TRUD domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase D (TruD) - putative

Species:

Leishmania infantum

UniProt:

A4I3P1_LEIIN

TriTrypDb:

LINF_280022500

Length:

669

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4I3P1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I3P1

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	10
GO:0006139	nucleobase-containing compound metabolic process	3	10
GO:0006725	cellular aromatic compound metabolic process	3	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008152	metabolic process	1	10
GO:0009451	RNA modification	5	10
GO:0009987	cellular process	1	10
GO:0016070	RNA metabolic process	5	10
GO:0034641	cellular nitrogen compound metabolic process	3	10
GO:0043170	macromolecule metabolic process	3	10
GO:0043412	macromolecule modification	4	10
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	10
GO:0046483	heterocycle metabolic process	3	10
GO:0071704	organic substance metabolic process	2	10
GO:0090304	nucleic acid metabolic process	4	10
GO:1901360	organic cyclic compound metabolic process	3	10

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0004730	pseudouridylate synthase activity	5	2
GO:0016829	lyase activity	2	2
GO:0016835	carbon-oxygen lyase activity	3	2
GO:0016836	hydro-lyase activity	4	2
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	278	282	PF00656	0.326
CLV_NRD_NRD_1	125	127	PF00675	0.391
CLV_NRD_NRD_1	128	130	PF00675	0.387
CLV_NRD_NRD_1	246	248	PF00675	0.310
CLV_NRD_NRD_1	250	252	PF00675	0.312
CLV_NRD_NRD_1	308	310	PF00675	0.304
CLV_NRD_NRD_1	357	359	PF00675	0.445
CLV_NRD_NRD_1	433	435	PF00675	0.304
CLV_NRD_NRD_1	551	553	PF00675	0.585
CLV_NRD_NRD_1	568	570	PF00675	0.509
CLV_NRD_NRD_1	619	621	PF00675	0.541
CLV_NRD_NRD_1	622	624	PF00675	0.570
CLV_NRD_NRD_1	635	637	PF00675	0.621
CLV_PCSK_FUR_1	126	130	PF00082	0.422
CLV_PCSK_FUR_1	619	623	PF00082	0.572
CLV_PCSK_FUR_1	633	637	PF00082	0.580
CLV_PCSK_KEX2_1	125	127	PF00082	0.424
CLV_PCSK_KEX2_1	128	130	PF00082	0.426
CLV_PCSK_KEX2_1	245	247	PF00082	0.304
CLV_PCSK_KEX2_1	250	252	PF00082	0.303
CLV_PCSK_KEX2_1	308	310	PF00082	0.304
CLV_PCSK_KEX2_1	357	359	PF00082	0.396
CLV_PCSK_KEX2_1	568	570	PF00082	0.509
CLV_PCSK_KEX2_1	577	579	PF00082	0.490
CLV_PCSK_KEX2_1	611	613	PF00082	0.514
CLV_PCSK_KEX2_1	619	621	PF00082	0.485
CLV_PCSK_KEX2_1	622	624	PF00082	0.516
CLV_PCSK_KEX2_1	635	637	PF00082	0.589
CLV_PCSK_PC1ET2_1	577	579	PF00082	0.633
CLV_PCSK_PC1ET2_1	611	613	PF00082	0.525
CLV_PCSK_PC1ET2_1	621	623	PF00082	0.520
CLV_PCSK_PC7_1	246	252	PF00082	0.304
CLV_PCSK_PC7_1	564	570	PF00082	0.513
CLV_PCSK_SKI1_1	157	161	PF00082	0.283
CLV_PCSK_SKI1_1	167	171	PF00082	0.391
