SpoU_methylase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Protein of unknown function (DUF2946), putative

Species:

Leishmania infantum

UniProt:

A4I142_LEIIN

TriTrypDb:

Length:

468

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4I142

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I142

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0001510	RNA methylation	4	1
GO:0002128	tRNA nucleoside ribose methylation	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0008033	tRNA processing	8	1
GO:0009451	RNA modification	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	10
GO:0003723	RNA binding	4	10
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	10
GO:0008168	methyltransferase activity	4	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	10
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	10
GO:0008171	O-methyltransferase activity	5	1
GO:0008175	tRNA methyltransferase activity	5	1
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	1
GO:0016300	tRNA (uracil) methyltransferase activity	6	1
GO:0016427	tRNA (cytosine) methyltransferase activity	6	1
GO:0052665	tRNA (uracil-2'-O-)-methyltransferase activity	7	1
GO:0052666	tRNA (cytosine-2'-O-)-methyltransferase activity	7	1
GO:0062105	RNA 2'-O-methyltransferase activity	5	1
GO:0106050	tRNA 2'-O-methyltransferase activity	6	1
GO:0140101	catalytic activity, acting on a tRNA	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	148	152	PF00656	0.555
CLV_NRD_NRD_1	242	244	PF00675	0.411
CLV_NRD_NRD_1	283	285	PF00675	0.441
CLV_NRD_NRD_1	287	289	PF00675	0.475
CLV_NRD_NRD_1	335	337	PF00675	0.678
CLV_NRD_NRD_1	400	402	PF00675	0.536
CLV_PCSK_KEX2_1	241	243	PF00082	0.406
CLV_PCSK_KEX2_1	282	284	PF00082	0.467
CLV_PCSK_KEX2_1	287	289	PF00082	0.476
CLV_PCSK_KEX2_1	399	401	PF00082	0.514
CLV_PCSK_KEX2_1	7	9	PF00082	0.536
CLV_PCSK_PC1ET2_1	399	401	PF00082	0.514
CLV_PCSK_PC1ET2_1	7	9	PF00082	0.553
CLV_PCSK_PC7_1	283	289	PF00082	0.507
CLV_PCSK_SKI1_1	103	107	PF00082	0.279
CLV_PCSK_SKI1_1	235	239	PF00082	0.433
CLV_PCSK_SKI1_1	288	292	PF00082	0.503
CLV_PCSK_SKI1_1	311	315	PF00082	0.500
CLV_PCSK_SKI1_1	381	385	PF00082	0.500
CLV_PCSK_SKI1_1	404	408	PF00082	0.478
CLV_PCSK_SKI1_1	85	89	PF00082	0.379
DEG_APCC_DBOX_1	399	407	PF00400	0.510
DEG_Nend_Nbox_1	1	3	PF02207	0.621
DEG_ODPH_VHL_1	197	209	PF01847	0.458
DOC_CDC14_PxL_1	323	331	PF14671	0.444
DOC_CYCLIN_RxL_1	308	318	PF00134	0.529
DOC_CYCLIN_yClb1_LxF_4	83	88	PF00134	0.579
DOC_CYCLIN_yCln2_LP_2	321	327	PF00134	0.501
