Phosphoinositide phospholipase C

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

phosphoinositide-specific phospholipase C - putative

Species:

Leishmania infantum

UniProt:

A4I027_LEIIN

TriTrypDb:

LINF_220023100

Length:

784

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A4I027

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I027

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	7
GO:0007165	signal transduction	2	7
GO:0008152	metabolic process	1	7
GO:0009056	catabolic process	2	7
GO:0009987	cellular process	1	7
GO:0016042	lipid catabolic process	4	7
GO:0035556	intracellular signal transduction	3	7
GO:0044238	primary metabolic process	2	7
GO:0050789	regulation of biological process	2	7
GO:0050794	regulation of cellular process	3	7
GO:0065007	biological regulation	1	7
GO:0071704	organic substance metabolic process	2	7
GO:1901575	organic substance catabolic process	3	7
GO:0048015	phosphatidylinositol-mediated signaling	5	1
GO:0048017	inositol lipid-mediated signaling	4	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	7
GO:0004435	phosphatidylinositol phospholipase C activity	7	7
GO:0004620	phospholipase activity	5	7
GO:0004629	phospholipase C activity	6	7
GO:0005488	binding	1	5
GO:0005509	calcium ion binding	5	5
GO:0008081	phosphoric diester hydrolase activity	5	7
GO:0016298	lipase activity	4	7
GO:0016787	hydrolase activity	2	7
GO:0016788	hydrolase activity, acting on ester bonds	3	7
GO:0042578	phosphoric ester hydrolase activity	4	7
GO:0043167	ion binding	2	5
GO:0043169	cation binding	3	5
GO:0046872	metal ion binding	4	5

