Putative GPI-anchor transamidase subunit 8 (GPI8)

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GPI-anchor transamidase subunit 8 (GPI8) - putative

Species:

Leishmania infantum

UniProt:

A4HXR1_LEIIN

TriTrypDb:

LINF_180008600

Length:

356

Annotations

Annotations by Jardim et al.

GPI anchor biosynthesis, GPI-anchor transamidase subunit 8 (GPI8) GPI8

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 6, no: 4
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0008303	caspase complex	5	11
GO:0032991	protein-containing complex	1	11
GO:0042765	GPI-anchor transamidase complex	3	11
GO:0098796	membrane protein complex	2	11
GO:0140534	endoplasmic reticulum protein-containing complex	2	11
GO:1902494	catalytic complex	2	11
GO:1905368	peptidase complex	3	11
GO:1905369	endopeptidase complex	4	11
GO:0005635	nuclear envelope	4	1
GO:0005783	endoplasmic reticulum	5	1
GO:0031967	organelle envelope	3	1
GO:0031975	envelope	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HXR1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HXR1

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0016255	attachment of GPI anchor to protein	5	11
GO:0019538	protein metabolic process	3	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044238	primary metabolic process	2	11
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:0008104	protein localization	4	1
GO:0009987	cellular process	1	1
GO:0033036	macromolecule localization	2	1
GO:0034394	protein localization to cell surface	5	1
GO:0051179	localization	1	1
GO:0051641	cellular localization	2	1
GO:0070727	cellular macromolecule localization	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0003923	GPI-anchor transamidase activity	6	11
GO:0004175	endopeptidase activity	4	11
GO:0004197	cysteine-type endopeptidase activity	5	11
GO:0008233	peptidase activity	3	11
GO:0008234	cysteine-type peptidase activity	4	11
GO:0016787	hydrolase activity	2	11
GO:0140096	catalytic activity, acting on a protein	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	146	148	PF00675	0.470
CLV_NRD_NRD_1	164	166	PF00675	0.398
CLV_NRD_NRD_1	211	213	PF00675	0.460
CLV_NRD_NRD_1	285	287	PF00675	0.625
CLV_PCSK_KEX2_1	146	148	PF00082	0.470
CLV_PCSK_KEX2_1	164	166	PF00082	0.398
CLV_PCSK_KEX2_1	211	213	PF00082	0.473
CLV_PCSK_KEX2_1	285	287	PF00082	0.602
CLV_PCSK_SKI1_1	146	150	PF00082	0.459
CLV_PCSK_SKI1_1	164	168	PF00082	0.465
CLV_PCSK_SKI1_1	184	188	PF00082	0.326
CLV_PCSK_SKI1_1	215	219	PF00082	0.456
CLV_PCSK_SKI1_1	63	67	PF00082	0.473
DEG_APCC_DBOX_1	145	153	PF00400	0.273
DOC_CYCLIN_RxL_1	161	170	PF00134	0.339
DOC_MAPK_gen_1	211	219	PF00069	0.259
DOC_MAPK_HePTP_8	339	351	PF00069	0.305
DOC_MAPK_MEF2A_6	171	178	PF00069	0.279
DOC_MAPK_MEF2A_6	342	351	PF00069	0.378
DOC_PP1_RVXF_1	213	219	PF00149	0.274
DOC_PP4_FxxP_1	112	115	PF00568	0.329
DOC_USP7_MATH_1	38	42	PF00917	0.466
DOC_USP7_MATH_2	108	114	PF00917	0.307
DOC_USP7_UBL2_3	184	188	PF12436	0.339
DOC_WW_Pin1_4	157	162	PF00397	0.273
LIG_14-3-3_CanoR_1	146	152	PF00244	0.332
LIG_14-3-3_CanoR_1	164	174	PF00244	0.179
LIG_14-3-3_CanoR_1	6	16	PF00244	0.507
LIG_14-3-3_CanoR_1	69	77	PF00244	0.258
LIG_BIR_III_4	120	124	PF00653	0.237
LIG_BRCT_BRCA1_1	95	99	PF00533	0.339
LIG_deltaCOP1_diTrp_1	332	338	PF00928	0.344
LIG_FHA_1	161	167	PF00498	0.334
LIG_FHA_1	190	196	PF00498	0.329
LIG_FHA_1	258	264	PF00498	0.307
LIG_FHA_1	282	288	PF00498	0.421
LIG_FHA_1	8	14	PF00498	0.445