DOC_MAPK_gen_1	241	249	PF00069	0.394
DOC_MAPK_gen_1	399	410	PF00069	0.466
DOC_MAPK_gen_1	99	106	PF00069	0.494
DOC_MAPK_HePTP_8	398	410	PF00069	0.498
DOC_MAPK_MEF2A_6	242	251	PF00069	0.385
DOC_MAPK_MEF2A_6	401	410	PF00069	0.497
DOC_MAPK_MEF2A_6	72	81	PF00069	0.562
DOC_MAPK_MEF2A_6	99	106	PF00069	0.557
DOC_PP1_RVXF_1	33	39	PF00149	0.448
DOC_PP1_RVXF_1	83	89	PF00149	0.579
DOC_PP2B_LxvP_1	408	411	PF13499	0.371
DOC_USP7_MATH_1	177	181	PF00917	0.560
DOC_USP7_MATH_1	340	344	PF00917	0.584
DOC_WW_Pin1_4	104	109	PF00397	0.485
DOC_WW_Pin1_4	209	214	PF00397	0.720
DOC_WW_Pin1_4	27	32	PF00397	0.523
DOC_WW_Pin1_4	413	418	PF00397	0.414
DOC_WW_Pin1_4	430	435	PF00397	0.430
DOC_WW_Pin1_4	71	76	PF00397	0.489
LIG_Actin_RPEL_3	234	253	PF02755	0.426
LIG_Actin_WH2_2	388	406	PF00022	0.505
LIG_Actin_WH2_2	446	461	PF00022	0.413
LIG_APCC_ABBA_1	251	256	PF00400	0.392
LIG_BIR_III_4	15	19	PF00653	0.544
LIG_BRCT_BRCA1_1	343	347	PF00533	0.551
LIG_BRCT_BRCA1_1	432	436	PF00533	0.475
LIG_deltaCOP1_diTrp_1	118	127	PF00928	0.579
LIG_deltaCOP1_diTrp_1	15	23	PF00928	0.405
LIG_EH1_1	113	121	PF00400	0.454
LIG_eIF4E_1	47	53	PF01652	0.473
LIG_FHA_1	291	297	PF00498	0.404
LIG_FHA_1	31	37	PF00498	0.496
LIG_FHA_1	462	468	PF00498	0.375
LIG_FHA_2	302	308	PF00498	0.456
LIG_FHA_2	359	365	PF00498	0.349
LIG_FXI_DFP_1	11	15	PF00024	0.444
LIG_LIR_Gen_1	275	280	PF02991	0.475
LIG_LIR_LC3C_4	368	373	PF02991	0.540
LIG_LIR_Nem_3	275	279	PF02991	0.514
LIG_LIR_Nem_3	318	323	PF02991	0.389
LIG_LYPXL_yS_3	326	329	PF13949	0.380
LIG_LYPXL_yS_3	375	378	PF13949	0.524
LIG_PCNA_yPIPBox_3	231	243	PF02747	0.510
LIG_Pex14_1	121	125	PF04695	0.516
LIG_PTB_Apo_2	119	126	PF02174	0.392
LIG_PTB_Apo_2	270	277	PF02174	0.388
LIG_PTB_Apo_2	295	302	PF02174	0.470
LIG_PTB_Phospho_1	270	276	PF10480	0.389
LIG_RPA_C_Fungi	94	106	PF08784	0.320
LIG_SH2_CRK	162	166	PF00017	0.328
LIG_SH2_PTP2	276	279	PF00017	0.492
LIG_SH2_SRC	430	433	PF00017	0.195
LIG_SH2_STAP1	245	249	PF00017	0.523
LIG_SH2_STAP1	317	321	PF00017	0.402
LIG_SH2_STAT3	158	161	PF00017	0.328
LIG_SH2_STAT3	19	22	PF00017	0.547
LIG_SH2_STAT3	245	248	PF00017	0.400
LIG_SH2_STAT5	158	161	PF00017	0.339
LIG_SH2_STAT5	276	279	PF00017	0.492
LIG_SH2_STAT5	430	433	PF00017	0.389
LIG_SH3_3	105	111	PF00018	0.362
LIG_SH3_3	321	327	PF00018	0.494
LIG_SH3_3	370	376	PF00018	0.557
LIG_SH3_3	428	434	PF00018	0.387
LIG_SH3_3	58	64	PF00018	0.398
LIG_SUMO_SIM_anti_2	275	281	PF11976	0.439
LIG_SUMO_SIM_par_1	406	413	PF11976	0.373
LIG_TRAF2_1	115	118	PF00917	0.475
LIG_TYR_ITIM	252	257	PF00017	0.510
LIG_TYR_ITIM	373	378	PF00017	0.540
