ZZ-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

E3 ubiquitin-protein ligase KCMF1 - putative

Species:

Leishmania infantum

UniProt:

A4HYM1_LEIIN

TriTrypDb:

LINF_200008000

Length:

799

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005622	intracellular anatomical structure	2	1
GO:0005737	cytoplasm	2	1
GO:0005773	vacuole	5	1
GO:0005776	autophagosome	6	1
GO:0016234	inclusion body	3	1
GO:0016235	aggresome	4	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0044753	amphisome	7	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HYM1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HYM1

Function

Biological processes
Term	Name	Level	Count
GO:0000422	autophagy of mitochondrion	4	1
GO:0000423	mitophagy	5	1
GO:0006914	autophagy	3	1
GO:0006996	organelle organization	4	1
GO:0007005	mitochondrion organization	5	1
GO:0007032	endosome organization	6	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0016050	vesicle organization	5	1
GO:0016236	macroautophagy	4	1
GO:0022411	cellular component disassembly	4	1
GO:0035973	aggrephagy	6	1
GO:0044237	cellular metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0061726	mitochondrion disassembly	6	1
GO:0061912	selective autophagy	5	1
GO:0061919	process utilizing autophagic mechanism	2	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:1903008	organelle disassembly	5	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	11
GO:0008270	zinc ion binding	6	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0046914	transition metal ion binding	5	11
GO:0005080	protein kinase C binding	6	1
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0019900	kinase binding	4	1
GO:0019901	protein kinase binding	5	1
GO:0031593	polyubiquitin modification-dependent protein binding	4	1
GO:0070530	K63-linked polyubiquitin modification-dependent protein binding	5	1
GO:0140030	modification-dependent protein binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	472	476	PF00656	0.488
CLV_NRD_NRD_1	180	182	PF00675	0.557
CLV_NRD_NRD_1	280	282	PF00675	0.383
CLV_NRD_NRD_1	430	432	PF00675	0.526
CLV_NRD_NRD_1	460	462	PF00675	0.593
CLV_NRD_NRD_1	500	502	PF00675	0.543
CLV_NRD_NRD_1	71	73	PF00675	0.455
CLV_PCSK_FUR_1	458	462	PF00082	0.569
CLV_PCSK_KEX2_1	280	282	PF00082	0.440
CLV_PCSK_KEX2_1	341	343	PF00082	0.381
CLV_PCSK_KEX2_1	37	39	PF00082	0.412
CLV_PCSK_KEX2_1	430	432	PF00082	0.512
CLV_PCSK_KEX2_1	458	460	PF00082	0.668
CLV_PCSK_KEX2_1	500	502	PF00082	0.462
CLV_PCSK_KEX2_1	71	73	PF00082	0.417
CLV_PCSK_KEX2_1	721	723	PF00082	0.506
CLV_PCSK_PC1ET2_1	341	343	PF00082	0.381
CLV_PCSK_PC1ET2_1	37	39	PF00082	0.412
CLV_PCSK_PC1ET2_1	721	723	PF00082	0.564
CLV_PCSK_PC7_1	717	723	PF00082	0.520
CLV_PCSK_SKI1_1	281	285	PF00082	0.450
CLV_PCSK_SKI1_1	71	75	PF00082	0.438
CLV_PCSK_SKI1_1	84	88	PF00082	0.288
