Vacuolar protein-sorting-associated protein 36

Quick info Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Vacuolar protein sorting protein 36 Vps36/EAP30/Vps36 family - putative

Species:

Leishmania infantum

UniProt:

A4HY94_LEIIN

TriTrypDb:

LINF_190012300

Length:

447

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	yes	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000814	ESCRT II complex	3	11
GO:0010008	endosome membrane	5	11
GO:0012506	vesicle membrane	4	11
GO:0016020	membrane	2	11
GO:0030659	cytoplasmic vesicle membrane	5	11
GO:0031090	organelle membrane	3	11
GO:0031902	late endosome membrane	6	11
GO:0032991	protein-containing complex	1	11
GO:0036452	ESCRT complex	2	11
GO:0098588	bounding membrane of organelle	4	11
GO:0098796	membrane protein complex	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0005737	cytoplasm	2	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A4HY94

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HY94

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	11
GO:0006886	intracellular protein transport	4	11
GO:0007034	vacuolar transport	4	11
GO:0008104	protein localization	4	11
GO:0009987	cellular process	1	11
GO:0015031	protein transport	4	11
GO:0016192	vesicle-mediated transport	4	11
GO:0016197	endosomal transport	4	11
GO:0032509	endosome transport via multivesicular body sorting pathway	5	11
GO:0032511	late endosome to vacuole transport via multivesicular body sorting pathway	6	11
GO:0033036	macromolecule localization	2	11
GO:0033365	protein localization to organelle	5	11
GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	5	11
GO:0045184	establishment of protein localization	3	11
GO:0045324	late endosome to vacuole transport	5	11
GO:0046907	intracellular transport	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0051649	establishment of localization in cell	3	11
GO:0070727	cellular macromolecule localization	3	11
GO:0071702	organic substance transport	4	11
GO:0071705	nitrogen compound transport	4	11
GO:0071985	multivesicular body sorting pathway	5	11
GO:0072594	establishment of protein localization to organelle	4	11
GO:0072665	protein localization to vacuole	6	11
GO:0072666	establishment of protein localization to vacuole	5	11

