Checkpoint protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Checkpoint protein

Gene product:

Cell cycle checkpoint protein RAD1-like - putative

Species:

Leishmania infantum

UniProt:

A4HYN1_LEIIN

TriTrypDb:

LINF_200009300

Length:

248

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0030896	checkpoint clamp complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

A4HYN1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HYN1

Function

Biological processes
Term	Name	Level	Count
GO:0000075	cell cycle checkpoint signaling	4	12
GO:0000077	DNA damage checkpoint signaling	5	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006281	DNA repair	5	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0006974	DNA damage response	4	12
GO:0007165	signal transduction	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0010564	regulation of cell cycle process	5	12
GO:0010948	negative regulation of cell cycle process	6	12
GO:0031570	DNA integrity checkpoint signaling	5	12
GO:0033554	cellular response to stress	3	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0035556	intracellular signal transduction	3	12
GO:0042770	signal transduction in response to DNA damage	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0045786	negative regulation of cell cycle	5	12
GO:0046483	heterocycle metabolic process	3	12
GO:0048519	negative regulation of biological process	3	12
GO:0048523	negative regulation of cellular process	4	12
GO:0050789	regulation of biological process	2	12
GO:0050794	regulation of cellular process	3	12
GO:0050896	response to stimulus	1	12
GO:0051716	cellular response to stimulus	2	12
GO:0051726	regulation of cell cycle	4	12
GO:0065007	biological regulation	1	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901987	regulation of cell cycle phase transition	6	12
GO:1901988	negative regulation of cell cycle phase transition	7	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0004518	nuclease activity	4	1
GO:0004527	exonuclease activity	5	1
GO:0004529	DNA exonuclease activity	5	1
GO:0004536	DNA nuclease activity	4	1
GO:0008853	obsolete exodeoxyribonuclease III activity	7	1
GO:0016787	hydrolase activity	2	1
GO:0016788	hydrolase activity, acting on ester bonds	3	1
GO:0016796	exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	1
GO:0016895	DNA exonuclease activity, producing 5'-phosphomonoesters	6	1
GO:0140097	catalytic activity, acting on DNA	3	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	75	79	PF00656	0.349
CLV_MEL_PAP_1	157	163	PF00089	0.452
CLV_PCSK_SKI1_1	56	60	PF00082	0.387
CLV_PCSK_SKI1_1	68	72	PF00082	0.399
CLV_PCSK_SKI1_1	93	97	PF00082	0.457
DEG_APCC_DBOX_1	152	160	PF00400	0.461
DOC_CYCLIN_RxL_1	53	61	PF00134	0.447
DOC_MAPK_gen_1	150	159	PF00069	0.418
DOC_MAPK_HePTP_8	192	204	PF00069	0.406
DOC_MAPK_MEF2A_6	153	161	PF00069	0.432
DOC_MAPK_MEF2A_6	195	204	PF00069	0.500
DOC_MAPK_MEF2A_6	6	14	PF00069	0.362