LIG_UBA3_1	28	35	PF00899	0.541
LIG_UBA3_1	86	94	PF00899	0.407
MOD_CDC14_SPxK_1	212	215	PF00782	0.735
MOD_CDK_SPxK_1	209	215	PF00069	0.721
MOD_CK1_1	164	170	PF00069	0.328
MOD_CK1_1	193	199	PF00069	0.689
MOD_CK1_1	30	36	PF00069	0.500
MOD_CK1_1	341	347	PF00069	0.557
MOD_CK1_1	390	396	PF00069	0.410
MOD_CK1_1	68	74	PF00069	0.455
MOD_CK2_1	358	364	PF00069	0.349
MOD_GlcNHglycan	131	134	PF01048	0.435
MOD_GlcNHglycan	171	174	PF01048	0.328
MOD_GlcNHglycan	81	84	PF01048	0.473
MOD_GSK3_1	189	196	PF00069	0.638
MOD_GSK3_1	338	345	PF00069	0.582
MOD_GSK3_1	461	468	PF00069	0.585
MOD_LATS_1	63	69	PF00433	0.448
MOD_N-GLC_1	190	195	PF02516	0.633
MOD_N-GLC_1	341	346	PF02516	0.601
MOD_N-GLC_1	95	100	PF02516	0.435
MOD_N-GLC_2	154	156	PF02516	0.298
MOD_NEK2_1	2	7	PF00069	0.517
MOD_NEK2_1	227	232	PF00069	0.539
MOD_NEK2_1	247	252	PF00069	0.294
MOD_NEK2_1	301	306	PF00069	0.404
MOD_NEK2_1	70	75	PF00069	0.390
MOD_NEK2_1	88	93	PF00069	0.362
MOD_NEK2_1	97	102	PF00069	0.478
MOD_PIKK_1	120	126	PF00454	0.323
MOD_PIKK_1	143	149	PF00454	0.459
MOD_PIKK_1	358	364	PF00454	0.543
MOD_PIKK_1	438	444	PF00454	0.504
MOD_PKA_2	216	222	PF00069	0.621
MOD_Plk_1	193	199	PF00069	0.640
MOD_Plk_1	387	393	PF00069	0.451
MOD_Plk_4	154	160	PF00069	0.368
MOD_Plk_4	166	172	PF00069	0.280
MOD_Plk_4	247	253	PF00069	0.353
MOD_Plk_4	343	349	PF00069	0.444
MOD_Plk_4	432	438	PF00069	0.505
MOD_ProDKin_1	104	110	PF00069	0.341
MOD_ProDKin_1	209	215	PF00069	0.722
MOD_ProDKin_1	27	33	PF00069	0.519
MOD_ProDKin_1	413	419	PF00069	0.417
MOD_ProDKin_1	430	436	PF00069	0.438
MOD_ProDKin_1	71	77	PF00069	0.347
MOD_SUMO_rev_2	41	46	PF00179	0.335
TRG_ENDOCYTIC_2	162	165	PF00928	0.332
TRG_ENDOCYTIC_2	254	257	PF00928	0.407
TRG_ENDOCYTIC_2	276	279	PF00928	0.492
TRG_ENDOCYTIC_2	326	329	PF00928	0.364
TRG_ENDOCYTIC_2	375	378	PF00928	0.538
TRG_ER_diArg_1	241	243	PF00400	0.424
TRG_ER_diArg_1	282	284	PF00400	0.566
TRG_NLS_MonoCore_2	398	403	PF00514	0.518
TRG_NLS_MonoExtN_4	399	405	PF00514	0.521
TRG_Pf-PMV_PEXEL_1	235	239	PF00026	0.521
TRG_Pf-PMV_PEXEL_1	311	315	PF00026	0.510

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HUK1	Leptomonas seymouri	65%	100%
A0A0S4IV97	Bodo saltans	45%	93%
A0A1X0NN27	Trypanosomatidae	50%	100%
A0A3S7WYP3	Leishmania donovani	98%	100%
A0A422NV05	Trypanosoma rangeli	51%	100%
C9ZI50	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	97%
E9AIR3	Leishmania braziliensis	82%	100%
E9AX80	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
Q4QA96	Leishmania major	91%	100%
V5AZL8	Trypanosoma cruzi	51%	100%