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	484	488	PF00656	0.570
CLV_C14_Caspase3-7	581	585	PF00656	0.333
CLV_C14_Caspase3-7	757	761	PF00656	0.378
CLV_NRD_NRD_1	504	506	PF00675	0.601
CLV_NRD_NRD_1	551	553	PF00675	0.333
CLV_NRD_NRD_1	753	755	PF00675	0.377
CLV_PCSK_KEX2_1	144	146	PF00082	0.433
CLV_PCSK_KEX2_1	195	197	PF00082	0.438
CLV_PCSK_KEX2_1	466	468	PF00082	0.456
CLV_PCSK_KEX2_1	86	88	PF00082	0.588
CLV_PCSK_PC1ET2_1	144	146	PF00082	0.433
CLV_PCSK_PC1ET2_1	195	197	PF00082	0.438
CLV_PCSK_PC1ET2_1	466	468	PF00082	0.456
CLV_PCSK_PC1ET2_1	86	88	PF00082	0.588
CLV_PCSK_SKI1_1	121	125	PF00082	0.451
CLV_PCSK_SKI1_1	205	209	PF00082	0.370
CLV_PCSK_SKI1_1	210	214	PF00082	0.367
CLV_PCSK_SKI1_1	280	284	PF00082	0.540
CLV_PCSK_SKI1_1	313	317	PF00082	0.440
CLV_PCSK_SKI1_1	354	358	PF00082	0.313
CLV_PCSK_SKI1_1	387	391	PF00082	0.396
CLV_PCSK_SKI1_1	42	46	PF00082	0.519
CLV_PCSK_SKI1_1	617	621	PF00082	0.346
CLV_PCSK_SKI1_1	636	640	PF00082	0.322
CLV_PCSK_SKI1_1	650	654	PF00082	0.281
CLV_PCSK_SKI1_1	87	91	PF00082	0.558
CLV_PCSK_SKI1_1	96	100	PF00082	0.419
DEG_APCC_DBOX_1	120	128	PF00400	0.452
DEG_APCC_DBOX_1	716	724	PF00400	0.298
DEG_Nend_UBRbox_1	1	4	PF02207	0.564
DEG_SCF_FBW7_1	684	691	PF00400	0.438
DEG_SPOP_SBC_1	35	39	PF00917	0.515
DOC_CYCLIN_RxL_1	352	359	PF00134	0.313
DOC_MAPK_gen_1	195	203	PF00069	0.347
DOC_MAPK_gen_1	505	514	PF00069	0.651
DOC_MAPK_MEF2A_6	636	645	PF00069	0.520
DOC_MAPK_MEF2A_6	717	724	PF00069	0.298
DOC_PP1_RVXF_1	385	391	PF00149	0.313
DOC_PP4_FxxP_1	438	441	PF00568	0.278
DOC_PP4_FxxP_1	660	663	PF00568	0.322
DOC_USP7_MATH_1	123	127	PF00917	0.519
DOC_USP7_MATH_1	441	445	PF00917	0.333
DOC_USP7_MATH_1	52	56	PF00917	0.483
DOC_USP7_MATH_1	520	524	PF00917	0.403
DOC_USP7_MATH_1	578	582	PF00917	0.403
DOC_USP7_MATH_1	663	667	PF00917	0.339
DOC_USP7_MATH_1	68	72	PF00917	0.423
DOC_USP7_MATH_2	108	114	PF00917	0.535
DOC_USP7_UBL2_3	160	164	PF12436	0.526
DOC_USP7_UBL2_3	460	464	PF12436	0.322
DOC_USP7_UBL2_3	751	755	PF12436	0.432
DOC_WW_Pin1_4	3	8	PF00397	0.539
DOC_WW_Pin1_4	300	305	PF00397	0.549
DOC_WW_Pin1_4	450	455	PF00397	0.379
DOC_WW_Pin1_4	586	591	PF00397	0.298
DOC_WW_Pin1_4	684	689	PF00397	0.412
LIG_14-3-3_CanoR_1	145	153	PF00244	0.439
LIG_14-3-3_CanoR_1	51	57	PF00244	0.486
LIG_14-3-3_CanoR_1	513	521	PF00244	0.455
LIG_14-3-3_CanoR_1	636	641	PF00244	0.475
LIG_14-3-3_CanoR_1	650	655	PF00244	0.291
LIG_14-3-3_CanoR_1	717	723	PF00244	0.396
LIG_14-3-3_CanoR_1	732	739	PF00244	0.201
LIG_14-3-3_CanoR_1	754	759	PF00244	0.350
LIG_14-3-3_CanoR_1	87	92	PF00244	0.518
LIG_14-3-3_CanoR_1	96	103	PF00244	0.488
LIG_Actin_RPEL_3	725	744	PF02755	0.298
