Cleavage and polyadenylation specificity factor subunit 2

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cleavage and polyadenylation specificity factor - putative

Species:

Leishmania infantum

UniProt:

A4I159_LEIIN

TriTrypDb:

LINF_250006800

Length:

819

Annotations

Annotations by Jardim et al.

RNA Processing, cleavage and polyadenylation specificity factor

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005847	mRNA cleavage and polyadenylation specificity factor complex	4	11
GO:0005849	mRNA cleavage factor complex	3	11
GO:0032991	protein-containing complex	1	11
GO:0140513	nuclear protein-containing complex	2	11
GO:0005654	nucleoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4I159

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I159

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006378	mRNA polyadenylation	7	11
GO:0006379	mRNA cleavage	7	11
GO:0006396	RNA processing	6	11
GO:0006397	mRNA processing	7	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0016071	mRNA metabolic process	6	11
GO:0031123	RNA 3'-end processing	7	11
GO:0031124	mRNA 3'-end processing	8	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043631	RNA polyadenylation	6	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:0090305	nucleic acid phosphodiester bond hydrolysis	5	11
GO:0090501	RNA phosphodiester bond hydrolysis	6	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:0006398	mRNA 3'-end processing by stem-loop binding and cleavage	8	1
GO:0008334	histone mRNA metabolic process	7	1
GO:0098787	mRNA cleavage involved in mRNA processing	8	1
GO:0098789	pre-mRNA cleavage required for polyadenylation	9	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0005488	binding	1	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	118	122	PF00656	0.460
CLV_NRD_NRD_1	392	394	PF00675	0.416
CLV_NRD_NRD_1	416	418	PF00675	0.445
CLV_NRD_NRD_1	579	581	PF00675	0.364
CLV_NRD_NRD_1	676	678	PF00675	0.335
CLV_NRD_NRD_1	713	715	PF00675	0.336
CLV_NRD_NRD_1	755	757	PF00675	0.314
CLV_NRD_NRD_1	786	788	PF00675	0.250
CLV_NRD_NRD_1	808	810	PF00675	0.271
CLV_PCSK_KEX2_1	307	309	PF00082	0.414
CLV_PCSK_KEX2_1	392	394	PF00082	0.392
CLV_PCSK_KEX2_1	415	417	PF00082	0.445
CLV_PCSK_KEX2_1	519	521	PF00082	0.660
CLV_PCSK_KEX2_1	539	541	PF00082	0.383
CLV_PCSK_KEX2_1	579	581	PF00082	0.448
CLV_PCSK_KEX2_1	676	678	PF00082	0.267
CLV_PCSK_KEX2_1	712	714	PF00082	0.271
CLV_PCSK_KEX2_1	755	757	PF00082	0.471
CLV_PCSK_KEX2_1	808	810	PF00082	0.382
CLV_PCSK_PC1ET2_1	307	309	PF00082	0.414
