Putative clathrin coat assembly protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

clathrin coat assembly protein - putative

Species:

Leishmania infantum

UniProt:

A4I0M5_LEIIN

TriTrypDb:

LINF_240005000

Length:

483

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0030135	coated vesicle	7	12
GO:0030136	clathrin-coated vesicle	8	12
GO:0031410	cytoplasmic vesicle	6	12
GO:0031982	vesicle	4	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0097708	intracellular vesicle	5	12
GO:0110165	cellular anatomical entity	1	12
GO:0000331	contractile vacuole	6	1
GO:0005737	cytoplasm	2	1
GO:0005773	vacuole	5	1
GO:0005905	clathrin-coated pit	4	1
GO:0016020	membrane	2	1
GO:0098590	plasma membrane region	3	1

Expansion

Sequence features

A4I0M5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I0M5

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	12
GO:0006897	endocytosis	5	12
GO:0006898	receptor-mediated endocytosis	6	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0016192	vesicle-mediated transport	4	12
GO:0022607	cellular component assembly	4	12
GO:0043933	protein-containing complex organization	4	12
GO:0048268	clathrin coat assembly	6	12
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0065003	protein-containing complex assembly	5	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0072583	clathrin-dependent endocytosis	7	12
GO:0006900	vesicle budding from membrane	5	1
GO:0006996	organelle organization	4	1
GO:0016050	vesicle organization	5	1
GO:0061024	membrane organization	4	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	12
GO:0005515	protein binding	2	12
GO:0005543	phospholipid binding	3	12
GO:0005545	1-phosphatidylinositol binding	5	12
GO:0008289	lipid binding	2	12
GO:0030276	clathrin binding	3	12
GO:0035091	phosphatidylinositol binding	4	12
GO:0000149	SNARE binding	3	1
GO:0005546	phosphatidylinositol-4,5-bisphosphate binding	4	1
GO:0032050	clathrin heavy chain binding	4	1
GO:0043167	ion binding	2	1
GO:0043168	anion binding	3	1
GO:1901981	phosphatidylinositol phosphate binding	5	1
GO:1902936	phosphatidylinositol bisphosphate binding	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	103	107	PF00656	0.436
CLV_C14_Caspase3-7	129	133	PF00656	0.398
CLV_NRD_NRD_1	161	163	PF00675	0.404
CLV_NRD_NRD_1	254	256	PF00675	0.381
CLV_PCSK_KEX2_1	161	163	PF00082	0.393
CLV_PCSK_KEX2_1	229	231	PF00082	0.416
CLV_PCSK_KEX2_1	246	248	PF00082	0.308
CLV_PCSK_KEX2_1	254	256	PF00082	0.378
CLV_PCSK_KEX2_1	80	82	PF00082	0.308
CLV_PCSK_PC1ET2_1	229	231	PF00082	0.416
CLV_PCSK_PC1ET2_1	246	248	PF00082	0.428
CLV_PCSK_PC1ET2_1	80	82	PF00082	0.308
CLV_PCSK_PC7_1	76	82	PF00082	0.428
CLV_PCSK_SKI1_1	116	120	PF00082	0.373
CLV_PCSK_SKI1_1	181	185	PF00082	0.385
CLV_PCSK_SKI1_1	221	225	PF00082	0.360
CLV_PCSK_SKI1_1	230	234	PF00082	0.276
CLV_PCSK_SKI1_1	275	279	PF00082	0.419
CLV_PCSK_SKI1_1	31	35	PF00082	0.428