LIG_AP2alpha_2	672	674	PF02296	0.396
LIG_APCC_ABBA_1	212	217	PF00400	0.424
LIG_APCC_ABBA_1	302	307	PF00400	0.559
LIG_APCC_ABBA_1	675	680	PF00400	0.396
LIG_BRCT_BRCA1_1	112	116	PF00533	0.519
LIG_BRCT_BRCA1_1	302	306	PF00533	0.565
LIG_BRCT_BRCA1_1	434	438	PF00533	0.389
LIG_BRCT_BRCA1_1	573	577	PF00533	0.341
LIG_BRCT_BRCA1_1	607	611	PF00533	0.333
LIG_Clathr_ClatBox_1	521	525	PF01394	0.298
LIG_deltaCOP1_diTrp_1	270	276	PF00928	0.386
LIG_deltaCOP1_diTrp_1	710	718	PF00928	0.313
LIG_EH1_1	319	327	PF00400	0.298
LIG_FHA_1	160	166	PF00498	0.583
LIG_FHA_1	206	212	PF00498	0.393
LIG_FHA_1	28	34	PF00498	0.438
LIG_FHA_1	287	293	PF00498	0.431
LIG_FHA_1	383	389	PF00498	0.307
LIG_FHA_1	484	490	PF00498	0.694
LIG_FHA_1	651	657	PF00498	0.360
LIG_FHA_1	674	680	PF00498	0.430
LIG_FHA_1	689	695	PF00498	0.231
LIG_FHA_1	707	713	PF00498	0.269
LIG_FHA_2	415	421	PF00498	0.343
LIG_FHA_2	482	488	PF00498	0.581
LIG_FHA_2	55	61	PF00498	0.472
LIG_FHA_2	637	643	PF00498	0.511
LIG_Integrin_RGD_1	467	469	PF01839	0.602
LIG_LIR_Apic_2	367	372	PF02991	0.396
LIG_LIR_Apic_2	435	441	PF02991	0.278
LIG_LIR_Apic_2	55	59	PF02991	0.473
LIG_LIR_Apic_2	658	663	PF02991	0.313
LIG_LIR_Apic_2	761	767	PF02991	0.524
LIG_LIR_Gen_1	188	198	PF02991	0.473
LIG_LIR_Gen_1	222	232	PF02991	0.338
LIG_LIR_Gen_1	258	267	PF02991	0.470
LIG_LIR_Gen_1	316	326	PF02991	0.430
LIG_LIR_Gen_1	496	502	PF02991	0.537
LIG_LIR_Gen_1	596	603	PF02991	0.298
LIG_LIR_Gen_1	672	682	PF02991	0.395
LIG_LIR_Nem_3	222	228	PF02991	0.322
LIG_LIR_Nem_3	237	241	PF02991	0.296
LIG_LIR_Nem_3	258	263	PF02991	0.414
LIG_LIR_Nem_3	294	300	PF02991	0.398
LIG_LIR_Nem_3	405	410	PF02991	0.322
LIG_LIR_Nem_3	585	591	PF02991	0.333
LIG_LIR_Nem_3	596	601	PF02991	0.217
LIG_LIR_Nem_3	647	652	PF02991	0.372
LIG_LIR_Nem_3	672	677	PF02991	0.356
LIG_LYPXL_yS_3	407	410	PF13949	0.313
LIG_MLH1_MIPbox_1	302	306	PF16413	0.425
LIG_NRBOX	432	438	PF00104	0.403
LIG_PDZ_Class_3	779	784	PF00595	0.499
LIG_Pex14_1	272	276	PF04695	0.386
LIG_Pex14_1	594	598	PF04695	0.298
LIG_Pex14_2	16	20	PF04695	0.497
LIG_Pex14_2	438	442	PF04695	0.322
LIG_Pex14_2	674	678	PF04695	0.396
LIG_Pex14_2	699	703	PF04695	0.313
LIG_PTB_Apo_2	693	700	PF02174	0.313
LIG_Rb_pABgroove_1	175	183	PF01858	0.298
LIG_SH2_CRK	588	592	PF00017	0.333
LIG_SH2_CRK	764	768	PF00017	0.523
LIG_SH2_GRB2like	232	235	PF00017	0.465
LIG_SH2_NCK_1	56	60	PF00017	0.473
LIG_SH2_NCK_1	764	768	PF00017	0.531
LIG_SH2_SRC	91	94	PF00017	0.444
LIG_SH2_STAP1	232	236	PF00017	0.474
LIG_SH2_STAP1	400	404	PF00017	0.313
LIG_SH2_STAT3	243	246	PF00017	0.419
LIG_SH2_STAT5	102	105	PF00017	0.525
LIG_SH2_STAT5	243	246	PF00017	0.420
LIG_SH2_STAT5	300	303	PF00017	0.453