CLV_PCSK_PC1ET2_1	519	521	PF00082	0.521
CLV_PCSK_PC1ET2_1	539	541	PF00082	0.383
CLV_PCSK_SKI1_1	230	234	PF00082	0.370
CLV_PCSK_SKI1_1	276	280	PF00082	0.418
CLV_PCSK_SKI1_1	307	311	PF00082	0.444
CLV_PCSK_SKI1_1	34	38	PF00082	0.227
CLV_PCSK_SKI1_1	422	426	PF00082	0.530
CLV_PCSK_SKI1_1	460	464	PF00082	0.486
CLV_PCSK_SKI1_1	482	486	PF00082	0.757
CLV_PCSK_SKI1_1	582	586	PF00082	0.421
CLV_PCSK_SKI1_1	598	602	PF00082	0.212
CLV_PCSK_SKI1_1	622	626	PF00082	0.347
CLV_PCSK_SKI1_1	632	636	PF00082	0.330
DEG_APCC_DBOX_1	581	589	PF00400	0.442
DEG_Nend_Nbox_1	1	3	PF02207	0.416
DEG_SPOP_SBC_1	640	644	PF00917	0.309
DOC_ANK_TNKS_1	786	793	PF00023	0.382
DOC_CKS1_1	799	804	PF01111	0.382
DOC_CYCLIN_RxL_1	224	235	PF00134	0.406
DOC_CYCLIN_RxL_1	496	508	PF00134	0.581
DOC_CYCLIN_yCln2_LP_2	491	497	PF00134	0.536
DOC_MAPK_gen_1	480	489	PF00069	0.558
DOC_MAPK_gen_1	569	577	PF00069	0.516
DOC_MAPK_gen_1	579	585	PF00069	0.398
DOC_MAPK_gen_1	773	780	PF00069	0.382
DOC_MAPK_HePTP_8	252	264	PF00069	0.404
DOC_MAPK_MEF2A_6	241	250	PF00069	0.315
DOC_MAPK_MEF2A_6	255	264	PF00069	0.262
DOC_MAPK_MEF2A_6	482	491	PF00069	0.592
DOC_MAPK_MEF2A_6	569	577	PF00069	0.476
DOC_MAPK_MEF2A_6	773	780	PF00069	0.267
DOC_MAPK_RevD_3	775	788	PF00069	0.382
DOC_PP1_RVXF_1	32	38	PF00149	0.509
DOC_PP1_RVXF_1	498	505	PF00149	0.548
DOC_PP2B_LxvP_1	489	492	PF13499	0.537
DOC_PP2B_PxIxI_1	327	333	PF00149	0.419
DOC_PP4_FxxP_1	182	185	PF00568	0.427
DOC_PP4_FxxP_1	198	201	PF00568	0.325
DOC_USP7_MATH_1	727	731	PF00917	0.308
DOC_USP7_UBL2_3	146	150	PF12436	0.509
DOC_USP7_UBL2_3	230	234	PF12436	0.396
DOC_USP7_UBL2_3	598	602	PF12436	0.455
DOC_WW_Pin1_4	16	21	PF00397	0.459
DOC_WW_Pin1_4	64	69	PF00397	0.427
DOC_WW_Pin1_4	798	803	PF00397	0.259
DOC_WW_Pin1_4	81	86	PF00397	0.427
LIG_14-3-3_CanoR_1	3	11	PF00244	0.302
LIG_14-3-3_CanoR_1	544	551	PF00244	0.549
LIG_14-3-3_CanoR_1	582	592	PF00244	0.332
LIG_14-3-3_CanoR_1	712	720	PF00244	0.314
LIG_14-3-3_CanoR_1	808	812	PF00244	0.292
LIG_Actin_WH2_2	225	243	PF00022	0.348
LIG_Actin_WH2_2	660	678	PF00022	0.314
LIG_Actin_WH2_2	745	760	PF00022	0.277
LIG_Actin_WH2_2	792	810	PF00022	0.267
LIG_APCC_ABBA_1	777	782	PF00400	0.382
LIG_BIR_III_2	548	552	PF00653	0.671
LIG_BRCT_BRCA1_1	113	117	PF00533	0.470
LIG_BRCT_BRCA1_1	210	214	PF00533	0.436
LIG_BRCT_BRCA1_1	336	340	PF00533	0.440
LIG_BRCT_BRCA1_1	637	641	PF00533	0.411
LIG_BRCT_BRCA1_1	7	11	PF00533	0.345
LIG_BRCT_BRCA1_1	743	747	PF00533	0.382
LIG_Clathr_ClatBox_1	27	31	PF01394	0.427
LIG_Clathr_ClatBox_1	777	781	PF01394	0.329
