Putative calpain-like cysteine peptidase

Quick info Annotations Localization Phosphorylation Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

small myristoylated protein 4 - putative

Species:

Leishmania infantum

UniProt:

A4HYX1_LEIIN

TriTrypDb:

LINF_200018000

Length:

151

Annotations

LeishMANIAdb interaction annotations

by homology
Contact email: avilan@ula.ve
Publication title: Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana
Publication 1st author(s): Figuera
Publication Identifier(s): 23178693
Host organism: -1
Interaction detection method(s): enzymatic linked imunosorbent assay
Interaction type: direct interaction
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: E9AUS1
ID(s) interactor B: P00747
Taxid interactor A: Leishmania mexicana
Taxid interactor B: Homo sapiens
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: bait

Annotations by Jardim et al.

Proteases, calpain-like cysteine peptidase

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 2
Forrest at al. (metacyclic)	yes	yes: 5
Forrest at al. (procyclic)	yes	yes: 7
Silverman et al.	no	yes: 1
Pissara et al.	yes	yes: 24

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 12

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 27

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 46
NetGPI	no	yes: 0, no: 46

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0097542	ciliary tip	2	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	2
GO:0020018	ciliary pocket membrane	6	1
GO:0031090	organelle membrane	3	2
GO:0031253	cell projection membrane	4	2
GO:0060170	ciliary membrane	5	2
GO:0098588	bounding membrane of organelle	4	2
GO:0098590	plasma membrane region	3	2

Phosphorylation

Promastigote: 148, 149

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A4HYX1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HYX1

PDB structure(s): 1r75_A , 2fe0_A

Function

Biological processes
Term	Name	Level	Count
GO:0001539	cilium or flagellum-dependent cell motility	3	1
GO:0009987	cellular process	1	2
GO:0048870	cell motility	2	1
GO:0060285	cilium-dependent cell motility	4	1
GO:0006996	organelle organization	4	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0030030	cell projection organization	4	1
GO:0030031	cell projection assembly	5	1
GO:0044000	movement in host	4	1
GO:0044403	biological process involved in symbiotic interaction	2	1
GO:0044409	entry into host	5	1
GO:0044419	biological process involved in interspecies interaction between organisms	1	1
GO:0044782	cilium organization	5	1
GO:0051701	biological process involved in interaction with host	3	1
GO:0060271	cilium assembly	6	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0120031	plasma membrane bounded cell projection assembly	6	1
GO:0120036	plasma membrane bounded cell projection organization	5	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	81	85	PF00656	0.236
CLV_NRD_NRD_1	125	127	PF00675	0.348
CLV_PCSK_KEX2_1	125	127	PF00082	0.365
CLV_PCSK_KEX2_1	130	132	PF00082	0.403
CLV_PCSK_KEX2_1	54	56	PF00082	0.372
CLV_PCSK_PC1ET2_1	130	132	PF00082	0.423
CLV_PCSK_PC1ET2_1	54	56	PF00082	0.343
CLV_PCSK_PC7_1	126	132	PF00082	0.387
CLV_PCSK_SKI1_1	131	135	PF00082	0.564
DOC_MAPK_gen_1	54	62	PF00069	0.378
DOC_MAPK_MEF2A_6	55	64	PF00069	0.376
DOC_PP1_RVXF_1	59	65	PF00149	0.313
DOC_USP7_UBL2_3	123	127	PF12436	0.442
DOC_USP7_UBL2_3	130	134	PF12436	0.484
LIG_APCC_ABBA_1	26	31	PF00400	0.414
LIG_APCC_ABBAyCdc20_2	25	31	PF00400	0.185
LIG_BRCT_BRCA1_1	45	49	PF00533	0.174
LIG_LIR_Apic_2	11	17	PF02991	0.506
LIG_LIR_Nem_3	45	50	PF02991	0.331
LIG_PCNA_yPIPBox_3	110	123	PF02747	0.270
LIG_PCNA_yPIPBox_3	32	40	PF02747	0.315
LIG_SH2_CRK	14	18	PF00017	0.495
LIG_SH2_STAP1	22	26	PF00017	0.169
LIG_SH2_STAT5	78	81	PF00017	0.337
MOD_CK1_1	15	21	PF00069	0.389
MOD_GlcNHglycan	14	17	PF01048	0.286
MOD_N-GLC_1	43	48	PF02516	0.308
MOD_N-GLC_2	51	53	PF02516	0.174
MOD_NEK2_1	12	17	PF00069	0.469
MOD_NEK2_1	146	151	PF00069	0.450
MOD_NEK2_1	74	79	PF00069	0.304
MOD_NMyristoyl	1	7	PF02799	0.567
MOD_PKA_2	12	18	PF00069	0.438
MOD_Plk_1	43	49	PF00069	0.369
MOD_Plk_4	108	114	PF00069	0.142
MOD_Plk_4	74	80	PF00069	0.296
MOD_Plk_4	88	94	PF00069	0.270
MOD_SUMO_rev_2	124	132	PF00179	0.508
MOD_SUMO_rev_2	19	27	PF00179	0.185
MOD_SUMO_rev_2	46	56	PF00179	0.439
TRG_DiLeu_BaEn_1	31	36	PF01217	0.232