DEG_APCC_DBOX_1	250	258	PF00400	0.468
DEG_SPOP_SBC_1	170	174	PF00917	0.589
DEG_SPOP_SBC_1	66	70	PF00917	0.385
DOC_CKS1_1	123	128	PF01111	0.503
DOC_CKS1_1	583	588	PF01111	0.507
DOC_CYCLIN_RxL_1	278	287	PF00134	0.501
DOC_CYCLIN_RxL_1	495	507	PF00134	0.475
DOC_CYCLIN_yClb1_LxF_4	204	210	PF00134	0.474
DOC_CYCLIN_yCln2_LP_2	120	126	PF00134	0.432
DOC_CYCLIN_yCln2_LP_2	646	652	PF00134	0.351
DOC_MAPK_gen_1	341	348	PF00069	0.355
DOC_MAPK_gen_1	436	444	PF00069	0.412
DOC_MAPK_gen_1	459	471	PF00069	0.552
DOC_MAPK_gen_1	500	506	PF00069	0.439
DOC_MAPK_MEF2A_6	341	350	PF00069	0.325
DOC_MAPK_MEF2A_6	464	471	PF00069	0.562
DOC_PP2B_LxvP_1	646	649	PF13499	0.464
DOC_PP2B_LxvP_1	770	773	PF13499	0.482
DOC_PP2B_LxvP_1	784	787	PF13499	0.561
DOC_PP4_FxxP_1	783	786	PF00568	0.502
DOC_USP7_MATH_1	170	174	PF00917	0.629
DOC_USP7_MATH_1	231	235	PF00917	0.604
DOC_USP7_MATH_1	519	523	PF00917	0.594
DOC_USP7_MATH_1	576	580	PF00917	0.497
DOC_USP7_MATH_1	678	682	PF00917	0.475
DOC_USP7_MATH_1	759	763	PF00917	0.599
DOC_USP7_UBL2_3	337	341	PF12436	0.557
DOC_WW_Pin1_4	122	127	PF00397	0.435
DOC_WW_Pin1_4	191	196	PF00397	0.666
DOC_WW_Pin1_4	370	375	PF00397	0.433
DOC_WW_Pin1_4	451	456	PF00397	0.538
DOC_WW_Pin1_4	5	10	PF00397	0.565
DOC_WW_Pin1_4	582	587	PF00397	0.473
DOC_WW_Pin1_4	744	749	PF00397	0.640
DOC_WW_Pin1_4	751	756	PF00397	0.623
LIG_14-3-3_CanoR_1	203	208	PF00244	0.442
LIG_14-3-3_CanoR_1	251	255	PF00244	0.356
LIG_14-3-3_CanoR_1	517	525	PF00244	0.421
LIG_14-3-3_CanoR_1	691	696	PF00244	0.384
LIG_14-3-3_CanoR_1	95	100	PF00244	0.356
LIG_Actin_WH2_2	29	47	PF00022	0.421
LIG_AP2alpha_2	309	311	PF02296	0.372
LIG_AP2alpha_2	523	525	PF02296	0.484
LIG_APCC_ABBAyCdc20_2	280	286	PF00400	0.424
LIG_BIR_II_1	1	5	PF00653	0.580
LIG_Clathr_ClatBox_1	347	351	PF01394	0.311
LIG_Clathr_ClatBox_1	395	399	PF01394	0.413
LIG_deltaCOP1_diTrp_1	277	284	PF00928	0.489
LIG_deltaCOP1_diTrp_1	309	314	PF00928	0.379
LIG_DLG_GKlike_1	691	699	PF00625	0.324
LIG_FHA_1	105	111	PF00498	0.468
LIG_FHA_1	123	129	PF00498	0.263
LIG_FHA_1	328	334	PF00498	0.545
LIG_FHA_1	439	445	PF00498	0.546
LIG_FHA_1	466	472	PF00498	0.651
LIG_FHA_1	505	511	PF00498	0.368
LIG_FHA_1	68	74	PF00498	0.431
LIG_FHA_1	752	758	PF00498	0.692
LIG_FHA_1	787	793	PF00498	0.425
LIG_FHA_2	184	190	PF00498	0.771
LIG_FHA_2	244	250	PF00498	0.474
LIG_FHA_2	357	363	PF00498	0.554
LIG_FHA_2	480	486	PF00498	0.588
LIG_FHA_2	542	548	PF00498	0.439
LIG_IBAR_NPY_1	518	520	PF08397	0.488
LIG_Integrin_RGD_1	735	737	PF01839	0.564
LIG_LIR_Apic_2	782	786	PF02991	0.515
LIG_LIR_Gen_1	19	26	PF02991	0.468
LIG_LIR_Gen_1	213	222	PF02991	0.633
LIG_LIR_Gen_1	299	310	PF02991	0.501
LIG_LIR_Gen_1	30	39	PF02991	0.376
LIG_LIR_Gen_1	359	368	PF02991	0.445
LIG_LIR_Gen_1	475	483	PF02991	0.482
LIG_LIR_Gen_1	533	543	PF02991	0.350
LIG_LIR_Nem_3	19	23	PF02991	0.424