LIG_CtBP_PxDLS_1	649	653	PF00389	0.378
LIG_deltaCOP1_diTrp_1	38	45	PF00928	0.427
LIG_eIF4E_1	176	182	PF01652	0.427
LIG_FHA_1	114	120	PF00498	0.424
LIG_FHA_1	124	130	PF00498	0.427
LIG_FHA_1	201	207	PF00498	0.346
LIG_FHA_1	293	299	PF00498	0.338
LIG_FHA_1	45	51	PF00498	0.509
LIG_FHA_1	484	490	PF00498	0.559
LIG_FHA_1	508	514	PF00498	0.570
LIG_FHA_1	522	528	PF00498	0.598
LIG_FHA_1	684	690	PF00498	0.354
LIG_FHA_1	791	797	PF00498	0.382
LIG_FHA_1	799	805	PF00498	0.382
LIG_FHA_1	93	99	PF00498	0.450
LIG_FHA_2	116	122	PF00498	0.548
LIG_FHA_2	221	227	PF00498	0.442
LIG_FHA_2	291	297	PF00498	0.364
LIG_FHA_2	733	739	PF00498	0.306
LIG_GBD_Chelix_1	667	675	PF00786	0.382
LIG_KLC1_Yacidic_2	402	407	PF13176	0.531
LIG_LIR_Apic_2	106	112	PF02991	0.460
LIG_LIR_Apic_2	179	185	PF02991	0.427
LIG_LIR_Apic_2	522	528	PF02991	0.485
LIG_LIR_Gen_1	130	139	PF02991	0.414
LIG_LIR_Gen_1	300	309	PF02991	0.415
LIG_LIR_Gen_1	402	412	PF02991	0.417
LIG_LIR_Gen_1	423	432	PF02991	0.652
LIG_LIR_Gen_1	765	776	PF02991	0.382
LIG_LIR_Gen_1	8	15	PF02991	0.357
LIG_LIR_Nem_3	130	136	PF02991	0.414
LIG_LIR_Nem_3	273	278	PF02991	0.334
LIG_LIR_Nem_3	300	304	PF02991	0.508
LIG_LIR_Nem_3	402	408	PF02991	0.413
LIG_LIR_Nem_3	423	428	PF02991	0.645
LIG_LIR_Nem_3	503	507	PF02991	0.621
LIG_LIR_Nem_3	765	771	PF02991	0.382
LIG_LIR_Nem_3	8	14	PF02991	0.339
LIG_LIR_Nem_3	810	815	PF02991	0.268
LIG_Pex14_1	172	176	PF04695	0.414
LIG_Pex14_1	41	45	PF04695	0.427
LIG_Pex14_2	37	41	PF04695	0.509
LIG_Pex14_2	45	49	PF04695	0.509
LIG_Rb_pABgroove_1	31	39	PF01858	0.509
LIG_RPA_C_Fungi	350	362	PF08784	0.490
LIG_SH2_CRK	109	113	PF00017	0.382
LIG_SH2_CRK	24	28	PF00017	0.385
LIG_SH2_CRK	275	279	PF00017	0.306
LIG_SH2_CRK	525	529	PF00017	0.487
LIG_SH2_NCK_1	720	724	PF00017	0.270
LIG_SH2_PTP2	405	408	PF00017	0.478
LIG_SH2_SRC	109	112	PF00017	0.382
LIG_SH2_STAP1	170	174	PF00017	0.314
LIG_SH2_STAP1	225	229	PF00017	0.360
LIG_SH2_STAP1	24	28	PF00017	0.395
LIG_SH2_STAP1	657	661	PF00017	0.406
LIG_SH2_STAP1	815	819	PF00017	0.504
LIG_SH2_STAT3	225	228	PF00017	0.371
LIG_SH2_STAT3	659	662	PF00017	0.402
LIG_SH2_STAT5	183	186	PF00017	0.276
LIG_SH2_STAT5	26	29	PF00017	0.327
LIG_SH2_STAT5	301	304	PF00017	0.406
LIG_SH2_STAT5	349	352	PF00017	0.369
LIG_SH2_STAT5	405	408	PF00017	0.478
LIG_SH3_1	52	58	PF00018	0.270
LIG_SH3_3	17	23	PF00018	0.377
LIG_SH3_3	243	249	PF00018	0.285
LIG_SH3_3	364	370	PF00018	0.370
LIG_SH3_3	487	493	PF00018	0.690
LIG_SH3_3	52	58	PF00018	0.270
LIG_SUMO_SIM_anti_2	359	366	PF11976	0.408
LIG_SUMO_SIM_par_1	26	31	PF11976	0.249
