Ubiquitin thioesterase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

otubain cysteine peptidase - Clan CA - family C65 - putative

Species:

Leishmania infantum

UniProt:

A4HXL8_LEIIN

TriTrypDb:

LINF_170021400

Length:

270

Annotations

Annotations by Jardim et al.

Protein modification, otubain

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	yes	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 8

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	4
GO:0043226	organelle	2	4
GO:0043227	membrane-bounded organelle	3	4
GO:0043229	intracellular organelle	3	4
GO:0043231	intracellular membrane-bounded organelle	4	4
GO:0110165	cellular anatomical entity	1	4

Expansion

Sequence features

A4HXL8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HXL8

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008152	metabolic process	1	10
GO:0016579	protein deubiquitination	6	6
GO:0019538	protein metabolic process	3	10
GO:0036211	protein modification process	4	6
GO:0043170	macromolecule metabolic process	3	10
GO:0043412	macromolecule modification	4	6
GO:0044238	primary metabolic process	2	10
GO:0070646	protein modification by small protein removal	5	6
GO:0070647	protein modification by small protein conjugation or removal	5	6
GO:0071108	protein K48-linked deubiquitination	7	4
GO:0071704	organic substance metabolic process	2	10
GO:1901564	organonitrogen compound metabolic process	3	10