LIG_SH2_STAT5	305	308	PF00017	0.453
LIG_SH2_STAT5	329	332	PF00017	0.315
LIG_SH2_STAT5	338	341	PF00017	0.322
LIG_SH2_STAT5	382	385	PF00017	0.322
LIG_SH2_STAT5	497	500	PF00017	0.615
LIG_SH2_STAT5	537	540	PF00017	0.322
LIG_SH2_STAT5	56	59	PF00017	0.473
LIG_SH2_STAT5	628	631	PF00017	0.322
LIG_SH2_STAT5	777	780	PF00017	0.529
LIG_SH2_STAT5	91	94	PF00017	0.467
LIG_SH3_3	23	29	PF00018	0.483
LIG_SH3_3	298	304	PF00018	0.366
LIG_SH3_3	635	641	PF00018	0.413
LIG_SH3_3	64	70	PF00018	0.405
LIG_SH3_3	667	673	PF00018	0.423
LIG_SUMO_SIM_anti_2	279	285	PF11976	0.565
LIG_SUMO_SIM_anti_2	653	658	PF11976	0.356
LIG_SUMO_SIM_anti_2	719	724	PF11976	0.298
LIG_SUMO_SIM_par_1	29	34	PF11976	0.509
LIG_SUMO_SIM_par_1	407	412	PF11976	0.325
LIG_SUMO_SIM_par_1	519	527	PF11976	0.322
LIG_SUMO_SIM_par_1	651	658	PF11976	0.314
LIG_SxIP_EBH_1	86	98	PF03271	0.398
LIG_TRAF2_1	131	134	PF00917	0.582
LIG_TRAF2_1	389	392	PF00917	0.333
LIG_TRFH_1	628	632	PF08558	0.322
LIG_UBA3_1	743	751	PF00899	0.335
LIG_WRC_WIRS_1	260	265	PF05994	0.423
MOD_CDK_SPK_2	684	689	PF00069	0.298
MOD_CK1_1	146	152	PF00069	0.440
MOD_CK1_1	256	262	PF00069	0.548
MOD_CK1_1	334	340	PF00069	0.322
MOD_CK1_1	346	352	PF00069	0.284
MOD_CK1_1	37	43	PF00069	0.514
MOD_CK1_1	488	494	PF00069	0.608
MOD_CK1_1	523	529	PF00069	0.295
MOD_CK1_1	547	553	PF00069	0.356
MOD_CK1_1	586	592	PF00069	0.298
MOD_CK1_1	607	613	PF00069	0.356
MOD_CK1_1	658	664	PF00069	0.372
MOD_CK1_1	690	696	PF00069	0.396
MOD_CK2_1	128	134	PF00069	0.575
MOD_CK2_1	187	193	PF00069	0.372
MOD_CK2_1	234	240	PF00069	0.514
MOD_CK2_1	414	420	PF00069	0.356
MOD_CK2_1	54	60	PF00069	0.474
MOD_CK2_1	545	551	PF00069	0.392
MOD_CK2_1	663	669	PF00069	0.298
MOD_CK2_1	79	85	PF00069	0.452
MOD_GlcNHglycan	11	14	PF01048	0.434
MOD_GlcNHglycan	112	115	PF01048	0.527
MOD_GlcNHglycan	149	152	PF01048	0.454
MOD_GlcNHglycan	184	188	PF01048	0.325
MOD_GlcNHglycan	307	310	PF01048	0.446
MOD_GlcNHglycan	404	407	PF01048	0.439
MOD_GlcNHglycan	514	517	PF01048	0.481
MOD_GlcNHglycan	518	521	PF01048	0.447
MOD_GlcNHglycan	595	598	PF01048	0.333
MOD_GlcNHglycan	657	660	PF01048	0.320
MOD_GlcNHglycan	663	666	PF01048	0.326
MOD_GlcNHglycan	760	763	PF01048	0.440
MOD_GSK3_1	139	146	PF00069	0.481
MOD_GSK3_1	155	162	PF00069	0.462
MOD_GSK3_1	183	190	PF00069	0.403
MOD_GSK3_1	201	208	PF00069	0.356
MOD_GSK3_1	219	226	PF00069	0.123
MOD_GSK3_1	230	237	PF00069	0.433
MOD_GSK3_1	286	293	PF00069	0.464
MOD_GSK3_1	3	10	PF00069	0.533
MOD_GSK3_1	330	337	PF00069	0.322
MOD_GSK3_1	342	349	PF00069	0.322
MOD_GSK3_1	446	453	PF00069	0.356
MOD_GSK3_1	481	488	PF00069	0.651
MOD_GSK3_1	512	519	PF00069	0.424
MOD_GSK3_1	520	527	PF00069	0.322