LIG_SUMO_SIM_par_1	359	366	PF11976	0.412
LIG_SUMO_SIM_par_1	648	654	PF11976	0.503
LIG_SUMO_SIM_par_1	775	782	PF11976	0.327
LIG_SUMO_SIM_par_1	791	798	PF11976	0.327
LIG_TRAF2_1	318	321	PF00917	0.344
LIG_TRAF2_1	397	400	PF00917	0.520
LIG_TRAF2_1	530	533	PF00917	0.578
LIG_TRAF2_1	761	764	PF00917	0.459
LIG_TRFH_1	193	197	PF08558	0.338
LIG_WRC_WIRS_1	104	109	PF05994	0.314
LIG_WRC_WIRS_1	133	138	PF05994	0.327
LIG_WW_2	367	370	PF00397	0.512
MOD_CK1_1	135	141	PF00069	0.314
MOD_CK1_1	219	225	PF00069	0.476
MOD_CK1_1	394	400	PF00069	0.554
MOD_CK1_1	48	54	PF00069	0.193
MOD_CK1_1	5	11	PF00069	0.288
MOD_CK1_1	511	517	PF00069	0.569
MOD_CK1_1	568	574	PF00069	0.484
MOD_CK1_1	586	592	PF00069	0.433
MOD_CK1_1	642	648	PF00069	0.432
MOD_CK1_1	683	689	PF00069	0.290
MOD_CK1_1	721	727	PF00069	0.269
MOD_CK1_1	798	804	PF00069	0.277
MOD_CK1_1	81	87	PF00069	0.426
MOD_CK2_1	10	16	PF00069	0.462
MOD_CK2_1	135	141	PF00069	0.382
MOD_CK2_1	220	226	PF00069	0.451
MOD_CK2_1	290	296	PF00069	0.352
MOD_CK2_1	315	321	PF00069	0.322
MOD_CK2_1	394	400	PF00069	0.514
MOD_CK2_1	527	533	PF00069	0.537
MOD_CK2_1	682	688	PF00069	0.360
MOD_CK2_1	732	738	PF00069	0.306
MOD_CK2_1	758	764	PF00069	0.459
MOD_GlcNHglycan	317	320	PF01048	0.465
MOD_GlcNHglycan	342	345	PF01048	0.315
MOD_GlcNHglycan	396	399	PF01048	0.444
MOD_GlcNHglycan	4	7	PF01048	0.294
MOD_GlcNHglycan	422	425	PF01048	0.549
MOD_GlcNHglycan	513	516	PF01048	0.472
MOD_GlcNHglycan	587	591	PF01048	0.345
MOD_GlcNHglycan	616	619	PF01048	0.455
MOD_GlcNHglycan	695	699	PF01048	0.437
MOD_GlcNHglycan	743	746	PF01048	0.382
MOD_GlcNHglycan	764	767	PF01048	0.323
MOD_GSK3_1	111	118	PF00069	0.202
MOD_GSK3_1	128	135	PF00069	0.128
MOD_GSK3_1	185	192	PF00069	0.249
MOD_GSK3_1	216	223	PF00069	0.470
MOD_GSK3_1	44	51	PF00069	0.301
MOD_GSK3_1	496	503	PF00069	0.645
MOD_GSK3_1	5	12	PF00069	0.287
MOD_GSK3_1	507	514	PF00069	0.675
MOD_GSK3_1	596	603	PF00069	0.351
MOD_GSK3_1	635	642	PF00069	0.564
MOD_GSK3_1	64	71	PF00069	0.327
MOD_GSK3_1	651	658	PF00069	0.392
MOD_GSK3_1	663	670	PF00069	0.353
MOD_GSK3_1	678	685	PF00069	0.169
MOD_GSK3_1	723	730	PF00069	0.363
MOD_GSK3_1	758	765	PF00069	0.382
MOD_GSK3_1	790	797	PF00069	0.259
MOD_GSK3_1	99	106	PF00069	0.314
MOD_LATS_1	498	504	PF00433	0.595
MOD_N-GLC_1	219	224	PF02516	0.499
MOD_NEK2_1	2	7	PF00069	0.338
MOD_NEK2_1	208	213	PF00069	0.352
MOD_NEK2_1	214	219	PF00069	0.408
MOD_NEK2_1	236	241	PF00069	0.393
MOD_NEK2_1	388	393	PF00069	0.380
MOD_NEK2_1	45	50	PF00069	0.327
MOD_NEK2_1	507	512	PF00069	0.568
MOD_NEK2_1	641	646	PF00069	0.427