CLV_PCSK_SKI1_1	227	231	PF00082	0.318
CLV_PCSK_SKI1_1	308	312	PF00082	0.314
CLV_PCSK_SKI1_1	453	457	PF00082	0.430
CLV_PCSK_SKI1_1	50	54	PF00082	0.709
CLV_PCSK_SKI1_1	577	581	PF00082	0.606
CLV_Separin_Metazoa	427	431	PF03568	0.304
DEG_APCC_DBOX_1	108	116	PF00400	0.319
DEG_APCC_DBOX_1	156	164	PF00400	0.340
DOC_CDC14_PxL_1	262	270	PF14671	0.316
DOC_CYCLIN_RxL_1	430	442	PF00134	0.389
DOC_CYCLIN_RxL_1	575	584	PF00134	0.595
DOC_MAPK_gen_1	125	136	PF00069	0.331
DOC_MAPK_gen_1	245	257	PF00069	0.381
DOC_PP1_RVXF_1	575	582	PF00149	0.653
DOC_PP2B_LxvP_1	484	487	PF13499	0.450
DOC_PP2B_LxvP_1	542	545	PF13499	0.531
DOC_PP4_FxxP_1	425	428	PF00568	0.304
DOC_USP7_MATH_1	28	32	PF00917	0.727
DOC_USP7_MATH_1	415	419	PF00917	0.425
DOC_USP7_MATH_1	528	532	PF00917	0.676
DOC_USP7_MATH_1	606	610	PF00917	0.618
DOC_WW_Pin1_4	113	118	PF00397	0.304
DOC_WW_Pin1_4	624	629	PF00397	0.658
DOC_WW_Pin1_4	636	641	PF00397	0.580
LIG_14-3-3_CanoR_1	133	141	PF00244	0.391
LIG_14-3-3_CanoR_1	190	194	PF00244	0.304
LIG_14-3-3_CanoR_1	308	315	PF00244	0.288
LIG_14-3-3_CanoR_1	635	640	PF00244	0.697
LIG_14-3-3_CanoR_1	92	97	PF00244	0.425
LIG_Actin_WH2_2	281	299	PF00022	0.406
LIG_AP2alpha_2	549	551	PF02296	0.614
LIG_BRCT_BRCA1_1	663	667	PF00533	0.608
LIG_CtBP_PxDLS_1	436	440	PF00389	0.319
LIG_deltaCOP1_diTrp_1	359	364	PF00928	0.425
LIG_eIF4E_1	141	147	PF01652	0.338
LIG_FHA_1	14	20	PF00498	0.698
LIG_FHA_1	141	147	PF00498	0.316
LIG_FHA_1	323	329	PF00498	0.319
LIG_FHA_1	478	484	PF00498	0.598
LIG_FHA_1	649	655	PF00498	0.722
LIG_FHA_2	218	224	PF00498	0.325
LIG_FHA_2	238	244	PF00498	0.455
LIG_FHA_2	258	264	PF00498	0.260
LIG_FHA_2	276	282	PF00498	0.180
LIG_FHA_2	350	356	PF00498	0.361
LIG_FHA_2	361	367	PF00498	0.348
LIG_FHA_2	375	381	PF00498	0.359
LIG_FHA_2	502	508	PF00498	0.612
LIG_FHA_2	55	61	PF00498	0.321
LIG_LIR_Apic_2	424	428	PF02991	0.304
LIG_LIR_Gen_1	199	210	PF02991	0.304
LIG_LIR_Gen_1	317	328	PF02991	0.255
LIG_LIR_Gen_1	359	368	PF02991	0.425
LIG_LIR_Gen_1	656	661	PF02991	0.586
LIG_LIR_Nem_3	179	185	PF02991	0.337
LIG_LIR_Nem_3	199	205	PF02991	0.294
LIG_LIR_Nem_3	298	303	PF02991	0.336
LIG_LIR_Nem_3	317	323	PF02991	0.154
LIG_LIR_Nem_3	359	364	PF02991	0.425
LIG_LIR_Nem_3	387	391	PF02991	0.331
LIG_LIR_Nem_3	488	494	PF02991	0.581
LIG_LIR_Nem_3	507	512	PF02991	0.384
LIG_LIR_Nem_3	656	660	PF02991	0.626
LIG_NRBOX	225	231	PF00104	0.304
LIG_PCNA_PIPBox_1	395	404	PF02747	0.338
LIG_PCNA_yPIPBox_3	395	408	PF02747	0.431
LIG_PCNA_yPIPBox_3	458	470	PF02747	0.304
LIG_Pex14_1	360	364	PF04695	0.425
LIG_Pex14_2	152	156	PF04695	0.304
LIG_Pex14_2	212	216	PF04695	0.319
LIG_SH2_CRK	402	406	PF00017	0.384
LIG_SH2_CRK	461	465	PF00017	0.304
