Poly(A)-specific ribonuclease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

CCR4 associated factor - putative

Species:

Leishmania infantum

UniProt:

A4I022_LEIIN

TriTrypDb:

LINF_220022600

Length:

338

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	11
GO:0030014	CCR4-NOT complex	3	11
GO:0032991	protein-containing complex	1	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	11
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	11
GO:0110165	cellular anatomical entity	1	12
GO:0140535	intracellular protein-containing complex	2	12
GO:0000932	P-body	5	1
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0010494	cytoplasmic stress granule	5	1
GO:0030015	CCR4-NOT core complex	3	1
GO:0035770	ribonucleoprotein granule	3	1
GO:0036464	cytoplasmic ribonucleoprotein granule	4	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0099080	supramolecular complex	2	1

Expansion

Sequence features

A4I022

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4I022

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006401	RNA catabolic process	5	12
GO:0006402	mRNA catabolic process	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009056	catabolic process	2	12
GO:0009057	macromolecule catabolic process	4	12
GO:0009892	negative regulation of metabolic process	4	12
GO:0009987	cellular process	1	12
GO:0010468	regulation of gene expression	5	12
GO:0010605	negative regulation of macromolecule metabolic process	5	12
GO:0010629	negative regulation of gene expression	6	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0019222	regulation of metabolic process	3	12
GO:0019439	aromatic compound catabolic process	4	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034655	nucleobase-containing compound catabolic process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044248	cellular catabolic process	3	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0044265	obsolete cellular macromolecule catabolic process	4	12
GO:0044270	cellular nitrogen compound catabolic process	4	12
GO:0046483	heterocycle metabolic process	3	12
GO:0046700	heterocycle catabolic process	4	12
GO:0048519	negative regulation of biological process	3	12
GO:0050789	regulation of biological process	2	12
GO:0060255	regulation of macromolecule metabolic process	4	12
GO:0065007	biological regulation	1	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901361	organic cyclic compound catabolic process	4	12
GO:1901575	organic substance catabolic process	3	12
GO:0000291	nuclear-transcribed mRNA catabolic process, exonucleolytic	8	1
GO:0000956	nuclear-transcribed mRNA catabolic process	7	1
GO:0043928	exonucleolytic catabolism of deadenylated mRNA	9	1