MOD_NEK2_1	663	668	PF00069	0.286
MOD_NEK2_1	757	762	PF00069	0.292
MOD_NEK2_1	78	83	PF00069	0.459
MOD_NEK2_1	807	812	PF00069	0.313
MOD_NEK2_2	145	150	PF00069	0.269
MOD_NEK2_2	185	190	PF00069	0.213
MOD_PIKK_1	727	733	PF00454	0.375
MOD_PIKK_1	813	819	PF00454	0.380
MOD_PKA_1	712	718	PF00069	0.382
MOD_PKA_2	2	8	PF00069	0.327
MOD_PKA_2	220	226	PF00069	0.451
MOD_PKA_2	391	397	PF00069	0.451
MOD_PKA_2	614	620	PF00069	0.369
MOD_PKA_2	678	684	PF00069	0.336
MOD_PKA_2	712	718	PF00069	0.382
MOD_PKA_2	757	763	PF00069	0.304
MOD_PKA_2	807	813	PF00069	0.292
MOD_PKB_1	756	764	PF00069	0.382
MOD_Plk_1	185	191	PF00069	0.252
MOD_Plk_1	45	51	PF00069	0.298
MOD_Plk_1	521	527	PF00069	0.484
MOD_Plk_1	663	669	PF00069	0.382
MOD_Plk_2-3	334	340	PF00069	0.322
MOD_Plk_4	10	16	PF00069	0.323
MOD_Plk_4	128	134	PF00069	0.465
MOD_Plk_4	168	174	PF00069	0.416
MOD_Plk_4	45	51	PF00069	0.292
MOD_Plk_4	596	602	PF00069	0.407
MOD_Plk_4	743	749	PF00069	0.327
MOD_Plk_4	84	90	PF00069	0.382
MOD_Plk_4	99	105	PF00069	0.382
MOD_ProDKin_1	16	22	PF00069	0.452
MOD_ProDKin_1	64	70	PF00069	0.267
MOD_ProDKin_1	798	804	PF00069	0.259
MOD_ProDKin_1	81	87	PF00069	0.267
MOD_SUMO_for_1	437	440	PF00179	0.602
MOD_SUMO_rev_2	300	309	PF00179	0.567
TRG_DiLeu_BaEn_1	532	537	PF01217	0.416
TRG_DiLeu_BaEn_4	532	538	PF01217	0.593
TRG_DiLeu_BaLyEn_6	773	778	PF01217	0.382
TRG_ENDOCYTIC_2	133	136	PF00928	0.267
TRG_ENDOCYTIC_2	170	173	PF00928	0.257
TRG_ENDOCYTIC_2	24	27	PF00928	0.396
TRG_ENDOCYTIC_2	275	278	PF00928	0.310
TRG_ENDOCYTIC_2	301	304	PF00928	0.406
TRG_ENDOCYTIC_2	405	408	PF00928	0.418
TRG_ENDOCYTIC_2	768	771	PF00928	0.382
TRG_ER_diArg_1	392	395	PF00400	0.424
TRG_ER_diArg_1	415	417	PF00400	0.517
TRG_ER_diArg_1	579	582	PF00400	0.451
TRG_ER_diArg_1	675	677	PF00400	0.292
TRG_ER_diArg_1	712	714	PF00400	0.295
TRG_ER_diArg_1	754	756	PF00400	0.427
TRG_ER_diArg_1	807	809	PF00400	0.382
TRG_Pf-PMV_PEXEL_1	137	141	PF00026	0.360
TRG_Pf-PMV_PEXEL_1	34	38	PF00026	0.249
TRG_Pf-PMV_PEXEL_1	755	759	PF00026	0.314

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEP3	Leptomonas seymouri	75%	99%
A0A0S4JJR9	Bodo saltans	30%	87%
A0A1X0P5N3	Trypanosomatidae	51%	100%
A0A3Q8IBL1	Leishmania donovani	100%	100%
A0A422NF58	Trypanosoma rangeli	51%	100%
A4HDW4	Leishmania braziliensis	89%	100%
A8XUS3	Caenorhabditis briggsae	23%	97%
D0A5E4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9AX93	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
O35218	Mus musculus	22%	100%
Q4QA79	Leishmania major	95%	100%
Q652P4	Oryza sativa subsp. japonica	23%	100%