Putative RNA polymerase subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA-directed RNA polymerase 2 - RPB12 subunit - putative

Species:

Leishmania infantum

UniProt:

A4HYR2_LEIIN

TriTrypDb:

LINF_200010300

Length:

142

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 5

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	1
GO:0030880	RNA polymerase complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	1
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990234	transferase complex	3	1

Expansion

Sequence features

A4HYR2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HYR2

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006351	DNA-templated transcription	7	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009059	macromolecule biosynthetic process	4	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0032774	RNA biosynthetic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034654	nucleobase-containing compound biosynthetic process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:0097659	nucleic acid-templated transcription	6	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	5
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	5
GO:0016740	transferase activity	2	5
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	5
GO:0016779	nucleotidyltransferase activity	4	5
GO:0034062	5'-3' RNA polymerase activity	5	5
GO:0097747	RNA polymerase activity	4	5
GO:0140098	catalytic activity, acting on RNA	3	5
GO:0140640	catalytic activity, acting on a nucleic acid	2	5
GO:0003676	nucleic acid binding	3	1
GO:0003677	DNA binding	4	1
GO:0005488	binding	1	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	45	47	PF00675	0.549
CLV_PCSK_KEX2_1	45	47	PF00082	0.560
DOC_MAPK_gen_1	45	53	PF00069	0.538
DOC_MAPK_gen_1	89	98	PF00069	0.544
DOC_USP7_MATH_1	117	121	PF00917	0.572
DOC_USP7_MATH_1	8	12	PF00917	0.546
DOC_USP7_UBL2_3	54	58	PF12436	0.586
LIG_BIR_II_1	1	5	PF00653	0.484
LIG_BRCT_BRCA1_1	125	129	PF00533	0.470
LIG_BRCT_BRCA1_2	125	131	PF00533	0.471
LIG_FHA_1	101	107	PF00498	0.405
LIG_FHA_1	38	44	PF00498	0.536
LIG_FHA_1	93	99	PF00498	0.476
LIG_FHA_2	135	141	PF00498	0.499
LIG_FHA_2	81	87	PF00498	0.714
LIG_LIR_Gen_1	137	142	PF02991	0.510
LIG_LIR_Nem_3	126	132	PF02991	0.457
LIG_LIR_Nem_3	137	142	PF02991	0.510
LIG_MYND_1	29	33	PF01753	0.445
LIG_PDZ_Class_3	137	142	PF00595	0.510
LIG_SH2_STAT5	107	110	PF00017	0.542
LIG_SH2_STAT5	97	100	PF00017	0.404
LIG_SH3_3	2	8	PF00018	0.521
LIG_TRAF2_1	69	72	PF00917	0.719
LIG_TRAF2_1	79	82	PF00917	0.481
LIG_TYR_ITSM	135	142	PF00017	0.506
MOD_CK1_1	120	126	PF00069	0.619
MOD_GlcNHglycan	1	4	PF01048	0.532
MOD_GlcNHglycan	48	51	PF01048	0.746
MOD_GSK3_1	120	127	PF00069	0.493
MOD_GSK3_1	131	138	PF00069	0.482
MOD_N-GLC_1	100	105	PF02516	0.427
MOD_NEK2_2	124	129	PF00069	0.475
MOD_PIKK_1	67	73	PF00454	0.716
MOD_PIKK_1	77	83	PF00454	0.485
MOD_Plk_2-3	135	141	PF00069	0.553
MOD_Plk_4	124	130	PF00069	0.474
MOD_Plk_4	92	98	PF00069	0.450
MOD_SUMO_for_1	53	56	PF00179	0.583
MOD_SUMO_for_1	57	60	PF00179	0.595
TRG_ENDOCYTIC_2	139	142	PF00928	0.527
TRG_ER_diArg_1	45	47	PF00400	0.528

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A3Q8IBK2	Leishmania donovani	99%	69%
C9ZI68	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	88%
E9AIA6	Leishmania braziliensis	78%	100%
E9AUJ3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
Q4QCZ1	Leishmania major	93%	100%