LIG_LIR_Nem_3	213	217	PF02991	0.486
LIG_LIR_Nem_3	25	29	PF02991	0.367
LIG_LIR_Nem_3	299	305	PF02991	0.505
LIG_LIR_Nem_3	30	36	PF02991	0.338
LIG_LIR_Nem_3	309	314	PF02991	0.432
LIG_LIR_Nem_3	359	364	PF02991	0.425
LIG_LIR_Nem_3	475	481	PF02991	0.449
LIG_LIR_Nem_3	522	528	PF02991	0.493
LIG_LIR_Nem_3	533	538	PF02991	0.254
LIG_PCNA_PIPBox_1	389	398	PF02747	0.475
LIG_Pex14_1	136	140	PF04695	0.371
LIG_Pex14_1	268	272	PF04695	0.429
LIG_PTAP_UEV_1	572	577	PF05743	0.402
LIG_Rb_LxCxE_1	390	413	PF01857	0.261
LIG_SH2_CRK	302	306	PF00017	0.480
LIG_SH2_CRK	76	80	PF00017	0.386
LIG_SH2_PTP2	535	538	PF00017	0.327
LIG_SH2_STAP1	210	214	PF00017	0.527
LIG_SH2_STAP1	302	306	PF00017	0.480
LIG_SH2_STAP1	719	723	PF00017	0.594
LIG_SH2_STAT5	140	143	PF00017	0.524
LIG_SH2_STAT5	514	517	PF00017	0.584
LIG_SH2_STAT5	520	523	PF00017	0.528
LIG_SH2_STAT5	535	538	PF00017	0.372
LIG_SH2_STAT5	542	545	PF00017	0.383
LIG_SH2_STAT5	688	691	PF00017	0.294
LIG_SH2_STAT5	81	84	PF00017	0.448
LIG_SH3_2	323	328	PF14604	0.535
LIG_SH3_3	120	126	PF00018	0.451
LIG_SH3_3	320	326	PF00018	0.497
LIG_SH3_3	570	576	PF00018	0.404
LIG_SH3_3	649	655	PF00018	0.479
LIG_SH3_3	738	744	PF00018	0.712
LIG_SH3_3	747	753	PF00018	0.591
LIG_SH3_4	337	344	PF00018	0.331
LIG_SUMO_SIM_anti_2	375	380	PF11976	0.427
LIG_SUMO_SIM_par_1	431	438	PF11976	0.410
LIG_SUMO_SIM_par_1	467	473	PF11976	0.591
LIG_TRAF2_1	8	11	PF00917	0.547
LIG_TYR_ITIM	74	79	PF00017	0.402
LIG_UBA3_1	335	341	PF00899	0.544
MOD_CDC14_SPxK_1	194	197	PF00782	0.504
MOD_CDC14_SPxK_1	749	752	PF00782	0.578
MOD_CDK_SPK_2	451	456	PF00069	0.557
MOD_CDK_SPxK_1	191	197	PF00069	0.509
MOD_CDK_SPxK_1	746	752	PF00069	0.579
MOD_CDK_SPxxK_3	451	458	PF00069	0.622
MOD_CDK_SPxxK_3	582	589	PF00069	0.588
MOD_CK1_1	127	133	PF00069	0.494
MOD_CK1_1	169	175	PF00069	0.564
MOD_CK1_1	2	8	PF00069	0.609
MOD_CK1_1	234	240	PF00069	0.393
MOD_CK1_1	413	419	PF00069	0.708
MOD_CK1_1	438	444	PF00069	0.436
MOD_CK1_1	622	628	PF00069	0.588
MOD_CK1_1	725	731	PF00069	0.600
MOD_CK1_1	746	752	PF00069	0.694
MOD_CK1_1	779	785	PF00069	0.655
MOD_CK2_1	171	177	PF00069	0.683
MOD_CK2_1	243	249	PF00069	0.422
MOD_CK2_1	356	362	PF00069	0.503
MOD_CK2_1	479	485	PF00069	0.531
MOD_CK2_1	5	11	PF00069	0.557
MOD_CK2_1	566	572	PF00069	0.532
MOD_GlcNHglycan	168	171	PF01048	0.572
MOD_GlcNHglycan	200	203	PF01048	0.659
MOD_GlcNHglycan	298	301	PF01048	0.510
MOD_GlcNHglycan	39	42	PF01048	0.432
MOD_GlcNHglycan	572	576	PF01048	0.411
MOD_GlcNHglycan	609	613	PF01048	0.327
MOD_GlcNHglycan	623	627	PF01048	0.363
MOD_GlcNHglycan	778	781	PF01048	0.551
MOD_GSK3_1	1	8	PF00069	0.678
MOD_GSK3_1	166	173	PF00069	0.568
MOD_GSK3_1	183	190	PF00069	0.757
MOD_GSK3_1	243	250	PF00069	0.431
MOD_GSK3_1	387	394	PF00069	0.420
MOD_GSK3_1	412	419	PF00069	0.644
MOD_GSK3_1	434	441	PF00069	0.529
MOD_GSK3_1	465	472	PF00069	0.648