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	11
GO:0005515	protein binding	2	11
GO:0005543	phospholipid binding	3	11
GO:0008289	lipid binding	2	11
GO:0032182	ubiquitin-like protein binding	3	11
GO:0032266	phosphatidylinositol-3-phosphate binding	6	11
GO:0035091	phosphatidylinositol binding	4	11
GO:0043130	ubiquitin binding	4	11
GO:1901981	phosphatidylinositol phosphate binding	5	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	357	361	PF00656	0.494
CLV_NRD_NRD_1	200	202	PF00675	0.351
CLV_NRD_NRD_1	326	328	PF00675	0.306
CLV_NRD_NRD_1	69	71	PF00675	0.328
CLV_PCSK_KEX2_1	200	202	PF00082	0.351
CLV_PCSK_KEX2_1	69	71	PF00082	0.328
CLV_PCSK_SKI1_1	327	331	PF00082	0.263
CLV_PCSK_SKI1_1	394	398	PF00082	0.290
CLV_Separin_Metazoa	66	70	PF03568	0.528
DEG_COP1_1	143	153	PF00400	0.532
DEG_Nend_UBRbox_2	1	3	PF02207	0.524
DEG_SPOP_SBC_1	383	387	PF00917	0.487
DOC_CYCLIN_yCln2_LP_2	296	302	PF00134	0.528
DOC_CYCLIN_yCln2_LP_2	61	67	PF00134	0.551
DOC_MAPK_DCC_7	292	302	PF00069	0.528
DOC_MAPK_gen_1	34	44	PF00069	0.534
DOC_MAPK_gen_1	394	404	PF00069	0.523
DOC_MAPK_MEF2A_6	251	260	PF00069	0.528
DOC_PP1_RVXF_1	98	104	PF00149	0.471
DOC_PP2B_LxvP_1	61	64	PF13499	0.462
DOC_USP7_MATH_1	131	135	PF00917	0.653
DOC_USP7_MATH_1	176	180	PF00917	0.522
DOC_USP7_MATH_1	211	215	PF00917	0.551
DOC_USP7_MATH_1	311	315	PF00917	0.496
DOC_USP7_MATH_1	353	357	PF00917	0.398
DOC_USP7_MATH_1	384	388	PF00917	0.489
DOC_USP7_MATH_1	390	394	PF00917	0.479
DOC_USP7_MATH_1	83	87	PF00917	0.511
DOC_WW_Pin1_4	140	145	PF00397	0.715
DOC_WW_Pin1_4	149	154	PF00397	0.637
DOC_WW_Pin1_4	295	300	PF00397	0.478
DOC_WW_Pin1_4	79	84	PF00397	0.478
LIG_14-3-3_CanoR_1	382	390	PF00244	0.523
LIG_BRCT_BRCA1_1	269	273	PF00533	0.551
LIG_BRCT_BRCA1_1	77	81	PF00533	0.551
LIG_CtBP_PxDLS_1	64	68	PF00389	0.551
LIG_deltaCOP1_diTrp_1	2	6	PF00928	0.505
LIG_FHA_1	231	237	PF00498	0.441
LIG_FHA_1	341	347	PF00498	0.528
LIG_FHA_1	439	445	PF00498	0.491
LIG_FHA_1	44	50	PF00498	0.448
LIG_FHA_2	179	185	PF00498	0.478
LIG_FHA_2	218	224	PF00498	0.464
LIG_FHA_2	25	31	PF00498	0.551
LIG_FHA_2	383	389	PF00498	0.551
LIG_FHA_2	431	437	PF00498	0.503
LIG_GBD_Chelix_1	414	422	PF00786	0.262
LIG_LIR_Gen_1	109	119	PF02991	0.445
LIG_LIR_Gen_1	270	281	PF02991	0.551
LIG_LIR_Gen_1	316	324	PF02991	0.527
LIG_LIR_Gen_1	441	447	PF02991	0.422
LIG_LIR_Nem_3	109	115	PF02991	0.452
LIG_LIR_Nem_3	283	288	PF02991	0.448
LIG_LIR_Nem_3	316	321	PF02991	0.466
LIG_LIR_Nem_3	432	438	PF02991	0.445
LIG_LIR_Nem_3	441	445	PF02991	0.378
LIG_LIR_Nem_3	78	84	PF02991	0.526
LIG_PDZ_Class_2	442	447	PF00595	0.366
LIG_RPA_C_Fungi	328	340	PF08784	0.445
LIG_SH2_CRK	112	116	PF00017	0.392
LIG_SH2_CRK	318	322	PF00017	0.413
LIG_SH2_NCK_1	318	322	PF00017	0.413
LIG_SH2_PTP2	98	101	PF00017	0.462
LIG_SH2_STAP1	112	116	PF00017	0.508
LIG_SH2_STAP1	440	444	PF00017	0.479
LIG_SH2_STAT5	427	430	PF00017	0.332
LIG_SH2_STAT5	438	441	PF00017	0.403
LIG_SH2_STAT5	98	101	PF00017	0.389
LIG_SH3_3	147	153	PF00018	0.645
LIG_SH3_3	154	160	PF00018	0.634
LIG_SH3_3	274	280	PF00018	0.301
LIG_SH3_3	318	324	PF00018	0.290
LIG_SH3_3	342	348	PF00018	0.422
LIG_SH3_3	378	384	PF00018	0.376
LIG_SH3_3	88	94	PF00018	0.312
LIG_SUMO_SIM_anti_2	298	304	PF11976	0.445
LIG_SUMO_SIM_anti_2	417	423	PF11976	0.445
