compound metabolic process 2 7
GO:0006950 response to stress 2 7
GO:0006974 DNA damage response 4 7
GO:0008152 metabolic process 1 7
GO:0009987 cellular process 1 7
GO:0033554 cellular response to stress 3 7
GO:0034641 cellular nitrogen compound metabolic process 3 7
GO:0043170 macromolecule metabolic process 3 7
GO:0044237 cellular metabolic process 2 7
GO:0044238 primary metabolic process 2 7
GO:0044260 obsolete cellular macromolecule metabolic process 3 7
GO:0046483 heterocycle metabolic process 3 7
GO:0050896 response to stimulus 1 7
GO:0051716 cellular response to stimulus 2 7
GO:0071704 organic substance metabolic process 2 7
GO:0090304 nucleic acid metabolic process 4 7
GO:1901360 organic cyclic compound metabolic process 3 7
GO:0000723 telomere maintenance 5 1
GO:0000724 double-strand break repair via homologous recombination 7 1
GO:0000725 recombinational repair 6 1
GO:0006268 DNA unwinding involved in DNA replication 9 1
GO:0006278 RNA-templated DNA biosynthetic process 6 1
GO:0006289 nucleotide-excision repair 6 1
GO:0006302 double-strand break repair 6 1
GO:0006996 organelle organization 4 1
GO:0007004 telomere maintenance via telomerase 7 1
GO:0007049 cell cycle 2 1
GO:0009058 biosynthetic process 2 1
GO:0009059 macromolecule biosynthetic process 4 1
GO:0010833 telomere maintenance via telomere lengthening 6 1
GO:0016043 cellular component organization 3 1
GO:0018130 heterocycle biosynthetic process 4 1
GO:0019438 aromatic compound biosynthetic process 4 1
GO:0022414 reproductive process 1 1
GO:0032200 telomere organization 6 1
GO:0032392 DNA geometric change 7 1
GO:0032508 DNA duplex unwinding 8 1
GO:0034654 nucleobase-containing compound biosynthetic process 4 1
GO:0044249 cellular biosynthetic process 3 1
GO:0044271 cellular nitrogen compound biosynthetic process 4 1
GO:0051276 chromosome organization 5 1
GO:0051321 meiotic cell cycle 2 1
GO:0071103 DNA conformation change 6 1
GO:0071840 cellular component organization or biogenesis 2 1
GO:0071897 DNA biosynthetic process 5 1
GO:1901362 organic cyclic compound biosynthetic process 4 1
GO:1901576 organic substance biosynthetic process 3 1
Molecular functions
Term Name Level Count
GO:0003676 nucleic acid binding 3 7
GO:0003677 DNA binding 4 7
GO:0005488 binding 1 7
GO:0043167 ion binding 2 7
GO:0043169 cation binding 3 7
GO:0046872 metal ion binding 4 7
GO:0097159 organic cyclic compound binding 2 7
GO:1901363 heterocyclic compound binding 2 7