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0004843	cysteine-type deubiquitinase activity	5	10
GO:0005488	binding	1	4
GO:0005515	protein binding	2	4
GO:0008233	peptidase activity	3	10
GO:0008234	cysteine-type peptidase activity	4	10
GO:0016787	hydrolase activity	2	10
GO:0019783	ubiquitin-like protein peptidase activity	4	10
GO:0032182	ubiquitin-like protein binding	3	4
GO:0043130	ubiquitin binding	4	4
GO:0101005	deubiquitinase activity	5	10
GO:0140096	catalytic activity, acting on a protein	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	125	129	PF00656	0.385
CLV_C14_Caspase3-7	243	247	PF00656	0.489
CLV_C14_Caspase3-7	75	79	PF00656	0.385
CLV_NRD_NRD_1	18	20	PF00675	0.517
CLV_NRD_NRD_1	76	78	PF00675	0.309
CLV_PCSK_KEX2_1	18	20	PF00082	0.471
CLV_PCSK_KEX2_1	76	78	PF00082	0.309
CLV_PCSK_SKI1_1	140	144	PF00082	0.405
DEG_Nend_UBRbox_2	1	3	PF02207	0.693
DOC_MAPK_MEF2A_6	216	224	PF00069	0.309
DOC_PP2B_LxvP_1	256	259	PF13499	0.425
DOC_PP2B_LxvP_1	29	32	PF13499	0.432
DOC_USP7_MATH_1	181	185	PF00917	0.336
DOC_WW_Pin1_4	57	62	PF00397	0.408
LIG_14-3-3_CanoR_1	151	160	PF00244	0.401
LIG_BRCT_BRCA1_1	115	119	PF00533	0.385
LIG_Clathr_ClatBox_1	121	125	PF01394	0.336
LIG_DLG_GKlike_1	111	118	PF00625	0.385
LIG_FHA_1	125	131	PF00498	0.373
LIG_FHA_1	51	57	PF00498	0.421
LIG_FHA_2	188	194	PF00498	0.415
LIG_LIR_Gen_1	103	108	PF02991	0.440
LIG_LIR_Gen_1	68	74	PF02991	0.324
LIG_LIR_LC3C_4	248	253	PF02991	0.425
LIG_LIR_Nem_3	103	107	PF02991	0.387
LIG_LIR_Nem_3	116	122	PF02991	0.257
LIG_LIR_Nem_3	176	182	PF02991	0.385
LIG_LIR_Nem_3	68	72	PF02991	0.342
LIG_LYPXL_yS_3	24	27	PF13949	0.336
LIG_Pex14_2	122	126	PF04695	0.309
LIG_Pex14_2	214	218	PF04695	0.333
LIG_RPA_C_Fungi	106	118	PF08784	0.385
LIG_SH2_CRK	188	192	PF00017	0.425
LIG_SH2_CRK	262	266	PF00017	0.309
LIG_SH2_PTP2	104	107	PF00017	0.385
LIG_SH2_SRC	166	169	PF00017	0.436
LIG_SH2_STAT3	49	52	PF00017	0.425
LIG_SH2_STAT5	104	107	PF00017	0.368
LIG_SH2_STAT5	159	162	PF00017	0.315
LIG_SH2_STAT5	166	169	PF00017	0.300
LIG_SH2_STAT5	171	174	PF00017	0.295
LIG_SH2_STAT5	194	197	PF00017	0.425
LIG_SH2_STAT5	49	52	PF00017	0.425
LIG_SH3_3	92	98	PF00018	0.425
LIG_SUMO_SIM_anti_2	103	110	PF11976	0.385
LIG_SUMO_SIM_anti_2	198	203	PF11976	0.403
LIG_SUMO_SIM_par_1	25	30	PF11976	0.381
LIG_TRAF2_1	110	113	PF00917	0.336
LIG_TYR_ITIM	186	191	PF00017	0.309
MOD_CK1_1	60	66	PF00069	0.385
MOD_CK2_1	107	113	PF00069	0.336
MOD_GlcNHglycan	168	171	PF01048	0.408
MOD_GlcNHglycan	183	186	PF01048	0.451
MOD_GSK3_1	107	114	PF00069	0.369
MOD_GSK3_1	57	64	PF00069	0.405
MOD_GSK3_1	68	75	PF00069	0.474
MOD_N-GLC_1	233	238	PF02516	0.399
MOD_N-GLC_2	219	221	PF02516	0.425
MOD_NEK2_1	27	32	PF00069	0.345
MOD_NEK2_1	50	55	PF00069	0.458
MOD_NEK2_2	187	192	PF00069	0.398
MOD_PKA_2	72	78	PF00069	0.393
MOD_Plk_1	111	117	PF00069	0.385
MOD_Plk_1	138	144	PF00069	0.385
MOD_Plk_1	236	242	PF00069	0.425
MOD_Plk_4	34	40	PF00069	0.330
MOD_Plk_4	52	58	PF00069	0.338
MOD_ProDKin_1	57	63	PF00069	0.408
TRG_DiLeu_BaEn_1	103	108	PF01217	0.385
TRG_DiLeu_BaEn_3	177	183	PF01217	0.336
TRG_DiLeu_BaLyEn_6	22	27	PF01217	0.490
TRG_ENDOCYTIC_2	104	107	PF00928	0.445
TRG_ENDOCYTIC_2	149	152	PF00928	0.425
TRG_ENDOCYTIC_2	179	182	PF00928	0.385
TRG_ENDOCYTIC_2	188	191	PF00928	0.273
TRG_ENDOCYTIC_2	24	27	PF00928	0.457
TRG_ENDOCYTIC_2	262	265	PF00928	0.309
TRG_ER_diArg_1	17	19	PF00400	0.475
TRG_NES_CRM1_1	113	125	PF08389	0.336
TRG_Pf-PMV_PEXEL_1	151	155	PF00026	0.494

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1P6	Leptomonas seymouri	63%	99%
A0A0S4J6F9	Bodo saltans	33%	100%
A0A1X0NSN1	Trypanosomatidae	36%	100%
A0A3S7WUP0	Leishmania donovani	100%	100%
A0A422NBK6	Trypanosoma rangeli	40%	100%
A4H997	Leishmania braziliensis	83%	100%
A8XDJ2	Caenorhabditis briggsae	33%	94%
B2RYG6	Rattus norvegicus	33%	100%
C9ZPB3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
E9ARB4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
Q4QE49	Leishmania major	98%	100%
Q7TQI3	Mus musculus	33%	100%
Q8LG98	Arabidopsis thaliana	31%	88%
Q96DC9	Homo sapiens	26%	100%
Q96FW1	Homo sapiens	33%	100%
Q9CQX0	Mus musculus	28%	100%
Q9VL00	Drosophila melanogaster	28%	100%
Q9XVR6	Caenorhabditis elegans	29%	95%
V5BNY1	Trypanosoma cruzi	39%	100%