DEG_APCC_DBOX_1	274	282	PF00400	0.377
DEG_Nend_UBRbox_3	1	3	PF02207	0.586
DEG_SPOP_SBC_1	348	352	PF00917	0.534
DOC_CDC14_PxL_1	278	286	PF14671	0.377
DOC_MAPK_DCC_7	456	464	PF00069	0.671
DOC_MAPK_gen_1	14	21	PF00069	0.452
DOC_MAPK_MEF2A_6	181	190	PF00069	0.385
DOC_MAPK_MEF2A_6	456	464	PF00069	0.671
DOC_PP1_RVXF_1	227	234	PF00149	0.345
DOC_PP2B_LxvP_1	41	44	PF13499	0.323
DOC_PP4_FxxP_1	241	244	PF00568	0.423
DOC_PP4_FxxP_1	277	280	PF00568	0.341
DOC_PP4_FxxP_1	464	467	PF00568	0.679
DOC_USP7_MATH_1	259	263	PF00917	0.461
DOC_USP7_MATH_1	390	394	PF00917	0.728
DOC_USP7_UBL2_3	31	35	PF12436	0.428
DOC_USP7_UBL2_3	47	51	PF12436	0.428
DOC_WW_Pin1_4	350	355	PF00397	0.712
DOC_WW_Pin1_4	382	387	PF00397	0.697
DOC_WW_Pin1_4	421	426	PF00397	0.678
DOC_WW_Pin1_4	450	455	PF00397	0.696
LIG_14-3-3_CanoR_1	254	263	PF00244	0.393
LIG_14-3-3_CanoR_1	308	313	PF00244	0.605
LIG_14-3-3_CanoR_1	81	90	PF00244	0.325
LIG_Actin_WH2_2	165	183	PF00022	0.385
LIG_ActinCP_TwfCPI_2	464	472	PF01115	0.640
LIG_AP2alpha_2	469	471	PF02296	0.599
LIG_BRCT_BRCA1_1	273	277	PF00533	0.393
LIG_BRCT_BRCA1_1	397	401	PF00533	0.689
LIG_EVH1_2	430	434	PF00568	0.596
LIG_EVH1_2	460	464	PF00568	0.678
LIG_FHA_1	16	22	PF00498	0.576
LIG_FHA_1	35	41	PF00498	0.455
LIG_FHA_1	370	376	PF00498	0.667
LIG_FHA_2	143	149	PF00498	0.428
LIG_FHA_2	291	297	PF00498	0.392
LIG_FHA_2	325	331	PF00498	0.689
LIG_LIR_Apic_2	239	244	PF02991	0.423
LIG_LIR_Apic_2	274	280	PF02991	0.393
LIG_LIR_Gen_1	211	217	PF02991	0.327
LIG_LIR_Gen_1	236	244	PF02991	0.341
LIG_LIR_Gen_1	258	267	PF02991	0.362
LIG_LIR_Gen_1	84	95	PF02991	0.299
LIG_LIR_Gen_1	96	104	PF02991	0.282
LIG_LIR_Nem_3	141	146	PF02991	0.369
LIG_LIR_Nem_3	167	171	PF02991	0.339
LIG_LIR_Nem_3	211	216	PF02991	0.310
LIG_LIR_Nem_3	236	241	PF02991	0.323
LIG_LIR_Nem_3	251	256	PF02991	0.323
LIG_LIR_Nem_3	258	263	PF02991	0.318
LIG_LIR_Nem_3	469	474	PF02991	0.521
LIG_LIR_Nem_3	56	60	PF02991	0.314
LIG_LIR_Nem_3	84	90	PF02991	0.299
LIG_LIR_Nem_3	96	100	PF02991	0.282
LIG_LYPXL_S_1	447	451	PF13949	0.648
LIG_LYPXL_yS_3	448	451	PF13949	0.686
LIG_MYND_1	282	286	PF01753	0.329
LIG_Pex14_1	249	253	PF04695	0.308
LIG_SH2_CRK	213	217	PF00017	0.316
LIG_SH2_NCK_1	256	260	PF00017	0.365
LIG_SH2_STAP1	256	260	PF00017	0.365
LIG_SH2_STAT5	12	15	PF00017	0.459
LIG_SH2_STAT5	138	141	PF00017	0.322
LIG_SH2_STAT5	146	149	PF00017	0.291
LIG_SH2_STAT5	171	174	PF00017	0.344
LIG_SH2_STAT5	266	269	PF00017	0.449
LIG_SH3_1	331	337	PF00018	0.690
LIG_SH3_2	467	472	PF14604	0.678
LIG_SH3_3	115	121	PF00018	0.280
LIG_SH3_3	331	337	PF00018	0.611
LIG_SH3_3	340	346	PF00018	0.616
LIG_SH3_3	351	357	PF00018	0.610
LIG_SH3_3	368	374	PF00018	0.619
LIG_SH3_3	383	389	PF00018	0.591
LIG_SH3_3	422	428	PF00018	0.679
LIG_SH3_3	448	454	PF00018	0.622
LIG_SH3_3	455	461	PF00018	0.730
LIG_SH3_3	464	470	PF00018	0.656
LIG_SUMO_SIM_par_1	87	93	PF11976	0.323
LIG_UBA3_1	223	229	PF00899	0.428