Molecular functions
Term	Name	Level	Count
GO:0000175	3'-5'-RNA exonuclease activity	7	12
GO:0003676	nucleic acid binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004518	nuclease activity	4	12
GO:0004527	exonuclease activity	5	12
GO:0004532	RNA exonuclease activity	5	12
GO:0004535	poly(A)-specific ribonuclease activity	8	12
GO:0004540	RNA nuclease activity	4	12
GO:0005488	binding	1	12
GO:0008408	3'-5' exonuclease activity	6	12
GO:0016787	hydrolase activity	2	12
GO:0016788	hydrolase activity, acting on ester bonds	3	12
GO:0016796	exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	12
GO:0016896	RNA exonuclease activity, producing 5'-phosphomonoesters	6	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003723	RNA binding	4	1
GO:0003727	single-stranded RNA binding	5	1
GO:0008143	poly(A) binding	7	1
GO:0070717	poly-purine tract binding	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	242	244	PF00675	0.244
CLV_NRD_NRD_1	336	338	PF00675	0.732
CLV_PCSK_KEX2_1	242	244	PF00082	0.244
CLV_PCSK_KEX2_1	336	338	PF00082	0.732
CLV_PCSK_SKI1_1	218	222	PF00082	0.241
CLV_Separin_Metazoa	136	140	PF03568	0.530
DOC_MAPK_MEF2A_6	54	61	PF00069	0.462
DOC_MAPK_MEF2A_6	95	103	PF00069	0.444
DOC_PP1_RVXF_1	172	179	PF00149	0.297
DOC_PP4_FxxP_1	310	313	PF00568	0.689
DOC_SPAK_OSR1_1	122	126	PF12202	0.549
DOC_USP7_MATH_1	131	135	PF00917	0.444
DOC_USP7_MATH_1	288	292	PF00917	0.556
DOC_USP7_MATH_1	330	334	PF00917	0.683
DOC_USP7_MATH_1	57	61	PF00917	0.464
DOC_USP7_MATH_1	72	76	PF00917	0.486
DOC_WW_Pin1_4	13	18	PF00397	0.604
DOC_WW_Pin1_4	290	295	PF00397	0.510
DOC_WW_Pin1_4	3	8	PF00397	0.693
DOC_WW_Pin1_4	309	314	PF00397	0.399
LIG_14-3-3_CanoR_1	222	230	PF00244	0.456
LIG_14-3-3_CterR_2	336	338	PF00244	0.596
LIG_BRCT_BRCA1_1	226	230	PF00533	0.530
LIG_BRCT_BRCA1_1	306	310	PF00533	0.700
LIG_BRCT_BRCA1_1	322	326	PF00533	0.390
LIG_eIF4E_1	182	188	PF01652	0.456
LIG_FHA_1	270	276	PF00498	0.511
LIG_FHA_2	76	82	PF00498	0.505
LIG_IRF3_LxIS_1	158	164	PF10401	0.337
LIG_LIR_Apic_2	307	313	PF02991	0.687
LIG_LIR_Gen_1	146	155	PF02991	0.474
LIG_LIR_Gen_1	156	163	PF02991	0.327
LIG_LIR_Gen_1	43	53	PF02991	0.453
LIG_LIR_Gen_1	55	66	PF02991	0.419
LIG_LIR_Gen_1	81	91	PF02991	0.444
LIG_LIR_Nem_3	146	151	PF02991	0.451
LIG_LIR_Nem_3	156	161	PF02991	0.353
LIG_LIR_Nem_3	183	187	PF02991	0.481
LIG_LIR_Nem_3	43	49	PF02991	0.439
LIG_LIR_Nem_3	55	61	PF02991	0.420
LIG_LIR_Nem_3	62	68	PF02991	0.439
LIG_LIR_Nem_3	81	87	PF02991	0.444
LIG_LYPXL_yS_3	56	59	PF13949	0.436
LIG_MAD2	189	197	PF02301	0.456
LIG_NRBOX	216	222	PF00104	0.530
LIG_Pex14_1	117	121	PF04695	0.444
LIG_Pex14_2	119	123	PF04695	0.444
LIG_Pex14_2	211	215	PF04695	0.475
LIG_SH2_CRK	84	88	PF00017	0.444
LIG_SH2_GRB2like	147	150	PF00017	0.530
LIG_SH2_STAP1	10	14	PF00017	0.733
LIG_SH2_STAT5	10	13	PF00017	0.659
LIG_SH2_STAT5	147	150	PF00017	0.444
LIG_SH2_STAT5	186	189	PF00017	0.444
LIG_SH2_STAT5	219	222	PF00017	0.444
LIG_SH2_STAT5	234	237	PF00017	0.444
LIG_SH2_STAT5	261	264	PF00017	0.338
LIG_SH3_3	324	330	PF00018	0.587
LIG_SH3_3	66	72	PF00018	0.442
LIG_SUMO_SIM_anti_2	133	139	PF11976	0.505
LIG_UBA3_1	210	218	PF00899	0.530
LIG_WRC_WIRS_1	73	78	PF05994	0.505
MOD_CK1_1	254	260	PF00069	0.316
MOD_CK1_1	290	296	PF00069	0.524
MOD_CK1_1	3	9	PF00069	0.692
MOD_CK1_1	322	328	PF00069	0.646
MOD_CK1_1	60	66	PF00069	0.466
MOD_CK1_1	75	81	PF00069	0.465
MOD_CK2_1	121	127	PF00069	0.505
MOD_CK2_1	75	81	PF00069	0.485
MOD_GlcNHglycan	164	167	PF01048	0.334
MOD_GlcNHglycan	295	298	PF01048	0.572
MOD_GlcNHglycan	30	33	PF01048	0.567
MOD_GlcNHglycan	322	325	PF01048	0.634
MOD_GSK3_1	20	27	PF00069	0.512
MOD_GSK3_1	265	272	PF00069	0.495
MOD_GSK3_1	288	295	PF00069	0.550
MOD_GSK3_1	298	305	PF00069	0.602
MOD_GSK3_1	72	79	PF00069	0.496
MOD_N-GLC_1	308	313	PF02516	0.647
MOD_NEK2_1	121	126	PF00069	0.505
MOD_NEK2_1	161	166	PF00069	0.336
MOD_NEK2_1	203	208	PF00069	0.505
MOD_NEK2_1	235	240	PF00069	0.453
MOD_NEK2_1	265	270	PF00069	0.486
MOD_NEK2_1	28	33	PF00069	0.448
MOD_NEK2_1	99	104	PF00069	0.442
MOD_NEK2_2	72	77	PF00069	0.472
MOD_NEK2_2	83	88	PF00069	0.408
MOD_PIKK_1	298	304	PF00454	0.664
MOD_PKA_2	121	127	PF00069	0.505
MOD_PKA_2	221	227	PF00069	0.444
MOD_PKA_2	265	271	PF00069	0.457
MOD_Plk_1	153	159	PF00069	0.483
MOD_Plk_4	210	216	PF00069	0.463
MOD_Plk_4	251	257	PF00069	0.317
MOD_ProDKin_1	13	19	PF00069	0.602
MOD_ProDKin_1	290	296	PF00069	0.507
MOD_ProDKin_1	3	9	PF00069	0.694
MOD_ProDKin_1	309	315	PF00069	0.396
MOD_SUMO_rev_2	213	220	PF00179	0.505
TRG_ENDOCYTIC_2	261	264	PF00928	0.338
TRG_ENDOCYTIC_2	56	59	PF00928	0.437
TRG_ENDOCYTIC_2	84	87	PF00928	0.444
TRG_ER_diArg_1	241	243	PF00400	0.450
TRG_ER_diArg_1	335	337	PF00400	0.704
TRG_Pf-PMV_PEXEL_1	194	198	PF00026	0.256
TRG_Pf-PMV_PEXEL_1	50	55	PF00026	0.256