LIG_SUMO_SIM_par_1	319	325	PF11976	0.413
LIG_UBA3_1	284	290	PF00899	0.290
LIG_UBA3_1	418	425	PF00899	0.413
MOD_CK1_1	102	108	PF00069	0.472
MOD_CK1_1	140	146	PF00069	0.744
MOD_CK1_1	172	178	PF00069	0.413
MOD_CK1_1	229	235	PF00069	0.384
MOD_CK1_1	245	251	PF00069	0.208
MOD_CK1_1	400	406	PF00069	0.410
MOD_CK1_1	43	49	PF00069	0.435
MOD_CK1_1	76	82	PF00069	0.358
MOD_CK2_1	217	223	PF00069	0.358
MOD_CK2_1	400	406	PF00069	0.369
MOD_Cter_Amidation	325	328	PF01082	0.413
MOD_GlcNHglycan	104	107	PF01048	0.451
MOD_GlcNHglycan	133	136	PF01048	0.668
MOD_GlcNHglycan	139	142	PF01048	0.630
MOD_GlcNHglycan	145	148	PF01048	0.569
MOD_GlcNHglycan	171	174	PF01048	0.324
MOD_GlcNHglycan	313	316	PF01048	0.352
MOD_GlcNHglycan	324	327	PF01048	0.258
MOD_GlcNHglycan	373	376	PF01048	0.362
MOD_GlcNHglycan	70	73	PF01048	0.309
MOD_GlcNHglycan	78	81	PF01048	0.289
MOD_GlcNHglycan	85	88	PF01048	0.274
MOD_GSK3_1	131	138	PF00069	0.695
MOD_GSK3_1	145	152	PF00069	0.681
MOD_GSK3_1	172	179	PF00069	0.349
MOD_GSK3_1	211	218	PF00069	0.339
MOD_GSK3_1	222	229	PF00069	0.249
MOD_GSK3_1	238	245	PF00069	0.246
MOD_GSK3_1	371	378	PF00069	0.365
MOD_GSK3_1	75	82	PF00069	0.272
MOD_N-GLC_1	242	247	PF02516	0.462
MOD_NEK2_1	178	183	PF00069	0.340
MOD_NEK2_1	313	318	PF00069	0.368
MOD_NEK2_1	42	47	PF00069	0.320
MOD_PIKK_1	32	38	PF00454	0.399
MOD_PK_1	354	360	PF00069	0.232
MOD_PKA_1	363	369	PF00069	0.270
MOD_PKA_2	223	229	PF00069	0.430
MOD_PKA_2	68	74	PF00069	0.445
MOD_PKB_1	201	209	PF00069	0.368
MOD_Plk_1	222	228	PF00069	0.311
MOD_Plk_1	230	236	PF00069	0.290
MOD_Plk_1	333	339	PF00069	0.374
MOD_Plk_1	375	381	PF00069	0.396
MOD_Plk_1	400	406	PF00069	0.436
MOD_Plk_1	55	61	PF00069	0.445
MOD_Plk_2-3	223	229	PF00069	0.330
MOD_Plk_2-3	395	401	PF00069	0.368
MOD_Plk_4	24	30	PF00069	0.424
MOD_Plk_4	259	265	PF00069	0.297
MOD_Plk_4	304	310	PF00069	0.403
MOD_Plk_4	333	339	PF00069	0.304
MOD_Plk_4	341	347	PF00069	0.320
MOD_Plk_4	376	382	PF00069	0.377
MOD_Plk_4	417	423	PF00069	0.442
MOD_Plk_4	56	62	PF00069	0.345
MOD_ProDKin_1	140	146	PF00069	0.716
MOD_ProDKin_1	149	155	PF00069	0.637
MOD_ProDKin_1	295	301	PF00069	0.344
MOD_ProDKin_1	79	85	PF00069	0.343
MOD_SUMO_rev_2	2	8	PF00179	0.368
MOD_SUMO_rev_2	393	399	PF00179	0.445
TRG_DiLeu_BaLyEn_6	277	282	PF01217	0.303
TRG_ENDOCYTIC_2	112	115	PF00928	0.427
TRG_ENDOCYTIC_2	318	321	PF00928	0.413
TRG_ENDOCYTIC_2	98	101	PF00928	0.392
TRG_ER_diArg_1	199	201	PF00400	0.445
TRG_ER_diArg_1	68	70	PF00400	0.413
TRG_NES_CRM1_1	395	406	PF08389	0.445
TRG_NES_CRM1_1	56	66	PF08389	0.368
TRG_Pf-PMV_PEXEL_1	200	204	PF00026	0.359
TRG_Pf-PMV_PEXEL_1	254	259	PF00026	0.449

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3P0	Leptomonas seymouri	65%	98%
A0A1X0P698	Trypanosomatidae	44%	99%
A0A3R7M0G6	Trypanosoma rangeli	40%	96%
A0A3S7WVL4	Leishmania donovani	99%	100%
A4HA31	Leishmania braziliensis	77%	100%
A5PK00	Bos taurus	28%	100%
D0A0M8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
E9AS26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
P0C0A2	Rattus norvegicus	28%	100%
Q4QDE7	Leishmania major	94%	100%
Q6DDF4	Xenopus laevis	28%	100%
Q7ZVK4	Danio rerio	28%	100%
Q86VN1	Homo sapiens	28%	100%
Q91XD6	Mus musculus	28%	100%
Q9VU87	Drosophila melanogaster	24%	100%
V5AMS4	Trypanosoma cruzi	43%	99%