NADH-u_ox-rdase domain-containing protein

Quick info Annotations Localization Abundance Expansion Sequence features Structure Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Domain of unknown function (DUF543) - putative

Species:

Leishmania infantum

UniProt:

A4I2U5_LEIIN

TriTrypDb:

LINF_270022100

Length:

108

Annotations

LeishMANIAdb annotations

Likely part of the mitochondrial MICOS complex, based on homology.. It is possible that this protein only has a single TM segment as the first can also be a transit signal.. Localization: Mitochondrial inner membrane (by homology)

Annotations by Jardim et al.

Uncharacterized Protein, Uncharacterized

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0032991	protein-containing complex	1	11
GO:0061617	MICOS complex	4	11
GO:0098796	membrane protein complex	2	11
GO:0098798	mitochondrial protein-containing complex	2	11
GO:0098800	inner mitochondrial membrane protein complex	3	11
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A4I2U5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I2U5

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	29	31	PF00675	0.246
CLV_PCSK_KEX2_1	84	86	PF00082	0.476
CLV_PCSK_PC1ET2_1	84	86	PF00082	0.512
CLV_PCSK_SKI1_1	73	77	PF00082	0.273
DEG_Kelch_Keap1_1	14	19	PF01344	0.163
DOC_MAPK_gen_1	30	36	PF00069	0.214
DOC_MAPK_gen_1	84	92	PF00069	0.469
DOC_MAPK_MEF2A_6	84	92	PF00069	0.469
DOC_USP7_MATH_1	11	15	PF00917	0.309
DOC_USP7_MATH_1	51	55	PF00917	0.306
DOC_WW_Pin1_4	1	6	PF00397	0.523
DOC_WW_Pin1_4	46	51	PF00397	0.257
LIG_14-3-3_CanoR_1	30	36	PF00244	0.263
LIG_BIR_II_1	1	5	PF00653	0.673
LIG_FHA_1	10	16	PF00498	0.341
LIG_SH2_STAT5	74	77	PF00017	0.262
LIG_SH3_3	2	8	PF00018	0.703
LIG_SUMO_SIM_par_1	54	61	PF11976	0.306
MOD_CDK_SPK_2	1	6	PF00069	0.501
MOD_CDK_SPxxK_3	46	53	PF00069	0.257
MOD_CK1_1	24	30	PF00069	0.287
MOD_CK1_1	9	15	PF00069	0.323
MOD_CK2_1	13	19	PF00069	0.217
MOD_GlcNHglycan	1	4	PF01048	0.594
MOD_GlcNHglycan	16	19	PF01048	0.275
MOD_GlcNHglycan	37	40	PF01048	0.233
MOD_GlcNHglycan	53	56	PF01048	0.315
MOD_GlcNHglycan	64	67	PF01048	0.285
MOD_GSK3_1	58	65	PF00069	0.298
MOD_GSK3_1	6	13	PF00069	0.296
MOD_PIKK_1	95	101	PF00454	0.598
MOD_PKA_1	30	36	PF00069	0.230
MOD_PKA_2	29	35	PF00069	0.259
MOD_Plk_1	21	27	PF00069	0.229
MOD_Plk_4	24	30	PF00069	0.378
MOD_ProDKin_1	1	7	PF00069	0.524
MOD_ProDKin_1	46	52	PF00069	0.257
MOD_SUMO_rev_2	97	107	PF00179	0.522
TRG_Pf-PMV_PEXEL_1	73	77	PF00026	0.246
TRG_Pf-PMV_PEXEL_1	85	89	PF00026	0.462

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PD22	Leptomonas seymouri	80%	100%
A0A3Q8IHN0	Leishmania donovani	99%	100%
A4HBS0	Leishmania braziliensis	28%	100%
A4HFT1	Leishmania braziliensis	89%	100%
D0A1G4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	95%
D0A621	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	58%	100%
E9ADD2	Leishmania major	96%	100%
E9AV41	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9AZ57	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%