DOC_PP2B_LxvP_1	71	74	PF13499	0.425
DOC_USP7_MATH_1	190	194	PF00917	0.451
DOC_USP7_MATH_1	88	92	PF00917	0.486
DOC_WW_Pin1_4	107	112	PF00397	0.508
DOC_WW_Pin1_4	6	11	PF00397	0.473
LIG_14-3-3_CanoR_1	141	147	PF00244	0.521
LIG_14-3-3_CanoR_1	15	21	PF00244	0.517
LIG_14-3-3_CanoR_1	160	167	PF00244	0.542
LIG_14-3-3_CanoR_1	237	245	PF00244	0.394
LIG_Actin_WH2_2	145	162	PF00022	0.525
LIG_APCC_ABBA_1	37	42	PF00400	0.367
LIG_BIR_II_1	1	5	PF00653	0.333
LIG_deltaCOP1_diTrp_1	25	32	PF00928	0.329
LIG_FHA_1	110	116	PF00498	0.457
LIG_FHA_1	147	153	PF00498	0.407
LIG_FHA_1	17	23	PF00498	0.414
LIG_FHA_1	238	244	PF00498	0.421
LIG_FHA_1	49	55	PF00498	0.384
LIG_FHA_1	90	96	PF00498	0.433
LIG_FHA_2	108	114	PF00498	0.439
LIG_FHA_2	129	135	PF00498	0.673
LIG_FHA_2	174	180	PF00498	0.405
LIG_FHA_2	73	79	PF00498	0.391
LIG_LIR_Gen_1	2	12	PF02991	0.358
LIG_LIR_Gen_1	25	32	PF02991	0.344
LIG_LIR_Gen_1	61	70	PF02991	0.481
LIG_LIR_Nem_3	2	7	PF02991	0.342
LIG_LIR_Nem_3	25	30	PF02991	0.390
LIG_LIR_Nem_3	61	65	PF02991	0.423
LIG_NRBOX	142	148	PF00104	0.511
LIG_SH2_CRK	4	8	PF00017	0.355
LIG_SH2_NCK_1	62	66	PF00017	0.492
LIG_SH2_STAP1	239	243	PF00017	0.503
LIG_SH2_STAT5	239	242	PF00017	0.513
LIG_SH2_STAT5	4	7	PF00017	0.342
LIG_SUMO_SIM_par_1	105	110	PF11976	0.340
LIG_SUMO_SIM_par_1	18	23	PF11976	0.411
LIG_SUMO_SIM_par_1	221	229	PF11976	0.538
MOD_CDK_SPxxK_3	107	114	PF00069	0.466
MOD_CK1_1	233	239	PF00069	0.426
MOD_CK1_1	86	92	PF00069	0.549
MOD_GlcNHglycan	124	127	PF01048	0.559
MOD_GlcNHglycan	163	166	PF01048	0.389
MOD_GlcNHglycan	231	235	PF01048	0.545
MOD_GSK3_1	142	149	PF00069	0.421
MOD_GSK3_1	16	23	PF00069	0.411
MOD_GSK3_1	226	233	PF00069	0.535
MOD_GSK3_1	26	33	PF00069	0.295
MOD_GSK3_1	46	53	PF00069	0.417
MOD_N-GLC_1	46	51	PF02516	0.533
MOD_NEK2_1	142	147	PF00069	0.406
MOD_NEK2_1	161	166	PF00069	0.461
MOD_NEK2_1	32	37	PF00069	0.430
MOD_NEK2_1	48	53	PF00069	0.515
MOD_NEK2_2	190	195	PF00069	0.280
MOD_PIKK_1	128	134	PF00454	0.536
MOD_PKA_2	159	165	PF00069	0.540
MOD_Plk_1	190	196	PF00069	0.460
MOD_Plk_4	142	148	PF00069	0.481
MOD_Plk_4	26	32	PF00069	0.378
MOD_Plk_4	72	78	PF00069	0.412
MOD_ProDKin_1	107	113	PF00069	0.522
MOD_ProDKin_1	6	12	PF00069	0.477
MOD_SUMO_rev_2	101	106	PF00179	0.444
MOD_SUMO_rev_2	191	200	PF00179	0.412
TRG_ENDOCYTIC_2	211	214	PF00928	0.511
TRG_ENDOCYTIC_2	4	7	PF00928	0.342
TRG_ENDOCYTIC_2	62	65	PF00928	0.499
TRG_Pf-PMV_PEXEL_1	104	108	PF00026	0.341
TRG_Pf-PMV_PEXEL_1	178	183	PF00026	0.498

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HUB9	Leptomonas seymouri	73%	71%
A0A0S4J1V0	Bodo saltans	35%	83%
A0A1X0NWX4	Trypanosomatidae	47%	81%
A0A3S7WVU5	Leishmania donovani	100%	69%
A0A422NCM6	Trypanosoma rangeli	47%	82%
C9ZI59	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	81%
E9AI98	Leishmania braziliensis	91%	100%
E9AUI3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
Q4QD01	Leishmania major	97%	100%
V5BVI1	Trypanosoma cruzi	47%	82%