MOD_GSK3_1	578	585	PF00069	0.356
MOD_GSK3_1	661	668	PF00069	0.439
MOD_GSK3_1	683	690	PF00069	0.386
MOD_GSK3_1	754	761	PF00069	0.362
MOD_LATS_1	634	640	PF00433	0.367
MOD_LATS_1	730	736	PF00433	0.298
MOD_N-GLC_1	110	115	PF02516	0.541
MOD_N-GLC_1	160	165	PF02516	0.519
MOD_N-GLC_1	568	573	PF02516	0.403
MOD_N-GLC_1	586	591	PF02516	0.403
MOD_N-GLC_1	758	763	PF02516	0.370
MOD_N-GLC_2	616	618	PF02516	0.333
MOD_N-GLC_2	623	625	PF02516	0.259
MOD_N-GLC_2	731	733	PF02516	0.298
MOD_NEK2_1	116	121	PF00069	0.390
MOD_NEK2_1	139	144	PF00069	0.396
MOD_NEK2_1	20	25	PF00069	0.486
MOD_NEK2_1	330	335	PF00069	0.283
MOD_NEK2_1	402	407	PF00069	0.356
MOD_NEK2_1	500	505	PF00069	0.449
MOD_NEK2_1	758	763	PF00069	0.395
MOD_NEK2_1	77	82	PF00069	0.543
MOD_NEK2_2	393	398	PF00069	0.356
MOD_NEK2_2	673	678	PF00069	0.396
MOD_NEK2_2	711	716	PF00069	0.298
MOD_PIKK_1	102	108	PF00454	0.300
MOD_PIKK_1	347	353	PF00454	0.298
MOD_PIKK_1	733	739	PF00454	0.298
MOD_PKA_1	313	319	PF00069	0.453
MOD_PKA_1	754	760	PF00069	0.355
MOD_PKA_2	219	225	PF00069	0.448
MOD_PKA_2	256	262	PF00069	0.488
MOD_PKA_2	27	33	PF00069	0.502
MOD_PKA_2	383	389	PF00069	0.283
MOD_PKA_2	512	518	PF00069	0.479
MOD_PKA_2	52	58	PF00069	0.479
MOD_PKA_2	716	722	PF00069	0.446
MOD_PKA_2	731	737	PF00069	0.201
MOD_Plk_1	110	116	PF00069	0.533
MOD_Plk_1	187	193	PF00069	0.403
MOD_Plk_1	533	539	PF00069	0.322
MOD_Plk_1	568	574	PF00069	0.403
MOD_Plk_2-3	481	487	PF00069	0.592
MOD_Plk_4	259	265	PF00069	0.425
MOD_Plk_4	334	340	PF00069	0.343
MOD_Plk_4	432	438	PF00069	0.452
MOD_Plk_4	533	539	PF00069	0.322
MOD_Plk_4	673	679	PF00069	0.362
MOD_Plk_4	718	724	PF00069	0.316
MOD_Plk_4	766	772	PF00069	0.418
MOD_Plk_4	87	93	PF00069	0.426
MOD_ProDKin_1	3	9	PF00069	0.536
MOD_ProDKin_1	300	306	PF00069	0.550
MOD_ProDKin_1	450	456	PF00069	0.379
MOD_ProDKin_1	586	592	PF00069	0.298
MOD_ProDKin_1	684	690	PF00069	0.412
MOD_SUMO_rev_2	188	197	PF00179	0.396
MOD_SUMO_rev_2	525	535	PF00179	0.397
TRG_DiLeu_BaEn_1	193	198	PF01217	0.403
TRG_DiLeu_BaEn_1	279	284	PF01217	0.558
TRG_DiLeu_BaEn_3	133	139	PF01217	0.435
TRG_DiLeu_BaLyEn_6	587	592	PF01217	0.333
TRG_ENDOCYTIC_2	191	194	PF00928	0.403
TRG_ENDOCYTIC_2	319	322	PF00928	0.361
TRG_ENDOCYTIC_2	407	410	PF00928	0.333
TRG_ENDOCYTIC_2	497	500	PF00928	0.545
TRG_ENDOCYTIC_2	588	591	PF00928	0.333
TRG_ER_diArg_1	1	4	PF00400	0.564
TRG_ER_diArg_1	50	53	PF00400	0.503
TRG_NLS_MonoCore_2	310	315	PF00514	0.276
TRG_NLS_MonoExtC_3	311	317	PF00514	0.283
TRG_NLS_MonoExtN_4	311	316	PF00514	0.284
TRG_Pf-PMV_PEXEL_1	195	199	PF00026	0.385
TRG_Pf-PMV_PEXEL_1	205	209	PF00026	0.385
TRG_Pf-PMV_PEXEL_1	650	655	PF00026	0.322
TRG_Pf-PMV_PEXEL_1	96	100	PF00026	0.500