LIG_SH2_CRK	614	618	PF00017	0.617
LIG_SH2_GRB2like	461	464	PF00017	0.304
LIG_SH2_NCK_1	443	447	PF00017	0.369
LIG_SH2_NCK_1	461	465	PF00017	0.236
LIG_SH2_SRC	461	464	PF00017	0.223
LIG_SH2_SRC	512	515	PF00017	0.651
LIG_SH2_STAP1	461	465	PF00017	0.236
LIG_SH2_STAP1	614	618	PF00017	0.617
LIG_SH2_STAT3	186	189	PF00017	0.329
LIG_SH2_STAT3	219	222	PF00017	0.316
LIG_SH2_STAT3	401	404	PF00017	0.319
LIG_SH2_STAT5	102	105	PF00017	0.319
LIG_SH2_STAT5	219	222	PF00017	0.306
LIG_SH2_STAT5	300	303	PF00017	0.380
LIG_SH2_STAT5	376	379	PF00017	0.389
LIG_SH2_STAT5	501	504	PF00017	0.534
LIG_SH2_STAT5	64	67	PF00017	0.304
LIG_SH3_3	142	148	PF00018	0.300
LIG_SH3_3	160	166	PF00018	0.304
LIG_SH3_3	378	384	PF00018	0.304
LIG_SH3_3	425	431	PF00018	0.304
LIG_SH3_3	599	605	PF00018	0.512
LIG_SUMO_SIM_anti_2	479	485	PF11976	0.500
LIG_SUMO_SIM_par_1	435	440	PF11976	0.446
LIG_WRC_WIRS_1	315	320	PF05994	0.425
LIG_WRC_WIRS_1	361	366	PF05994	0.418
LIG_WRC_WIRS_1	654	659	PF05994	0.686
LIG_WW_3	427	431	PF00397	0.304
MOD_CDK_SPK_2	624	629	PF00069	0.658
MOD_CK1_1	137	143	PF00069	0.318
MOD_CK1_1	4	10	PF00069	0.741
MOD_CK1_1	584	590	PF00069	0.548
MOD_CK1_1	642	648	PF00069	0.715
MOD_CK1_1	650	656	PF00069	0.624
MOD_CK2_1	222	228	PF00069	0.328
MOD_CK2_1	237	243	PF00069	0.344
MOD_CK2_1	28	34	PF00069	0.612
MOD_CK2_1	349	355	PF00069	0.418
MOD_CK2_1	526	532	PF00069	0.636
MOD_CK2_1	54	60	PF00069	0.419
MOD_DYRK1A_RPxSP_1	636	640	PF00069	0.708
MOD_GlcNHglycan	16	19	PF01048	0.588
MOD_GlcNHglycan	273	276	PF01048	0.373
MOD_GlcNHglycan	3	6	PF01048	0.730
MOD_GlcNHglycan	30	33	PF01048	0.568
MOD_GlcNHglycan	41	44	PF01048	0.641
MOD_GlcNHglycan	419	422	PF01048	0.389
MOD_GlcNHglycan	471	474	PF01048	0.480
MOD_GlcNHglycan	528	531	PF01048	0.629
MOD_GlcNHglycan	608	611	PF01048	0.612
MOD_GlcNHglycan	647	650	PF01048	0.740
MOD_GSK3_1	271	278	PF00069	0.363
MOD_GSK3_1	28	35	PF00069	0.667
MOD_GSK3_1	304	311	PF00069	0.338
MOD_GSK3_1	4	11	PF00069	0.730
MOD_GSK3_1	41	48	PF00069	0.670
MOD_GSK3_1	526	533	PF00069	0.687
MOD_GSK3_1	580	587	PF00069	0.512
MOD_GSK3_1	634	641	PF00069	0.767
MOD_GSK3_1	644	651	PF00069	0.732
MOD_GSK3_1	656	663	PF00069	0.561
MOD_LATS_1	90	96	PF00433	0.425
MOD_N-GLC_1	113	118	PF02516	0.297
MOD_N-GLC_1	217	222	PF02516	0.379
MOD_N-GLC_1	606	611	PF02516	0.625
MOD_NEK2_1	217	222	PF00069	0.266
MOD_NEK2_1	291	296	PF00069	0.290
MOD_NEK2_1	469	474	PF00069	0.549
MOD_NEK2_1	580	585	PF00069	0.563
MOD_NEK2_1	634	639	PF00069	0.715
MOD_NEK2_1	65	70	PF00069	0.449
MOD_NEK2_1	660	665	PF00069	0.473
MOD_NEK2_2	448	453	PF00069	0.446
MOD_OFUCOSY	40	45	PF10250	0.543
MOD_PIKK_1	237	243	PF00454	0.446
MOD_PIKK_1	291	297	PF00454	0.305
MOD_PIKK_1	556	562	PF00454	0.598
