Putative replication Factor A 28 kDa subunit

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

replication Factor A 28 kDa subunit - putative

Species:

Leishmania infantum

UniProt:

A4HWC8_LEIIN

TriTrypDb:

LINF_150008000

Length:

257

Annotations

Annotations by Jardim et al.

DNA replication, replication Factor A 28 kDa subunit

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 25

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	6
GO:0043226	organelle	2	6
GO:0043227	membrane-bounded organelle	3	6
GO:0043229	intracellular organelle	3	6
GO:0043231	intracellular membrane-bounded organelle	4	6
GO:0110165	cellular anatomical entity	1	6
GO:0000781	chromosome, telomeric region	3	1
GO:0005654	nucleoplasm	2	1
GO:0005662	DNA replication factor A complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0035861	site of double-strand break	3	1
GO:0090734	site of DNA damage	2	1
GO:0098687	chromosomal region	2	1
GO:0140513	nuclear protein-containing complex	2	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A4HWC8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HWC8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	6
GO:0006259	DNA metabolic process	4	6
GO:0006260	DNA replication	5	6
GO:0006281	DNA repair	5	6
GO:0006310	DNA recombination	5	6
GO:0006725	cellular aromatic compound metabolic process	3	6
GO:0006807	nitrogen compound metabolic process	2	6
GO:0006950	response to stress	2	6
GO:0006974	DNA damage response	4	6
GO:0008152	metabolic process	1	6
GO:0009987	cellular process	1	6
GO:0033554	cellular response to stress	3	6
GO:0034641	cellular nitrogen compound metabolic process	3	6
GO:0043170	macromolecule metabolic process	3	6
GO:0044237	cellular metabolic process	2	6
GO:0044238	primary metabolic process	2	6
GO:0044260	obsolete cellular macromolecule metabolic process	3	6
GO:0046483	heterocycle metabolic process	3	6
GO:0050896	response to stimulus	1	6
GO:0051716	cellular response to stimulus	2	6
GO:0071704	organic substance metabolic process	2	6
GO:0090304	nucleic acid metabolic process	4	6
GO:1901360	organic cyclic compound metabolic process	3	6
GO:0000724	double-strand break repair via homologous recombination	7	1
GO:0000725	recombinational repair	6	1
GO:0006289	nucleotide-excision repair	6	1
GO:0006302	double-strand break repair	6	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	7
GO:0003677	DNA binding	4	6
GO:0005488	binding	1	7
GO:0097159	organic cyclic compound binding	2	7
GO:1901363	heterocyclic compound binding	2	7
GO:0003697	single-stranded DNA binding	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	140	144	PF00656	0.476
CLV_NRD_NRD_1	27	29	PF00675	0.591
CLV_PCSK_KEX2_1	166	168	PF00082	0.301
CLV_PCSK_KEX2_1	27	29	PF00082	0.591
CLV_PCSK_PC1ET2_1	166	168	PF00082	0.350
