Nucleosome assembly protein

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Nucleosome assembly protein (NAP) - putative

Species:

Leishmania infantum

UniProt:

A4HYX2_LEIIN

TriTrypDb:

LINF_200018100

Length:

280

Annotations

Annotations by Jardim et al.

Histone/chromosome structure, nucleosome assembly (NAP), putative

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 5

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0000785	chromatin	2	1
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A4HYX2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HYX2

Function

Biological processes
Term	Name	Level	Count
GO:0006325	chromatin organization	4	12
GO:0006334	nucleosome assembly	7	12
GO:0006338	chromatin remodeling	5	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0022607	cellular component assembly	4	12
GO:0034728	nucleosome organization	6	12
GO:0043933	protein-containing complex organization	4	12
GO:0065003	protein-containing complex assembly	5	12
GO:0065004	protein-DNA complex assembly	6	12
GO:0071824	protein-DNA complex subunit organization	5	12
GO:0071840	cellular component organization or biogenesis	2	12

Molecular functions
Term	Name	Level	Count
GO:0003682	chromatin binding	2	1
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0042393	histone binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	152	156	PF00656	0.427
CLV_C14_Caspase3-7	9	13	PF00656	0.402
CLV_NRD_NRD_1	236	238	PF00675	0.338
CLV_NRD_NRD_1	40	42	PF00675	0.418
CLV_PCSK_KEX2_1	176	178	PF00082	0.200
CLV_PCSK_KEX2_1	40	42	PF00082	0.340
CLV_PCSK_PC1ET2_1	176	178	PF00082	0.200
CLV_PCSK_SKI1_1	237	241	PF00082	0.322
CLV_PCSK_SKI1_1	275	279	PF00082	0.578
DEG_APCC_DBOX_1	236	244	PF00400	0.342
DEG_Nend_UBRbox_2	1	3	PF02207	0.526
DEG_SPOP_SBC_1	189	193	PF00917	0.382
DOC_MAPK_gen_1	102	110	PF00069	0.464
DOC_MAPK_MEF2A_6	102	110	PF00069	0.478
DOC_PP4_FxxP_1	79	82	PF00568	0.478
DOC_USP7_MATH_1	123	127	PF00917	0.390
DOC_USP7_MATH_1	142	146	PF00917	0.374
DOC_USP7_MATH_1	178	182	PF00917	0.444
DOC_USP7_MATH_1	187	191	PF00917	0.411
DOC_WW_Pin1_4	73	78	PF00397	0.503
LIG_14-3-3_CanoR_1	105	109	PF00244	0.417
LIG_Actin_WH2_2	241	259	PF00022	0.356
LIG_APCC_ABBA_1	199	204	PF00400	0.409
LIG_APCC_ABBA_1	205	210	PF00400	0.387
LIG_APCC_ABBA_1	80	85	PF00400	0.478
LIG_BRCT_BRCA1_1	144	148	PF00533	0.465
LIG_deltaCOP1_diTrp_1	143	153	PF00928	0.478
LIG_EH_1	112	116	PF12763	0.385
LIG_FHA_2	150	156	PF00498	0.497
LIG_FHA_2	88	94	PF00498	0.453
LIG_LIR_Apic_2	76	82	PF02991	0.274
LIG_LIR_Gen_1	200	209	PF02991	0.390
LIG_LIR_Nem_3	145	151	PF02991	0.404
LIG_NRBOX	61	67	PF00104	0.380
LIG_PTB_Apo_2	110	117	PF02174	0.385
LIG_RPA_C_Fungi	36	48	PF08784	0.251
LIG_SH2_STAP1	83	87	PF00017	0.257
LIG_SH2_STAT5	91	94	PF00017	0.231
LIG_SH2_STAT5	99	102	PF00017	0.214
LIG_SH3_3	203	209	PF00018	0.303
LIG_SUMO_SIM_anti_2	93	101	PF11976	0.274
LIG_SUMO_SIM_par_1	197	204	PF11976	0.355
LIG_TRAF2_1	133	136	PF00917	0.383
LIG_TRAF2_1	141	144	PF00917	0.318
LIG_TRAF2_1	226	229	PF00917	0.517
LIG_UBA3_1	62	69	PF00899	0.412
MOD_CK1_1	149	155	PF00069	0.209
MOD_CK1_1	188	194	PF00069	0.305
MOD_CK1_1	73	79	PF00069	0.427
MOD_CK2_1	194	200	PF00069	0.281
MOD_CK2_1	264	270	PF00069	0.525
MOD_CK2_1	87	93	PF00069	0.349
MOD_Cter_Amidation	173	176	PF01082	0.336
MOD_GlcNHglycan	148	151	PF01048	0.224
MOD_GlcNHglycan	181	184	PF01048	0.299
MOD_GlcNHglycan	187	190	PF01048	0.311
MOD_GlcNHglycan	253	256	PF01048	0.541
MOD_GSK3_1	142	149	PF00069	0.280
MOD_GSK3_1	185	192	PF00069	0.261
MOD_GSK3_1	260	267	PF00069	0.590
MOD_GSK3_1	6	13	PF00069	0.469
MOD_NEK2_1	185	190	PF00069	0.263
MOD_NEK2_1	87	92	PF00069	0.354
MOD_PIKK_1	190	196	PF00454	0.355
MOD_PKA_2	104	110	PF00069	0.268
MOD_PKA_2	70	76	PF00069	0.506
MOD_Plk_1	142	148	PF00069	0.297
MOD_Plk_4	194	200	PF00069	0.303
MOD_Plk_4	87	93	PF00069	0.317
MOD_ProDKin_1	73	79	PF00069	0.495
MOD_SUMO_for_1	20	23	PF00179	0.348
MOD_SUMO_for_1	277	280	PF00179	0.567
MOD_SUMO_for_1	49	52	PF00179	0.462
MOD_SUMO_rev_2	23	30	PF00179	0.313
MOD_SUMO_rev_2	231	240	PF00179	0.392
TRG_ER_diLys_1	276	280	PF00400	0.557
TRG_NLS_MonoExtC_3	274	280	PF00514	0.559
TRG_NLS_MonoExtN_4	173	179	PF00514	0.395

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCY5	Leptomonas seymouri	68%	98%
A0A0S4J5N0	Bodo saltans	34%	100%
A0A1X0NW78	Trypanosomatidae	33%	81%
A0A3Q8IAW7	Leishmania donovani	99%	100%
A0A3R7M0B2	Trypanosoma rangeli	38%	94%
C9ZIF4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	89%
E9AII4	Leishmania braziliensis	78%	100%
E9AUR9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
Q4QCR7	Leishmania major	96%	100%
V5D5V0	Trypanosoma cruzi	37%	91%