MOD_PIKK_1	584	590	PF00454	0.611
MOD_PK_1	161	167	PF00069	0.446
MOD_PK_1	92	98	PF00069	0.396
MOD_PKA_1	308	314	PF00069	0.304
MOD_PKA_1	635	641	PF00069	0.723
MOD_PKA_2	14	20	PF00069	0.649
MOD_PKA_2	189	195	PF00069	0.304
MOD_PKA_2	308	314	PF00069	0.292
MOD_PKA_2	366	372	PF00069	0.444
MOD_PKA_2	374	380	PF00069	0.122
MOD_PKA_2	54	60	PF00069	0.455
MOD_PKA_2	563	569	PF00069	0.601
MOD_PKA_2	634	640	PF00069	0.686
MOD_PKB_1	633	641	PF00069	0.736
MOD_Plk_1	137	143	PF00069	0.318
MOD_Plk_1	161	167	PF00069	0.417
MOD_Plk_1	222	228	PF00069	0.338
MOD_Plk_1	437	443	PF00069	0.415
MOD_Plk_1	581	587	PF00069	0.650
MOD_Plk_1	660	666	PF00069	0.471
MOD_Plk_1	8	14	PF00069	0.647
MOD_Plk_2-3	54	60	PF00069	0.321
MOD_Plk_4	314	320	PF00069	0.313
MOD_Plk_4	479	485	PF00069	0.533
MOD_ProDKin_1	113	119	PF00069	0.304
MOD_ProDKin_1	624	630	PF00069	0.663
MOD_ProDKin_1	636	642	PF00069	0.577
MOD_SUMO_for_1	46	49	PF00179	0.627
MOD_SUMO_rev_2	526	535	PF00179	0.623
MOD_SUMO_rev_2	570	579	PF00179	0.596
TRG_DiLeu_BaEn_1	537	542	PF01217	0.474
TRG_DiLeu_BaLyEn_6	575	580	PF01217	0.658
TRG_ENDOCYTIC_2	300	303	PF00928	0.362
TRG_ENDOCYTIC_2	402	405	PF00928	0.334
TRG_ENDOCYTIC_2	461	464	PF00928	0.318
TRG_ENDOCYTIC_2	614	617	PF00928	0.617
TRG_ER_diArg_1	109	112	PF00400	0.304
TRG_ER_diArg_1	125	127	PF00400	0.393
TRG_ER_diArg_1	244	247	PF00400	0.304
TRG_ER_diArg_1	249	251	PF00400	0.303
TRG_ER_diArg_1	308	310	PF00400	0.304
TRG_ER_diArg_1	373	376	PF00400	0.446
TRG_ER_diArg_1	618	620	PF00400	0.502
TRG_ER_diArg_1	622	624	PF00400	0.552
TRG_ER_diArg_1	632	635	PF00400	0.655
TRG_NES_CRM1_1	101	113	PF08389	0.361
TRG_NLS_Bipartite_1	611	625	PF00514	0.593
TRG_NLS_MonoCore_2	619	624	PF00514	0.653
TRG_NLS_MonoExtN_4	619	625	PF00514	0.625
TRG_Pf-PMV_PEXEL_1	224	228	PF00026	0.308
TRG_Pf-PMV_PEXEL_1	308	313	PF00026	0.319
TRG_Pf-PMV_PEXEL_1	335	340	PF00026	0.456
TRG_Pf-PMV_PEXEL_1	474	479	PF00026	0.570
TRG_Pf-PMV_PEXEL_1	578	582	PF00026	0.608

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P820	Leptomonas seymouri	75%	67%
A0A0S4K0L8	Bodo saltans	45%	66%
A0A1X0NQX7	Trypanosomatidae	57%	73%
A0A3Q8II50	Leishmania donovani	99%	79%
A0A3S5ISD4	Trypanosoma rangeli	59%	76%
A4HGL5	Leishmania braziliensis	89%	100%
D0A8C3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	72%
E9AZY2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
O74343	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	23%	98%
Q08DI8	Bos taurus	23%	100%
Q1L8I0	Danio rerio	26%	100%
Q4Q8A3	Leishmania major	95%	100%
Q8CE46	Mus musculus	26%	95%
Q91VU7	Mus musculus	24%	100%
Q96PZ0	Homo sapiens	24%	100%
Q9H0K6	Homo sapiens	28%	95%
V5BFB8	Trypanosoma cruzi	60%	73%