LIG_WRC_WIRS_1	21	26	PF05994	0.601
LIG_WRC_WIRS_1	94	99	PF05994	0.349
LIG_WW_3	333	337	PF00397	0.722
MOD_CDC14_SPxK_1	453	456	PF00782	0.576
MOD_CDK_SPxK_1	450	456	PF00069	0.712
MOD_CK1_1	22	28	PF00069	0.556
MOD_CK1_1	290	296	PF00069	0.392
MOD_CK1_1	3	9	PF00069	0.594
MOD_CK1_1	320	326	PF00069	0.607
MOD_CK1_1	421	427	PF00069	0.686
MOD_CK2_1	142	148	PF00069	0.339
MOD_CK2_1	22	28	PF00069	0.428
MOD_CK2_1	239	245	PF00069	0.385
MOD_CK2_1	290	296	PF00069	0.390
MOD_CK2_1	324	330	PF00069	0.709
MOD_Cter_Amidation	159	162	PF01082	0.428
MOD_GlcNHglycan	154	157	PF01048	0.469
MOD_GlcNHglycan	24	27	PF01048	0.573
MOD_GlcNHglycan	257	260	PF01048	0.391
MOD_GlcNHglycan	412	415	PF01048	0.701
MOD_GlcNHglycan	434	437	PF01048	0.735
MOD_GlcNHglycan	61	64	PF01048	0.416
MOD_GlcNHglycan	8	11	PF01048	0.591
MOD_GSK3_1	138	145	PF00069	0.412
MOD_GSK3_1	15	22	PF00069	0.571
MOD_GSK3_1	255	262	PF00069	0.354
MOD_GSK3_1	266	273	PF00069	0.262
MOD_GSK3_1	316	323	PF00069	0.560
MOD_GSK3_1	376	383	PF00069	0.687
MOD_GSK3_1	410	417	PF00069	0.696
MOD_N-GLC_1	1	6	PF02516	0.639
MOD_N-GLC_1	421	426	PF02516	0.556
MOD_N-GLC_2	206	208	PF02516	0.323
MOD_NEK2_1	108	113	PF00069	0.408
MOD_NEK2_1	152	157	PF00069	0.344
MOD_NEK2_1	271	276	PF00069	0.356
MOD_NEK2_1	34	39	PF00069	0.342
MOD_NEK2_1	474	479	PF00069	0.634
MOD_NEK2_1	53	58	PF00069	0.224
MOD_NEK2_2	259	264	PF00069	0.428
MOD_PIKK_1	138	144	PF00454	0.369
MOD_PKA_2	299	305	PF00069	0.451
MOD_Plk_1	239	245	PF00069	0.349
MOD_Plk_1	320	326	PF00069	0.678
MOD_Plk_2-3	239	245	PF00069	0.416
MOD_Plk_4	142	148	PF00069	0.334
MOD_Plk_4	266	272	PF00069	0.346
MOD_Plk_4	287	293	PF00069	0.324
MOD_Plk_4	90	96	PF00069	0.323
MOD_ProDKin_1	350	356	PF00069	0.709
MOD_ProDKin_1	382	388	PF00069	0.694
MOD_ProDKin_1	421	427	PF00069	0.675
MOD_ProDKin_1	450	456	PF00069	0.694
MOD_SUMO_rev_2	3	9	PF00179	0.539
MOD_SUMO_rev_2	323	333	PF00179	0.666
TRG_DiLeu_BaEn_1	148	153	PF01217	0.241
TRG_DiLeu_BaEn_1	211	216	PF01217	0.428
TRG_ENDOCYTIC_2	115	118	PF00928	0.428
TRG_ENDOCYTIC_2	213	216	PF00928	0.316
TRG_ENDOCYTIC_2	448	451	PF00928	0.686
TRG_ER_diArg_1	161	163	PF00400	0.427
TRG_ER_diArg_1	253	255	PF00400	0.349
TRG_Pf-PMV_PEXEL_1	150	154	PF00026	0.289
TRG_Pf-PMV_PEXEL_1	285	289	PF00026	0.394

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1II26	Leptomonas seymouri	58%	100%
A0A0S4J0L1	Bodo saltans	38%	100%
A0A1X0NIY0	Trypanosomatidae	42%	97%
A0A3S5H7C6	Leishmania donovani	98%	100%
A0A422P449	Trypanosoma rangeli	38%	96%
A4HD60	Leishmania braziliensis	72%	99%
D0A6X3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	98%
E9AWJ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
P94017	Arabidopsis thaliana	23%	70%
Q4QAX8	Leishmania major	88%	100%
Q8LBH2	Arabidopsis thaliana	23%	85%
Q8VYT2	Arabidopsis thaliana	21%	80%
Q9LHS0	Arabidopsis thaliana	24%	89%
Q9LVD8	Arabidopsis thaliana	24%	82%
V5DSQ8	Trypanosoma cruzi	44%	97%