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6Y3	Leptomonas seymouri	44%	100%
A0A0N1HYV4	Leptomonas seymouri	43%	100%
A0A0N1I5S6	Leptomonas seymouri	71%	98%
A0A0N1PCZ0	Leptomonas seymouri	42%	100%
A0A0N1PE11	Leptomonas seymouri	35%	100%
A0A1X0NW79	Trypanosomatidae	47%	76%
A0A1X0NW95	Trypanosomatidae	45%	100%
A0A1X0NWH2	Trypanosomatidae	32%	100%
A0A1X0NWV3	Trypanosomatidae	49%	100%
A0A1X0NXT9	Trypanosomatidae	35%	100%
A0A1X0NYV3	Trypanosomatidae	40%	100%
A0A3Q8I9V9	Leishmania donovani	45%	100%
A0A3Q8IBM6	Leishmania donovani	31%	100%
A0A3Q8IDP8	Leishmania donovani	100%	100%
A0A3Q8IKN9	Leishmania donovani	42%	100%
A0A3R7MB04	Trypanosoma rangeli	39%	100%
A0A3R7MWC6	Trypanosoma rangeli	42%	100%
A0A3S7WW23	Leishmania donovani	39%	100%
A0A3S7WZ86	Leishmania donovani	34%	100%
A0A422MSY8	Trypanosoma rangeli	41%	90%
A0A422MSZ3	Trypanosoma rangeli	43%	100%
A0A422MUY0	Trypanosoma rangeli	51%	100%
A4H7N9	Leishmania braziliensis	44%	100%
A4H7P0	Leishmania braziliensis	44%	100%
A4HEG7	Leishmania braziliensis	36%	100%
A4HW29	Leishmania infantum	45%	100%
A4HYW6	Leishmania infantum	31%	100%
A4HYX3	Leishmania infantum	39%	100%
A4HYX4	Leishmania infantum	42%	100%
A4I1M3	Leishmania infantum	34%	100%
C9ZIF6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
E9AII3	Leishmania braziliensis	83%	100%
E9AII5	Leishmania braziliensis	41%	100%
E9AII6	Leishmania braziliensis	46%	100%
E9APT0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	45%	100%
E9AUR8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
E9AUS0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
E9AUS1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	45%	100%
E9AXQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
P84150	Leishmania major	32%	100%
Q4Q9R4	Leishmania major	35%	100%
Q4QFP8	Leishmania major	45%	100%
Q5SDH3	Leishmania major	97%	100%
Q5SDH4	Leishmania major	39%	100%
Q5SDH5	Leishmania major	46%	100%