MOD_GSK3_1	591	598	PF00069	0.448
MOD_GSK3_1	618	625	PF00069	0.436
MOD_GSK3_1	723	730	PF00069	0.479
MOD_GSK3_1	742	749	PF00069	0.681
MOD_GSK3_1	759	766	PF00069	0.381
MOD_N-GLC_1	284	289	PF02516	0.409
MOD_N-GLC_1	426	431	PF02516	0.642
MOD_N-GLC_1	479	484	PF02516	0.576
MOD_N-GLC_1	566	571	PF02516	0.419
MOD_N-GLC_2	690	692	PF02516	0.294
MOD_NEK2_1	198	203	PF00069	0.678
MOD_NEK2_1	243	248	PF00069	0.449
MOD_NEK2_1	284	289	PF00069	0.432
MOD_NEK2_1	293	298	PF00069	0.473
MOD_NEK2_1	426	431	PF00069	0.469
MOD_NEK2_1	504	509	PF00069	0.440
MOD_NEK2_1	566	571	PF00069	0.406
MOD_NEK2_1	577	582	PF00069	0.390
MOD_NEK2_1	711	716	PF00069	0.362
MOD_NEK2_2	327	332	PF00069	0.454
MOD_PK_1	95	101	PF00069	0.372
MOD_PKA_1	37	43	PF00069	0.431
MOD_PKA_2	166	172	PF00069	0.612
MOD_PKA_2	234	240	PF00069	0.428
MOD_PKA_2	250	256	PF00069	0.488
MOD_PKA_2	37	43	PF00069	0.431
MOD_PKA_2	413	419	PF00069	0.559
MOD_PKA_2	435	441	PF00069	0.578
MOD_PKA_2	491	497	PF00069	0.463
MOD_PKA_2	696	702	PF00069	0.471
MOD_Plk_1	284	290	PF00069	0.518
MOD_Plk_1	474	480	PF00069	0.414
MOD_Plk_1	566	572	PF00069	0.481
MOD_Plk_1	622	628	PF00069	0.413
MOD_Plk_4	260	266	PF00069	0.334
MOD_Plk_4	377	383	PF00069	0.402
MOD_Plk_4	391	397	PF00069	0.399
MOD_Plk_4	531	537	PF00069	0.331
MOD_Plk_4	566	572	PF00069	0.479
MOD_Plk_4	779	785	PF00069	0.560
MOD_ProDKin_1	122	128	PF00069	0.443
MOD_ProDKin_1	191	197	PF00069	0.665
MOD_ProDKin_1	370	376	PF00069	0.424
MOD_ProDKin_1	451	457	PF00069	0.542
MOD_ProDKin_1	5	11	PF00069	0.562
MOD_ProDKin_1	582	588	PF00069	0.470
MOD_ProDKin_1	744	750	PF00069	0.638
MOD_ProDKin_1	751	757	PF00069	0.622
TRG_DiLeu_BaEn_1	213	218	PF01217	0.588
TRG_DiLeu_BaEn_1	53	58	PF01217	0.361
TRG_DiLeu_BaEn_2	398	404	PF01217	0.413
TRG_DiLeu_BaLyEn_6	655	660	PF01217	0.465
TRG_DiLeu_BaLyEn_6	69	74	PF01217	0.420
TRG_ENDOCYTIC_2	140	143	PF00928	0.389
TRG_ENDOCYTIC_2	26	29	PF00928	0.410
TRG_ENDOCYTIC_2	302	305	PF00928	0.536
TRG_ENDOCYTIC_2	361	364	PF00928	0.400
TRG_ENDOCYTIC_2	535	538	PF00928	0.327
TRG_ENDOCYTIC_2	76	79	PF00928	0.360
TRG_ER_diArg_1	279	281	PF00400	0.413
TRG_ER_diArg_1	430	432	PF00400	0.516
TRG_ER_diArg_1	457	460	PF00400	0.549
TRG_ER_diArg_1	500	502	PF00400	0.473
TRG_ER_diArg_1	71	73	PF00400	0.507
TRG_NES_CRM1_1	386	399	PF08389	0.480
TRG_NES_CRM1_1	560	572	PF08389	0.433
TRG_Pf-PMV_PEXEL_1	163	168	PF00026	0.506
TRG_Pf-PMV_PEXEL_1	281	286	PF00026	0.461

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJU9	Leptomonas seymouri	47%	100%
A0A0S4JDR0	Bodo saltans	26%	86%
A0A1X0NY20	Trypanosomatidae	31%	100%
A0A3S7WVR9	Leishmania donovani	99%	100%
A0A422NEI4	Trypanosoma rangeli	30%	100%
C9ZHT5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
E9AI85	Leishmania braziliensis	74%	98%
E9AUH0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q4QD14	Leishmania major	93%	100%