LIG_LIR_Apic_2	110	115	PF02991	0.297
LIG_LIR_Gen_1	26	37	PF02991	0.399
LIG_LIR_Gen_1	52	59	PF02991	0.258
LIG_LIR_Gen_1	76	86	PF02991	0.339
LIG_LIR_LC3C_4	10	15	PF02991	0.340
LIG_LIR_Nem_3	185	189	PF02991	0.267
LIG_LIR_Nem_3	26	32	PF02991	0.395
LIG_LIR_Nem_3	269	274	PF02991	0.300
LIG_LIR_Nem_3	298	302	PF02991	0.281
LIG_LIR_Nem_3	52	58	PF02991	0.250
LIG_LIR_Nem_3	76	81	PF02991	0.302
LIG_REV1ctd_RIR_1	184	192	PF16727	0.209
LIG_SH2_CRK	213	217	PF00017	0.273
LIG_SH2_CRK	271	275	PF00017	0.290
LIG_SH2_CRK	29	33	PF00017	0.400
LIG_SH2_CRK	78	82	PF00017	0.339
LIG_SH2_STAP1	250	254	PF00017	0.289
LIG_SH2_STAP1	29	33	PF00017	0.459
LIG_SH2_STAT3	250	253	PF00017	0.329
LIG_SH2_STAT3	254	257	PF00017	0.329
LIG_SH2_STAT5	177	180	PF00017	0.291
LIG_SH2_STAT5	64	67	PF00017	0.259
LIG_SH3_3	229	235	PF00018	0.362
LIG_SH3_3	321	327	PF00018	0.335
LIG_SUMO_SIM_anti_2	10	17	PF11976	0.340
LIG_SUMO_SIM_anti_2	290	296	PF11976	0.373
LIG_SUMO_SIM_par_1	14	20	PF11976	0.313
LIG_WRC_WIRS_1	262	267	PF05994	0.292
MOD_CDK_SPxxK_3	157	164	PF00069	0.273
MOD_CK1_1	198	204	PF00069	0.362
MOD_CK1_1	23	29	PF00069	0.633
MOD_CK2_1	240	246	PF00069	0.312
MOD_GlcNHglycan	132	135	PF01048	0.514
MOD_GlcNHglycan	40	43	PF01048	0.705
MOD_GSK3_1	165	172	PF00069	0.265
MOD_GSK3_1	189	196	PF00069	0.273
MOD_GSK3_1	19	26	PF00069	0.398
MOD_GSK3_1	240	247	PF00069	0.313
MOD_GSK3_1	257	264	PF00069	0.328
MOD_GSK3_1	277	284	PF00069	0.243
MOD_GSK3_1	309	316	PF00069	0.466
MOD_N-GLC_1	274	279	PF02516	0.567
MOD_N-GLC_1	313	318	PF02516	0.614
MOD_NEK2_1	17	22	PF00069	0.427
MOD_NEK2_1	219	224	PF00069	0.260
MOD_NEK2_1	274	279	PF00069	0.349
MOD_NEK2_1	295	300	PF00069	0.289
MOD_NEK2_1	44	49	PF00069	0.460
MOD_NEK2_1	95	100	PF00069	0.288
MOD_NEK2_2	182	187	PF00069	0.251
MOD_PIKK_1	110	116	PF00454	0.307
MOD_PK_1	147	153	PF00069	0.339
MOD_PKA_2	5	11	PF00069	0.479
MOD_PKA_2	68	74	PF00069	0.270
MOD_Plk_4	147	153	PF00069	0.314
MOD_Plk_4	182	188	PF00069	0.289
MOD_Plk_4	200	206	PF00069	0.169
MOD_Plk_4	261	267	PF00069	0.304
MOD_Plk_4	269	275	PF00069	0.305
MOD_Plk_4	290	296	PF00069	0.347
MOD_Plk_4	73	79	PF00069	0.273
MOD_Plk_4	8	14	PF00069	0.412
MOD_ProDKin_1	157	163	PF00069	0.273
MOD_SUMO_rev_2	168	172	PF00179	0.279
TRG_DiLeu_BaEn_1	269	274	PF01217	0.291
TRG_DiLeu_BaLyEn_6	162	167	PF01217	0.339
TRG_ENDOCYTIC_2	213	216	PF00928	0.273
TRG_ENDOCYTIC_2	271	274	PF00928	0.315
TRG_ENDOCYTIC_2	29	32	PF00928	0.400
TRG_ENDOCYTIC_2	78	81	PF00928	0.339
TRG_ER_diArg_1	145	147	PF00400	0.271
TRG_ER_diArg_1	164	166	PF00400	0.174
TRG_ER_diArg_1	211	213	PF00400	0.273
TRG_ER_diArg_1	285	287	PF00400	0.425
TRG_NES_CRM1_1	256	269	PF08389	0.303
TRG_Pf-PMV_PEXEL_1	164	169	PF00026	0.459

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJ31	Leptomonas seymouri	81%	98%
A0A1X0P6S3	Trypanosomatidae	54%	100%
A0A3S5H727	Leishmania donovani	99%	100%
A4H9E0	Leishmania braziliensis	82%	100%
B6DTF5	Bodo saltans	39%	100%
D0A043	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	100%
E9ARH3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
O24326	Phaseolus vulgaris	23%	72%
O89017	Mus musculus	27%	82%
P49048	Caenorhabditis elegans	37%	100%
Q4QE06	Leishmania major	91%	100%
Q4R4T8	Macaca fascicularis	26%	82%
Q5R5D9	Pongo abelii	27%	82%
Q8T4E1	Drosophila melanogaster	35%	100%
Q92643	Homo sapiens	39%	90%
Q95M12	Bos taurus	27%	82%
Q99538	Homo sapiens	27%	82%
Q9USP5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	37%	94%
V5BWA3	Trypanosoma cruzi	54%	100%