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HWX7	Leptomonas seymouri	66%	100%
A0A0N1I1N0	Leptomonas seymouri	33%	100%
A0A0N1IKD7	Leptomonas seymouri	29%	100%
A0A0S4ITT3	Bodo saltans	28%	90%
A0A0S4JUS2	Bodo saltans	30%	100%
A0A1X0P2K1	Trypanosomatidae	31%	100%
A0A3Q8IF05	Leishmania donovani	34%	100%
A0A3S7X8U7	Leishmania donovani	31%	100%
A0A422N571	Trypanosoma rangeli	46%	100%
A4HCK9	Leishmania braziliensis	72%	100%
A4HI17	Leishmania braziliensis	24%	100%
A4HM43	Leishmania braziliensis	33%	100%
A4I5X4	Leishmania infantum	34%	100%
A4IAQ5	Leishmania infantum	30%	100%
A5D6R3	Danio rerio	34%	100%
D0A7A3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E9AEI1	Leishmania major	32%	100%
E9B168	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9B5P2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
O13433	Candida albicans	28%	71%
O89040	Rattus norvegicus	34%	66%
P10688	Rattus norvegicus	33%	100%
P10895	Bos taurus	34%	100%
P21671	Bos taurus	33%	99%
P32383	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	90%
P40977	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	87%
P51178	Homo sapiens	33%	100%
Q00722	Homo sapiens	32%	66%
Q02158	Dictyostelium discoideum	32%	98%
Q15147	Homo sapiens	32%	67%
Q1RML2	Bos taurus	29%	100%
Q2VRL0	Gallus gallus	32%	100%
Q32NH8	Xenopus laevis	32%	100%
Q39032	Arabidopsis thaliana	30%	100%
Q39033	Arabidopsis thaliana	28%	100%
Q4Q6Z7	Leishmania major	33%	100%
Q4QBH9	Leishmania major	85%	100%
Q56W08	Arabidopsis thaliana	29%	100%
Q5FX52	Rattus norvegicus	30%	100%
Q5RET0	Pongo abelii	33%	100%
Q62711	Rattus norvegicus	32%	100%
Q86YW0	Homo sapiens	30%	100%
Q8K2J0	Mus musculus	33%	100%
Q8K394	Mus musculus	30%	70%
Q8K3R3	Mus musculus	32%	97%
Q8K4D7	Mus musculus	30%	100%
Q8N3E9	Homo sapiens	33%	99%
Q8R3B1	Mus musculus	32%	100%
Q8SPR7	Sus scrofa	33%	100%
Q944C1	Arabidopsis thaliana	28%	100%
Q944C2	Arabidopsis thaliana	27%	100%
Q95JS1	Macaca fascicularis	29%	100%
Q9BRC7	Homo sapiens	33%	100%
Q9LY51	Arabidopsis thaliana	27%	100%
Q9QW07	Rattus norvegicus	32%	67%
Q9UPR0	Homo sapiens	30%	70%