GO:0003684 damaged DNA binding 5 1
GO:0003697 single-stranded DNA binding 5 1
GO:0042162 telomeric DNA binding 6 1
GO:0043047 single-stranded telomeric DNA binding 7 1
GO:0043565 sequence-specific DNA binding 5 1
GO:0098847 sequence-specific single stranded DNA binding 6 1

Putative motif mimicry

Leishmania From To Domain/Motif Score
CLV_NRD_NRD_1 273 275 PF00675 0.477
CLV_NRD_NRD_1 380 382 PF00675 0.359
CLV_NRD_NRD_1 446 448 PF00675 0.494
CLV_NRD_NRD_1 93 95 PF00675 0.294
CLV_PCSK_KEX2_1 172 174 PF00082 0.359
CLV_PCSK_KEX2_1 379 381 PF00082 0.359
CLV_PCSK_KEX2_1 445 447 PF00082 0.557
CLV_PCSK_KEX2_1 93 95 PF00082 0.294
CLV_PCSK_PC1ET2_1 172 174 PF00082 0.359
CLV_PCSK_PC1ET2_1 379 381 PF00082 0.359
CLV_PCSK_PC7_1 168 174 PF00082 0.359
CLV_PCSK_SKI1_1 173 177 PF00082 0.359
CLV_PCSK_SKI1_1 207 211 PF00082 0.359
CLV_PCSK_SKI1_1 239 243 PF00082 0.466
CLV_PCSK_SKI1_1 287 291 PF00082 0.594
CLV_PCSK_SKI1_1 318 322 PF00082 0.359
CLV_PCSK_SKI1_1 337 341 PF00082 0.461
CLV_Separin_Metazoa 458 462 PF03568 0.578
DEG_APCC_DBOX_1 273 281 PF00400 0.469
DEG_Nend_UBRbox_3 1 3 PF02207 0.643
DOC_MAPK_gen_1 287 296 PF00069 0.449
DOC_MAPK_gen_1 396 405 PF00069 0.359
DOC_MAPK_gen_1 423 431 PF00069 0.359
DOC_MAPK_MEF2A_6 128 136 PF00069 0.436
DOC_MAPK_MEF2A_6 344 351 PF00069 0.359
DOC_MAPK_MEF2A_6 399 407 PF00069 0.359
DOC_USP7_MATH_1 11 15 PF00917 0.452
DOC_USP7_MATH_1 438 442 PF00917 0.567
DOC_USP7_UBL2_3 172 176 PF12436 0.359
DOC_WW_Pin1_4 13 18 PF00397 0.439
DOC_WW_Pin1_4 337 342 PF00397 0.461
LIG_14-3-3_CanoR_1 108 114 PF00244 0.458
LIG_14-3-3_CanoR_1 26 35 PF00244 0.567
LIG_14-3-3_CanoR_1 423 429 PF00244 0.359
LIG_14-3-3_CanoR_1 430 437 PF00244 0.514
LIG_14-3-3_CanoR_1 60 64 PF00244 0.494
LIG_Actin_WH2_2 196 212 PF00022 0.359
LIG_APCC_ABBA_1 360 365 PF00400 0.359
LIG_APCC_ABBAyCdc20_2 60 66 PF00400 0.494
LIG_BIR_III_2 311 315 PF00653 0.359
LIG_FHA_1 119 125 PF00498 0.493
LIG_FHA_1 14 20 PF00498 0.440
LIG_FHA_1 179 185 PF00498 0.359
LIG_FHA_1 240 246 PF00498 0.454
LIG_FHA_1 350 356 PF00498 0.351
LIG_FHA_2 164 170 PF00498 0.359
LIG_FHA_2 223 229 PF00498 0.317
LIG_FHA_2 415 421 PF00498 0.359
LIG_FHA_2 438 444 PF00498 0.560
LIG_FHA_2 452 458 PF00498 0.399
LIG_FHA_2 49 55 PF00498 0.494
LIG_GBD_Chelix_1 145 153 PF00786 0.359
LIG_LIR_Apic_2 37 41 PF02991 0.494
LIG_LIR_Gen_1 292 301 PF02991 0.323
LIG_LIR_Gen_1 356 366 PF02991 0.359
LIG_LIR_Gen_1 49 58 PF02991 0.494
LIG_LIR_Nem_3 213 217 PF02991 0.359