CLV_PCSK_SKI1_1	194	198	PF00082	0.350
CLV_PCSK_SKI1_1	33	37	PF00082	0.356
CLV_PCSK_SKI1_1	38	42	PF00082	0.357
CLV_PCSK_SKI1_1	56	60	PF00082	0.421
DEG_Nend_Nbox_1	1	3	PF02207	0.611
DOC_MAPK_DCC_7	27	37	PF00069	0.483
DOC_USP7_MATH_1	13	17	PF00917	0.625
DOC_USP7_MATH_1	213	217	PF00917	0.350
DOC_WW_Pin1_4	2	7	PF00397	0.658
LIG_14-3-3_CanoR_1	226	234	PF00244	0.350
LIG_14-3-3_CanoR_1	90	98	PF00244	0.485
LIG_FHA_1	122	128	PF00498	0.485
LIG_FHA_1	133	139	PF00498	0.485
LIG_FHA_1	196	202	PF00498	0.350
LIG_FHA_1	93	99	PF00498	0.455
LIG_FHA_2	228	234	PF00498	0.350
LIG_LIR_Gen_1	146	156	PF02991	0.431
LIG_LIR_LC3C_4	95	100	PF02991	0.485
LIG_LIR_Nem_3	146	152	PF02991	0.434
LIG_NRBOX	196	202	PF00104	0.350
LIG_PDZ_Class_2	252	257	PF00595	0.463
LIG_SH2_GRB2like	70	73	PF00017	0.516
LIG_SH2_NCK_1	70	74	PF00017	0.474
LIG_SH2_SRC	70	73	PF00017	0.516
LIG_SH3_1	28	34	PF00018	0.498
LIG_SH3_3	28	34	PF00018	0.498
LIG_SUMO_SIM_par_1	49	54	PF11976	0.423
LIG_WRC_WIRS_1	201	206	PF05994	0.350
MOD_CK1_1	17	23	PF00069	0.644
MOD_CK1_1	171	177	PF00069	0.363
MOD_CK2_1	213	219	PF00069	0.350
MOD_GlcNHglycan	121	124	PF01048	0.285
MOD_GlcNHglycan	170	173	PF01048	0.355
MOD_GlcNHglycan	179	182	PF01048	0.359
MOD_GlcNHglycan	19	22	PF01048	0.643
MOD_GlcNHglycan	210	213	PF01048	0.423
MOD_GlcNHglycan	222	225	PF01048	0.251
MOD_GlcNHglycan	76	79	PF01048	0.246
MOD_GSK3_1	1	8	PF00069	0.589
MOD_GSK3_1	13	20	PF00069	0.556
MOD_GSK3_1	137	144	PF00069	0.548
MOD_GSK3_1	167	174	PF00069	0.294
MOD_GSK3_1	233	240	PF00069	0.350
MOD_N-GLC_1	17	22	PF02516	0.644
MOD_NEK2_1	1	6	PF00069	0.597
MOD_NEK2_1	159	164	PF00069	0.350
MOD_NEK2_1	200	205	PF00069	0.350
MOD_NEK2_1	220	225	PF00069	0.122
MOD_PIKK_1	159	165	PF00454	0.350
MOD_PIKK_1	56	62	PF00454	0.409
MOD_PKA_2	119	125	PF00069	0.485
MOD_PKA_2	159	165	PF00069	0.350
MOD_PKA_2	89	95	PF00069	0.485
MOD_Plk_4	134	140	PF00069	0.485
MOD_Plk_4	200	206	PF00069	0.350
MOD_Plk_4	59	65	PF00069	0.328
MOD_ProDKin_1	2	8	PF00069	0.656
TRG_ENDOCYTIC_2	149	152	PF00928	0.435
TRG_ER_diArg_1	27	29	PF00400	0.651
TRG_Pf-PMV_PEXEL_1	239	244	PF00026	0.292
TRG_Pf-PMV_PEXEL_1	66	71	PF00026	0.354

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2B8	Leptomonas seymouri	89%	100%
A0A1X0NTN7	Trypanosomatidae	62%	100%
A0A3Q8IDB6	Leishmania donovani	100%	100%
A0A3S5IRR4	Trypanosoma rangeli	63%	96%
A1L2H9	Xenopus laevis	26%	94%
A4H800	Leishmania braziliensis	92%	100%
E9AQ28	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
P15927	Homo sapiens	25%	95%
Q23697	Crithidia fasciculata	85%	100%
Q4QFF3	Leishmania major	98%	100%
Q5RC43	Pongo abelii	25%	95%
Q62193	Mus musculus	25%	95%
Q63528	Rattus norvegicus	25%	95%
Q6DFS2	Xenopus tropicalis	25%	93%
Q6IP18	Xenopus laevis	26%	93%
Q6K9U2	Oryza sativa subsp. japonica	25%	92%
Q8LFJ8	Arabidopsis thaliana	22%	92%