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4Q3	Leptomonas seymouri	84%	100%
A0A0S4J507	Bodo saltans	72%	98%
A0A1X0NTJ0	Trypanosomatidae	72%	100%
A0A3Q8IBK8	Leishmania donovani	100%	100%
A0A422N5D9	Trypanosoma rangeli	71%	100%
A4HCK3	Leishmania braziliensis	94%	100%
A4II96	Xenopus tropicalis	52%	100%
C9ZQ67	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	73%	96%
E9AVZ5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
O64773	Arabidopsis thaliana	33%	100%
O74856	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	48%	100%
P39008	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	38%	78%
Q08BM8	Danio rerio	51%	100%
Q17345	Caenorhabditis elegans	46%	100%
Q3KQ85	Xenopus laevis	51%	100%
Q3ZC01	Bos taurus	52%	100%
Q4QBI4	Leishmania major	99%	100%
Q5ZJV9	Gallus gallus	52%	100%
Q60809	Mus musculus	52%	100%
Q9C6M9	Arabidopsis thaliana	32%	100%
Q9D8X5	Mus musculus	49%	100%
Q9FMS6	Arabidopsis thaliana	43%	100%
Q9LEU4	Arabidopsis thaliana	47%	100%
Q9LXM2	Arabidopsis thaliana	42%	100%
Q9S9P2	Arabidopsis thaliana	43%	100%
Q9SAI2	Arabidopsis thaliana	48%	100%
Q9SFX6	Arabidopsis thaliana	33%	100%
Q9SHJ0	Arabidopsis thaliana	32%	94%
Q9SKZ2	Arabidopsis thaliana	51%	100%
Q9UFF9	Homo sapiens	49%	100%
Q9UIV1	Homo sapiens	52%	100%
V5B7G1	Trypanosoma cruzi	74%	100%