LIG_LIR_Nem_3 292 296 PF02991 0.323
LIG_LIR_Nem_3 356 362 PF02991 0.359
LIG_LIR_Nem_3 432 437 PF02991 0.536
LIG_LIR_Nem_3 49 53 PF02991 0.476
LIG_LIR_Nem_3 69 74 PF02991 0.331
LIG_PCNA_yPIPBox_3 138 146 PF02747 0.359
LIG_Pex14_1 359 363 PF04695 0.359
LIG_SH2_CRK 129 133 PF00017 0.433
LIG_SH2_PTP2 293 296 PF00017 0.359
LIG_SH2_SRC 293 296 PF00017 0.359
LIG_SH2_STAP1 102 106 PF00017 0.494
LIG_SH2_STAT3 302 305 PF00017 0.359
LIG_SH2_STAT5 244 247 PF00017 0.467
LIG_SH2_STAT5 276 279 PF00017 0.410
LIG_SH2_STAT5 293 296 PF00017 0.355
LIG_SH2_STAT5 302 305 PF00017 0.359
LIG_SH2_STAT5 345 348 PF00017 0.300
LIG_SH2_STAT5 388 391 PF00017 0.359
LIG_SH2_STAT5 82 85 PF00017 0.494
LIG_SH3_4 40 47 PF00018 0.494
LIG_SUMO_SIM_anti_2 144 150 PF11976 0.359
LIG_TRAF2_1 417 420 PF00917 0.323
LIG_TRAF2_1 440 443 PF00917 0.527
LIG_WRC_WIRS_1 211 216 PF05994 0.359
LIG_WRC_WIRS_1 47 52 PF05994 0.494
LIG_WRC_WIRS_1 68 73 PF05994 0.474
MOD_CDK_SPxxK_3 337 344 PF00069 0.461
MOD_CK1_1 159 165 PF00069 0.359
MOD_CK1_1 254 260 PF00069 0.479
MOD_CK1_1 441 447 PF00069 0.550
MOD_CK2_1 163 169 PF00069 0.359
MOD_CK2_1 414 420 PF00069 0.323
MOD_CK2_1 437 443 PF00069 0.548
MOD_Cter_Amidation 272 275 PF01082 0.485
MOD_GlcNHglycan 263 266 PF01048 0.577
MOD_GlcNHglycan 54 58 PF01048 0.294
MOD_GlcNHglycan 83 86 PF01048 0.294
MOD_GSK3_1 102 109 PF00069 0.552
MOD_GSK3_1 159 166 PF00069 0.359
MOD_GSK3_1 215 222 PF00069 0.359
MOD_GSK3_1 239 246 PF00069 0.414
MOD_GSK3_1 250 257 PF00069 0.417
MOD_GSK3_1 30 37 PF00069 0.494
MOD_GSK3_1 335 342 PF00069 0.303
MOD_GSK3_1 345 352 PF00069 0.294
MOD_GSK3_1 410 417 PF00069 0.359
MOD_GSK3_1 437 444 PF00069 0.564
MOD_N-GLC_1 250 255 PF02516 0.544
MOD_N-GLC_1 353 358 PF02516 0.359
MOD_NEK2_1 219 224 PF00069 0.355
MOD_NEK2_1 424 429 PF00069 0.359
MOD_NEK2_1 48 53 PF00069 0.452
MOD_NEK2_2 358 363 PF00069 0.461
MOD_PIKK_1 159 165 PF00454 0.335
MOD_PIKK_1 254 260 PF00454 0.531
MOD_PIKK_1 339 345 PF00454 0.359
MOD_PIKK_1 353 359 PF00454 0.359
MOD_PIKK_1 40 46 PF00454 0.494
MOD_PKA_1 318 324 PF00069 0.359
MOD_PKA_1 93 99 PF00069 0.494
MOD_PKA_2 107 113 PF00069 0.457
MOD_PKA_2 27 33 PF00069 0.567
MOD_PKA_2 424 430 PF00069 0.355
MOD_PKA_2 59 65 PF00069 0.494
MOD_PKA_2 93 99 PF00069 0.494
MOD_Plk_1 102 108 PF00069 0.534
MOD_Plk_1 156 162 PF00069 0.332
MOD_Plk_1 184 190 PF00069 0.359
MOD_Plk_1 250 256 PF00069 0.538
MOD_Plk_1 335 341 PF00069 0.348
MOD_Plk_2-3 141 147 PF00069 0.359
MOD_Plk_4 141 147 PF00069 0.359
MOD_Plk_4 184 190 PF00069 0.359
MOD_Plk_4 199 205 PF00069 0.359
MOD_Plk_4 239 245 PF00069 0.452
MOD_Plk_4 358 364 PF00069 0.315
MOD_Plk_4 424 430 PF00069 0.359
MOD_Plk_4 59 65 PF00069 0.494
MOD_ProDKin_1 13 19 PF00069 0.441
MOD_ProDKin_1 337 343 PF00069 0.461
MOD_SUMO_for_1 289 292 PF00179 0.474
MOD_SUMO_for_1 407 410 PF00179 0.359
MOD_SUMO_rev_2 191 195 PF00179 0.337
MOD_SUMO_rev_2 233 240 PF00179 0.467
TRG_DiLeu_BaEn_1 141 146 PF01217 0.359
TRG_DiLeu_BaEn_1 458 463 PF01217 0.577
TRG_DiLeu_BaEn_4 419 425 PF01217 0.359
TRG_DiLeu_BaLyEn_6 70 75 PF01217 0.567
TRG_DiLeu_LyEn_5 458 463 PF01217 0.577
TRG_ENDOCYTIC_2 129 132 PF00928 0.430
TRG_ENDOCYTIC_2 293 296 PF00928 0.344
TRG_ER_diArg_1 25 28 PF00400 0.488
TRG_ER_diArg_1 403 406 PF00400 0.303
TRG_ER_diArg_1 423 426 PF00400 0.131
TRG_ER_diArg_1 445 447 PF00400 0.557
TRG_ER_diArg_1 93 95 PF00400 0.494
TRG_NLS_MonoExtC_3 378 384 PF00514 0.359
TRG_Pf-PMV_PEXEL_1 275 279 PF00026 0.467
TRG_Pf-PMV_PEXEL_1 287 292 PF00026 0.582
TRG_Pf-PMV_PEXEL_1 446 450 PF00026 0.649

Homologs

Protein Taxonomy Sequence identity Coverage
A0A0N0P825 Leptomonas seymouri 93% 100%
A0A0S4J8L0 Bodo saltans 56% 97%
A0A1X0NQV3 Trypanosomatidae 69% 99%
A0A3R7KN38 Trypanosoma rangeli 73% 100%
A0A3S7X1P8 Leishmania donovani 100% 100%
A4HGN5 Leishmania braziliensis 98% 100%
D0A8A0 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 68% 100%
D8UYN9 Tetrahymena thermophila (strain SB210) 25% 70%
E9B001 Leishmania mexicana (strain MHOM/GT/2001/U1103) 99% 100%
O97472 Caenorhabditis briggsae 29% 71%
P22336 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) 31% 75%
P27694 Homo sapiens 35% 76%
Q01588 Xenopus laevis 33% 77%
Q10Q08 Oryza sativa subsp. japonica 35% 74%
Q19537 Caenorhabditis elegans 29% 71%
Q23696 Crithidia fasciculata 92% 100%
Q24492 Drosophila melanogaster 35% 77%
Q4Q884 Leishmania major 99% 100%
Q5FW17 Xenopus tropicalis 33% 77%
Q5R7Q4 Pongo abelii 35% 76%
Q5ZJJ2 Gallus gallus 34% 76%
Q6NY74 Danio rerio 34% 78%
Q6YZ49 Oryza sativa subsp. japonica 33% 71%
Q8VEE4 Mus musculus 34% 75%
Q92372 Schizosaccharomyces pombe (strain 972 / ATCC 24843) 33% 77%
Q9FME0 Arabidopsis thaliana 33% 74%
Q9SD82 Arabidopsis thaliana 35% 77%
Q9SKI4 Arabidopsis thaliana 32% 73%
V5DBB0 Trypanosoma cruzi 74% 100%

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LeishMANIAdb - Server version: v0.0.2. - Database version: v0.0.1. - ChangeLog - © 2022-2024 Protein Bioinformatics Research Group, Institute of Enzymology, RCNS